[ccp4bb] HTS Specialist Position at St. Jude

[Marcus Fischer]

The High Throughput Biosciences (HTB) Center is recruiting a senior-level
scientist to serve as our in-house protein expert and to join our efforts
in using high-throughput screening (HTS) for structure/target-based small
molecule discovery (Job title: HTS Specialist). The HTS Specialist reports
to the Director of the High Throughput Bioscience (HTB) Center and is
expected to independently lead and implement collaborative projects;
present and publish research results; and assist the Center Director in
managing the daily operations of the Center.



The successful candidate will have extensive experience in molecular
biology, protein biochemistry, and structural biology and a strong interest
in learning, developing, and implementing new techniques related to
high-throughput small-molecule drug discovery.



Experience in the following areas is a plus: protein purification, protein
crystallography, docking, and biochemical and biophysical assay
development, along with a strong publication record.



Please direct your questions, cover letter, current CV, and 3 letters of
reference to Dr. Taosheng Chen (taosheng.c...@stjude.org).



The *High Throughput Bioscience Center* at St. Jude consists of members
with advanced training in biology, chemistry, and engineering. We have
professional experience in target identification and validation, assay
development, high-throughput screening, laboratory automation, and
management of scientific collaborations.



*Minimum Education*

Bachelor's or Master's degree in biology, chemistry, or a related field
required. PhD in biological sciences with relevant postdoctoral training
preferred.



*Minimum Experience *

Seven (7) years of relevant experience is required with a Bachelor’s
degree.

Six (6) years of relevant experience is acceptable with a Master’s degree.

At least two (2) years of relevant experience is acceptable with a PhD.


*Contact*

Taosheng Chen, PhD

Member, Department of Chemical Biology and Therapeutics

Director, High Throughput Bioscience Center

St. Jude Children's Research Hospital

Memphis, TN, 38105-3678, USA

https://www.stjude.org/chen

http://www.stjuderesearch.org/site/lab/chen



*Link to Ad*

https://careers-stjude.icims.com/jobs/3973/hts-specialist/job



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Re: [ccp4bb] Sulphate or phosphate?

[Eleanor Dodson]

I am not sure if you gave your wave length but it is always worth doing an
anomalous map, and looking at relativr peak heights for your known S
positions and the putative sulphate or phosphate.
There are small differences in the expected f" at most wavelengths.

Chemical arguments are doubtless better of Course!
Eleanor

On 1 August 2018 at 16:54, armin.wag...@diamond.ac.uk <
armin.wag...@diamond.ac.uk> wrote:

> Dear David,
>
> The long-wavelength beamline I23 at Diamond (http://www.diamond.ac.uk/
> Instruments/Mx/I23.html) can go all way down to the phosphorous edge.
> While data quality will be obviously compromised by absorption effects (we
> are working on this), for a reasonably well diffracting crystal like yours
> we should be able to get anomalous difference fourier maps from data
> collected above and below the sulphur edge (lambda ~5 A) to answer your
> question.
>
> We’ve published some test crystal work around the sulphur edge a while
> ago, you might want to have a look here:
> https://doi.org/10.1016/j.nimb.2016.12.005
>
> The beamline is currently in an advanced commissioning phase accepting a
> limited number of projects. So, please get into touch if you are
> interested. We are currently sorting out a few more hardware and software
> aspects before we can run the beamline as a standard user facility, but we
> have made a lot of progress over the past months. There will be more
> details here on the bulletin board ahead of the next call for proposals in
> autumn.
>
> Best regards,
>
> Armin
>
>
> Postdoc position for long-wavelength crystallography:
> https://vacancies.diamond.ac.uk/vacancy/post-doctoral-
> research-associate-355285.html
>
>
>
>
> On 31/07/2018, 14:38, "CCP4 bulletin board on behalf of David Schuller" <
> CCP4BB@JISCMAIL.AC.UK on behalf of schul...@cornell.edu> wrote:
>
> How can one distinguish between a sulphate or phosphate in an electron
> density map? Both are present in the mother liquor, and resolution is
> in
> the range of 1.75 - 2.25 A
>
>
> --
> 
> ===
> All Things Serve the Beam
> 
> ===
> David J. Schuller
> modern man in a post-modern world
> MacCHESS, Cornell University
> schul...@cornell.edu
>
> 
> 
>
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
>
>
>
> --
> This e-mail and any attachments may contain confidential, copyright and or
> privileged material, and are for the use of the intended addressee only. If
> you are not the intended addressee or an authorised recipient of the
> addressee please notify us of receipt by returning the e-mail and do not
> use, copy, retain, distribute or disclose the information in or attached to
> the e-mail.
> Any opinions expressed within this e-mail are those of the individual and
> not necessarily of Diamond Light Source Ltd.
> Diamond Light Source Ltd. cannot guarantee that this e-mail or any
> attachments are free from viruses and we cannot accept liability for any
> damage which you may sustain as a result of software viruses which may be
> transmitted in or with the message.
> Diamond Light Source Limited (company no. 4375679). Registered in England
> and Wales with its registered office at Diamond House, Harwell Science and
> Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom
>
>
> 
>
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>



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[ccp4bb] Postdoctoral position in ion channel structure-function available in the laboratory of Crina Nimigean at Weill Cornell Medical College in New York City

[Crina Nimigean]

Postdoctoral position in ion channel structure/function available in the 
laboratory of Crina Nimigean at Weill Cornell Medical College in New York City
There is an availability in the laboratory of Crina Nimigean for an 
enthusiastic postdoc who is interested in the molecular workings of ion 
channels. The successful candidate will investigate ion channel structure and 
mechanism with single-particle cryo-EM and functional assays such as 
single-channel recordings, stopped-flow fluorescence, etc. The work in the lab 
is generally geared towards developing a mechanistic understanding of ion 
channels using functional and structural techniques. The ideal candidate for 
this position is an ion channel electrophysiologist/biochemist who wants to 
gain experience in cryo-EM.

We are located at the Weill Cornell Medical College on the upper east side of 
Manhattan, within the vibrant and international tri-Institutional scientific 
community, which is comprised of Rockefeller University, Memorial Sloan 
Kettering and Weill Cornell. We have screening electron microscopes on site, 
within the newly established cryo-EM core facility at Cornell, and we have full 
access to state-of-the-art Titan Krios microscopes for high resolution data 
collection at the New York Structural Biology Center.

Qualifications and experience: Candidates should hold a Ph.D. and have a solid 
background in biophysics, ion channel electrophysiology, and/or protein 
biochemistry.  Experience with cryo-EM is thus not necessary. Excellent verbal 
and written English communication skills, and ability to work in close 
collaboration with other researchers are required.
Qualified applicants should send a cover letter, CV, and the names of three 
references by email to Crina Nimigean at 
crn2...@med.cornell.edu.


-
Crina Nimigean, Ph.D.
Weill Cornell Medical College
Associate Professor of Physiology and Biophysics in Anesthesiology
Associate Professor of Biochemistry
http://physiology.med.cornell.edu/faculty/nimigean/lab/

Department of Anesthesiology, Box 124
525 East 68th Street, Room A-1050
New York, NY 10065
Phone: (212) 746 5947
Fax: (212) 746 4879




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[ccp4bb] Imaging data analysis position at Diamond Light source Beamline B24

[maria.harkiol...@diamond.ac.uk]

Dear all,


The X-ray tomography and super resolution fluorescence microscopy beamline B24 
at Diamond Light Source (the UK synchrotron) are looking for a highly motivated 
data analysis scientist to join their team. The successful candidate will 
contribute to the ongoing development of methods to improve the resident 
imaging capabilities, and will be involved in a number of beamline projects.


Further information can be found at:


https://vacancies.diamond.ac.uk/vacancy/software-data-analysis-scientist-353613.html

Kind regards,


Maria

-- 
This e-mail and any attachments may contain confidential, copyright and or 
privileged material, and are for the use of the intended addressee only. If you 
are not the intended addressee or an authorised recipient of the addressee 
please notify us of receipt by returning the e-mail and do not use, copy, 
retain, distribute or disclose the information in or attached to the e-mail.
Any opinions expressed within this e-mail are those of the individual and not 
necessarily of Diamond Light Source Ltd. 
Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments 
are free from viruses and we cannot accept liability for any damage which you 
may sustain as a result of software viruses which may be transmitted in or with 
the message.
Diamond Light Source Limited (company no. 4375679). Registered in England and 
Wales with its registered office at Diamond House, Harwell Science and 
Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom




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Re: [ccp4bb] Sulphate or phosphate?

[armin.wag...@diamond.ac.uk]

Dear David,

The long-wavelength beamline I23 at Diamond 
(http://www.diamond.ac.uk/Instruments/Mx/I23.html) can go all way down to the 
phosphorous edge. While data quality will be obviously compromised by 
absorption effects (we are working on this), for a reasonably well diffracting 
crystal like yours we should be able to get anomalous difference fourier maps 
from data collected above and below the sulphur edge (lambda ~5 A) to answer 
your question.

We’ve published some test crystal work around the sulphur edge a while ago, you 
might want to have a look here:
https://doi.org/10.1016/j.nimb.2016.12.005

The beamline is currently in an advanced commissioning phase accepting a 
limited number of projects. So, please get into touch if you are interested. We 
are currently sorting out a few more hardware and software aspects before we 
can run the beamline as a standard user facility, but we have made a lot of 
progress over the past months. There will be more details here on the bulletin 
board ahead of the next call for proposals in autumn.

Best regards,

Armin


Postdoc position for long-wavelength crystallography:
https://vacancies.diamond.ac.uk/vacancy/post-doctoral-research-associate-355285.html




On 31/07/2018, 14:38, "CCP4 bulletin board on behalf of David Schuller" 
 wrote:

How can one distinguish between a sulphate or phosphate in an electron 
density map? Both are present in the mother liquor, and resolution is in 
the range of 1.75 - 2.25 A


-- 
===
All Things Serve the Beam
===
David J. Schuller
modern man in a post-modern world
MacCHESS, Cornell University
schul...@cornell.edu



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-- 
This e-mail and any attachments may contain confidential, copyright and or 
privileged material, and are for the use of the intended addressee only. If you 
are not the intended addressee or an authorised recipient of the addressee 
please notify us of receipt by returning the e-mail and do not use, copy, 
retain, distribute or disclose the information in or attached to the e-mail.
Any opinions expressed within this e-mail are those of the individual and not 
necessarily of Diamond Light Source Ltd. 
Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments 
are free from viruses and we cannot accept liability for any damage which you 
may sustain as a result of software viruses which may be transmitted in or with 
the message.
Diamond Light Source Limited (company no. 4375679). Registered in England and 
Wales with its registered office at Diamond House, Harwell Science and 
Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom




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[ccp4bb] Postdoc position available

[Xiao Lei]

*Postdoctoral Scholar Position on Investigating CRISPR Mechanism at
University of Southern California, Los Angeles, California, USA*



The Qin Laboratory in the Chemistry Department of the University of
Southern California invites applications for postdoctoral scholar positions
supported by funding from NIH and NSF. The work centers on investigating
mechanisms of target recognition by the programmable CRISPR nucleases that
are revolutionizing genome engineering. The projects use a combination of
biophysical techniques, including spin-labeling and EPR (a specialty of the
Qin lab, seehttp://pzqin.usc.edu/pzqhome/), fluorescence, and structural
characterization. Candidates with prior experience on protein expression,
mutagenesis, structural analyses (e.g., X-ray, NMR), and protein-nucleic
acid interaction are particularly welcome. Strong writing and communication
skills, as evidenced by first author publications, are highly desirable.



The appointment is available initially for three years contingent on
satisfactory research progress. Funding starts in Fall 2018, and salary
will commensurate with qualifications. To Apply, please email a cover
letter, CV and contact information for three references to Professor Peter
Qin, p...@usc.edu

*(PS.  Please do not reply to this email, I post this job for Dr. Peter
Qin, please directly contact with Dr. Peter Qin)*



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Re: [ccp4bb] identifying bound ions

[Kay Diederichs]

Dear Herman,

the "water scrutinizer" option of XPAND does this -. 
http://www.msg.ucsf.edu/local/programs/ono/manuals/xpand_man.html#S7

best wishes,

Kay

On Tue, 31 Jul 2018 12:39:01 +, herman.schreu...@sanofi.com wrote:

>Dear BB,
>
>I know it has been discussed some time ago, but a google search did not come 
>up with anything useful.
>
>I need a program which analyzes the bound waters and suggests whether a 
>particular water might be a chloride, calcium, sulfate, sodium or something 
>else. Preferably a program that can be run off-line (not a web server), but if 
>there is no choice, we will use a webserver as well.
>
>Thank you for your suggestions!
>Herman
>
>
>
>
>
>
>To unsubscribe from the CCP4BB list, click the following link:
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>



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