[Histonet] cadaver tissue processing for histology

Hi, I got some cadaver tissue floating in "LTP embalming fluid w/ phenol". Can I put those straight into the processor? Or has to be rinsed/soaked before? If so what should I use? My Internet searches did not bring up anything useful. Thank you! Hanna Preiszner ETSU/QCOM Dept of Pathology __

Re: [Histonet] special Stains under a hood

I have never (3 different places, 30+ years) heard of requiring special stains to be performed under a hood. Certainly, there are a few reagents that might need benefit from a hood (concentrated Ammonium Hydroxide) but generally, they are performed on an open counter next to a sink. If you are

[Histonet] Alcian Blue digested w Hyaluronidase - any alternatives?

Help?   we have a student with one of the online schools and we're doing specials like it is 1972.  She has to do a Al Blue 2.5 w Hyaluronidase digestion.   We haven't done this since the development of clinical IHC two dozen years ago - Is there any other way to acheive this outcome on a colon

[Histonet] Special stains and hoods

25+ years in Histology, half the places I worked had vents on the wall near the countertop where we did specials, otherwise it was on the counterclosest to the sink. The only times we were time doing anything under a hood was for frozen section staining because of the fresh tissue, mixing sulfer

Re: [Histonet] Special stains and hoods

I guess the question is, who told you this and what is their reference? Most special stains don't require a hood. Only those that have volatile chemicals or odors that may be offensive (ie, ammonium sulfide). We have some areas we do specials without a hood. Our main special stain areas have a

Re: [Histonet] Special stains and hoods

That is certainly an odd request… I have never performed any special stains under a hood any place I have ever worked. Always done on a counter top near a sink… Is someone in your department extremely sensitive to odors? Or have asthma? We have a tech who experiences attacks if there are certain ch

[Histonet] MSH2 and MSH6 Loss of Expression

Fellow Histonet Members - We are in the process of moving our IHC bench from the Leica BOND to the ROCHE ULTRA and are in the mist of antibody validations. We are having great difficulty in finding negatives to validate for MSH2 and MSH6. Would anyone have any loss of expression MSH2 and MSH6 ap