[ccp4bb] postdoc positions in TIME-RESOLVED macromolecular crystallography
Dear Colleagues, two post-doc positions are immediately available at the University of Wisconsin-Milwaukee in the departments of physics and chemistry/biochemistry. Please forward this text to interested candidates. With best regards Marius Schmidt Position 1 A postdoctoral position is immediately open at the University of Wisconsin-Milwaukee (UWM)/USA in the field of time-resolved macromolecular crystallography, initially for a period of one year, with the option to extend for a second year. The candidate will work with Marius Schmidt, department of physics, and Andy Pacheco, department of chemistry and biochemistry. The main duties will include crystallization of non-heme and heme iron proteins, collection of monochromatic and polychromatic X-ray data (in house and at the nearby Advanced Photon Source of Argonne National Laboratory), and helping to train graduate students in protein crystallographic techniques. Experience with protein handling is required; skills in computer programming are definitely a plus. Training in time-resolved crystallographic techniques will be provided. This is a great opportunity to work in an interdisciplinary environment, at the interface of biology, chemistry and physics. Candidates with a background in the relevant areas are invited to send an application to either Andy Pacheco (apach...@uwm.edu) or Marius Schmidt (m-schm...@uwm.edu) from whom further information can also be obtained. Please include in the application a CV, a summary of research experience and interests, and contact details for three referees. Position 2 A postdoctoral position is immediately open at the University of Wisconsin-Milwaukee (UWM)/USA for a synthetic organic or inorganic chemist with experience in photochemistry, initially for a period of one year, with the option to extend for a second year. The candidate will work with Marius Schmidt, department of physics, and Andy Pacheco, department of chemistry and biochemistry. The focus of the project will be to synthesize and characterize novel photoactive compounds for use in time-resolved macromolecular crystallography. Experience with laser photochemistry is desirable but not required. This is a great opportunity to work in an interdisciplinary environment, at the interface of biology, chemistry and physics. Candidates with a background in the relevant areas are invited to send an application to either Andy Pacheco (apach...@uwm.edu) or Marius Schmidt (m-schm...@uwm.edu) from whom further information can also be obtained. Please include in the application a CV, a summary of research experience and interests, and contact details for three referees. Dr.habil. Marius Schmidt Asst. Professor University of Wisconsin-Milwaukee Department of Physics Room 454 1900 E. Kenwood Blvd. Milwaukee, WI 53211, U.S.A. phone: +1-414-229-4338 email: m-schm...@uwm.edu http://users.physik.tu-muenchen.de/marius/
[ccp4bb] Ad rerun: Postdoc in PX at SGC (U Oxford)
Hi, Take Two of this ad -- hit and if annoyed :) I am recruiting for a postdoc position in my group, available immediately. This is the Protein Crystallography group of the Structural Genomics Consortium, Oxford. In particular, hard-core crystallographers strong on theory and (optionally) with a bent for programming, are invited to apply. For details, please see: http://www.sgc.ox.ac.uk/jobs/H909013.html, or contact me for further info. (If you miss the deadline, don't despair, send your application anyway, directly to me.) The remit of the Structural Genomics Consortium (SGC) is to solve human proteins of medical relevance and place them in the public domain without restrictions; it is funded by a consortium of public and industrial funders, and consists of independently operating departments in the Universities of Oxford and Toronto, and Karolinska Institutet (Stockholm). The Oxford site has solved almost 300 such structures in the last five years, and is now halfway through Phase II which is funded till June 2011; this phase has an increased emphasis on chemical biology and membrane proteins. (http://www.sgc.ox.ac.uk) The Protein Crystallography group collaborates tightly with the 5 other groups to get their purified proteins crystallized and solved (five per month). Additionally, our research revolves around methods development, for which we're ideally positioned thanks to accumulated historic data, extensive automation equipment, close links to vendors, and especially, access to the many proteins of high biological relevance. The current emphasis is on methodology for rapidly and systematically generating co-crystal structures, by expanding on the high-throughput approaches that underpin our success with novel targets; this is of high relevance to the the SGC's exploration of open source chemical biology. Frank -- Dr Frank von Delft Principal Investigator: Protein Crystallography Structural Genomics Consortium Oxford University +44 1865 617583
Re: [ccp4bb] install issue on win vista - where is the ccp4i executable? RESOLVED
It looks like there is a problem with the single-user installer file. My previous install attemps had been using the single-user installer, launched from my vista user account. When I ran the all-user installer from my user account (in both cases I of course had to enter the adminstrator password to continue installation), after the mandatory reboot CCP4 so far looks okay. I do not know yet if everything is working, but at least now CCP4i launches. There is still no entry in the start menu, so that still needs to be fixed. Hardly a major issue, but it might have a similar cause to why the single-user installer does not seem to work. Thanks to Dima Klenchin for suggestions and analysis of the error messages I got from the single-user install, Stuart Endo-Streeter On Monday 27 July 2009 20:00, you wrote: > >I get the following error when trying to run ccp4i.tcl with bltwish.exe: > > > >Can't read "env(CCP4I_TOP)": no such variable while executing > >"file join $env(CCP4I_TOP) bin $system(OPSYS) startup.tcl" invoked from > >within > >"source [file join $env(CCP4I_TOP) bin $system(OPSYS) startup.tcl]" > > (file "C:\CCP4-Packages\ccp4-6.1.1\ccp4i\bin\ccp4i.tcl" line 20) > > OK, seems to me that your installation is totally hosed. You are > missing a whole bunch of environment variables. Either they were > never set "(did you reboot?) or they are pointing to the wrong > directories. > > I had no issues at all installing on XP. On Win2K, however, all > the pointers to Tcl had wrong directory and outdated executable > name. Had to fix it all manually. Maybe on Vista the installer > does not do what it's meant to do on tested XP... > > Dima -- __ Stuart T. Endo-Streeter Structural Biology and Biophysics Dept. Biochemistry LSRC C266 Duke University 919-681-1668 stuart.endostree...@duke.edu
Re: [ccp4bb] advice on laptop for crystallography
We have been using IBM (now Lenovo) T43P laptops for several years with great success and minimal issues (knock on wood). We are able to run everything (including HKL2000) with no problems on these machines. I am sure that the newer T60 series would behave similarly. Good luck - Steve -Original Message- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Salgado, Paula Sent: Tuesday, July 28, 2009 11:05 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] advice on laptop for crystallography Dear all I'm planning to upgrade my laptop and would like to run most crystallography programs. I was wondering if people could comment on their current prefered options. I would prefer not to go for a Mac, so any other alternative suggestions would be more than welcome. Also, I did a little research and it seems few laptops could deal with the resolution requirements for HKL2000 - any thoughts on that as well? Thanks in advance for all the help. Paula = Dr Paula Salgado Division of Molecular Biosciences Department of Life Sciences Faculty of Natural Sciences Biochemistry Building, 5th Floor Imperial College London South Kensington Campus SW7 2AZ London Tel: 02075945464 Fax: +44 (0)20 7594 3057 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system.
Re: [ccp4bb] advice on laptop for crystallography
Paula, I would also suggest upgrading your processor to one that is capable of hardware virtualization. If you plan on implementing any virtual machines to use multiple operating systems on your laptop, this upgrade may be worth it for the extra performance you gain. I can't comment on how much of a difference it actually makes for a cost-benefit comparison, but something to consider. -Andy > Dear all > > I'm planning to upgrade my laptop and would like to run most > crystallography programs. I was wondering if people could comment on their > current prefered options. I would prefer not to go for a Mac, so any other > alternative suggestions would be more than welcome. > Also, I did a little research and it seems few laptops could deal with the > resolution requirements for HKL2000 - any thoughts on that as well? > > Thanks in advance for all the help. > > Paula > > = > > Dr Paula Salgado > > Division of Molecular Biosciences > Department of Life Sciences > Faculty of Natural Sciences > Biochemistry Building, 5th Floor > Imperial College London > South Kensington Campus > SW7 2AZ > London > > Tel: 02075945464 > Fax: +44 (0)20 7594 3057
[ccp4bb] Senior Research Scientist position, University 0f Wisconsin-Milwaukee
THE UNIVERSITY OF WISCONSIN - MILWAUKEE Senior Research Scientist (equivalent to Research Assistant Professor) The Department of Physics at the University of Wisconsin - Milwaukee seeks outstanding applicants for a senior scientist position (equivalent to research assistant professor). Candidates must have: a Ph.D. in physics or closely related field, and at least two years of postdoctoral research experience in one or more of the following fields: theory of electron or X-ray scattering, advanced imaging algorithms, and/or numerical methods. Exceptional candidates from related fields will also be considered. A strong record of independent research evidenced by publications is required. Responsibilities include research in structure determination by scattering methods, development of advanced noise-robust algorithms for structure recovery and reconstruction, and leading research associates. Applicants should submit their resume (curriculum vitae), summary of relevant research experience, publications and patents lists, and names of three referees. To apply, please see www.jobs.uwm.edu/applicants/Central?quickFind=51047 . Review of applications will begin on September 1, 2009 and continue until the position is filled. As an equal opportunity/affirmative action employer, the University of Wisconsin - Milwaukee promotes excellence through diversity and encourages all qualified individuals to apply. For further information contact: Professor Abbas Ourmazd, Department of Physics, ourm...@uwm.edu University of Wisconsin-Milwaukee, P.O. Box 413, Milwaukee, WI 53201. -- Scanned by iCritical.
Re: [ccp4bb] Axygen MS-100 plate sealer: user comments?
Hi, I used the Axygen sealer for more than a year, and it worked fine. A couple of caveats - The sealer is cheaper than many automated sealers, but the seals are pre-cut, and are more expensive. If you are sloppy laying the seal over the plate it is relatively easy to not seal one edge (or two edges) of the plate. We never made the mistake of putting in a seal the wrong way up, but I imagine that would be a really tedious recovery process (scraping melted plastic seal off the heating block). What are the chances that a casual user will get a seal the wrong way up in your lab? We had to put strips of foil on the seal (ie, between the heating block and the seal) to ensure the centre of the seal sealed as well as the edges. The Axygen paper-backed seals worked really well, but weren't good for plates destined for the cold room - the paper backing absorbs moisture. cheers, Janet From: CCP4 bulletin board [ccp...@jiscmail.ac.uk] On Behalf Of Chris Lemke [christopher.le...@boehringer-ingelheim.com] Sent: 29 July 2009 05:50 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Axygen MS-100 plate sealer: user comments? Greetings all - I'm wondering if anybody out there can comment on the Axygen MS-100 MiniSealer's performance in pressure sealing crystallization plates and/or in heat sealing deep well blocks. Any user input would be greatly appreciated. Thanks in advance, Chris.
