[Histonet] ISH decal specimens
Hello all, Recently, the Kappa/Lambda ISH has quit working on our decal specimens. When we first validated ISH, we tested decal specimens that stained appropriately. We use the DECAL Stat from StatLab. I did NOT do the initial ISH validation back in 2019, but as far as I know, we have not changed which Decal solution we use since then. Has anyone else experienced this problem? I realize the other option is to run K/L IHC on the decal specimens, but we discontinued those after we brought the ISH on board. My medical director wants me to try to isolate the problem. My troubleshooting has only revealed that the decal is the common denominator. Thanks for any help! Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Peroxidase stain on peripheral smears
Hello, I am seeking some guidance in regards to a peroxidase stain. We have always used the Kaplow Method to stain peripheral blood smears, and up until recently, we have had no issues with getting it to work. However, no matter what we try in recent months, we have not been able to get it to work. Several different techs have tried it and we have ordered new reagents, but there are no granules to be seen Does anyone have any experience with peroxidase kits? I can see that Sigma-Aldrich has several available but I'm not sure what to go with Any and all help would be greatly appreciated! Thank you, Kendra Beechie MLS/HTL (ASCP), Technical Consultant CHI St. Alexius Health Bismarck, ND Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet