Agree with Tim's idea. Generating a composite omit map to see if your
solution is correct (or at least some part is correct if conformational
difference exists between model and your protein).
Bingfa
-邮件原件-
发件人: owner-ccp...@jiscmail.ac.uk [mailto:owner-ccp...@jiscmail.ac.uk] 代
表 Tim Gruene
发送时间: 2011年4月27日 17:10
收件人: CCP4BB@JISCMAIL.AC.UK
主题: Re: [ccp4bb] A phase ambiguity problem.
Dear Jashin,
do you see any extra density in the map, of unexplained features?
An easy and quick test is to remove some part of the search model, e.g. an
alpha-helix, and recalculate the map. If you do see density for the missing
helix, it is a very promising sign that your solution is correct.
If you don't see anything chances are that it is a false solution, but at
very low resolution, I would probably make further tests to be sure.
Tim
On Wed, Apr 27, 2011 at 04:28:49PM +0800, Jai-Shin Liu wrote:
Dear all:
A phase ambiguity problem.
After rotation/translation/refinement, the free value junk 0.5.
It seems no significant solution from rf file. INTERESTING, the density
map seems to fit the template very well.
In this case, is the phase still ambiguity or not?
Thank you very much. Any comments will be appreciated.
Jashin
The rf file of molrep is below:
theta phi chi alpha beta gamma Rf Rf/sigma Sol_RF 1
156.17 161.35 93.59 27.11 34.26 244.41 648.4 3.81 Sol_RF 2 163.22
179.18 121.99 29.25 29.26 210.90 561.2 3.30 Sol_RF 3 140.62 -179.55
61.93 65.56 38.11 244.67 556.5 3.27 Sol_RF 4 162.13 178.18 52.79 62.89
15.68 246.54 548.7 3.23 Sol_RF 5 136.93 -168.34 80.84 69.77 52.57
226.45 533.9 3.14 Sol_RF 6 165.86 -154.33 119.55 56.67 24.37 185.33
525.6 3.09 Sol_RF 7 148.21 162.97 129.01 12.27 56.79 226.33 522.2 3.07
Sol_RF 8 165.70 -159.77 120.00 51.02 24.71 190.56 512.9 3.02 Sol_RF 9
164.58 -171.65 121.34 38.58 26.80 201.89 511.6 3.01 Sol_RF 10 144.59
159.55 61.67 43.61 34.56 264.50 503.4 2.96
--
--
Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
phone: +49 (0)551 39 22149
GPG Key ID = A46BEE1A
