Hi Jean,
We use Cell Marque's (via VMS) clone 24. We have to throw the entire
arsenal at our disposal at these stains but they're acceptable.
Good luck Jean; any questions, you know where we live!
Linda
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Taylor,
Jean
Sent: Thursday, August 19, 2010 9:45 AM
To: 'histonet@lists.utsouthwestern.edu'; 'ih...@googlegroups.com'
Subject: [Histonet] PAX-5 - B-cell specific activator protein (BSAP)
Hi Everyone,
I'm trying to find out what company and clone most labs are using for
PAX-5 (BSAP).
Thanks!
Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
Madison, WI
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histonet-requ...@lists.utsouthwestern.edu
Sent: Wednesday, August 18, 2010 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 81, Issue 23
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Re: Contents of Histonet digest...
Today's Topics:
1. RE: CD34 positive control (McMahon, Loralee A)
2. Ventana Renaissance Containers (ricky hachy)
3. CD34 Control (Silverman, Jeffrey)
--
Message: 1
Date: Wed, 18 Aug 2010 12:17:39 -0400
From: McMahon, Loralee A loralee_mcma...@urmc.rochester.edu
Subject: RE: [Histonet] CD34 positive control
To: Joel Reichensperger jreichensper...@siumed.edu, Histonet
histonet@lists.utsouthwestern.edu
Message-ID:
c27aa2a01cef31469813089e226f582e02d5a7c...@urmcms7.urmc-sh.rochester.ed
u
Content-Type: text/plain; charset=us-ascii
Just about any tissue should work. It stains for Hematopoietic
stem/progenitor cells, bone marrow stromal cells, endothelial cells,
embryonic fibroblasts
We use a sausage style control here with about 30 different types of
tissue.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joel
Reichensperger [jreichensper...@siumed.edu]
Sent: Wednesday, August 18, 2010 12:05 PM
To: Histonet
Subject: [Histonet] CD34 positive control
I have a doctor who wants to stain some tissue with cd34. I need to
know if anyone can recommend a good positive control tissue for this
antibody. The staining will be done in paraffin embedded sections.
Thanks in advance.
Joel
--
Joel Reichensperger
Researcher II
Southern Illinois University
Plastic Surgery Institute
jreichensper...@siumed.edu
217-545-7309 (Office)
217-545-1824 (Fax)
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Message: 2
Date: Wed, 18 Aug 2010 16:28:39 +
From: ricky hachy elc...@hotmail.com
Subject: [Histonet] Ventana Renaissance Containers
To: Histonet histonet@lists.utsouthwestern.edu
Message-ID: snt114-w54ab5f08e7e0cfc97193edcf...@phx.gbl
Content-Type: text/plain; charset=iso-8859-1
Hello ,
I need 4 good plastic containers, where you fill with
xilene,alcool... for the VENTANA RENAISSANCE .
Anyone could sell me ?
Regards
Ricky
--
Message: 3
Date: Wed, 18 Aug 2010 12:30:58 -0400
From: Silverman, Jeffrey jsilver...@nshs.edu
Subject: [Histonet] CD34 Control
To: 'jreichensper...@siumed.edu' jreichensper...@siumed.edu
Cc: 'histonet@lists.utsouthwestern.edu'
histonet@lists.utsouthwestern.edu
Message-ID:
83f4d81747a7094dafe3ae87151ecb941ce4144...@sykechxvs01.nslijhs.net
Content-Type: text/plain; charset=us-ascii
Joel,
A section of skin will be a fine CD34 control. All collagenous
connective tissue, including the dermis, are rich in CD34+ dendritic
interstitial fibroblasts as well as CD34 positive endothelium in the
resident vessels. Actually, in my lab we use a section of fallopian tube
from tubal ligation, they are loaded with the fibroblasts and vessels.
Jeff Silverman
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