Aren't they going to offend the mail histotechs with that name? Just kidding.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
39037 N 11th Ave
Phoenix, AZ 85086
C 720-281-5406
prueg...@hotmail.com
Doug Ruegg
C720-281-5407
douglas...@hotmail.com
From:
this is a strange post on histonet
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
39037 N 11th Ave
Phoenix, AZ 85086
C 720-281-5406
prueg...@hotmail.com
Doug Ruegg
C720-281-5407
douglas...@hotmail.com
From: histonet-requ...@lists.utsouthwestern.edu
Sent:
I used tonsil as + control.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
39037 N 11th Ave
Phoenix, AZ 85086
C 720-281-5406
prueg...@hotmail.com
Doug Ruegg
C720-281-5407
douglas...@hotmail.com
From: histonet-requ...@lists.utsouthwestern.edu
Sent: Friday, June
I would suggest using something like histogel or agar to make layers of blood
drops in a tube, then freeze them and make frozen sections. Sounds tricky.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
39037 N 11th Ave
Phoenix, AZ 85086
C 720-281-5406
prueg...@hotmail.com
Doug Ruegg
C720-281-5407
The blue stain from prussian blue is a chemical reaction and indicates that
there is a lot of iron in the section, you would not want it not to stain some
of the iron there would you? I agree with John, take an adjacent section for a
general stain.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC
This is all good advise. I always picked up section and tapped the slide on
counter and then dried standing up. This is really a problem if you are at all
above 5K feet in altitude where water boils violently at 92-93dc, which will
destroy your tissue section. I have written a couple of papers
I have done it, but you are right, I had my own private business, not sure why
it would be a problem, especially for research.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
I agree with this point and as far as clocking in and out, I would think you
could work out something like getting paid piece mill, perhaps charge per slide
or block cut, that way you could do it on your own time and not have to clock
in.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E
From: John Garratt
Sent: Thursday, January 23, 2020 10:51 AM
To: Terri Braud
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Need a procedure
Hi Terri, I suggest you use Histogel for block preparation. It works
exceptionally well, it is good
I never minded being called in to assist for an aspect of transplants at the U,
I figured I was helping a very needy patient.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
Terri, when I was in animal research we used the blood from the animal to make
plasma and combined it with some thrombin from the hospital pharmacy. Of
course that is tedious especially since histogel is available. I warmed the
histogel by placing a tube of it in a beaker of hot water, do not
whether I used an ice pack or not, I always bagged each block in it's own
individual container/plastic bag, etc., just in case there was melting the
tissue could possibly be recovered.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C
here here
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
From: Joseph Saby
Sent: Tuesday, March 5, 2019 9:47 AM
To: Jordan, Kelley; Mark Tarango
Cc: HistoNet
Subject: Re:
Maybe that is the trouble, they keep passing these technical issues on to
Marketing instead of R
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
___
Histonet
In my opinion this issue is related to the fact that HT's are not required to
have BS degrees, like Med Techs and Cytotechs, and that clia/cap does not
require ASCP certification to work in a histology lab. We have always been the
red headed step child in the lab because of this. This is why
Stefano, after cutting the sections, dry them on a heat plate, then they can be
stored dried stacked next to each other.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
good advise Carl and you asked some of the things I was wondering.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
From: Hobbs, Carl
Sent: Saturday, February 2, 2019 12:38
Especially a very blood tissue like GP spleen.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
From: Patsy Ruegg
Sent: Thursday, January 31, 2019 11:51 AM
To: Jan Shivers;
In my experience it is not that GP have a higher peroxidase level, it is frozen
sections in general that cannot be blocked with h202, unless they are fixed for
a long time in formalin. What are others experiences with h202 blocking on
frozen sections. I always used an IHC detection system
Hi Richard,
How are you? Dianova is a really good ab supplier, I used their CD31 for
animal research and it was excellent. Happy Holidays. Patsy
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
From: ancillaryp...@mac.com
Sent: Saturday, October 7, 2017 6:03 PM
To: ihcrg ihcrg
Subject:
I have seen so many more problems with auto coverslippers, they break down, the
cover does not hold up over time for archiving, etc., that I would would
probably chose an autostainer.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C
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