Re: [Histonet] Pas Digestion

As Jennifer mentioned, enzymes have optimal temperatures at which to work.  But also optimal pH.  Is your 0.5% amylase in water or a correct buffer? Ray, Seattle, WA - Original Message - From: "Loree via Histonet Lager" To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, Novembe

Re: [Histonet] Pas Digestion

The optimal temperature for most enzymes in 37 degrees Celsius. From: "Lager, Loree via Histonet" To: "'histonet@lists.utsouthwestern.edu'" Date: 11/03/2015 12:38 PM Subject:[Histonet] Pas Digestion Hello, This is my first time using the histonet, as our veteran user ret

[Histonet] Paraffin sectioning for DNA analysis

Hello Listers, Which techniques are you all using to avoid cross contamination when cutting multiple sections from multiple blocks for DNA analysis? Way back I used to use DEP-C treated water, wore gloves, changed the blade between blocks and wiped down the surface of the knife holder plate w

[Histonet] Pas Digestion

Hello, This is my first time using the histonet, as our veteran user retired. We've been having an intermittent problem with incomplete glycogen digestion on PASD. Our digestion solution is 0.5% α-Amylase, Type VI-B from porcine pancreas (Sigma), which we freeze in small aliquots and thaw for

[Histonet] LFABP and PHH3 Abs

Dear Experts, We are trying to optimize LFABP( Liver marker) and PHH3( Mitosis marker). Anyone willing to share their protocol or suggestions that can help expedite the process is much appericiated. We have both Leica & Ventana instruments. thank you in advance! Kiran

Re: [Histonet] CD8 IHC on FFPE mouse tissue -what your experience?

I am interested to see what you hear BrettI need to do these same markers and am not having good luck in FFPEfrozens , great...FFPE NOT! C On Tue, Nov 3, 2015 at 7:32 AM, Connolly, Brett M via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi all, > I have a project that will be

[Histonet] Acid hematin

I saw a Histonet post that Peggy Wenk had made concerning the recipe for alcoholic formalin for processing. She noted that you use the solution unbuffered.  How do you control acid hematin pigment in such a solution? We use a commercial alcoholic formalin “concentrate” that is diluted with alc

[Histonet] FW: A blogpost for parents, or the children of parents.--problem with link??

If the previous link did not work in your browser, try the full address http://www.chicagonow.com/downsize-maybe/2015/11/if-you-are-a-parent-or-have-ever-had-one-the-forever-plan-is-for-you/ Read, comment, subscribe Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterpris

[Histonet] (no subject)

How many of you have a cytology dept where your cyto techs do all their own prep and reports? If you don't, do you have a cyto prep aide or does a histo tech help? Also, how many of you have histo and cyto as the same department with an AP manager? Does the AP manager oversee transcription as

[Histonet] A blogpost for parents, or the children of parents.

Hello Netters--- Something to bring a smile to your "leisure" time. Click here for blog Read this one, because we have all been there! Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-020

[Histonet] Symphony users

Symphony users, can you message me?  I want to talk about prices, what you like/dislike about it off line.  Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] CD8 IHC on FFPE mouse tissue -what your experience?

Hi all, I have a project that will be looking at CD8 expression in some FFPE murine tissue. Past discussions here have mentioned the 14-0808 CD8a antibody from eBiosicence. For those of you have had the opportunity to use this antibody I would like to hear your experiences/ recommendations abo