Re: [ccp4bb] Fluka-branded PEG 3000

[David Briggs]

Hi Galen,

Is it possibly the age of the original material, rather than the batch or 
brand? PEG does oxidise over time.

Best,

Dave

--
Dr David C. Briggs
Senior Laboratory Research Scientist
Signalling and Structural Biology Lab
The Francis Crick Institute
London, UK
==
about.me/david_briggs


From: CCP4 bulletin board  on behalf of Correy, Galen 

Sent: Monday, March 22, 2021, 17:10
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Fluka-branded PEG 3000


External Sender: Use caution.

Dear all,

I'm wondering if anyone could spare 50-100 g of Fluka branded PEG 3000 (see 
attached image)?

We have some crystals that diffract better when grown using Fluka PEG compared 
to other brands. Unfortunately, we've exhausted our supply of the Fluka PEG, 
and it's no longer commercially available (the same product code is available 
from Sigma - we've tested it, along with several other brands - but they don't 
seem to have the same magic as the Fluka PEG).

We'll happily purchase a new bottle of Sigma-branded PEG 3000 to replace any 
sent our way.

Many thanks,
Galen



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Re: [ccp4bb] unknown density

[Pearce, N.M. (Nick)]

This doesn’t surprise me in the slightest, given what how the two maps are 
calculated.

The polder map is essentially the same as your "simple omit map” without the 
bulk solvent contribution that is present in the omit-refine map. Since this is 
an approximately constant contribution, if you recontour the Polder map to a 
higher contour you should be able to get the two maps to look basically the 
same.

Nick

On 23 Mar 2021, at 09:36, Sam Tang 
mailto:samtys0...@gmail.com>> wrote:

Dear colleagues

Thanks a lot for the comments. I echo that this pentagon-like density may 
likely be water molecules. What had puzzled us a bit was that the output of 
Polder map which gave some interlinked density.

I should also mention that this site is close to a predicted peptide binding 
site and that is what we are actually looking for. (To me the strong positive 
density in Polder map is not indicative of an amino acid residue as well.)

My Polder map looks like this:
https://drive.google.com/file/d/10dGpdcDhrp_ld6wPH3Er7iZ3KVgU2RCN/view?usp=sharing

BRS

Sam

On Tue, 23 Mar 2021 at 04:43, Bernhard Rupp 
mailto:hofkristall...@gmail.com>> wrote:
Textbook knowledge 😊
http://www.ruppweb.org/Garland/gallery/Ch12/pages/Biomolecular_Crystallography_Fig_12-28.htm

--
Bernhard Rupp
http://www.hofkristallamt.org/
b...@hofkristallamt.org
+1 925 209 7429
+43 676 571 0536
-
Doors and corners – that’s where they get you
-





From: CCP4 bulletin board mailto:CCP4BB@JISCMAIL.AC.UK>> 
On Behalf Of Sharan Karade
Sent: Monday, March 22, 2021 13:10
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] unknown density

Hi

Please look at the pic attached. found similar pentagon water network.



On Mon, Mar 22, 2021 at 12:40 PM Jon Cooper 
<488a26d62010-dmarc-requ...@jiscmail.ac.uk>
 wrote:
Definitely water pentamer, no doubt at all ;-0
Cheers, Jon.C.

Sent from ProtonMail mobile



 Original Message 
On 22 Mar 2021, 14:16, Mark J. van Raaij < 
mjvanra...@cnb.csic.es> wrote:

The ring looks too big to be imidazole or a nucleotide or a carbohydrate, so 
it’s probably mainly water molecules.
Perhaps partially replaced by PEG to explain the density between them (i.e. 
water molecules in most copies of the protein and PEG in some other copies). 
I’ve seen horse-shoe shaped PEG in a high-res structure before, PEGs in several 
confirmations might explain a circle.
Practically speaking, I’d first model five waters and see if they refine well.

Mark


On 22 Mar 2021, at 14:58, Sam Tang 
mailto:samtys0...@gmail.com>> wrote:

Hello fellow colleagues

Hope you are all well while the pandemics persists. I just wonder if anyone may 
have an idea what this density (looking like a pentagon) might be. The data was 
collected to 1.8 A and crystal was grown in Bis-tris + PEG3350. Imidazole 
residual? Nucleotide (the protein itself is nucleotide-binding, but shouldn't 
be at this particular site)?

https://drive.google.com/file/d/1L9UBFmW72P214itM2HJR_DVy3FaA6FEZ/view?usp=sharing

Thanks!

BRS

Sam



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--
Sharan Karade
Postdoc-fellow
IBBR-UMD, 9600 Gudelsky Dr,
Rockville
Maryland 20850




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Re: [ccp4bb] unknown density

[Barone, Matthias]

can confirm jon´s comment. I find these in virtually every high-res structure. 
some of them wobble a bit given the AA close by, such as Arg.


Dr. Matthias Barone

AG Kuehne, Rational Drug Design

Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP)
Robert-Rössle-Strasse 10
13125 Berlin

Germany
Phone: +49 (0)30 94793-284


From: CCP4 bulletin board  on behalf of Jon Cooper 
<488a26d62010-dmarc-requ...@jiscmail.ac.uk>
Sent: Monday, March 22, 2021 5:38:40 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] unknown density

Definitely water pentamer, no doubt at all ;-0
Cheers, Jon.C.

Sent from ProtonMail mobile



 Original Message 
On 22 Mar 2021, 14:16, Mark J. van Raaij < mjvanra...@cnb.csic.es> wrote:

The ring looks too big to be imidazole or a nucleotide or a carbohydrate, so 
it’s probably mainly water molecules.
Perhaps partially replaced by PEG to explain the density between them (i.e. 
water molecules in most copies of the protein and PEG in some other copies). 
I’ve seen horse-shoe shaped PEG in a high-res structure before, PEGs in several 
confirmations might explain a circle.
Practically speaking, I’d first model five waters and see if they refine well.

Mark

On 22 Mar 2021, at 14:58, Sam Tang 
mailto:samtys0...@gmail.com>> wrote:

Hello fellow colleagues

Hope you are all well while the pandemics persists. I just wonder if anyone may 
have an idea what this density (looking like a pentagon) might be. The data was 
collected to 1.8 A and crystal was grown in Bis-tris + PEG3350. Imidazole 
residual? Nucleotide (the protein itself is nucleotide-binding, but shouldn't 
be at this particular site)?

https://drive.google.com/file/d/1L9UBFmW72P214itM2HJR_DVy3FaA6FEZ/view?usp=sharing

Thanks!

BRS

Sam



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[ccp4bb] MOSBRI: A new TNA for Molecular-Scale Biophysics Research Infrastructure

[Margret Fischer]

Dear all,

We would like to share with you the press release of the recently EU 
granted MOSBRI (Molecular-Scale Biophysics Research Infrastructure ). 
MOSBRI will connect leading molecular biophysics facilities across 11 
countries, including 13 academic centres of excellence and 2 industrial 
partners. Under the coordination of Institut Pasteur in France, the 
consortium will make their services available across the continent to 
scientists from both academia and industry. Each facility will 
contribute cutting-edge instrumentation and unique expertise. MOSBRI’s 
portfolio of services will span the entire field of molecular-scale 
biophysics.


Access to the large panel of cutting-edge biophysical technologies and 
unparalleled range of expertise of the consortium will be available at 
no cost to all European researchers from both academia and industry. 
MOSBRI will commence operations on the 1st of July of 2021.


For more information visit our website (https://www.mosbri.eu 
) or email cont...@mosbri.eu


Best,
Maria Garcia Alai

(on behalf of the MOSBRI consortium)

--

Maria Garcia Alai, PhD

Team Leader,
Head of Facility
Sample Preparation and Characterization
& High-throughput Crystallization

EMBL Hamburg Outstation
Address: EMBL (c/o DESY)
Notkestrasse 85, 22607
Hamburg, Germany
T +49 40 89902145 | F +49 40 89902149
www.embl-hamburg.de





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[ccp4bb] E.coli cell lysis using glass beads

[Dr. Veerendra Kumar]

Dear All,
Sorry for the off topic.
May I ask your experience about bacterial cell lysis using glass beads beating? 
How is the cell lysis compared with sonication and French press? Does the glass 
beads affect the protein quality?

Thank you in advance.

Best Regards
Veerendra Kumar, PhD
Associate Professor & Ramalingaswami Fellow
Amity Institute of Molecular Medicine & Stem Cell Research (AIMMSCR)
J-3 108, Amity University Campus
Sector-125, Noida-201303
UP, INDIA
http://amity.edu/aimmscr/Faculty.asp
Google Scholar: https://scholar.google.com/citations?user=ymdnsUAJ&hl=en




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[ccp4bb] [just for fun] please vote #JBCMethodsMadness #TeamXRC

[Javier Gonzalez]

just one hour left !!
https://twitter.com/jbiolchem/status/1374030429729271811?s=20

-- 
Dr. Javier M. González
Instituto de Bionanotecnología del NOA (INBIONATEC-CONICET)
Universidad Nacional de Santiago del Estero (UNSE)
RN9, Km 1125. Villa El Zanjón. (G4206XCP)
Santiago del Estero. Argentina
Tel: +54-(0385)-4238352



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Re: [ccp4bb] Fluka-branded PEG 3000

[Wiener, Michael C (mcw2s)]

During a prior period of great concern about variable peroxidation levels of 
commercial PEGs (and of polyoxyethylene detergents), we measured/monitored 
peroxide levels of solutions. The Pierce kit is now available from Fisher 
https://www.fishersci.com/shop/products/pierce-quantitative-peroxide-assay-kit-aqueous/PI23280#?keyword=Peroxide%20Assay%20Kit
 or you could DIY if you like. This was, of course, in addition to making small 
amounts of stock solutions + using brown glass (not plastic) for storage.

Good luck with your bedeviling issue.

Regards,

-MW

--
Michael C. Wiener, Ph.D.
Professor of Molecular Physiology and Biological Physics
University of Virginia
PO Box 800886
Charlottesville, VA 22908-0886
434-243-2731 (office)
434-243-2730 (lab)

From: CCP4 bulletin board  on behalf of David Briggs 

Reply-To: David Briggs 
Date: Tuesday, March 23, 2021 at 4:20 AM
To: "CCP4BB@JISCMAIL.AC.UK" 
Subject: Re: [ccp4bb] Fluka-branded PEG 3000

Hi Galen,

Is it possibly the age of the original material, rather than the batch or 
brand? PEG does oxidise over time.

Best,

Dave

--
Dr David C. Briggs
Senior Laboratory Research Scientist
Signalling and Structural Biology Lab
The Francis Crick Institute
London, UK
==
about.me/david_briggs


From: CCP4 bulletin board  on behalf of Correy, Galen 

Sent: Monday, March 22, 2021, 17:10
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Fluka-branded PEG 3000


External Sender: Use caution.

Dear all,

I'm wondering if anyone could spare 50-100 g of Fluka branded PEG 3000 (see 
attached image)?

We have some crystals that diffract better when grown using Fluka PEG compared 
to other brands. Unfortunately, we've exhausted our supply of the Fluka PEG, 
and it's no longer commercially available (the same product code is available 
from Sigma - we've tested it, along with several other brands - but they don't 
seem to have the same magic as the Fluka PEG).

We'll happily purchase a new bottle of Sigma-branded PEG 3000 to replace any 
sent our way.

Many thanks,
Galen



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with its registered office at 1 Midland Road London NW1 1AT



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Re: [ccp4bb] Suggestions to improve resolution of protein crystals

[Ashish Kumar]

Hi Saurabh,

I have also encountered a similar problem. Sometimes, though your protein
looks pure in size exclusion chromatography, but actually is not very pure
and may contain traces of partially unfolded form or some transient
oligomeric forms. In such a case, your size exclusion profile would be very
broad and if such impurities remain within, it would affect the packing of
crystal resulting in poor diffraction.

What I have done to get rid of it is, I tried mild heating at 42 degrees C
for 5 min, spin down the protein at very high speed, take out the
supernatant and straight away go for crystallization. If by any chance your
protein has some thermal stability, you could also, try mild heating before
crystallization. This removes the trace contaminants and makes your sample
more homogenous.

Alternatively, if your protein sequence has long disorder regions
internally, then you could try in-situ proteolysis also. Using a protease
at a very low concentration in your crystallization drop would chop off the
disordered loops in your protein, which would favor a better packing.

Also, you can try slightly altering the length of your construct, wherein
if the ends are disordered or highly charged, you can trim those regions
from your construct to get a more stable and homogenous sample.

Another thing I noticed is your two conditions have PEG in them. For those
conditions sometimes a low concentration (20-25%) of  PEG 400 works best as
a cryoprotectant. You could try using that as well.

I wish you all the best with your trials.

Best
Ashish Kumar

On Sun, Mar 21, 2021 at 11:04 AM Saurabh Upadhyay 
wrote:

> Dear All,
>
> I am trying to crystalize a protein, which occurs in monomeric (60kDa) and
> tetrameric (240kDa) form. *The protein during crystallization was in
> MES buffer pH-6 *and the method of crystallization was "*Sitting drop*"
> in "*Proplex*" condition. Some of the conditions in which crystals were
> obtained are:
>
> *1) 0.1M HEPES pH-7; 10% (w/v) PEG 4000*
> *2) 0.1M Tris, pH-8; 8% (w/v) PEG 8000*
> *3) 0.1M Tris, pH-8; 25% (v/v) PEG 400*
>
> For reference, I have attached the images of the crystals observed below.
>
> *However, the diffraction pattern obtained and the resolution of crystals
> were very poor. Even some mosaicity has been observed.  *
>
> Kindly suggest some methods or modifications, to improve the resolution
> and  diffraction pattern of the crystals obtained.
>
> Thanking You,
> Sincerely,
> Saurabh Upadhyay,
> Ph.D. Scholar
> c/o Dr. Ashok kumar Patel,
> Kusuma School of Biological Sciences,
> Indian Institute of Technology, Hauz Khas,
> New Delhi-110016, India
>
>
> --
>
> To unsubscribe from the CCP4BB list, click the following link:
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>



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Re: [ccp4bb] Fluka-branded PEG 3000

[Correy, Galen]

Dear all,

Thank you for the kind offers of PEG, and for the helpful suggestions.

I'm hoping a return to Fluka-branded PEG will solve my issues, but if it 
doesn't, I'll be sure to follow up on some of the other suggestions.

Thanks again,
Galen


From: CCP4 bulletin board  on behalf of Wiener, Michael 
C (mcw2s) 
Sent: Tuesday, 23 March 2021 8:56 AM
To: CCP4BB@JISCMAIL.AC.UK 
Subject: Re: [ccp4bb] Fluka-branded PEG 3000


During a prior period of great concern about variable peroxidation levels of 
commercial PEGs (and of polyoxyethylene detergents), we measured/monitored 
peroxide levels of solutions. The Pierce kit is now available from Fisher 
https://www.fishersci.com/shop/products/pierce-quantitative-peroxide-assay-kit-aqueous/PI23280#?keyword=Peroxide%20Assay%20Kit
 or you could DIY if you like. This was, of course, in addition to making small 
amounts of stock solutions + using brown glass (not plastic) for storage.



Good luck with your bedeviling issue.



Regards,



-MW



--

Michael C. Wiener, Ph.D.

Professor of Molecular Physiology and Biological Physics

University of Virginia

PO Box 800886

Charlottesville, VA 22908-0886

434-243-2731 (office)

434-243-2730 (lab)



From: CCP4 bulletin board  on behalf of David Briggs 

Reply-To: David Briggs 
Date: Tuesday, March 23, 2021 at 4:20 AM
To: "CCP4BB@JISCMAIL.AC.UK" 
Subject: Re: [ccp4bb] Fluka-branded PEG 3000



Hi Galen,



Is it possibly the age of the original material, rather than the batch or 
brand? PEG does oxidise over time.



Best,



Dave



--
Dr David C. Briggs
Senior Laboratory Research Scientist
Signalling and Structural Biology Lab
The Francis Crick Institute
London, UK
==
about.me/david_briggs





From: CCP4 bulletin board  on behalf of Correy, Galen 

Sent: Monday, March 22, 2021, 17:10
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Fluka-branded PEG 3000



External Sender: Use caution.



Dear all,



I'm wondering if anyone could spare 50-100 g of Fluka branded PEG 3000 (see 
attached image)?



We have some crystals that diffract better when grown using Fluka PEG compared 
to other brands. Unfortunately, we've exhausted our supply of the Fluka PEG, 
and it's no longer commercially available (the same product code is available 
from Sigma - we've tested it, along with several other brands - but they don't 
seem to have the same magic as the Fluka PEG).



We'll happily purchase a new bottle of Sigma-branded PEG 3000 to replace any 
sent our way.



Many thanks,

Galen





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https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1



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Wales no. 1140062 and a company registered in England and Wales no. 06885462, 
with its registered office at 1 Midland Road London NW1 1AT





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[ccp4bb] academic users of XDS: time to update the old version

[Kay Diederichs]

Dear academic users of XDS,

the latest version of XDS has its release notes now at https://xds.mr.mpg.de 
(the old xds.mpimf-heidelberg.de can still be used but is redirected).
The download location can also be found there.

If your current (academic license) version of XDS is from last year, then it 
will expire in one week. So it's time to update!

best wishes,
Kay



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Re: [ccp4bb] academic users of XDS: time to update the old version

[Kay Diederichs]

Sorry, I mistyped the old XDS site address. Correct is 
http://xds.mpimf-heidelberg.mpg.de/ . 
best,
Kay


On Tue, 23 Mar 2021 17:12:33 +, Kay Diederichs 
 wrote:

>Dear academic users of XDS,
>
>the latest version of XDS has its release notes now at https://xds.mr.mpg.de 
>(the old xds.mpimf-heidelberg.de can still be used but is redirected).
>The download location can also be found there.
>
>If your current (academic license) version of XDS is from last year, then it 
>will expire in one week. So it's time to update!
>
>best wishes,
>Kay
>
>
>
>To unsubscribe from the CCP4BB list, click the following link:
>https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1
>
>This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing 
>list hosted by www.jiscmail.ac.uk, terms & conditions are available at 
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[ccp4bb] Two positions available at UAMS

[Enemark, Eric J]

Hi,

A postdoctoral position and a research staff position are available in the 
Enemark lab for highly motivated applicants to study the structure and function 
of helicase proteins and their mechanism of action on nucleic acids.  For 
representative publications, see:

M. Meagher*, L. B. Epling*, E. J. Enemark, "DNA translocation mechanism of the 
MCM complex and implications for replication initiation", Nature Communications 
(2019).
J. M. Miller*, B. T. Arachea*, L. B. Epling, E. J. Enemark, "Analysis of the 
crystal structure of an active MCM hexamer," Elife 3:e03433 (2014).
C. A. Froelich*, S. Kang*, L. B. Epling, S. P. Bell#, E. J. Enemark#, "A 
conserved MCM single-stranded DNA binding element is essential for replication 
initiation," Elife 3: e01993 (2014).

Applicants should have recent PhD experience in biochemistry, crystallography, 
cryoEM, or a related field and possess a strong interest in structural biology. 
 The laboratory is located in the Department of Biochemistry and Molecular 
Biology at the University of Arkansas for Medical Sciences.  The department is 
highly interactive with extensive expertise and interest in fundamental nucleic 
acids processes.  Diverse interactions occur on campus within the Winthrop P. 
Rockefeller Cancer Institute (https://cancer.uams.edu/).  The Winthrop P. 
Rockefeller Cancer Institute has made sizable investments to strengthen 
structural biology at UAMS and to make it an integral part of its cancer 
research programs.

To apply, please visit the UAMS employment website addresses below or email a 
cover letter, CV with list of publications, and a list of 3 references to: 
ejenem...@uams.edu

https://external-uams.icims.com/jobs/73188/post-doctoral-fellow/job?hub=6
https://external-uams.icims.com/jobs/73187/research-associate-iii/job?hub=6

Best,
Eric

Eric J. Enemark
Associate Professor
University of Arkansas for Medical Sciences
Department of Biochemistry and Molecular Biology
4301 W. Markham St., slot 516
Little Rock, AR 72205
Email: ejenem...@uams.edu

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[ccp4bb] March 24, 2021 - CBMS Lecture Series - Self-Assembling DNA crystal Scafflolds for Arrangement of Biomaterials

[Stojanoff, Vivian]

Exploring Self-Assembling DNA Crystal Scaffolds for the Precise Arrangement of 
Biomaterials

Tara MacCulloch, Arizona State University

 Center for Molecular Design and Biomimetics at the Biodesign Institute



Wednesday March 24; 1:30 pm



LINK:  
https://bnl.zoomgov.com/meeting/register/vJItf-2grT4qHCtWowgMeA6RqJtpusmuG-k

as a confirmation of your registration, you will receive the link to the 
lecture.

Abstract: The foundational goal of structural DNA nanotechnology is to create 
rationally designed crystal lattices to precisely organize macromolecules 
untenable for crystallization to potentially result in the structure of the 
guest species with X-ray crystallography. DNA is ideal for the construction of 
three-dimensional crystals due to its distinctive ability to associate via 
canonical Watson-Crick base pairing which can anneal into a series of “Holliday 
junctions” that are tailed by complementary “sticky ends” which cohere to form 
the 3D arrays. Using these features, the structures of only a handful of these 
DNA scaffolds have been determined. We have recently determined the 2.7 Å 
structure of a prescribed rhombohedral (R3) lattice containing atomic detail 
not previously achieved in any other self-assembled DNA crystal system. The 
role of sticky end sequence was also exhaustively explored to probe its role in 
crystal order, resulting in a related 2.6 Å scaffold. Additionally, we have 
undertaken a comprehensive study of the imperative role that sequence plays at 
each 4-arm junction crossover within the lattice to dictate symmetry and 
resolution, and to allow for the site-specific placement of guest molecules 
with atomic precision. These systems provide significant promise towards the 
construction of improved 3D DNA lattices which could be used as frameworks for 
immobilizing and solving the structure of molecular guests, templating 
catalytic materials, and yield significant insight into the mechanism of DNA 
self-assembly.






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Re: [ccp4bb] E.coli cell lysis using glass beads

[Dr. Veerendra Kumar]

Thank you Prof Raaij.
I also have bad experience with sonication for one proteins. It denatures after 
lysis with sonicator (1sec on/off pulse, 30% amp, 5 min). However, when we lyse 
the cell with beads (vortexing with beads for 1 min, 5 times), we were able to 
get good protein.

Best Regards

Veerendra Kumar, PhD
Associate Professor & Ramalingaswami Fellow
Amity Institute of Molecular Medicine & Stem Cell Research (AIMMSCR)
J-3 108, Amity University Campus
Sector-125, Noida-201303
UP, INDIA
http://amity.edu/aimmscr/Faculty.asp
Google Scholar: https://scholar.google.com/citations?user=ymdnsUAJ&hl=en

From: Mark J. van Raaij 
Sent: 23 March 2021 18:13
To: Dr. Veerendra Kumar 
Subject: Re: [ccp4bb] E.coli cell lysis using glass beads

In my opinion it is better than sonication, but more work, especially if you 
want to wash the beads and recycle them. So I haven’t been able to convince 
people in the lab to use it more than a few times.
French press could still be better or about the same, not sure.
I also think it should affect protein quality less than sonication, perhaps 
about the same as French press.

Mark J van Raaij
Dpto de Estructura de Macromoleculas
Centro Nacional de Biotecnologia - CSIC
calle Darwin 3
E-28049 Madrid, Spain
Section Editor Acta Crystallographica F
https://journals.iucr.org/f/


On 23 Mar 2021, at 13:23, Dr. Veerendra Kumar 
mailto:vkuma...@amity.edu>> wrote:

Dear All,
Sorry for the off topic.
May I ask your experience about bacterial cell lysis using glass beads beating? 
How is the cell lysis compared with sonication and French press? Does the glass 
beads affect the protein quality?

Thank you in advance.

Best Regards
Veerendra Kumar, PhD
Associate Professor & Ramalingaswami Fellow
Amity Institute of Molecular Medicine & Stem Cell Research (AIMMSCR)
J-3 108, Amity University Campus
Sector-125, Noida-201303
UP, INDIA
http://amity.edu/aimmscr/Faculty.asp
Google Scholar: https://scholar.google.com/citations?user=ymdnsUAJ&hl=en



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