[ccp4bb] Postdoctoral research opportunity
Hello everyone, A postdoc position is available in my lab to study the structural biology of infectious and genetic diseases. Projects with crystals in-hand are available in two areas: 1) Inhibitor design projects targeting coronavirus proteases and proteases of related viruses, 2) Structure/function studies of lysosomal enzymes involved in genetic disease and the development of pharmacological chaperones to rescue their function. For those with Cryo-EM experience, an additional project focused on understanding the structural biology of large viral polymerases is also available. Our lab has access to in-house X-ray diffraction instrumentation (Rigaku), on-going access to synchrotron sources including the Canadian Light Source, and a new in-house Talos F200C Cryo-EM outfitted with a Falcon 4 direct electron detector. Additional information about our research and recent publications can be found at: http://home.cc.umanitoba.ca/~bmark/Welcome.html Enthusiastic individuals who hold a PhD in biochemistry or related field are encouraged to apply. Candidates should have a strong background in molecular biology and protein X-ray crystallography. Experience in Cryo-EM would be an asset given projects available. We are in the Department of Microbiology, University of Manitoba, Winnipeg, Canada. The University of Manitoba (http://umanitoba.ca/) hosts over 30,000 students, faculty, and staff and has a dynamic biomedical research community. Winnipeg is a vibrant, multicultural city with numerous cultural events and festivals (https://www.tourismwinnipeg.com). Salary will be in accordance with Canadian Institutes of Health Research (CIHR) (http://www.cihr.ca/ ) standards and includes a university staff benefits package. All qualified candidates are encouraged to apply. Please email inquiries and CV's (including names of three referees) to Brian Mark (brian.m...@umanitoba.ca). All the best, Brian Mark == Brian Mark, MSc, PhD Dean, Faculty of Science Professor, Department of Microbiology & Department of Biochemistry and Medical Genetics University of Manitoba 247 Machray Hall 186 Dysart Road Winnipeg, Manitoba R3T 2N2 PH: 204-474-9348 Research Lab: http://home.cc.umanitoba.ca/~bmark/ Biochem lab: (204) 480-1430 X-ray lab: (204) 474-8334 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] Job opportunity
Hello everyone, I’d like to draw your attention to a job posting in the Faculty of Science, University of Manitoba, Canada. We seek a technician to maintain and provide training on our X-ray diffraction and microscopy equipment in the faculty of science. Please use the URL below to access the full job posting. https://umanitoba.hua.hrsmart.com/ats/js_job_details.php?reqid=20906 Cheers, Brian Mark == Brian Mark, MSc, PhD Associate Professor Department of Microbiology & Department of Biochemistry and Medical Genetics Mailing/Courier address: Department of Microbiology Rm 213 Buller Building University of Manitoba 45 Chancellor's Circle Winnipeg, Manitoba CANADA R3T 2N2 Phone (204) 480-1430 Fax (204) 474-7603 Web: http://home.cc.umanitoba.ca/~bmark/Welcome.html
[ccp4bb] Assistant Professor position available
Dear CCP4 community, An Assistant Professor position in Microbiology or Biochemistry (Position # 22809) is currently open in the Department of Microbiology, University of Manitoba, Canada. Structural biology (mainly X-ray crystallography) is a key strength of our Department and so we are encourage applicants with such a background to apply. Please see the ad below for details. For further information contact the Search Committee Chair at mailto:deborah.co...@umanitoba.ca>>. The closing date for receipt of applications is Friday, January 6, 2017 ——— The Department of Microbiology invites applications for a full-time tenure-track position at the Assistant Professor level, commencing July 1, 2017, or on a date hing and research. Outstanding candidates in any area of Microbiology or Biochemistry will be considered, with particular emphasis on candidates who will complement or extend the department’s strengths. The successful candidate will have a Ph.D. and preferably post-doctoral experience or industry experience or other distinguishing attributes in host-microbe interactions, antibiotic resistance and drug discovery, structural biology, protein and membrane biochemistry, systems microbiology, and industrial and applied microbiology, or a related field. Duties will involve research, including the establishment of an externally funded research program, undergraduate teaching, graduate teaching and supervision, and service-related activities. The successful candidate will have a track record of high quality scholarly research leading to peer assessed publications; will either have, or demonstrate the potential to establish, an independent, innovative, scholarly, externally fundable research program; will have demonstrated strength in or strong potential for outstanding teaching contributions; and will exhibit evidence of the ability to work in a collaborative environment. Salary will be commensurate with experience and qualifications. To enhance our department and create role models for a diverse population of students, we particularly invite application from those who can support and enhance our diversity, including women, Indigenous peoples, other visible minorities, and those committed to a diverse environment. The Department currently has twelve full time tenured and tenure track faculty members and three Instructors, and offers a full range of both undergraduate and graduate programs in Microbiology and Biochemistry. The Department has a well-established and equipped research facility, including X-ray crystallography and large scale mammalian and prokaryotic cell culture facilities, as well as access to state of the art light microscopy, electron microscopy, and small angle x‑ray scattering instrumentation, and is supported by strong research links with other University of Manitoba departments including Chemistry, Biological Sciences, Physics, Biosystems Engineering, Biochemistry and Medical Genetics, and Plant Science and with the National Microbiology Laboratory of the Public Health Agency of Canada in Winnipeg. Further information about the Department can be obtained from http://sci.umanitoba.ca/microbiology>>. Winnipeg is the largest city in the Province of Manitoba. The city has a rich cultural environment, including symphony, opera, dance, theatre, and ethnic festivals and professional hockey and football. The region provides ample opportunities for outdoor recreation in all seasons. Learn more about Winnipeg at . The University of Manitoba is strongly committed to equity and diversity within its community and especially welcomes applications from women, members of racialized communities, Indigenous persons, persons with disabilities, persons of all sexual orientations and genders, and others who may contribute to the further diversification of ideas. All qualified candidates are encouraged to apply; however, Canadian citizens and permanent residents will be given priority. Applications including a curriculum vitae, a description of teaching philosophy, a summary of research interests, a three page research plan and contact information for three references should be sent to (a single PDF file is preferred). Please ensure to specify position number 22809 in the application. For further information contact the Search Committee Chair at . The closing date for receipt of applications is Friday, January 6, 2017. Application materials, including letters of reference, will be handled in accordance with the Freedom of Information and Protection of Privacy Act. Please note that curricula vitae may be provided to participating members of the search process. Brian ====== Brian Mark, MSc, PhD Associate Professor Department of Microbiology & Department of Biochemistry and Medical Genetics Mailing/Courier address: Department of Microbiology Rm 213 Buller Building Universit
Re: [ccp4bb] anti-His6 Ab
Hi Elias, Not sure if you've looked at this option, but Pierce sells a polyHis tag detection kit that is not based on an antibody, but instead is a "nickel-activated" derivative of HRP. Has worked quite well for us in the past. Brian Mark On 2013-03-15, at 12:36 PM, Elias Fernandez mailto:efern...@utk.edu>> wrote: Dear All, would you be able to recommend a primary anti-His6 Ab for western blotting? Thus far, the ones we’ve used are not very specific to the N-terminal hexahistidines and we pick up lots of background. Also, there’s one (others?) from Sigma that is conjugated to HRP, but costs ~$650. Thanks! Elias
[ccp4bb] Postdoctoral research opportunity
Greetings everyone. I'd like to call your attention to the following postdoc opportunity: Postdoctoral research opportunity in antibiotic drug resistance Applications are invited for a postdoctoral position to study the structural biology of proteins and enzymes that comprise AmpC beta-lactamase induction pathway. Beta-lactam antibiotics act to disrupt bacterial cell wall metabolism. Numerous bacterial pathogens respond to this disruption by inducing the expression of AmpC beta-lactamase. AmpC is of considerable clinical concern since it confers broad-spectrum resistance against beta-lactams. We focus on understanding the structural biology of bacterial cell wall metabolism and the development small molecule based strategies to block AmpC gene expression during antibiotic therapy. Additional information about our research and recent publications in the area can be found at: http://home.cc.umanitoba.ca/~bmark/Welcome.html We seek an enthusiastic, hard working individual who holds a PhD in biochemistry or related field. Candidates should have a strong background in molecular biology and protein X-ray crystallography. Experience in membrane protein crystallography would be an asset. We are located in the Department of Microbiology, University of Manitoba, Winnipeg, Canada. The laboratory offers state-of-the-art X-ray instrumentation (Rigaku), crystallization robotics (Art Robbins), and on-going user access to the Canadian Light Source Synchrotron via mail-in service, remote robotics, or in-person visits. The University of Manitoba (http://umanitoba.ca/) is the largest University in the province (over 30,000 students, faculty, and staff) and hosts a dynamic biomedical research community. Winnipeg is a vibrant, multicultural city with numerous cultural events and festivals (http://www.destinationwinnipeg.ca/). Salary will be in accordance with the Canadian Institutes of Health Research (CIHR) (http://www.cihr.ca/ ) standards. The position also includes a University benefits package. All qualified candidates are encouraged to apply. Please direct inquiries, including a cover letter, brief description of your career goals and CV (including names of three referees) to Brian Mark at the following email address: brian.m...@ad.umanitoba.ca<mailto:brian.m...@ad.umanitoba.ca> ===== Brian Mark, MSc, PhD Associate Professor Manitoba Research Chair in Structural Biology Department of Microbiology & Department of Biochemistry and Medical Genetics Mailing/Courier address: Department of Microbiology Rm 418 Buller Building University of Manitoba 45 Chancellor's Circle Winnipeg, Manitoba CANADA R3T 2N2 Phone (204) 480-1430 Fax (204) 474-7603 Web: http://home.cc.umanitoba.ca/~bmark/Welcome.html
[ccp4bb] Postdoctoral position available
POSTDOCTORAL POSITION AVAILABLE Applications are invited for a postdoctoral position to investigate the structural and cellular biology of viral immune evasion tactics and assembly mechanisms of Bunyaviruses, the largest family of negative-strand RNA viruses. The family Bunyaviridae includes a number of emerging viruses of human and agricultural importance and much remains to be discovered about their life cycle and the mechanisms they use to subvert the innate immune system. The following is an example of our recent work in this area: James TW, et al. (2011). Proc Natl Acad Sci U S A. 108(6):-7. A number of exciting projects are underway and ready to carry forward. More information about our lab can be found at: http://home.cc.umanitoba.ca/~bmark/Welcome.html We seek enthusiastic applicants who hold a PhD in biochemistry or related field. Candidates must have a strong background in molecular biology and protein X-ray crystallography. Expertise in mammalian cell culture and fluorescence microscopy would be considered strong assets. Our group is located in the Department of Microbiology, University of Manitoba, Winnipeg, Canada. The laboratory includes state-of-the-art X-ray instrumentation (Rigaku) and crystallization robotics (Art Robbins). We have routine access to the Canadian Light Source Synchrotron via mail-in service, remote control of robotics, or in person (within driving distance). The University of Manitoba (http://umanitoba.ca/) is the largest University in the province (over 30,000 students, faculty, and staff) and hosts a dynamic biomedical research community. Winnipeg is a vibrant, multicultural city with numerous cultural events, festivals and a brand new NHL hockey team (http://www.destinationwinnipeg.ca/). With over 110,000 fresh water lakes in our province alone, there is also much to explore for those who love the outdoors. Salary will be in accordance with the Canadian Institutes of Health Research (CIHR) (http://www.cihr.ca/ ) standards. Fellowship funding is also available through agencies such as the CIHR and the Manitoba Health Research Council (MHRC) (http://www.mhrc.mb.ca/). All qualified candidates are encouraged to apply. Please direct formal and informal inquiries and CV's (including names of three referees) to my email address: brian_m...@umanitoba.ca = Brian Mark, MSc, PhD Associate Professor Manitoba Research Chair in Structural Biology Department of Microbiology & Department of Biochemistry and Medical Genetics Mailing/Courier address: Department of Microbiology 418 Buller Building University of Manitoba Winnipeg, Manitoba CANADA R3T 2N2 Phone (204) 480-1430 Fax (204) 474-7603 Web: http://home.cc.umanitoba.ca/~bmark/Welcome.html
[ccp4bb] 96-well block storage
Hi all, Considering the popularity of 96-well deep well block format for purchasing and storing protein crystallization conditions, I'm surprised that a better re-sealing system has not been invented to prevent evaporation from the blocks when they are stored. We typically don't consume our blocks fast enough to avoid this issue. There must be a better way to re-seal these blocks other than using peel and stick foil tape over and over again... Would anyone like to share their optimal method for storing 96-wells blocks that avoids (or a least minimizes) evaporation? Cheers, Brian Mark
[ccp4bb] postdoctoral position
Postdoctoral position available: Applications are invited for a postdoctoral position to investigate the structure and biochemistry of proteins involved in Gram-negative bacterial cell wall recycling. Peptidoglycan metabolites produced during cell wall turnover are found to regulate an increasing number of bacterial processes, including the induction of antibiotic resistance in a number bacterial species that cause opportunistic infections in humans. We are focused on understanding the biology of the cell wall recycling pathway, with the goal of developing small molecule based strategies to disrupt the pathway and suppress antibiotic resistance mechanisms that are coupled to it (J. Biol. Chem. 2007; 282:21382-91, 2009 Antimicrob Agents Chemother. 2009 53:2274-82, Protein Sci. 2009;18:1541-51). A number of projects are well advanced, some with crystals already in-hand. More project information can be found at: http://umanitoba.ca/faculties/science/microbiology/staff/mark/ We seek enthusiastic applicants who hold a PhD in biochemistry or related field. Candidates should have a strong background in molecular biology, protein purification/crystallization and protein X-ray crystallography. Our group is located in the Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada. The laboratory includes state-of- the-art Rigaku instrumentation (Micromax 007 HF, X-stream cryosystem and R-axis 4++ detector) and has access to the newly commissioned Canadian Light Source Synchrotron, which is a short drive away. The University of Manitoba (http://umanitoba.ca/) is the largest University in the province (over 30,000 students, faculty, and staff) and hosts a large, interactive biomedical research community. Winnipeg is a vibrant, multicultural city with an outstanding performing arts scene, numerous cultural events and festivals, and great dining (http://www.destinationwinnipeg.ca/). With over 110,000 fresh water lakes in our province alone, there is also much to explore for those who love the outdoors. Salary will be in accordance with the Canadian Institutes of Health Research (CIHR) (http://www.cihr.ca/ ) standards. Fellowship funding is also available through agencies such as the CIHR and the Manitoba Health Research Council (MHRC) (http://www.mhrc.mb.ca/). The University of Manitoba encourages applications from qualified women and men, including members of visible minorities, Aboriginal peoples, and persons with disabilities. All qualified candidates are encouraged to apply. Please direct inquiries and CV's (including names of three referees) to the following email address: brian_m...@umanitoba.ca = Brian L. Mark, MSc, PhD Assistant Professor Department of Microbiology Room 418, Buller Building University of Manitoba Winnipeg, Manitoba CANADA R3T 2N2 Phone (204) 480-1430 Fax (204) 474-7603 Web: http://www.umanitoba.ca/science/microbiology/staff/mark/
Re: [ccp4bb] Crystallographic computing platform recommendations?
Francis, From your response and others to my question about OS X server 10.5, AFP seems to be the preferred networking protocol over NFS. Yes, in our case the RAID is connected to a G5 (via firewire 800 - which provides surprisingly good transfer rates BTW) that is running OS X server 10.5 . I'll try AFP for the user home directories. Thanks, Brian On 18-Nov-08, at 6:21 PM, Francis E Reyes wrote: On Nov 18, 2008, at 9:57 AM, Brian Mark wrote: Hi all, With all the talk about Mac OS X, I've not heard much mention about OS X Server and networking Macs together. Is anyone using the 10.5 server and LDAP to centrally house user directories on a RAID connected to a Mac server for example? We run 10.5 server with OpenDirectory (LDAP for Apple) over AFP (no raid) with 10.5 clients. We had this setup running with 10.4, but we now seem to have issues getting the server 10.5 to export the RAID to the clients via NFS. I know NSF is old, but our network is behind a firewall and it has worked well for us. It seem if you don't set things up the way Apple recommends (using AFP for example), things can get difficult... It's quite possible that we are missing something during the setup, but frankly I'm surprised at how difficult it has been to work with OS X server. Is the RAID directly connected to the server? i.e. does OS X server host the nfs server or is it a unix machine? the requirements for specifying an nfs home dir are tricky. Check the Admin reference volume 1 book. I would be happy to hear about other people's experience with OS X server 10.5. Perhaps a website describing OS X server for crystallographic computing/networking would be nice if it doesn't exist already. As I said above we have leopard clients bound to an OSX server using OD and AFP home directories. All the clients are imaged to the same version of OSX Leopard and crystallography software (managed by fink mostly). If there's a prudent need for upgrading, I have a bootable firewire drive that I load onto one of the clients and do the updating, then image the software to the server and use Netboot/ Netinstall to distribute the image to the clients. Any more questions let me know FR - Francis Reyes M.Sc. 215 UCB University of Colorado at Boulder gpg --keyserver pgp.mit.edu --recv-keys 67BA8D5D 8AE2 F2F4 90F7 9640 28BC 686F 78FD 6669 67BA 8D5D
Re: [ccp4bb] Crystallographic computing platform recommendations?
Hi all, With all the talk about Mac OS X, I've not heard much mention about OS X Server and networking Macs together. Is anyone using the 10.5 server and LDAP to centrally house user directories on a RAID connected to a Mac server for example? We had this setup running with 10.4, but we now seem to have issues getting the server 10.5 to export the RAID to the clients via NFS. I know NSF is old, but our network is behind a firewall and it has worked well for us. It seem if you don't set things up the way Apple recommends (using AFP for example), things can get difficult... It's quite possible that we are missing something during the setup, but frankly I'm surprised at how difficult it has been to work with OS X server. I would be happy to hear about other people's experience with OS X server 10.5. Perhaps a website describing OS X server for crystallographic computing/networking would be nice if it doesn't exist already. Cheers, Brian = Brian L. Mark, MSc, PhD Assistant Professor Department of Microbiology Room 418, Buller Building University of Manitoba Winnipeg, Manitoba CANADA R3T 2N2 Phone (204) 480-1430 Fax (204) 474-7603 Web: http://www.umanitoba.ca/science/microbiology/staff/mark/ On 18-Nov-08, at 9:42 AM, Anastassis Perrakis wrote: On Nov 18, 2008, at 16:26, William G. Scott wrote: On Nov 18, 2008, at 7:01 AM, Mischa Machius wrote: For linux, I would recommend a commercial solution, For linux, I actually ditched the commercial solution for Ubuntu, because it was vastly easier as a non-expert to maintain. Having said that, like you, I have found running Mac OS X to be the most cost-effective in terms of time and utility. Same here. Lets also say that I have seen many people in the lab switch from Linux and Windows to OSX, but nobody from OSX back to Windows or Linux. That must be saying something. We still use btw Linux workstations for stereo and I am happy with them as well. As Harry says, choose what you are most comfortable with(*). A. (*) If thats Windows Vista though, take a break and meditate, think of the bigger picture of life, donate to the Bill Gates foundation which is what Bill Gates should be remembered for, and find the nearest MacStore. ;-) Bill
Re: [ccp4bb] Co-expression plasmids
Hi Mark, Proper co-expression in trans requires that you use plasmids each with different origins of replication and antibiotic markers, such as p15 + Kan and ColE1 + Amp. I think the pET vectors of the DUET system use these two origins (p15 and ColE1). This ensures much more efficient dual transformation and proper plasmid stability over time. The two backbone constructions above are used in a variety of pET vectors, so if you look for this detail, you can co-transform and co-express from many available plasmids and customize as you like. Suppliers will tell you the origins that are used in each of the plasmids they sell. Cheers, Brian = Brian L. Mark, MSc, PhD Assistant Professor Department of Microbiology Room 418, Buller Building University of Manitoba Winnipeg, Manitoba CANADA R3T 2N2 Phone (204) 480-1430 Fax (204) 474-7603 Web: http://www.umanitoba.ca/science/microbiology/staff/mark/ On 2-Apr-08, at 8:55 AM, Mark J. van Raaij wrote: Dear All, Anyone have experience with the NovaGen Duet co-expression vectors? Or can recommend others? http://www.emdbiosciences.com/html/NVG/Duet_Spot.html Greetings, Mark Mark J. van Raaij Dpto de Bioquímica, Facultad de Farmacia Universidad de Santiago 15782 Santiago de Compostela Spain http://web.usc.es/~vanraaij/
[ccp4bb] Additive screens
Hello everyone, Does anyone have suggestions or comments on the various additive screens that are available? I was looking at the kit from Hampton (HR2-138 Additive Screen HT) in 96-well format. It appears that it should be straight forward to array out our current protein/mother liquor condition into a 96-plate and then add in the additives from the deep-well block provided by the company. The additives come 10x concentrated in a 96-well deep- well block for dilution into the arrayed crystallization condition. Does anyone do this routinely for the crystals they grow? It would be interesting to know how often people find a systematic approach using such a kit actually improves the quality/size etc. of their crystals. Cheers, Brian --- Brian L. Mark, MSc, PhD Department of Microbiology Room 418, Buller Building University of Manitoba Winnipeg, Manitoba CANADA R3T 2N2 Phone (204) 480-1430 Fax (204) 474-7603 Web: http://www.umanitoba.ca/science/microbiology/staff/mark/
