[Histonet] 53BP1 and gamma-H2AX antibodies

Good morning Histonet, Does anyone know of 53BP1 and gamma-H2AX antibodies that work in FFPE rat tissue (lung, liver, spleen, brain). Would appreciate any suggestions anyone might have, Best wishes, Michele Dr Michele Ellender Biological Effects Department, Centre for

[Histonet] Question

In power outages ( bumps) does the Sakura cryostat resent micron settings? ( factory setting? ) I figure that this would be the fastest way to get an answer. Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438

[Histonet] Histology Jobs in NY

Dear Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career

[Histonet] Looking for someone with muscle biopsy experience in NY

Dear Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career

[Histonet] RE: 53BP1 and gamma-H2AX antibodies

Rabbit anti-Phospho-Histone H2A.X (Ser139) Antibody #2577 from Cell Signaling works very well in FFPE(dilution 1:100 in immunofluorescence). Don't know good anti 53BP1. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810

[Histonet] about histonet mailing list

could you pls delete my e-mail adress from mailing list thanks ozge -- *Mutlu Günler :) Özge KARADENİZ* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] CLIA classes?

Hi all,   Does anyone know of a class available for learning about CLIA compliance and regulations, specifically for a Mohs/Dermpath lab?   Thanks for any advice you can give me on this! Lyn L. Treeger, B.S.HT(ASCP) ___ Histonet mailing list

[Histonet] Her2 Fixation Requirement Responses/Thanks

Your information and suggestions helped me formulate a plan, so thank you to those who took the time and responded to my inquiry yesterday. Paula Lucas ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

FW: [Histonet] CLIA classes?

I work at at derm office and this would be great to know about if there was one. Please please please include me in the list of responses. Thanks! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sands, Jenn

Re: [Histonet] Trace Gas companies

Thanks to all who gave the info. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Katelin Lester histol...@medsurgpath.com 02/02/2011 08:11 PM To kim.dona...@bhcpns.org cc histonet@lists.utsouthwestern.edu

RE: [Histonet] slide labeler advice

Hi Liz, We use Leica's IPC and IPS equipment and have found a simple solution to using them with our LIS. If you connect a computer (CPU) only, to each IPC or IPS you want to interface, you can use these units like any other printer that your LIS lists. We routinely print slides and cassettes

[Histonet] Formaldehyde monitor

Anyone know of a cheap formaldehyde gas monitor or concentration test method? I found a couple of monitors on Google, but they seem to run abour $1500+ Bob Sepulveda VA Med Center Los Angeles ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Consult accessioning question

When accessioning a case for consultation, do you use the date on the paperwork from the referring hospital/pathologist as the new Date-of-service? If so, what do you do when the paperwork gives a date (say Friday) and then the specimen is not sent out until the following Monday? Thank you.

Re: [Histonet] Consult accessioning question

Richard, We use the date of accessioning as the DOS and we wouldn't accession it until we have received the specimen/materials. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195

RE: [Histonet] Formaldehyde monitor

Lab Safety Supply has the option to order the badges for formaldehyde and Xylene and when you order, you pre-pay for the analysis. Formalin is $476.00 for 5 monitors. Xylene is $259.00 for 4 monitors. They email you a very nice report for your file, and it only takes about a week to 10 days.

Re: [Histonet] Formaldehyde monitor

Call Advanced Chemical Sensors Inc @ 561-338-3116 I think they are like $100 each. Last I looked anyway. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Bob Nienhuis bob.nienh...@gmail.com Sent by:

RE: [Histonet] Consult accessioning question

Same for us. You need both DOS from office as well as date received. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of wanda.sm...@hcahealthcare.com Sent: Thursday, February 03, 2011 1:06 PM To:

Re: [Histonet] Off Topic: researchers very funny

yes our lab received it last week! And yes we've been posting it to Facebook as well (as someone else on Histonet commented!) If the lady in the video never gets her PhD, she may want to consider the entertainment industry! --On Tuesday, February 01, 2011 10:29 PM -0500 Kimberly Tuttle

RE: [Histonet] Consult accessioning question

For Medicare, my understanding would be to use the DOS if less than 30 days, and to use the date pulled from archives if more than 30 days. At least that is the regs for additional tests. I think it would be the same for consult as well. Joyce Weems Pathology Manager Saint Joseph's Hospital

[Histonet] TTF-1/background staining

How can I eliminate the background or as you say, non-specific staining? I'm no expert here, I admit, and so I'm asking for your help and suggestions. I have searched the Histonet archives, and a lot of what I'm seeing deals with animal or rodent tissues, and a lot of it was confusing to me.

[Histonet] Microwave processing

Does anyone know what the compliance is, if any, with JACHO or CAP regarding the pathologist doing a paper QC on each microwave run? We have been having them sign a QC sheet that we hand in with each microwave run and our looking at ways to rid our area of some of the unnecessary paperwork!

FW: [Histonet] Formaldehyde monitor

We use Advanced Chemical Sensors. Acsbadge.com is website. I also used to use Surgipath. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Nienhuis Sent: Thursday, February 03, 2011 11:29 AM To:

Re: [Histonet] Microwave processing

Dorothy: You should follow for MW processing the same procedure you follow for any traditional tissue processing. What you have to document is that when you started to use the MW processing there was a documented QC to compare the results between the method you used and the new with the MW

[Histonet] Temporary histology position in San Diego Ca.

GNF is currently seeking a temporary Scientific Associate to join the Histology group, for the beginning of March 2011 through the end of July 2011. Perform necropsies, tissue processing, slide sectioning, routine staining, special staining, immunohistochemical staining and complex procedures

RE: [Histonet] TTF-1/background staining

Hi Paula, I am probably reading between the lines here but on your TTF protocol do you use a streptavidin biotin detection system and if so do you block for endogenous biotin, liver will have endogenous biotin where lung may not have as much? thanks Debbie Siena HT(ASCP)QIHC Technical

RE: [Histonet] Her2 Fixation Requirement

Does anyone have issues with inking the specimen after it has been placed in formalin? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Wednesday, February 02, 2011 12:59 PM To: Paula

[Histonet] Karnovsky and Roots stain

I am looking into a project involving motor end plate staining. Literature that I've found continually references Karnovsky and Roots from the 60s. However the papers are not supplying all the details. Does anyone do AchE staining by this method on fresh frozen, unfixed tissue sections? If

RE: [Histonet] TTF-1/background staining

Hi Paula I do not believe that RTU Abs really exist. No company can produce an antibody that is suitable for use by all laboratories taking in to consideration how tissue can be handled differently from lab to lab. I have used many RTU Abs which I have had to dilute to get optimal staining

Re: [Histonet] TTF-1/background staining

Hi Paula, You mentioned the staining in a liver specimen using the cell marque antibody against TTF-1. The clone named 8G7G3/1 that cell marque sells is known to stain liver cells and liver cancer. Your pathologist might be interested in knowing that. Here is a reference you can give him: