Re: [ccp4bb] Does MicroED (microcrystal electron diffraction) have phase problem ?

[Edward A. Berry]

For my own info,
Does microED encompass work with 2D crystals, or only micro-3D crystals?

On 08/15/2020 10:40 PM, Jessica Bruhn wrote:

Hi Alex,

Welcome to the field of microED! From a practical standpoint, microED also 
suffers from the phase problem, and somewhat moreso compared to X-ray 
crystallography because anomalous signal is very limited. It is true that a TEM 
microscope operated in imaging mode produces images that contain both phase and 
amplitude information, which you correctly infer means that single particle 
cryoEM does not suffer from the phase problem. This is why a map from cryoEM is 
generally of higher quality than one determined by X-ray crystallography at the 
same resolution, because we don't have to guess at the phases.

In classic microED data collection, we don't actually take advantage of the 
phase information that can be measured using imaging mode. We quickly find our 
crystals in imaging mode and then flip the switch to diffraction mode and 
collect a dataset devoid of phase information. It has been suggested that we 
could use imaging mode to get at the phases of the diffraction patterns, but I 
am not aware of anyone actually doing this. Practically speaking, this would 
add significant additional time to the data collection and we likely would only 
be able to reliably use the phases for the lower resolution range (though, that 
might not be a deal breaker). Thankfully though, molecular replacement and ab 
initio methods for small molecules work pretty well on these datasets. Plus, 
most X-ray structures are solved this way anyways.

There have been efforts to use radiation damage as a means to phase a small peptide bound to zinc 
(https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7313391/ 
).
 And there is some interesting work being done with dynamical scattering as a way to see differences 
in Friedel pairs (see Tim Gruene's post here: 
https://www.jiscmail.ac.uk/cgi-bin/wa-jisc.exe?A2=CCP4BB;b22eae56.2007 
),
 but these methods are not "classic microED" and require completely different data 
processing software that is unfamiliar to most X-ray crystallographers.

For now, we just crank up the cycles in SHELXT/SHELXD or hope for a good 
molecular replacement model.

Best of luck,
Jessica

On Sat, Aug 15, 2020 at 6:03 PM Alex Lee mailto:alexlee198...@gmail.com>> wrote:

Hi, All,

I am new to MicroED (microcrystal electron diffraction). I know that X-ray 
crystallography has phase problem, and I think MicroED has phase problem too 
(it is diffraction of electron instead of x-ray). However, when I read the 
Wikipedia, I could not understand the following description of MicroED: One of 
the main difficulties in X-ray crystallography is determining phases in the 
diffraction pattern. Because of the complexity of X-ray lenses, it is difficult 
to form an image of the crystal being diffracted, and hence phase information 
is lost. Fortunately, electron microscopes can resolve atomic structure in real 
space and the crystallographic structure factor phase information can be 
experimentally determined from an image's Fourier transform. The Fourier 
transform of an atomic resolution image is similar, but different, to a 
diffraction pattern—with reciprocal lattice spots reflecting the symmetry and 
spacing of a crystal.

Does the above description mean that MicroED, or more broadly electron 
crystallogaphy does NOT suffer from phase problem?  How about single particle 
cryo electron microscopy, it should NOT have phase problem, right?

Thanks for any input in it.

Best,
Alex


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Re: [ccp4bb] polarizer

[Alexander Aleshin]

Ethan,
Thank you for describing existence of two types of polarizers. I use two 
crystal imagers/incubators, a smaller machine is easy to adjust to compensate 
for birefringence of plastic plates/covers, another is more capricious and the 
compensation is uneven over a plate. Company engineers explained it by using 
different types of polarizers, but I could not understand such a drastic 
difference in their behavior. Apparently, the second instrument uses the 
"quarter wave plate", right?

Alex

On 8/16/20, 12:40 PM, "CCP4 bulletin board on behalf of Ethan A Merritt" 
 wrote:

[EXTERNAL EMAIL]

On Sunday, 16 August 2020 12:14:59 PDT Diana Tomchick wrote:
> If only glass is placed between the polarizer and analyzer, the crystal 
will not show artificial colors (try it in a 9-well Pyrex depression plate). 
The artificial colors come from the diffraction of visible light from the 
plastic ware, which depending upon the type of plastic and the way the plate is 
manufactured, will have some preferred orientation of the polymer chains. 
Although it could have more to do with the method of manufacture of the plate.
>
> I would love to hear a different explanation from someone that either 
sells or manufactures crystallization plastic ware.

The question was about using a circular polarizer, which has two components,
a linear polarizing component and a quarter wave plate.

A pair of circular polarizers with a crystal between them will have the same
primary effect as a pair of linear polarizers.  But the "quarter wave 
plate" is
by its nature wavelength dependent.  So you get selective 
removal/transmission
of different color components.

Ethan


>
> Diana
>
> **
> Diana R. Tomchick
> Department of Biophysics, Rm. ND10.214A
> University of Texas Southwestern Medical Center
> 5323 Harry Hines Blvd.
> Dallas, TX 75061 USA
> 214-645-6383 (office)
>
> On Aug 16, 2020, at 12:19 PM, Nukri Sanishvili  wrote:
>
> 
> EXTERNAL MAIL
>
> Hi All,
>
> Adding some more details to what's been said already. Only because I've 
seen too many times the polarizers being used incorrectly.
> First, you need two polarization filters which are typically called 
polarizer and analyzer. First one (the polarizer) lets through only the light 
waves of a certain polarization. Then one needs to rotate the other one 
(analyzer) until there is no more light getting through. At this point the 
analyzer blocks the light that was let through by the polarizer This is what 
Diane referred to as 90 degrees. Please note that the polarizer-analyzer plates 
stay parallel to each other. After that, a crystal is placed between them and 
is rotated. Unless it is a crystal with cubic symmetry, at some angles it will 
light up in beautiful colors and at some angles it will not. This is because 
the crystal changes the polarization of the light passing through and "90 
degree setup" of the polarizer/analyzer pair is no longer valid for newly 
polarized light.
> Please note that using plastic plates in this context is not quite 
appropriate. The plastic polymer itself changes the polarization as well and 
therefore it breaks the main principle of this method. With plastic 
interference, it will be impossible to reach complete darkening of the field of 
view. I can almost hear a lot of people saying that they've used it with 
plastic plates without a problem. I believe it to be the case but it still 
doesn't make it right.
> Best,
> Nukri
>
> On Sun, Aug 16, 2020 at 9:15 AM Matthias Zeug 
mailto:matthias.z...@gmx.de>> wrote:
> Hi all,
>
> The polarizer-microscope in our facility is not working properly, and I 
have to check my plates using a standard stereo-microscope. As a workaround, I 
thought about buying one at Amazon, placing it on top of the plates and 
rotating it to still test for birefringence.
>
> The product is linked below. Does anyone have some experience with this 
kind of "homemade" system? And also (this might be a stupid question), does the 
product even work? As far as I know, the polarizers in the microscopes are 
linear polarizers, whereas the product linked below is a circular polarizer. I 
would also be happy for product recommendations (optimally available at the 
German Amazon).
>
> Cheers
>
> Matthias
>
> 
https://nam11.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.amazon.de%2Fdp%2FB00XNMXYBY%2Fref%3Dcm_sw_r_cp_apa_i_5YsoFbFQXTBP9&data=02%7C01%7Caaleshin%40SBPDISCOVERY.ORG%7C5ab3b2e3d3f74263048308d8421c30ef%7C0b162723004547deb0699f1a7aa955a1%7C0%7C1%7C637332036146230578&sdata=F62pskzH8aYInIOj45vHNt9IrHKM5NX0%2FHu1JZIwpTo%3D&reserved=0
>
> ___
> Buchmann Institute of Molecular Life Sciences
> Goethe University Frankfurt
> 
> 

Re: [ccp4bb] polarizer

[Ethan A Merritt]

On Sunday, 16 August 2020 12:14:59 PDT Diana Tomchick wrote:
> If only glass is placed between the polarizer and analyzer, the crystal will 
> not show artificial colors (try it in a 9-well Pyrex depression plate). The 
> artificial colors come from the diffraction of visible light from the plastic 
> ware, which depending upon the type of plastic and the way the plate is 
> manufactured, will have some preferred orientation of the polymer chains. 
> Although it could have more to do with the method of manufacture of the plate.
> 
> I would love to hear a different explanation from someone that either sells 
> or manufactures crystallization plastic ware.

The question was about using a circular polarizer, which has two components,
a linear polarizing component and a quarter wave plate.

A pair of circular polarizers with a crystal between them will have the same
primary effect as a pair of linear polarizers.  But the "quarter wave plate" is
by its nature wavelength dependent.  So you get selective removal/transmission
of different color components.

Ethan


> 
> Diana
> 
> **
> Diana R. Tomchick
> Department of Biophysics, Rm. ND10.214A
> University of Texas Southwestern Medical Center
> 5323 Harry Hines Blvd.
> Dallas, TX 75061 USA
> 214-645-6383 (office)
> 
> On Aug 16, 2020, at 12:19 PM, Nukri Sanishvili  wrote:
> 
> 
> EXTERNAL MAIL
> 
> Hi All,
> 
> Adding some more details to what's been said already. Only because I've seen 
> too many times the polarizers being used incorrectly.
> First, you need two polarization filters which are typically called polarizer 
> and analyzer. First one (the polarizer) lets through only the light waves of 
> a certain polarization. Then one needs to rotate the other one (analyzer) 
> until there is no more light getting through. At this point the analyzer 
> blocks the light that was let through by the polarizer This is what Diane 
> referred to as 90 degrees. Please note that the polarizer-analyzer plates 
> stay parallel to each other. After that, a crystal is placed between them and 
> is rotated. Unless it is a crystal with cubic symmetry, at some angles it 
> will light up in beautiful colors and at some angles it will not. This is 
> because the crystal changes the polarization of the light passing through and 
> "90 degree setup" of the polarizer/analyzer pair is no longer valid for newly 
> polarized light.
> Please note that using plastic plates in this context is not quite 
> appropriate. The plastic polymer itself changes the polarization as well and 
> therefore it breaks the main principle of this method. With plastic 
> interference, it will be impossible to reach complete darkening of the field 
> of view. I can almost hear a lot of people saying that they've used it with 
> plastic plates without a problem. I believe it to be the case but it still 
> doesn't make it right.
> Best,
> Nukri
> 
> On Sun, Aug 16, 2020 at 9:15 AM Matthias Zeug 
> mailto:matthias.z...@gmx.de>> wrote:
> Hi all,
> 
> The polarizer-microscope in our facility is not working properly, and I have 
> to check my plates using a standard stereo-microscope. As a workaround, I 
> thought about buying one at Amazon, placing it on top of the plates and 
> rotating it to still test for birefringence.
> 
> The product is linked below. Does anyone have some experience with this kind 
> of "homemade" system? And also (this might be a stupid question), does the 
> product even work? As far as I know, the polarizers in the microscopes are 
> linear polarizers, whereas the product linked below is a circular polarizer. 
> I would also be happy for product recommendations (optimally available at the 
> German Amazon).
> 
> Cheers
> 
> Matthias
> 
> https://www.amazon.de/dp/B00XNMXYBY/ref=cm_sw_r_cp_apa_i_5YsoFbFQXTBP9
> 
> ___
> Buchmann Institute of Molecular Life Sciences
> Goethe University Frankfurt
> 
> 
> 
> UT Southwestern
> 
> Medical Center
> 
> The future of medicine, today.
> 


-- 
Ethan A Merritt
Biomolecular Structure Center,  K-428 Health Sciences Bldg
MS 357742,   University of Washington, Seattle 98195-7742



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Re: [ccp4bb] polarizer

[Diana Tomchick]

If only glass is placed between the polarizer and analyzer, the crystal will 
not show artificial colors (try it in a 9-well Pyrex depression plate). The 
artificial colors come from the diffraction of visible light from the plastic 
ware, which depending upon the type of plastic and the way the plate is 
manufactured, will have some preferred orientation of the polymer chains. 
Although it could have more to do with the method of manufacture of the plate.

I would love to hear a different explanation from someone that either sells or 
manufactures crystallization plastic ware.

Diana

**
Diana R. Tomchick
Department of Biophysics, Rm. ND10.214A
University of Texas Southwestern Medical Center
5323 Harry Hines Blvd.
Dallas, TX 75061 USA
214-645-6383 (office)

On Aug 16, 2020, at 12:19 PM, Nukri Sanishvili  wrote:


EXTERNAL MAIL

Hi All,

Adding some more details to what's been said already. Only because I've seen 
too many times the polarizers being used incorrectly.
First, you need two polarization filters which are typically called polarizer 
and analyzer. First one (the polarizer) lets through only the light waves of a 
certain polarization. Then one needs to rotate the other one (analyzer) until 
there is no more light getting through. At this point the analyzer blocks the 
light that was let through by the polarizer This is what Diane referred to as 
90 degrees. Please note that the polarizer-analyzer plates stay parallel to 
each other. After that, a crystal is placed between them and is rotated. Unless 
it is a crystal with cubic symmetry, at some angles it will light up in 
beautiful colors and at some angles it will not. This is because the crystal 
changes the polarization of the light passing through and "90 degree setup" of 
the polarizer/analyzer pair is no longer valid for newly polarized light.
Please note that using plastic plates in this context is not quite appropriate. 
The plastic polymer itself changes the polarization as well and therefore it 
breaks the main principle of this method. With plastic interference, it will be 
impossible to reach complete darkening of the field of view. I can almost hear 
a lot of people saying that they've used it with plastic plates without a 
problem. I believe it to be the case but it still doesn't make it right.
Best,
Nukri

On Sun, Aug 16, 2020 at 9:15 AM Matthias Zeug 
mailto:matthias.z...@gmx.de>> wrote:
Hi all,

The polarizer-microscope in our facility is not working properly, and I have to 
check my plates using a standard stereo-microscope. As a workaround, I thought 
about buying one at Amazon, placing it on top of the plates and rotating it to 
still test for birefringence.

The product is linked below. Does anyone have some experience with this kind of 
"homemade" system? And also (this might be a stupid question), does the product 
even work? As far as I know, the polarizers in the microscopes are linear 
polarizers, whereas the product linked below is a circular polarizer. I would 
also be happy for product recommendations (optimally available at the German 
Amazon).

Cheers

Matthias

https://www.amazon.de/dp/B00XNMXYBY/ref=cm_sw_r_cp_apa_i_5YsoFbFQXTBP9

___
Buchmann Institute of Molecular Life Sciences
Goethe University Frankfurt



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The future of medicine, today.



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Re: [ccp4bb] polarizer

[Nukri Sanishvili]

Hi All,

Adding some more details to what's been said already. Only because I've
seen too many times the polarizers being used incorrectly.
First, you need two polarization filters which are typically called
polarizer and analyzer. First one (the polarizer) lets through only the
light waves of a certain polarization. Then one needs to rotate the other
one (analyzer) until there is no more light getting through. At this point
the analyzer blocks the light that was let through by the polarizer This is
what Diane referred to as 90 degrees. Please note that the
polarizer-analyzer plates stay parallel to each other. After that, a
crystal is placed between them and is rotated. Unless it is a crystal with
cubic symmetry, at some angles it will light up in beautiful colors and at
some angles it will not. This is because the crystal changes the
polarization of the light passing through and "90 degree setup" of the
polarizer/analyzer pair is no longer valid for newly polarized light.
Please note that using plastic plates in this context is not quite
appropriate. The plastic polymer itself changes the polarization as well
and therefore it breaks the main principle of this method. With plastic
interference, it will be impossible to reach complete darkening of the
field of view. I can almost hear a lot of people saying that they've used
it with plastic plates without a problem. I believe it to be the case but
it still doesn't make it right.
Best,
Nukri

On Sun, Aug 16, 2020 at 9:15 AM Matthias Zeug  wrote:

> Hi all,
>
>
>
> The polarizer-microscope in our facility is not working properly, and I
> have to check my plates using a standard stereo-microscope. As a
> workaround, I thought about buying one at Amazon, placing it on top of the
> plates and rotating it to still test for birefringence.
>
>
>
> The product is linked below. Does anyone have some experience with this
> kind of "homemade" system? And also (this might be a stupid question), does
> the product even work? As far as I know, the polarizers in the microscopes
> are linear polarizers, whereas the product linked below is a circular
> polarizer. I would also be happy for product recommendations (optimally
> available at the German Amazon).
>
>
>
> Cheers
>
>
>
> Matthias
>
>
>
> https://www.amazon.de/dp/B00XNMXYBY/ref=cm_sw_r_cp_apa_i_5YsoFbFQXTBP9
>
>
>
> ___
>
> Buchmann Institute of Molecular Life Sciences
>
> Goethe University Frankfurt
>
> --
>
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1
>



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Re: [ccp4bb] polarizer

[Boniecki, Michal]

You need to have crystal between polarizing plates. If you use only one on the 
top (close to your eye) and rotate filter you will “filter/polarize” all the 
light coming to your eye. What you need to buy is polarizing filter sheet 
(linear) which you place under your crystal and another one (the one that you 
submitted link) on the microscope (check size as you will have to secure it in 
order to rotate filter) The linear sheets can also be bought on amazon.

Michal

On Aug 16, 2020, at 09:47, V Nagarajan  wrote:


CAUTION: External to USask. Verify sender and use caution with links and 
attachments. Forward suspicious emails to phish...@usask.ca


Hi, place a linear polarizer below the tray and one above it, rotating the top 
one until you get the desired view.

V. Nagarajan

JANSi


On 8/16/2020 8:26 AM, Diana Tomchick wrote:
It's my understanding that you have two polarizers on your 
polarizer-microscope--one in the base, and the one that attaches to the 
magnifying lens. When you rotate the one on the lens so that it is 90 degrees 
to the one in the base, no (or very little) light should pass through to your 
eyes, unless there is a crystal that plane polarizes the light at an angle that 
differs from the two on the microscope.

What you have is just one polarizing lens. Not sure how that would work, even 
if it is a circular polarizer.

Diana

**
Diana R. Tomchick
Professor
Departments of Biophysics and Biochemistry
UT Southwestern Medical Center
5323 Harry Hines Blvd.
Rm. ND10.214A
Dallas, TX 75390-8816
diana.tomch...@utsouthwestern.edu
(214) 645-6383 (phone)
(214) 645-6353 (fax)

From: CCP4 bulletin board  
on behalf of Matthias Zeug 
Sent: Sunday, August 16, 2020 9:05 AM
To: CCP4BB@JISCMAIL.AC.UK 

Subject: [ccp4bb] polarizer


EXTERNAL MAIL

Hi all,



The polarizer-microscope in our facility is not working properly, and I have to 
check my plates using a standard stereo-microscope. As a workaround, I thought 
about buying one at Amazon, placing it on top of the plates and rotating it to 
still test for birefringence.



The product is linked below. Does anyone have some experience with this kind of 
"homemade" system? And also (this might be a stupid question), does the product 
even work? As far as I know, the polarizers in the microscopes are linear 
polarizers, whereas the product linked below is a circular polarizer. I would 
also be happy for product recommendations (optimally available at the German 
Amazon).



Cheers



Matthias



https://www.amazon.de/dp/B00XNMXYBY/ref=cm_sw_r_cp_apa_i_5YsoFbFQXTBP9



___

Buchmann Institute of Molecular Life Sciences

Goethe University Frankfurt



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The future of medicine, today.



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Re: [ccp4bb] polarizer

[V Nagarajan]

Hi, place a linear polarizer below the tray and one above it, rotating 
the top one until you get the desired view.


V. Nagarajan

JANSi


On 8/16/2020 8:26 AM, Diana Tomchick wrote:
It's my understanding that you have two polarizers on your 
polarizer-microscope--one in the base, and the one that attaches to 
the magnifying lens. When you rotate the one on the lens so that it is 
90 degrees to the one in the base, no (or very little) light should 
pass through to your eyes, unless there is a crystal that plane 
polarizes the light at an angle that differs from the two on the 
microscope.


What you have is just one polarizing lens. Not sure how that would 
work, even if it is a circular polarizer.


Diana

**
Diana R. Tomchick
Professor
Departments of Biophysics and Biochemistry
UT Southwestern Medical Center
5323 Harry Hines Blvd.
Rm. ND10.214A
Dallas, TX 75390-8816
diana.tomch...@utsouthwestern.edu
(214) 645-6383 (phone)
(214) 645-6353 (fax)

*From:* CCP4 bulletin board  on behalf of 
Matthias Zeug 

*Sent:* Sunday, August 16, 2020 9:05 AM
*To:* CCP4BB@JISCMAIL.AC.UK 
*Subject:* [ccp4bb] polarizer

EXTERNAL MAIL

Hi all,

The polarizer-microscope in our facility is not working properly, and 
I have to check my plates using a standard stereo-microscope. As a 
workaround, I thought about buying one at Amazon, placing it on top of 
the plates and rotating it to still test for birefringence.


The product is linked below. Does anyone have some experience with 
this kind of "homemade" system? And also (this might be a stupid 
question), does the product even work? As far as I know, the 
polarizers in the microscopes are linear polarizers, whereas the 
product linked below is a circular polarizer. I would also be happy 
for product recommendations (optimally available at the German Amazon).


Cheers

Matthias

https://www.amazon.de/dp/B00XNMXYBY/ref=cm_sw_r_cp_apa_i_5YsoFbFQXTBP9

___

Buchmann Institute of Molecular Life Sciences

Goethe University Frankfurt




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Medical Center


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Re: [ccp4bb] polarizer

[Diana Tomchick]

It's my understanding that you have two polarizers on your 
polarizer-microscope--one in the base, and the one that attaches to the 
magnifying lens. When you rotate the one on the lens so that it is 90 degrees 
to the one in the base, no (or very little) light should pass through to your 
eyes, unless there is a crystal that plane polarizes the light at an angle that 
differs from the two on the microscope.

What you have is just one polarizing lens. Not sure how that would work, even 
if it is a circular polarizer.

Diana

**
Diana R. Tomchick
Professor
Departments of Biophysics and Biochemistry
UT Southwestern Medical Center
5323 Harry Hines Blvd.
Rm. ND10.214A
Dallas, TX 75390-8816
diana.tomch...@utsouthwestern.edu
(214) 645-6383 (phone)
(214) 645-6353 (fax)

From: CCP4 bulletin board  on behalf of Matthias Zeug 

Sent: Sunday, August 16, 2020 9:05 AM
To: CCP4BB@JISCMAIL.AC.UK 
Subject: [ccp4bb] polarizer


EXTERNAL MAIL

Hi all,



The polarizer-microscope in our facility is not working properly, and I have to 
check my plates using a standard stereo-microscope. As a workaround, I thought 
about buying one at Amazon, placing it on top of the plates and rotating it to 
still test for birefringence.



The product is linked below. Does anyone have some experience with this kind of 
"homemade" system? And also (this might be a stupid question), does the product 
even work? As far as I know, the polarizers in the microscopes are linear 
polarizers, whereas the product linked below is a circular polarizer. I would 
also be happy for product recommendations (optimally available at the German 
Amazon).



Cheers



Matthias



https://www.amazon.de/dp/B00XNMXYBY/ref=cm_sw_r_cp_apa_i_5YsoFbFQXTBP9



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Goethe University Frankfurt



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[ccp4bb] polarizer

[Matthias Zeug]

Hi all,



The polarizer-microscope in our facility is not working properly, and I have
to check my plates using a standard stereo-microscope. As a workaround, I
thought about buying one at Amazon, placing it on top of the plates and
rotating it to still test for birefringence.



The product is linked below. Does anyone have some experience with this kind
of "homemade" system? And also (this might be a stupid question), does the
product even work? As far as I know, the polarizers in the microscopes are
linear polarizers, whereas the product linked below is a circular polarizer.
I would also be happy for product recommendations (optimally available at
the German Amazon).



Cheers



Matthias



https://www.amazon.de/dp/B00XNMXYBY/ref=cm_sw_r_cp_apa_i_5YsoFbFQXTBP9



___

Buchmann Institute of Molecular Life Sciences

Goethe University Frankfurt




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[ccp4bb] ADP-Glo assay vs Kinase Glo assay? Or an assay for weak kinase activity?

[Saif Mohd]

Greetings everyone,
I am looking for an assay suitable to detect atleast, 500nM to 1mM ATP
hydrolysis. My Kinase has a very weak activity. The signal should be good
enough (also must be duplicated) to have good signal/noise and Z´values.
I want to use the assay for Inhibitor screening. Then the goal is to use
the compound in cell-based assays and do structural studies.
ADP-Glo assay is pretty expensive.Cost factor comes into play as I want to
screen atleast 5K compounds.
In the same cost of 1000 rxns of ADP-Glo assay I can do 10,000 rxns of
Kinase Glo assay.
Does anyone have experience with Kinase Glo plus assay (linear upto 100uM
ATP)? and could please recommend its suitability.
Or alternatively any other suitable assay for the above mentioned
requirement.
PS- Malachite green assay (MLG) is not suitable.
With another kinase, I used MLG assay and screened 16K compounds. MLG dye,
I made myself and it is cheap.
Thanks and best wishes,
Have a nice Sunday,
Saif



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