[Histonet] AedestaT-cell/tissue preservation (ACTP) media for muscle bx's

information I would appreciate it. It would be great to be able to restore these muscles when needed. Thank you, Sharon Allen Senior Medical Technologist Neuropathology Lab-MS435U Health Sciences Centre 820 Sherbrook Street Winnipeg,MB, CA R3A 1R9 e-mail: sal...@dsmanitoba.ca This email

[Histonet] Phosphorylase method

to compare other methods without PVP. Also, does anyone know the purpose of PVP, glyceron or dextrin in this test? Thanks for any help, Sharon Allen Senior Medical Technologist Neuropathology Lab-MS435U Health Sciences Centre 820 Sherbrook Street Winnipeg,MB, CA R3A 1R9 e-mail: sal

RE: [Histonet] Phosphorylase method

not be necessary. Thanks again for your interest; I would appreciate your input. Have a good day, Sharon Allen Senior Medical Technologist Neuropathology Lab-MS435U Health Sciences Centre 820 Sherbrook Street Winnipeg,MB, CA R3A 1R9 e-mail: sal...@dsmanitoba.ca -Original Message- From: Mitchell

[Histonet] CAP approved shelf life of dyes solutions

. Thanks Sharon Allen Senior Medical Technologist Neuropathology Lab-MS435U Health Sciences Centre 820 Sherbrook Street Winnipeg,MB, CA R3A 1R9 e-mail: sal...@dsmanitoba.ca This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally

FW: Subject: Re: [Histonet] Storage of Frozen Tissues

Hi, We freeze store muscle bx's brain tissue at -70°C since the neuro lab was started (around 1980). We started using cryogenic containers after we found that over time the tissue would dry out with any other type of storage we have tried them all. Hope this helps, Sharon Allen Senior

[Histonet] FW: standard for sm bx microtomy

Hi, Is there is a standard used for small biopsy Microtomy? I am wondering how other sites cut their biopsies (ie the number of sections per slide and the amount of roughing in between sections)? I am also curious about the number of slides per biopsy. Thanks for your help, Sharon Allen

[Histonet] FW: Quality Modules

Hi, I am looking for information on Quality Modules that can be added into an existing IT system and system with great Quality Modules built into their IT platform. Thanks for any information. Sharon Allen Senior Medical Technologist Neuropathology Lab-MS435U Health Sciences Centre 820

[Histonet] FW: Quality Modules

I am looking for information on Quality Modules that can be added into an existing IT system and system with great Quality Modules built into their IT platform. Thanks Sharon Allen Senior Medical Technologist Neuropathology Lab-MS435U Health Sciences Centre 820 Sherbrook Street Winnipeg

[Histonet] FW: standard for sm bx microtomy

Hi, Is there is a standard used for small biopsy Microtomy? I am wondering how other sites cut their biopsies (ie the number of sections per slide and the amount of roughing in between sections)? I am also curious about the number of slides per biopsy. Thanks for your help, Sharon Allen

[Histonet] Nile Red stain on Froz. muscle bx's

Hi, Does anyone have a method for staining frozen skeletal muscle bx's with Nile Red /or Nile Blue for the detection of lipid droplets. Our Neuropathologist saw the stain at a conference would like us to try it. Thanks Sharon Allen Senior Technologist Neuropathology Lab MS435U Health Sciences

[Histonet] SV-40 control blocks or slides

Hi, I am looking for a lab or a supplier for SV-40 control blocks or slides. If anyone can help us out it would be appreciated. Thanks Sharon Allen Senior Technologist Neuropathology Lab MS435U Health Sciences Centre 825 Sherbrook St. Winnipeg, Manitoba R3A 1R9 Ph# 787-4615 This email

[Histonet] FW: goat anti-rabbit FITC

respond to: dwat...@hsc.mb.ca or to me I can pass it along. Thanks for any help you can provide. Sharon Allen HSC, Wpg, MB, Ca This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any

[Histonet] Need Prion antibody Clone 3F4

would be very much appreciated. Sharon Allen Neuropath Lab sal...@hsc.mb.ca This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying

[Histonet] good Calcafluor Auramine/Rhodamine staining method for FFPE tissue

Hi, I am posting this question for Lisa, Our Microbiology department would like to find a good calcafluor staining method and a good Auramine/Rhodamine staining method for FFPE tissues. Any suggestions? Would you be able to post this for me? Thanks, Lisa Lisa Manning MLT, BSc. Pathology

[Histonet] Bielschowsky/Luxol Fast Blue stain

Hi, Does anyone have a method for doing a combination of Bielschowsky Luxol Fast Blue? If anyone has the method any comments on it I would appreciate it. Thanks Sharon Allen HSC, Winnipeg, MB, CA sal...@hsc.mb.ca This email and/or any documents in this transmission is intended for the addressee

[Histonet] Book specific for Neuropathology techniques/methods

Hi, Can anyone on the Histonet recommend a good book of Neuropathology techniques/methods for use in a Neuropathology Lab. Need more up to date reference material. Thanks Sharon Allen Neuropathology Lab HSC - Wpg sal...@hsc.mb.ca This email and/or any documents in this transmission

[Histonet] Amyloid staining on frozen muscle bx's

Hi, What stain works for amyloid on frozen muscle bx tissue? I did the a Congo Red stain without much success. Thanks Sharon sal...@hsc.mb.ca This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information.

[Histonet] Artifact in Muscle bx's frozen for extended time

in if you do not get this artefact? Suggestions for long term storage would be very much appreciated. Thanks Sharon Allen Senior Neuropathology Technologist HSC WPG, MB, CA This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged

[Histonet] RE: Prion Contamination

Hi, I was reading your email of Jan. 15/09 on the subject of Prion Contamination. In it you had made the following statement, which I was curious as to where this information had come from. the procedure to inactivate using formic acid is followed before fixation and processing. If the tissue is

[Histonet] Modified SDH

muscle an abnormal muscle stained with this method it would be very helpful. Thanks for any help, Sharon Allen Neuropathology Lab HSC - Winnipeg, MB CA [EMAIL PROTECTED] This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged

[Histonet] NSE histochemical stain

of Pathology. But am not getting a strong reaction. Having never seen a positive slide I nor the Neuropathologist know for sure what we are to be seeing. Does anyone have a picture of a slide they would be willing to share with us. Thanks in advance for any help you can give me. Sharon Allen