[ccp4bb] off-topic: protein losing FAD during purification
Dear All (those dealing with wetlab stuff..), While purifying a FAD containing protein we lose part of the FAD (on the gel filtration we clearly see two bands corresponding to holoprotein and free FAD). We obtain crystals but diffracting to only about 4 A despite their beautiful look. Our hypothesis is that the crystals contain a population of molecules with and without FAD (?). The questions are: 1) how to keep FAD bound to the protein during purification and crystallization? 2) how to completely remove FAD from the protein? Thank you very much for any help provided! Best regards Stefano (part-time wetlab person) Dr Stefano Benini, Ph.D. Assistant Professor First International workshop: Molecular Basis of Fire Blight, Bolzano 15.10.2014 Laboratory homepage: http://pro.unibz.it/staff2/sbenini/B2Cl.htm Personal homepage http://pro.unibz.it/staff2/sbenini/ I don't like anything that's fake and I hate pretenders! * Bioorganic chemistry and Bio-Crystallography laboratory (B2Cl) Faculty of Science and Technology Free University of Bolzano Piazza Università, 5 39100 Bolzano, Italy Office (room K2.14): +39 0471 017128 Laboratory (room E.021): +39 0471 017910 Fax: +39 0471 017009 ogni giorno in più è un giorno in meno.
Re: [ccp4bb] video of crystal pick-up through microscope?
And when handling liquid nitrogen don't forget to get protections against frost bite (goggles, cryogloves) S. -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Swastik Phulera Sent: Friday, 13 September, 2013 15:08 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] video of crystal pick-up through microscope? Hi, May be this would help https://www.youtube.com/watch?v=QcsaWowulDM On Fri, Sep 13, 2013 at 2:51 PM, Frank von Delft frank.vonde...@sgc.ox.ac.uk wrote: Hi all - does anybody know where I can find a video of a crystal being harvested, but taken through a microscope? The Google has not been helpful, but I'm obviouly incompetent with these new-fangled things. Thanks! phx -- -- स्वस्तिक फुलेरा वरिष्ठ अनुसंधान कर्ता संरचनात्मक जीवविज्ञान प्रयोगशाला डी. एन. ए. फिंगरप्रिंटिंग एवं निदान केंद्र हैदराबाद, ५००, ००१ और राष्ट्रीय कोशिका विज्ञान केंद्र पुणे विश्वविद्यालय परिसर, गणेशखिंड पुना, ४११,००७
[ccp4bb] PhD program at the Free University of Bolzano (Italy)
Dear All, a new call for applicants for a PhD fellowship at the Free University of Bolzano is now out. Please follow the link http://www.unibz.it/en/public/research/phd/Documents/bando_dottorati_29esimo_ciclo_en.pdf A suitable candidate will be selected for a position in my group at the Faculty of Science and Technology PhD programme in MOUNTAIN ENVIRONMENTS AND AGRICULTURE Website: http://www.unibz.it/en/sciencetechnology/progs/phd/phdmountainenvironment/default.html The following two projects are available for the selected candidate 1)Biomolecular characterization of proteins from Erwinia amylovora's amylovoran biosynthetic pathway 2)Biomolecular characterization of proteins from Erwinia amylovora's bacteriophages. How to fight fire blight using its natural killer. candidate interested are welcome to contact me for information bust MUST apply following the rules and links as specified in the call other links http://www.unibz.it/en/public/research/phd/prospectivePhdstudents.html pre-enrole here: https://aws.unibz.it/exup/en/Account/Logon?ReturnUrl=%2fexup%2fen%2fHome%2fIndex Please note that Bolzano is located near the UNESCO site of the Dolomites with some of the best hiking paths and slopes for skiing. http://whc.unesco.org/en/list/1237/ http://www.obereggen.com/en/winter_activities/ with my best regards Dr Stefano Benini, Ph.D. Assistant Professor http://pro.unibz.it/staff2/sbenini/ I don't like anything that's fake and I hate pretenders! Sir Arthur Thispairof * Bioorganic chemistry and Bio-Crystallography laboratory (B2Cl) Faculty of Science and Technology Free University of Bolzano Piazza Università, 5 39100 Bolzano, Italy Office (room K2.14): +39 0471 017128 Laboratory (room E.021): +39 0471 017910 Fax: +39 0471 017009
[ccp4bb] Reminder: PhD position in structural biology at the Free University of Bolzano
Dear All (possible candidates!), A 3 year PhD fellowship is available to a suitable candidate for one of the following projects involving molecular biology and structural biology techniques: Molecular characterization of the bio synthetic pathway of amylovoran, a pathogenicity factor in Erwinia amylovora Biomolecular study of iron uptake by desferrioxamine in the plant pathogen Erwinia amylovora the work will involve cloning of genes, protein expression, purification and crystallization and structure determination by X-ray crystallography. Proteins will be characterized using in solution techniques including NMR available through collaboration with other laboratories. To apply to the public competition please visit the link: http://www.unibz.it/en/public/research/phd/prospectivePhdstudents.html there you will find the link to the official call document: http://www.unibz.it/SiteCollectionDocuments/2012_PhD_Ausschreibung_en.pdf as well as the link to the pre-enrolment page: https://aws.unibz.it/exup/ The applications must be sent by 31 August 2012. It is recommended that applications are completed and sent as soon as possible If you wish to apply and want more information on the project and for informal enquiries please contact: stefano.ben...@unibz.it For information on how to apply please contact our secretary: stefania.falc...@unibz.it Best regards Stefano Benini, Ph.D. Assistant Professor http://pro.unibz.it/staff2/sbenini/ * Laboratory of Bioorganic Chemistry and Crystallography Faculty of Science and Technology Free University of Bolzano Piazza Università, 5 39100 Bolzano, Italy Office (room K2.14): +39 0471 017128 Laboratory (room E.012): +39 0471 017901 Fax: +39 0471 017009
Re: [ccp4bb] Distinguish NH4 from Na?
Dear Jacob, You may find useful this web site by Marjorie Harding about metal coordination sites in proteins http://tanna.bch.ed.ac.uk/ best regards Stefano Benini, Ph.D. Assistant Professor http://pro.unibz.it/staff2/sbenini/ Bio-organic Chemistry Laboratory Faculty of Science and Technology Free University of Bolzano Piazza Università, 5 39100 Bolzano, Italy Office (room K2.14): +39 0471 017128 Laboratory (room E.012): +39 0471 017901 Fax: +39 0471 017009 *** -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jacob Keller Sent: Wednesday, 16 November, 2011 19:21 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Distinguish NH4 from Na? Dear Crystallographers, I have crystals containing 666mM NH4 and 540mM Na, and there appears to be a water which is only about 2.2 Ang from some polar atoms. It is currently reasonably happy as a Na, but is there any reasonable way to decide which cation is there? JPK -- *** Jacob Pearson Keller Northwestern University Medical Scientist Training Program email: j-kell...@northwestern.edu ***
[ccp4bb] PhD position in structural biology at the Free University of Bolzano
Dear All, On behalf of the Faculty of Science and Technology (http://www.unibz.it/en/sciencetechnology/welcome/default.html) of the Free University of Bolzano (http://www.unibz.it/en/public/university/default.html) I am glad to advertise a position for a PhD on the structural biology of proteins from plants' bacterial pathogens within our multidisciplinary PhD program in Management of Mountain Environment. Information on the PhD program as well as the forms required to apply (applications should be received by October 14th ) can be found at the following links: http://www.unibz.it/en/sciencetechnology/progs/phd/phdmountainenvironment/default.html http://www.unibz.it/en/public/research/phd/prospectivePhdstudents.html With my best regards Stefano Benini Stefano Benini, Ph.D. Assistant Professor http://pro.unibz.it/staff2/sbenini/ Bio-organic Chemistry Laboratory Faculty of Science and Technology Free University of Bolzano Piazza Università, 5 39100 Bolzano, Italy Office (room K2.14): +39 0471 017128 Laboratory (room E.012): +39 0471 017901 Fax: +39 0471 017009 ***
[ccp4bb] Enterokinase cleavage of His-tagged proteins
Dear All, I decided to use a LIC vector with enterokinase cleavage site for His tag removal. Of course I did not check the length of the linker (about 45 aa) nor if the enterokinase cleavage site would be the best choice Do you know where I could purchase a decent quality with reasonable price enterokinase to cleave mgs of his-tagged proteins for crystallization? Thank you very much and best regards Stefano Stefano Benini, Ph.D. Assistant Professor http://pro.unibz.it/staff2/sbenini/ http://pro.unibz.it/staff2/sbenini/ Bio-organic Chemistry Laboratory Faculty of Science and Technology Free University of Bolzano Piazza Università, 5 39100 Bolzano, Italy Office (room K2.14): +39 0471 017128 Laboratory (room E.012): +39 0471 017901 Fax: +39 0471 017009 ***
[ccp4bb] which Linux workstation for crystallography to buy?
Dear All, I need to buy a Linux workstation to run crystallographic software and graphics like ccp4, mosflm, coot., etc., Could you please suggest me a good combination of hardware and which linux operating system to install (ubuntu?)? I can spend about 1500€ Technology evolves so fast that I really want to be up to date not to be already late! Thank you very much in advance Stefano Stefano Benini, Ph.D. Assistant Professor http://pro.unibz.it/staff2/sbenini/ Bio-organic Chemistry Laboratory Faculty of Science and Technology Free University of Bolzano Piazza Università, 5 39100 Bolzano, Italy Office (room K2.11): +39 0471 017128 Laboratory (room E.012): +39 0471 017901 Fax: +39 0471 017009 ***
[ccp4bb] Off topic: multidisciplinary international PhD program in Bolzano, Italy
Dear All, on behalf of the Faculty of Science and Technology of the Free University of Bolzano, I would like to announce our new multidisciplinary international PhD program The PhD official language is English. The research projects cover several scientific fields: ecology, hydrology, fruit science, agricultural chemistry, biology, structural biology, microbiology, etc., More information are available at the following links: PhD Programme in Management of Mountain Environment: http://www.unibz.it/en/sciencetechnology/progs/phdmountainenvironment/default.html http://www.unibz.it/en/sciencetechnology/progs/phdmountainenvironment/default.html topics of PhD Thesis: http://www.unibz.it/en/sciencetechnology/progs/phdmountainenvironment/thesis/default.html http://www.unibz.it/en/sciencetechnology/progs/phdmountainenvironment/thesis/default.html study program: http://www.unibz.it/en/sciencetechnology/progs/phdmountainenvironment/studyplan/default.html http://www.unibz.it/en/sciencetechnology/progs/phdmountainenvironment/studyplan/default.html application forms: http://www.unibz.it/en/public/research/phd/prospectivePhdstudents.html http://www.unibz.it/en/public/research/phd/prospectivePhdstudents.html The Free University of Bolzano: http://www.unibz.it/en/public/university/default.html Bolzano is located in the north of Italy not far from the best slopes for alpine ski, hiking and climbing and the Dolomites (UNESCO heritage site http://whc.unesco.org/en/list/1237/). please do not reply to this email but if you are interested please refer to the links provided or contact: science.technol...@unibz.it mailto:science.technol...@unibz.it Faculty of Science and Technology piazza Università, 5 39100 Bolzano Italy T: +39 0471 017000 F: +39 0471 017009 With my best regards Stefano Benini, Ph.D. Assistant Professor http://pro.unibz.it/staff2/sbenini/ http://pro.unibz.it/staff2/sbenini/ Bio-organic Chemistry Laboratory Faculty of Science and Technology Free University of Bolzano-Bozen Piazza Università, 1 39100 Bolzano, Italy ***
