It should be "In situ" and not 'In site'. Sorry for the typo error.
-Partha
On Sun, Oct 11, 2009 at 9:59 PM, Parthasarathy Sampathkumar <
spart...@gmail.com> wrote:
> Hi Jing,
>
> Methods to perform In site proteolysis are available in the following
> publications:
>
> http://www.plosone.org/arti
Hi Jing,
Methods to perform In site proteolysis are available in the following
publications:
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0005094
http://www.nature.com/nmeth/journal/v4/n12/abs/nmeth1118.html
Good Luck,
-Partha
On Sun, Oct 11, 2009 at 5:13 PM, jwangliang
Sometimes it is better that it takes time for crystals to appear.
Remember that crystallisation is a purification procedure. A way to
decrease the speed of crystallisation is to use Dunlop's and Haze's drop
dilution method (K. V. Dunlop & B. Hazes (2003). When less is more: a
more efficient vap
Hi folks,
Here's the reply I received from the LeonardTV team concerning: i)
broadcasting in English; ii) Proteopedia.
i) Hello, regrettably for your friend, at first we are going to have our
broadcasts only in French. It could be that one day we will have an
English version, if our technic
Hi everyone,
I am trying to use molprobity to check a structure in coot (platform is
64-bit vista). I followed the instructions found here to set up molprobity:
http://www.ysbl.york.ac.uk/~lohkamp/coot/wincoot-faq.html . From the logs,
it seems like reduce runs OK (it says that it "Added 6076 hy
Hello,
I am sorry to put this question which didn¹t related to the CCP4 software.
I couldn¹t get the crystals from the protein only. So I want to try the
different way to work it out. I hear that it is possible to set a crystal
tray of protein with Trypsin protease. I will be very appreciated abou
Hi James,
have you tried limited proteolysis on your protein and see if you can
identify a stable fragment. Then re-clone and re-crystallize your
protein. Or a very stupid suggestion, how does your size exclusion
peak look like ? What you're not running your protein over a SEC to
polish i
I've always found it is a much more effective and delightful use of my
time to mope sullenly in my office, communicate with my family members and
colleagues while restricting my working vocabulary to monosyllabic grunts,
or pensively pace the halls, inflicting my abject misery upon hapless
passers-
depending on the importance of the project, I would be tempted to just
wait for the crystals to grow, and in the meantime:
- work on another project
- start a new project
- write a grant application, a paper, a review...
or a combination of the above.
Mark
Quoting William Scott :
Dear James:
It will help more if we send it to James ;)
On Oct 11, 2009, at 9:15 AM, gauri misra wrote:
Dear James,
As there are indications of protein degradation that have been
suggested in
previous postings, i think adding some protease inhibitors right at
the
stage of purification may provide so
Dear James:
It is extremely hard to make generalizations, but if it takes months for
crystals to appear, the following questions are worth asking:
1. Is the actual concentration of macromolecule, precipitating agent, etc
required for crystallization higher than what you are using? A closed
vapor
Dear James
What size are your drops? If it takes that long for crystals to grow, it is
very likely that something happens to the protein that stopped nucleation
previously... it generally means some form of protein degradation. I would
start by investigating exactly what you have on the crystals. I
Dear crystallographers,
Sorry for the non-ccp4 query. I am new to this field and need some
suggestions. My question is, why some protein takes longer time to
crystallize, say 6-8 months, and it is the only condition to get the
crystals.? What are the ways to get the crystals faster.
The crystal a
The big problem here is that it doesn't recognise the NOANISOU
keyword. So I presume that you are picking up an old version of
CCP4, or more likely a Coot version of pdbcur. Mmmm the fact that
the banner doesn't report user or date or time suggests a dodgy
version.
I would have to defer to a Mac p
jpd,
I have seen this type of error message in situations where some memory
limit was exceeded.
Based on googling with 'common "relocation truncated to fit" ', the
memory limit has something to do with a COMMON block exceeding its max
size, see -
http://coding.derkeiler.com/Archive/Fortran/
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