Hi Jean, We use Cell Marque's (via VMS) clone 24. We have to throw the entire arsenal at our disposal at these stains but they're acceptable.
Good luck Jean; any questions, you know where we live! Linda Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Taylor, Jean Sent: Thursday, August 19, 2010 9:45 AM To: '[email protected]'; '[email protected]' Subject: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) Hi Everyone, I'm trying to find out what company and clone most labs are using for PAX-5 (BSAP). Thanks! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of [email protected] Sent: Wednesday, August 18, 2010 12:00 PM To: [email protected] Subject: Histonet Digest, Vol 81, Issue 23 Send Histonet mailing list submissions to [email protected] To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to [email protected] You can reach the person managing the list at [email protected] When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: CD34 positive control (McMahon, Loralee A) 2. Ventana Renaissance Containers (ricky hachy) 3. CD34 Control (Silverman, Jeffrey) ---------------------------------------------------------------------- Message: 1 Date: Wed, 18 Aug 2010 12:17:39 -0400 From: "McMahon, Loralee A" <[email protected]> Subject: RE: [Histonet] CD34 positive control To: Joel Reichensperger <[email protected]>, Histonet <[email protected]> Message-ID: <c27aa2a01cef31469813089e226f582e02d5a7c...@urmcms7.urmc-sh.rochester.ed u> Content-Type: text/plain; charset="us-ascii" Just about any tissue should work. It stains for Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts We use a sausage style control here with about 30 different types of tissue. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: [email protected] [[email protected]] On Behalf Of Joel Reichensperger [[email protected]] Sent: Wednesday, August 18, 2010 12:05 PM To: Histonet Subject: [Histonet] CD34 positive control I have a doctor who wants to stain some tissue with cd34. I need to know if anyone can recommend a good positive control tissue for this antibody. The staining will be done in paraffin embedded sections. Thanks in advance. Joel -- Joel Reichensperger Researcher II Southern Illinois University Plastic Surgery Institute [email protected] 217-545-7309 (Office) 217-545-1824 (Fax) _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 18 Aug 2010 16:28:39 +0000 From: ricky hachy <[email protected]> Subject: [Histonet] Ventana Renaissance Containers To: Histonet <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset="iso-8859-1" Hello , I need 4 good plastic containers, where you fill with xilene,alcool....... for the VENTANA RENAISSANCE . Anyone could sell me ? Regards Ricky ------------------------------ Message: 3 Date: Wed, 18 Aug 2010 12:30:58 -0400 From: "Silverman, Jeffrey" <[email protected]> Subject: [Histonet] CD34 Control To: "'[email protected]'" <[email protected]> Cc: "'[email protected]'" <[email protected]> Message-ID: <83f4d81747a7094dafe3ae87151ecb941ce4144...@sykechxvs01.nslijhs.net> Content-Type: text/plain; charset="us-ascii" Joel, A section of skin will be a fine CD34 control. All collagenous connective tissue, including the dermis, are rich in CD34+ dendritic interstitial fibroblasts as well as CD34 positive endothelium in the resident vessels. Actually, in my lab we use a section of fallopian tube from tubal ligation, they are loaded with the fibroblasts and vessels. Jeff Silverman ------------------------------ _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 81, Issue 23 **************************************** _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
