Hi Clay,
The first solution is methanol.
So lab grade methanol will do.
The blue colour is from an innocuous dye added to differentiate from water (and
ethanol used in cytology fixation). This helps us poor cytologists when we are
doing ROSE at FNAs.
Regards
Tony Henwood JP, MSc, BAppSc,
We (CPQA) ran our first EQA to included a review of tissue processing and
staining of various tissue and the report might be of interest to you and
others. Contact me directly if you, or anybody else, would like a copy of the
report.
John
Sent from ProtonMail for iOS
On Tue, Oct 5, 2021 at
Hi,
I’m looking for some advice on using LR white on an osmium stained tissue.
Unfortunately, I don’t have access to an ultra-microtome or diamond knives.
Has someone here tried sectioning LR white with tungsten carbide knives on
a motorized rotary microtome? Are there any sectioning artifacts
If you are looking to prioritize certain tissues slides to the pathologist,
I think separate small biopsy runs are a great idea. You can process them
with a much shorter run cycle.(90-120 min). You can actually do several
runs per day. This is a good way of decreasing your TAT for all blocks and
From: Hannen, Valerie
Sent: Monday, October 04, 2021 2:54 PM
To: 'histo...@list.utsouthwestern.edu'
Subject: FW: Biopsy processing
From: Hannen, Valerie
Sent: Monday, October 4, 2021 11:03 AM
To: 'histo...@list.utsouthwestern.edu'
Subject: Biopsy processing
Good Morning All... My
Hello,
We are in the market for a new/refurbished microtome. We currently have a
Micron HM355s, and Micron HM 335E. We are interested in buying something
comparable. Any suggestions? We are looking at Micron and Leica. Good or bad
reviews would be appreciated.
Thanks,
Silvia Bonner,
Hi Guys,
We normally buy human tissue blocks to use them as controlsor to develop IF
assays in our laboratory. However, most of these blocksappeared to be fixed or
processed adequately. I am wondering if anybody has amethod to assess the
quality of these tissue blocks besides H stain.
