Re: [ccp4bb] Interesting pattern on a crystallization drop

[Philippe BENAS]

Hi Beatriz,
 
Very interesting indeed. Couldn't it be the ghost printing of some mechanical 
part used to make the mould of the crystallization tray ?
All the best,
Philippe

Philippe BENAS, Ph.D.

ARN UPR 9002 CNRS
IBMC Strasbourg
15, rue René Descartes
F-67084 STRASBOURG cedex
+33.3.8841.7109
E-mails: p.be...@ibmc-cnrs.unistra.fr, philippe_be...@yahoo.fr
URLs:   http://www-ibmc.u-strasbg.fr/ , http://www-ibmc.u-strasbg.fr/spip-arn/

 

Le mercredi 27 mars 2019 à 19:54:42 UTC+1, Beatriz Gomes Guimaraes 
 a écrit :  
 
  
Dear all,




I would like to share with you a surprising pattern I found when examining some 
crystallization plates (attached figures).




It is less obvious looking the photos, but apparently the "lines" are formed by 
precipitated protein and there are some "bubbles" with small drops inside.I 
wish they were microcrystals but I do not think this is the case.


I was suprised by the symmetry !




And it is not completely random because for the same condition the difference 
between the two drops are : protein alone ("hexagon") and protein + ligand 
("rhombus")



crystallization condition is:

0.01 M Cobalt(II) chloride hexahydrate

0.1 M Tris pH 8.5

20% w/v Polyvinylpyrrolidone K 15





Have you seen anything similar before?




Thank you for your comments!

Beatriz






--
Beatriz Guimarães
Laboratory of Structural Biology and Protein Engineering
Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
Rua Prof. Algacyr Munhoz Mader, 3775   Bloco C
CIC 81350-010
Curitiba - PR, Brasil
Tel.:+55(41)3316-3225/2104-3438

To unsubscribe from the CCP4BB list, click the following link:
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Re: [ccp4bb] Interesting pattern on a crystallization drop

[Sergei Strelkov]

Artem (and Beatriz),


Me bad, could have thought about that! I think you are right.


There were initially bubbles in each drop (7 in one case, 4 in the other).

At some point the bubbles exploded (it was an instantaneous process, not just 
shrinking).


Kind regards,

Sergei


Prof. Sergei V. Strelkov Laboratory for Biocrystallography Department of 
Pharmaceutical Sciences, KU Leuven O&N2, Campus Gasthuisberg, Herestraat 49 bus 
822, 3000 Leuven, Belgium Phone: +32 16 33 08 45, mobile: +32 486 29 41 32 Lab 
pages: 
http://pharm.kuleuven.be/Biocrystallography


From: CCP4 bulletin board  on behalf of Artem Evdokimov 

Sent: Thursday, March 28, 2019 1:07
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop

Neat!

Looks like multiple adjacent bubbles that were initially touching but 
eventually shrunk down to the central cores - the connectors are protein 
filaments (skin on the bubbles) left over from when bubbles had contact points.

Artem

On Wed, Mar 27, 2019, 19:39 Marshall, Bevan (Manufacturing, Parkville) 
 wrote:
Looked up the condition on C6 (https://c6.csiro.au/C6.asp) and that condition 
is found in both Index and JCSG screens as well as Classics II.


Bevan Marshall
Staff Scientist | Collaborative Crystallisation Centre
Manufacturing
CSIRO
E bevan.marsh...@csiro.auT +61 3 9662 7492
343-351 Royal Parade, Parkville, VIC 3052
www.csiro.au | https://c3.csiro.au
CSIRO acknowledges the Traditional Owners of the lands that we live and work on 
across Australia and pays its respect to Elders past and present.
PLEASE NOTE
The information contained in this email may be confidential or privileged. Any 
unauthorised use or disclosure is prohibited. If you have received this email 
in error, please delete it immediately and notify the sender by return email. 
Thank you. To the extent permitted by law, CSIRO does not represent, warrant 
and/or guarantee that the integrity of this communication has been maintained 
or that the communication is free of errors, virus, interception or 
interference.
Please consider the environment before printing this email.

From: CCP4 bulletin board 
[mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of 
LEGRAND Pierre
Sent: Thursday, 28 March 2019 9:13 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop

Dear Beatriz,

Nice drops :-))
Could it be that there is a reaction going on in these drops ?
The conditions are quite "exotic" with possibilities of coordination or 
oxydoreduction (Co2+/Co3+) or polymerization...
Do you have reductants with the protein buffer ?
Is the protein an enzyme or a metalloprotein ?
Just some ideas.

Best wishes,
Pierre


De : CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] 
de la part de Beatriz Gomes Guimaraes 
[beatriz.guimar...@fiocruz.br]
Envoyé : mercredi 27 mars 2019 19:44
À : CCP4BB@JISCMAIL.AC.UK
Objet : [ccp4bb] Interesting pattern on a crystallization drop

Dear all,



I would like to share with you a surprising pattern I found when examining some 
crystallization plates (attached figures).



It is less obvious looking the photos, but apparently the "lines" are formed by 
precipitated protein and there are some "bubbles" with small drops inside. I 
wish they were microcrystals but I do not think this is the case.

I was suprised by the symmetry !



And it is not completely random because for the same condition the difference 
between the two drops are : protein alone ("hexagon") and protein + ligand 
("rhombus")


crystallization condition is:

0.01 M Cobalt(II) chloride hexahydrate

0.1 M Tris pH 8.5

20% w/v Polyvinylpyrrolidone K 15



Have you seen anything similar before?



Thank you for your comments!

Beatriz




--
Beatriz Guimarães
Laboratory of Structural Biology and Protein Engineering
Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
Rua Prof. Algacyr Munhoz Mader, 3775   Bloco C
CIC 81350-010
Curitiba - PR, Brasil
Tel.:+55(41)3316-3225/2104-3438



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#

Re: [ccp4bb] Interesting pattern on a crystallization drop

[jai mohan]

 May be, I do co-relate your crystal pic with Manu Prakash at Stanford on his 
work on Dancing Droplets, briefing the surface tension and evaporation ^ the 
rule of two component fluids. # Since your precipitant contain PVP a shape 
controlling agent 
#https://med.stanford.edu/news/all-news/2015/03/researchers-solve-mystery-of-the-dancing-droplets.html

Best wishes
S.M.Jaimohan PhD
On Thursday, 28 March, 2019, 1:54:23 pm IST, Sergei Strelkov 
 wrote:  
 
 #yiv3861306982 #yiv3861306982 --P{margin-top:0;margin-bottom:0;}#yiv3861306982 
Artem (and Beatriz),





Me bad, could have thought about that! I think you are right.




There were initially bubbles in each drop (7 in one case, 4 in the other).

At some point the bubbles exploded (it was an instantaneous process, not just 
shrinking).




Kind regards,

Sergei




Prof. Sergei V. Strelkov Laboratory for Biocrystallography Department of 
Pharmaceutical Sciences, KU Leuven O&N2, Campus Gasthuisberg, Herestraat 49 bus 
822, 3000 Leuven, Belgium Phone: +32 16 33 08 45, mobile: +32 486 29 41 32 Lab 
pages: http://pharm.kuleuven.be/BiocrystallographyFrom: CCP4 bulletin board 
 on behalf of Artem Evdokimov 
Sent: Thursday, March 28, 2019 1:07
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop Neat!
Looks like multiple adjacent bubbles that were initially touching but 
eventually shrunk down to the central cores - the connectors are protein 
filaments (skin on the bubbles) left over from when bubbles had contact points.
Artem
On Wed, Mar 27, 2019, 19:39 Marshall, Bevan (Manufacturing, Parkville) 
 wrote:


Looked up the condition on C6 (https://c6.csiro.au/C6.asp) and that condition 
is found in both Index and JCSG screens as well as Classics II.

 

 

Bevan Marshall
Staff Scientist | Collaborative Crystallisation Centre
Manufacturing
CSIRO

E bevan.marsh...@csiro.aut +61 3 9662 7492  
343-351 Royal Parade, Parkville, VIC 3052
www.csiro.au|https://c3.csiro.au 
CSIRO acknowledges the Traditional Owners of the lands that we live and work on 
across Australia and pays its respect to Elders past and present.

PLEASE NOTE
The information contained in this email may be confidential or privileged. Any 
unauthorised use or disclosure is prohibited. If you have received this email 
in error, please delete it immediately and notify the sender by return email. 
Thank you. To the extent permitted by law, CSIRO does not represent, warrant 
and/or guarantee that the integrity of this communication has been maintained 
or that the communication is free of errors, virus, interception or 
interference.

Please consider the environment before printing this email.

 

From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK]On Behalf Of LEGRAND 
Pierre
Sent: Thursday, 28 March 2019 9:13 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop

 

Dear Beatriz,

 

Nice drops :-))

Could it be that there is a reaction going on in these drops ?

The conditions are quite "exotic" with possibilities of coordination or 
oxydoreduction (Co2+/Co3+) or polymerization...

Do you have reductants with the protein buffer ?

Is the protein an enzyme or a metalloprotein ?

Just some ideas.

 

Best wishes,

Pierre

 

De : CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] de la part de Beatriz Gomes 
Guimaraes [beatriz.guimar...@fiocruz.br]
Envoyé : mercredi 27 mars 2019 19:44
À : CCP4BB@JISCMAIL.AC.UK
Objet : [ccp4bb] Interesting pattern on a crystallization drop

Dear all,

 

I would like to share with you a surprising pattern I found when examining some 
crystallization plates (attached figures).

 

It is less obvious looking the photos, but apparently the "lines" are formed by 
precipitated protein and there are some "bubbles" with small drops inside.I 
wish they were microcrystals but I do not think this is the case.

I was suprised by the symmetry !

 

And it is not completely random because for the same condition the difference 
between the two drops are : protein alone ("hexagon") and protein + ligand 
("rhombus")

 

crystallization condition is:

0.01 M Cobalt(II) chloride hexahydrate

0.1 M Tris pH 8.5

20% w/v Polyvinylpyrrolidone K 15

 

Have you seen anything similar before?

 

Thank you for your comments!

Beatriz

 

 

--
Beatriz Guimarães
Laboratory of Structural Biology and Protein Engineering
Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
Rua Prof. Algacyr Munhoz Mader, 3775   Bloco C
CIC 81350-010
Curitiba - PR, Brasil
Tel.:+55(41)3316-3225/2104-3438

 

To unsubscribe from the CCP4BB list, click the following link:
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[ccp4bb] Postdoctoral Fellow Position at University of Delaware

[Vijay Parashar]

Postdoctoral Fellow Position

Parashar laboratory at University of Delaware College of Health Sciences, is 
currently seeking a Postdoctoral Fellow to perform structure-function studies 
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·  Experience with cloning, protein expression, and protein purification 
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·  A sound understanding of methods and approaches relating to 
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Parashar laboratory is well equipped with state-of-the-art equipment for 
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access to an X-ray generator). Please visit Parashar Laboratory 
 website for more 
information. For consideration, please send your CV, list of three references 
and other relevant information to paras...@udel.edu  
with subject line "postdoctoral application"


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[ccp4bb] Postdoctoral Position Available at Rutgers University

[Matthew Neiditch]

An NIH-funded postdoctoral position is available at Rutgers University - New 
Jersey Medical School to study the structural basis of bacterial competence, 
more specifically the movement of DNA across bacterial membranes during natural 
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the subject line Postdoctoral Position Applicant.

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Associate Professor
Dept. of Microbiology and Molecular Genetics
New Jersey Medical School
Rutgers University

David Dubnau, Ph.D.
Professor
Public Health Research Institute
New Jersey Medical School
Rutgers University



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Re: [ccp4bb] Interesting pattern on a crystallization drop

[IGBMC]

Le Jeudi 28 Mars 2019 12:03 CET, jai mohan 
<0cab66323371-dmarc-requ...@jiscmail.ac.uk> a écrit:

A friend of mine (Angel Piñeiro) just suggested to me the "Maragoni effect", 
which is likely active in the dancing droplets from Stanford and might explain 
the "explosion" into separate bubbles
Indeed, one can see on Wikipedia a video illustrating how the contact of fluids 
with different surface tensions can have big effects...


>  May be, I do co-relate your crystal pic with Manu Prakash at Stanford on his 
> work on Dancing Droplets, briefing the surface tension and evaporation ^ the 
> rule of two component fluids. # Since your precipitant contain PVP a shape 
> controlling agent 
> #https://med.stanford.edu/news/all-news/2015/03/researchers-solve-mystery-of-the-dancing-droplets.html
>
> Best wishes
> S.M.Jaimohan PhD
> On Thursday, 28 March, 2019, 1:54:23 pm IST, Sergei Strelkov 
>  wrote:
>
>  #yiv3861306982 #yiv3861306982 
> --P{margin-top:0;margin-bottom:0;}#yiv3861306982
> Artem (and Beatriz),
>
>
>
>
>
> Me bad, could have thought about that! I think you are right.
>
>
>
>
> There were initially bubbles in each drop (7 in one case, 4 in the other).
>
> At some point the bubbles exploded (it was an instantaneous process, not just 
> shrinking).
>
>
>
>
> Kind regards,
>
> Sergei
>
>
>
>
> Prof. Sergei V. Strelkov Laboratory for Biocrystallography Department of 
> Pharmaceutical Sciences, KU Leuven O&N2, Campus Gasthuisberg, Herestraat 49 
> bus 822, 3000 Leuven, Belgium Phone: +32 16 33 08 45, mobile: +32 486 29 41 
> 32 Lab pages: http://pharm.kuleuven.be/BiocrystallographyFrom: CCP4 bulletin 
> board  on behalf of Artem Evdokimov 
> 
> Sent: Thursday, March 28, 2019 1:07
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop Neat!
> Looks like multiple adjacent bubbles that were initially touching but 
> eventually shrunk down to the central cores - the connectors are protein 
> filaments (skin on the bubbles) left over from when bubbles had contact 
> points.
> Artem
> On Wed, Mar 27, 2019, 19:39 Marshall, Bevan (Manufacturing, Parkville) 
>  wrote:
>
>
> Looked up the condition on C6 (https://c6.csiro.au/C6.asp) and that condition 
> is found in both Index and JCSG screens as well as Classics II.
>
>  
>
>  
>
> Bevan Marshall
> Staff Scientist | Collaborative Crystallisation Centre
> Manufacturing
> CSIRO
>
> E bevan.marsh...@csiro.aut +61 3 9662 7492 
> 343-351 Royal Parade, Parkville, VIC 3052
> www.csiro.au|https://c3.csiro.au 
> CSIRO acknowledges the Traditional Owners of the lands that we live and work 
> on across Australia and pays its respect to Elders past and present.
>
> PLEASE NOTE
> The information contained in this email may be confidential or privileged. 
> Any unauthorised use or disclosure is prohibited. If you have received this 
> email in error, please delete it immediately and notify the sender by return 
> email. Thank you. To the extent permitted by law, CSIRO does not represent, 
> warrant and/or guarantee that the integrity of this communication has been 
> maintained or that the communication is free of errors, virus, interception 
> or interference.
>
> Please consider the environment before printing this email.
>
>  
>
> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK]On Behalf Of LEGRAND 
> Pierre
> Sent: Thursday, 28 March 2019 9:13 AM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop
>
>  
>
> Dear Beatriz,
>
>  
>
> Nice drops :-))
>
> Could it be that there is a reaction going on in these drops ?
>
> The conditions are quite "exotic" with possibilities of coordination or 
> oxydoreduction (Co2+/Co3+) or polymerization...
>
> Do you have reductants with the protein buffer ?
>
> Is the protein an enzyme or a metalloprotein ?
>
> Just some ideas.
>
>  
>
> Best wishes,
>
> Pierre
>
>  
>
> De : CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] de la part de Beatriz Gomes 
> Guimaraes [beatriz.guimar...@fiocruz.br]
> Envoyé : mercredi 27 mars 2019 19:44
> À : CCP4BB@JISCMAIL.AC.UK
> Objet : [ccp4bb] Interesting pattern on a crystallization drop
>
> Dear all,
>
>  
>
> I would like to share with you a surprising pattern I found when examining 
> some crystallization plates (attached figures).
>
>  
>
> It is less obvious looking the photos, but apparently the "lines" are formed 
> by precipitated protein and there are some "bubbles" with small drops 
> inside.I wish they were microcrystals but I do not think this is the case.
>
> I was suprised by the symmetry !
>
>  
>
> And it is not completely random because for the same condition the difference 
> between the two drops are : protein alone ("hexagon") and protein + ligand 
> ("rhombus")
>
>  
>
> crystallization condition is:
>
> 0.01 M Cobalt(II) chloride hexahydrate
>
> 0.1 M Tris pH 8.5
>
> 20% w/v Polyvinylpyrrolidone K 15
>
>  
>
> Have you seen anything similar before?
>
>  
>
> Thank you for your comm

Re: [ccp4bb] Interesting pattern on a crystallization drop

[Beatriz Gomes Guimaraes]

Thank you all for the interesting comments and information about bubbles 
behavior and dancing droplets!


Unfortunately, for now, I won´t be able to further investigate the process (by 
reproducing the crystallization setup under a camera as suggested by Philippe 
Dumas), but I will keep this in mind.


Meanwhile, I keep trying to get crystals!


All the best,

Beatriz




--
Beatriz Guimarães
Laboratory of Structural Biology and Protein Engineering
Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
Rua Prof. Algacyr Munhoz Mader, 3775   Bloco C
CIC 81350-010
Curitiba - PR, Brasil
Tel.:+55(41)3316-3225/2104-3438



De: CCP4 bulletin board  em nome de DUMAS Philippe 
(IGBMC) 
Enviado: quinta-feira, 28 de março de 2019 12:48
Para: CCP4BB@JISCMAIL.AC.UK
Assunto: Re: [ccp4bb] Interesting pattern on a crystallization drop


Le Jeudi 28 Mars 2019 12:03 CET, jai mohan 
<0cab66323371-dmarc-requ...@jiscmail.ac.uk> a écrit:

A friend of mine (Angel Piñeiro) just suggested to me the "Maragoni effect", 
which is likely active in the dancing droplets from Stanford and might explain 
the "explosion" into separate bubbles
Indeed, one can see on Wikipedia a video illustrating how the contact of fluids 
with different surface tensions can have big effects...


>  May be, I do co-relate your crystal pic with Manu Prakash at Stanford on his 
> work on Dancing Droplets, briefing the surface tension and evaporation ^ the 
> rule of two component fluids. # Since your precipitant contain PVP a shape 
> controlling agent 
> #https://med.stanford.edu/news/all-news/2015/03/researchers-solve-mystery-of-the-dancing-droplets.html
>
> Best wishes
> S.M.Jaimohan PhD
> On Thursday, 28 March, 2019, 1:54:23 pm IST, Sergei Strelkov 
>  wrote:
>
>  #yiv3861306982 #yiv3861306982 
> --P{margin-top:0;margin-bottom:0;}#yiv3861306982
> Artem (and Beatriz),
>
>
>
>
>
> Me bad, could have thought about that! I think you are right.
>
>
>
>
> There were initially bubbles in each drop (7 in one case, 4 in the other).
>
> At some point the bubbles exploded (it was an instantaneous process, not just 
> shrinking).
>
>
>
>
> Kind regards,
>
> Sergei
>
>
>
>
> Prof. Sergei V. Strelkov Laboratory for Biocrystallography Department of 
> Pharmaceutical Sciences, KU Leuven O&N2, Campus Gasthuisberg, Herestraat 49 
> bus 822, 3000 Leuven, Belgium Phone: +32 16 33 08 45, mobile: +32 486 29 41 
> 32 Lab pages: http://pharm.kuleuven.be/BiocrystallographyFrom: CCP4 bulletin 
> board  on behalf of Artem Evdokimov 
> 
> Sent: Thursday, March 28, 2019 1:07
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop Neat!
> Looks like multiple adjacent bubbles that were initially touching but 
> eventually shrunk down to the central cores - the connectors are protein 
> filaments (skin on the bubbles) left over from when bubbles had contact 
> points.
> Artem
> On Wed, Mar 27, 2019, 19:39 Marshall, Bevan (Manufacturing, Parkville) 
>  wrote:
>
>
> Looked up the condition on C6 (https://c6.csiro.au/C6.asp) and that condition 
> is found in both Index and JCSG screens as well as Classics II.
>
>
>
>
>
> Bevan Marshall
> Staff Scientist | Collaborative Crystallisation Centre
> Manufacturing
> CSIRO
>
> E bevan.marsh...@csiro.aut +61 3 9662 7492
> 343-351 Royal Parade, Parkville, VIC 3052
> www.csiro.au|https://c3.csiro.au
> CSIRO acknowledges the Traditional Owners of the lands that we live and work 
> on across Australia and pays its respect to Elders past and present.
>
> PLEASE NOTE
> The information contained in this email may be confidential or privileged. 
> Any unauthorised use or disclosure is prohibited. If you have received this 
> email in error, please delete it immediately and notify the sender by return 
> email. Thank you. To the extent permitted by law, CSIRO does not represent, 
> warrant and/or guarantee that the integrity of this communication has been 
> maintained or that the communication is free of errors, virus, interception 
> or interference.
>
> Please consider the environment before printing this email.
>
>
>
> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK]On Behalf Of LEGRAND 
> Pierre
> Sent: Thursday, 28 March 2019 9:13 AM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop
>
>
>
> Dear Beatriz,
>
>
>
> Nice drops :-))
>
> Could it be that there is a reaction going on in these drops ?
>
> The conditions are quite "exotic" with possibilities of coordination or 
> oxydoreduction (Co2+/Co3+) or polymerization...
>
> Do you have reductants with the protein buffer ?
>
> Is the protein an enzyme or a metalloprotein ?
>
> Just some ideas.
>
>
>
> Best wishes,
>
> Pierre
>
>
>
> De : CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] de la part de Beatriz Gomes 
> Guimaraes [beatriz.guimar...@fiocruz.br]
> Envoyé : mercredi 27 mars 2019 19:44
> À : CCP4BB@JIS