Re: [Histonet] cleaning old charged slides

[Atoska Gentry via Histonet]

Hello,

Has anyone had success cleaning old charged slides which have become foggy over 
time.

Thanks!
~Atoska
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Re: [Histonet] Leitz Rotary Microtome

[Atoska Gentry via Histonet]

Hello,
Does anyone have experience with a Leitz Minot Rotary Microtome No. 1212?
I'm particularly interested in a source for troubleshooting problem sectioning.
Thanking you in advance for your assistance.

Best Regards,
~Atoska
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Re: [Histonet] Histonet Digest, Vol 188, Issue 17

[Atoska Gentry via Histonet]

Hello Sandra, 

Following are catalog #'s for Fisher substitutes that I received from their 
site: 12-545GP (50x35mm); 12-548-5RP (45x50mm). 
Ted Pella, Inc.  Gold Seal 3327
www.expressmedicalsupply.com Erie-Scientific ER 35x50-1.5 &  ER 45x50-1
Also try Brain Research Laboratories 4860-1 (48x60mm) although they're a bit 
more pricey.
Thomas Scientific (48x60mm) #6672-A64
Additionally, I've seen some eBay listings.

Best wishes,

~Atoska

-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu 
 
Sent: Wednesday, July 24, 2019 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 188, Issue 17

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Today's Topics:

   1. Jeepers Creepers, Coverslipping Peepers! (Sandra Cheasty)
   2. Re: [External]  Jeepers Creepers, Coverslipping Peepers!
  (Dessasau III, Evan)
   3. Two part Special Stain wash (Cassie P. Davis)


--

Message: 1
Date: Tue, 23 Jul 2019 19:49:21 +
From: Sandra Cheasty 
To: "Histonet (histonet@lists.utsouthwestern.edu)"

Subject: [Histonet] Jeepers Creepers, Coverslipping Peepers!
Message-ID:



Content-Type: text/plain; charset="us-ascii"

Hello all,
Does anyone have a source for oversized coverglass? (35x50, 
35x60, 45x50, 45x60) We section a lot of globes from the ophthalmology lab, 
some of which are from horses, cows, etc. that are put on the larger slides. 
Fisher used to carry their own brand, but it's apparently discontinued them, 
and their "alternate" product is $50/oz!
Thanks!
Sandy

Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary Medicine



--

Message: 2
Date: Tue, 23 Jul 2019 20:46:38 +
From: "Dessasau III, Evan" 
To: Sandra Cheasty 
Cc: "'histonet@lists.utsouthwestern.edu'"

Subject: Re: [Histonet] [External]  Jeepers Creepers, Coverslipping
Peepers!
Message-ID:



Content-Type: text/plain; charset="us-ascii"

Hi Sandra, Have you tried Brain Research Laboratories?  We use the 35x50mm 
no.1, 3oz. pack.  We buy them through Mark at PathSupply.  The phone number on 
the box is 617 965 5544.  PathSupply's number is 800 631 3556.
Thank you,

E-van

-Original Message-
From: Sandra Cheasty via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, July 23, 2019 3:49 PM
To: Histonet (histonet@lists.utsouthwestern.edu) 

Subject: [External] [Histonet] Jeepers Creepers, Coverslipping Peepers!

Hello all,
Does anyone have a source for oversized coverglass? (35x50, 
35x60, 45x50, 45x60) We section a lot of globes from the ophthalmology lab, 
some of which are from horses, cows, etc. that are put on the larger slides. 
Fisher used to carry their own brand, but it's apparently discontinued them, 
and their "alternate" product is $50/oz!
Thanks!
Sandy

Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary Medicine

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--

Message: 3
Date: Wed, 24 Jul 2019 16:41:14 +
From: "Cassie P. Davis" 
To: histonet 
Subject: [Histonet] Two part Special Stain wash
Message-ID: 
Content-Type: text/plain; charset="iso-8859-1"

Hi Histonet folks,

  If you still have the NEXES special stainer do you use the two part wash  
cat#860-040 or the 10x concentrate cat#860-015?

Cassandra Davis
Histology Technician
AP Laboratory
302-575-8095
Email:  cda...@che-east.org



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intended for the sole use of the intended recipient(s). It may contain 
information that is privileged and 

[Histonet] mouse pituitary

[Atoska Gentry via Histonet]

Hello,

does anyone have a tried & proven method and/or pointers  for mounting mouse 
pituitary for cryo sectioning which guarantees great cross sections?

Thanks!

~Atoska

Sent from Mail for Windows 10

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Re: [Histonet] blades

[Atoska Gentry via Histonet]

Hello does anyone have a source for Personna Single-edge stainless steel razor 
blades? Our former vendor no longer stocks them. Thanks, Atoska
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Re: [Histonet] specimen storage cabinets

[Atoska Gentry via Histonet]

Hello, I work for a research facility and we currently have an archive of 
several formalin fixed samples stored in 500ml glass specimen jars; one of our 
PI's recently asked me to conduct a search for storage cabinets specifically 
designed to accommodate these samples. Is anyone aware of the availability of  
such cabinets? The only thing my search has turned up thus far are the 
flammable metal cabinets. But due to limited storage space we have to stack 
these jars and the generic flammable cabinets are not stacked glass specimen 
jar suitable. All ideas & input are welcome. Thank you kindly, Atoska
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Re: [Histonet] Automated Slide Stainers

[Atoska Gentry via Histonet]

Hello, one of my department's researchers is in need of an automated slide 
stainer which accommodates 3"x2" microscope slides. If you have experience with 
such will you please contact me at your earliest convenience? Regards, Atoska 
Gentry
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Re: [Histonet] microscope slides

[Atoska Gentry]

Good afternoon, does cleaning dust from adhesion superfrost plus microscope 
slides interfere with paraffin section adhesion? If not will someone be so kind 
as to share their  tried and proven method ASAP? Thanks in advance. ~ Atoska
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[Histonet] RE: Hemo-De

[Atoska Gentry]

Hello is  anyone using  Hemo=De as the clearing agent in the following stains: 
Bielschowsky's Method for Axis Cylinders  Dendrites and Sevier-Munger Method 
for Neural Tissues? Regards. Atoska
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[Histonet] RE: Hemo-De

[Atoska Gentry]

Hello, has anyone use or are you currently using Hemo-De as the clearing agent 
in these stains: 1) Bielschowsky's Method for Axis Cylinders and Dendrites 
and/or 2) Sevier-Munger Method for Neural Tissues? Your prompt reply will be 
much appreciated.  Regards. ~ Atoska
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[Histonet] RE: Adipose Cryosections Fixation

[Atoska Gentry]

hello! Will you either provide me with info  protocol for fixation of
adipose cryosections prior to staining and/or contact info for Dr. M.
Rocio Sierra-Honigmann ASAP? Thank you kindly, Atoska

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[Histonet] RE: Feline adipose tissue IHC

[Atoska Gentry]

hello, I'm assisting a graduate student who's interested in info on
source recommendations for the following macrophage markers which 
guarantee results on feline tissue: 1) AM3-K, 2) alpha1-antitrypsin  and
3) Lysozyme. Your prompt replies with pertinent info will be greatly
appreciated. ~ Atoska

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[Histonet] RE: Microm HM 505 E Cryostat

[Atoska Gentry]

hello, has anyone with this particular cryostat experienced frost build
up on the top interior area located above the microtome? I've also
noticed the temperature has for the past couple of weeks been reading ~
3 - 4 C colder than temperature setting. Thus far it seems to still be
sectioning just fine. I'm just curious to know if this is an indication
of a malfunction of some sort? I also need to mention that on  Monday
morning  I also observed quite a bit of water on the floor of the lab
where this instrument is housed  and it's source has yet to be
determined. Any pointers will be much appreciated.

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[Histonet] Re: replacement Turtox Internal Ear, cochlea slides

[Atoska Gentry]

Hello! please see below inquiry from a colleague. Thanks! Atoska


Our histology class is looking for replacements for old Turtox (Guinea
Pig) Internal Ear, cochlea slides and was wondering if anyone knew a
company who supplied these slides or tissue blocks? I don't think it has
to be guinea pig but I haven't been able to locate internal ear slides
from any species.  Any help would be appreciated.  Thanks!

~~~
Michelle (Shelly) Aono
Histology Technician IV
CVM  AU Staff 
Advisory Council Representative
Auburn University
CVM-APP
212 Greene Hall, Auburn, AL 36832
(334) 844-5594


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[Histonet] RE: Lynn Burton, Galesburg, IL

[Atoska Gentry]

Hello Lynn Burton of Animal Disease Lab, Galesburg, IL will you please
contact me ASAP regarding a 2006 Histonet posting.

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[Histonet] Re: chick embryo frozen sections

[Atoska Gentry]

hello, will some one please give me pointers on successful methods used
for  cryosectioning bursa  esophagus from approx. 21 day chick embryos
(such as recommended temp.  micron thickness, for yielding the best
sections)? Thanks! Atoska

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[Histonet] Re: Acetone Fixation

[Atoska Gentry]

hello, if any of you have acetone fixation incorporated into you frozen
section H E staining protocol will you please advise me on adjustments
necessary for routine staining protocol. Also, out of curiosity please
enlighten me on the purpose for post sectioning acetone fixation on
tissue samples  initially fixed in   95% ETOH/ Acetic Acid? Your prompt
replies will be greatly appreciated. ~ Atoska

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[Histonet] Re: tissue processor

[Atoska Gentry]

hello, I'm in search of the best tissue processor on the market thats' 
most comparable to the Autotechnicon Ultra II. I specifically need it to 
be  equipped with vacuum  heat controlled processing and for it to 
possess the capability of processing 2.75 and/or 1.5 stainless steel 
round tissue cassettes.   Prompt reply will be much appreciated. ~ Atoska


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[Histonet] Re: Citadel 2000 Tissue Processor

[Atoska Gentry]

Hello, will everyone who's used/using the Citadel 2000 Tissue Processor 
please share with me your opinions, pros/cons and etc ASAP? Thanks! Atoska


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[Histonet] Re: Cynthia Favara

[Atoska Gentry]

hello will someone please provide me with contact info for Cynthia 
Favara ASAP? I need to consult with her on a histonet archive post. 
Thanks! Atoska


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[Histonet] Re: heartworm processing protocol

[Atoska Gentry]

hello, will someone with a tried  proven protocol for processing  
heartworms for paraffin sectioning please share your protocol with me 
ASAP? My histonet archive search was unsuccessful. Thanks! Atoska


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[Histonet] RE: Tissue Capture Pen

[Atoska Gentry]

hello, if any of you are using and/or have used the Ted Pella, Inc. 
Tissue Capture Pen will you be so kind as to share with me your 
experience (s), pros, cons and whatever else you deem necessary? Also, 
does it's use require special glass slides? Any info you can provide on 
it's use ASAP will be much appreciated. Thank you kindly, Atoska


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[Histonet] Re: tissue processors

[Atoska Gentry]

hello, we are in the market for a new/replacement tissue processor. If 
you have experience and/or pertinent detailed info on any of the 
following  will you please share with me ASAP? *1.*ThermoShandon Citadel 
2000 Tissue Processor, *2.* Leica TP 1020 Automatic Tissue Processor, 
and last but not least  *3.* VIP 1000 Floor Tissue Processor. Thank you 
kindly. Atoska

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