Re: [ccp4bb] structural homologs as cross seeds
I know of at least one published example where seeding has been used to crystallise a number of homologues: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2243101/ (Sanchez-Weatherby 2006, Acta Cryst F 62:518) There are probably others out there... Certainly worth giving it a go. Best wishes, - Allister On 27 December 2013 19:29, Mahesh Lingaraju wrote: > Dear all, > > I was wondering if it sounds logical to use the crystals from a possible > structural homolog as seeds to induce nucleation ? (in terms of overall > sequence, the proteins are considerably different but based on sequence > alignment and structures from other related proteins, it is highly likely > the protein would have the same structure.) > > Please comment if any of you had experience with this. > > Thank you > > Happy holidays :) > > Mahesh >
Re: [ccp4bb] structural homologs as cross seeds
the differences are likely to be on the protein surface, and these would make the crystal contacts, i.e. it would be unlikely the protein could crystallise in the same crystal habit. Never say never in crystallisation (i.e. try anything), but I would go for other things first like additives, different crystallisation techniques, temperatures, concentrations etc. On 27 Dec 2013, at 20:29, Mahesh Lingaraju wrote: > Dear all, > > I was wondering if it sounds logical to use the crystals from a possible > structural homolog as seeds to induce nucleation ? (in terms of overall > sequence, the proteins are considerably different but based on sequence > alignment and structures from other related proteins, it is highly likely the > protein would have the same structure.) > > Please comment if any of you had experience with this. > > Thank you > > Happy holidays :) > > Mahesh
Re: [ccp4bb] structural homologs as cross seeds
I do not have experience with this but it is worth trying. On Fri, Dec 27, 2013 at 2:29 PM, Mahesh Lingaraju wrote: > Dear all, > > I was wondering if it sounds logical to use the crystals from a possible > structural homolog as seeds to induce nucleation ? (in terms of overall > sequence, the proteins are considerably different but based on sequence > alignment and structures from other related proteins, it is highly likely > the protein would have the same structure.) > > Please comment if any of you had experience with this. > > Thank you > > Happy holidays :) > > Mahesh >
[ccp4bb] structural homologs as cross seeds
Dear all, I was wondering if it sounds logical to use the crystals from a possible structural homolog as seeds to induce nucleation ? (in terms of overall sequence, the proteins are considerably different but based on sequence alignment and structures from other related proteins, it is highly likely the protein would have the same structure.) Please comment if any of you had experience with this. Thank you Happy holidays :) Mahesh
