[ccp4bb] Post-doctoral position at Umeå University, Sweden
Dear all, A post-doctoral position is available in the group of Dr. Ronnie Berntsson at the Department of Medical Biochemistry and Biophysics, Umeå University, Sweden. The project will concern structural and functional characterisation of the adhesion proteins involved in type 4 secretions systems from gram-positive bacteria. The candidate should have a genuine interest in a wide range of structural biology and biophysical/biochemical methods, and a proven track-record in molecular biology, protein expression and purification in bacterial systems. Experience in protein crystallography, electron microscopy and/or ITC is advantageous, but not essential. Depending on the successful candidates expertise and interest, the focus of the project could be varied towards more structural biology (mainly x-ray crystallography) or more biochemical/biophysical characterisation. Umeå University in Sweden has excellent facilities for molecular biology and protein research, including on site protein production facilities, a modern X-ray home source, state of the art electron microscopy and biochemical/biophysical characterisation facilities. The Berntsson lab is a newly established research group, studying different aspects of Type 4 Secretion Systems, with a focus on structural biology techniques. More information can be found at: http://www.medchem.umu.se/english/research/principal-investigators/berntsson-ronnie/ Funding is through the Kempe Foundation as a post-doctoral stipend, and is available for 2 years. The starting date is negotiable, but should preferably be during this summer. The position is open for both international and Swedish citizens. Informal inquiries and applications should be sent (with subject header set to “postdoc position”) by e-mail to ronnie.bernts...@medchem.umu.se. Applications should include a cover letter, CV, a list of publications and contact information for 2-3 referees. The application deadline is 24th of April, 2015. Kind regards, -- Ronnie Berntsson, PhD Assistant Professor Department of Medical Biochemistry and Biophysics Umeå University 90187 Umeå Sweden e-mail: ronnie.bernts...@medchem.umu.se
[ccp4bb] Choice of stereomicroscope
Dear all, I’m currently looking in to buying a new microscope for viewing crystal plates, mounting crystals etc, and would love some input into what I should get. What I would like is a microscope that has a high quality image, that is easy to work with and which is ergonomical. It does not have to have a fixed digital camera, but it should be possible to attach a digital camera to take pictures. Price is obviously also important... I’ve been looking at the standard microscopes that Molecular Dimensions sell, and also on a Nikon SMZ18. I remember that we used to have a Leica microscope in a previous lab that I liked, but can’t seem to find the model at the moment. I am also interested in getting a UV source, to inspect crystals under UV to see if you fluoresce (and hence are protein crystals). Molecular Dimension used to sell XtalLight 100, but doesn’t seem to do so anymore. Do any of you have other suggestions regarding the possibility of adding UV to a stereomicroscope? Suggestions and thoughts are more than welcome! Thanks, Ronnie -- Ronnie Berntsson, PhD Assistant Professor Department of Medical Biochemistry and Biophysics Umeå University 90187 Umeå Sweden e-mail: ronnie.bernts...@medchem.umu.se
Re: [ccp4bb] Choice of stereomicroscope
Ronnie I see a lot of cheap and expensive microscopes, and I notice that expensive is not always better for protein crystallization. Almost the most important thing is that illumination comes from *one particular direction*. Often this means that the light source is small and far from the sample stage. What does not work well is to have a large light source (eg multiple LEDs, large white screen, mirror or sintered transparent sheet) that is close to the sample - even with the best optics in the world, you won't see crystals well. Dark ground (see only scattered light) and ordinary transmission mode can both work well - good to have both if possible. Good luck, Patrick On 27 March 2015 at 13:08, Ronnie Berntsson ronnie.bernts...@medchem.umu.se wrote: Dear all, I’m currently looking in to buying a new microscope for viewing crystal plates, mounting crystals etc, and would love some input into what I should get. What I would like is a microscope that has a high quality image, that is easy to work with and which is ergonomical. It does not have to have a fixed digital camera, but it should be possible to attach a digital camera to take pictures. Price is obviously also important... I’ve been looking at the standard microscopes that Molecular Dimensions sell, and also on a Nikon SMZ18. I remember that we used to have a Leica microscope in a previous lab that I liked, but can’t seem to find the model at the moment. I am also interested in getting a UV source, to inspect crystals under UV to see if you fluoresce (and hence are protein crystals). Molecular Dimension used to sell XtalLight 100, but doesn’t seem to do so anymore. Do any of you have other suggestions regarding the possibility of adding UV to a stereomicroscope? Suggestions and thoughts are more than welcome! Thanks, Ronnie -- Ronnie Berntsson, PhD Assistant Professor Department of Medical Biochemistry and Biophysics Umeå University 90187 Umeå Sweden e-mail: ronnie.bernts...@medchem.umu.se -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36
[ccp4bb] Meeting announcement - Residential School on Medicinal Chemistry and Biology in Drug Discovery
All, This is a bit off topic, but I think this could be of general interest to folks.See below for information about the Residential School on Medicinal Chemistry and Biology in Drug Discovery. Sincerely, Corey Subject: Residential School on Medicinal Chemistry and Biology in Drug Discovery (ResMed): A Comprehensive One-Week Course Dear all, The 29th Annual ResMed: Residential School on Medicinal Chemistry and Biology in Drug Discovery will be held on June 7 - June 12, 2015 at Drew University in Madison, NJ and is co-sponsored by the ACS MEDI Division. ResMed is a week-long graduate level course organized to provide an accelerated program for chemists, biologists and other industrial and academic scientists who wish to broaden their knowledge of drug discovery and development. The School's aim is to concentrate on the fundamentals that are useful in drug discovery spanning initial target validation through clinical development. The program provides ample opportunity for discussions with distinguished faculty from industry and academia. The purpose of the School is to provide a strong background in the principles of drug discovery and development to enhance collaborative drug discovery programs. Previous attendees with backgrounds in macromolecular crystallography have found the course of great value in their research projects. Information regarding ResMed can be accessed on our website: www.drew.edu/resmedhttp://www.drew.edu/resmed including our program and application. Please join us in our 29th year. We look forward to seeing you. Sincerely, Co-organizers, William J. Greenlee, Ph.D Vincent P. Gullo, Ph.D. Ronald J. Doll, Ph.D. Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system.
[ccp4bb] First-Science Discussions for Life-Science Beamlines at NSLS-II: April 21-22, 2015
We will hold a First Science at the ABBIX Beamlines meeting on Tuesday - Wednesday, April 21-22, 2015, pertaining to the National Institutes of Health (NIH)-sponsored Advanced Beamlines for Biological Investigations with X-rays (ABBIX) Project. These three state-of-the-art beamlines are now under construction at the National Synchrotron Light Source (NSLS)-II facility at Brookhaven National Laboratory (BNL). Two of the beamlines are for macromolecular crystallography (MX), and the third is for life sciences applications of x-ray scattering. Visit this web site for further information and registration materials: http://workshops.ps.bnl.gov/?w=ABBIXApr2015 Briefly, the MX beamline called FMX - Frontier MX - is optimized to address microbeam applications, and the other called AMX - Highly Automated MX - is optimized for highly automated throughput. The scattering beamline called LIX - High Brightness X-Ray Scattering for Life Sciences - will offer multiple modes of study. The MX beamlines view canted undulator sources, and the scattering beamline views a single undulator, providing world-leading brightness. Current plans are to conclude construction by the end of 2015 and start commissioning then. During the commissioning period, opportunities will arise to perform early experiments before the general user programs of the beamlines commence in 2016, and it is the upcoming meeting's purpose to explore and discuss scientific areas and opportunities on which early operation of the beamlines could capitalize. Note: Registration is required. There is no registration fee. Visit this site to register: http://workshops.ps.bnl.gov/?w=ABBIXApr2015 Those who registered for the original meeting planned for January must re-register for the new occurrence in April. Contributed presentations describing experiments that exploit the capabilities of the ABBIX beamlines will be selected from submitted abstracts. The meeting will consist of both plenary and parallel sessions. The plenary session will begin with presentations on science topics in research fields that the beamlines are designed to address, followed by facility presentations to update the community about the early scientific capabilities at the suite of ABBIX beamlines and, in addition, of the Life Science and Biomedical Technology Research Resource (LSBR), sponsored by the NIH and the Department of Energy Office of Biological and Environmental Research (DOE BER). Two parallel sessions on Tuesday afternoon will focus on MX and x-ray scattering, consisting mainly of presentations that will address specific opportunities in these areas. These will be followed by two more parallel sessions on Wednesday morning, also focusing on MX and x-ray scattering, which will include contributions. If you wish to give a contributed presentation during one of the parallel sessions, please submit an abstract here: http://workshops.ps.bnl.gov/abstracts/default.aspx?w=ABBIXApr2015 The parallel sessions are designed to provide forums to facilitate more detailed discussions about potential first experiments at specific beamlines and the formation of the experiment teams. Specifically, each parallel session group will be charged to accomplish these goals: 1. Identify key research projects and the associated first experiments that may be performed at the suite of ABBIX beamlines, emphasizing those projects that will take advantage of NSLS-II properties and ABBIX beamline capabilities. 2. Facilitate the formation of research teams to generate first experiment proposals for submission. 3. Identify key additional team members in such areas as theory and analysis, ancillary measurements, and specimen preparation, needed to achieve the research goals. Confirmed invited speakers: Karen Allen (Boston Univ), Martin Caffrey (Trinity College Dublin), Seth Darst (Rockefeller Univ), Richard Gillilan (Cornell Univ), Wayne Hendrickson (Columbia Univ), AndrHoelz (California Inst Tech), Huey Huang (Rice Univ), Shenping Liu (Pfizer), Lee Makowski (Northeastern Univ), David Ostrov (Univ of Florida), Rebecca Page (Brown Univ), Anna Marie Pyle (Yale Univ), Christian Riekel (ESRF), Thomas Schneider (EMBL-Hamburg), John Tainer (Scripps Research Inst). We would like two outcomes from this meeting: (a) a concise meeting report outlining how ABBIX beamlines will help to address outstanding problems in life sciences, and (b) a list of potential first experiments along with the corresponding research teams. For more information on the meeting, please contact Sonya Kiss, at sk...@bnl.gov. Note: Registration deadline is Tuesday, April 14, 2015. Organizers: Lonny Berman ABBIX Project Manager National Synchrotron Light Source II Brookhaven National Laboratory Sean McSweeney Structural Biology Program Manager Photon Sciences Division Deputy Director National Synchrotron Light Source II
[ccp4bb] Scientific Sales Position - MiTeGen, LLC - Ithaca, NY
Dear All, We are excited to announce that we are hiring! We are looking to add a Scientific Sales Associate to our growing team. MiTeGen is focused on providing innovative solutions for all things crystallography. We continually look to optimize the tools, equipment, and processes used in crystallography research to improve the experimental reproducibility, ease of use, and quality of results generated. For all steps -- from crystallization through data collection -- we strive to make your research easier. If you are an energetic individual who would be excited to be part of a fast-paced technology company, we welcome you to apply. Learn more about this open position here: http://www.mitegen.com/careers/ Please feel free to direct any questions to me. Kind regards, Ben -- Benjamin Apker Director of Business Development MiTeGen 95 Brown Road, Suite 183 Ithaca, NY 14850 office: 607-266-8877 fax:607-697-0400 web: www.mitegen.com *Improve your research! Get crystallography related news, tips, and more by signing-up here: www.mitegen.com/newsletter http://www.mitegen.com/newsletter/?utm_source=bautm_medium=emailutm_campaign=newsletter * * http://www.mitegen.com/harvesting/poster/?utm_source=bautm_medium=emailutm_campaign=Posterutm_content=Crystal+Harvesting+Tips* ___ This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. MiTeGen, 95 Brown Road, Suite 183, Ithaca,NY 14850.
