[ccp4bb] Structural Biology Position in Newcastle Cancer Centre Drug Discovery Unit
Dear all, Could I draw your attention to a great position that wehave available to join a two year, MRC DPFS-funded project to providestructural and biophysical input to a drug discovery project in the CRUKNewcastle Drug Discovery Unit. The project is pursuing an exciting structural hypothesisto develop therapies to address an unmet need for treatments for patients withovarian cancer. It is currently in early lead optimisation, and the post holderwill be joining a well-founded team to develop and apply techniques ofstructural biology (crystallography/cryo-EM), biophysics (SPR/ITC), andcellular and cell-free assays to progress the project towards clinicalcandidate selection. Because the work will be conducted in a group that hasbeen instrumental in the development of licensed drugs, this post will offerthe holder an insight into authentic and effective modern structure based drugdiscovery. It would especially suitsomeone looking to round off their structural biology skills with a view topursuing careers in academic or industrial drug discovery. Informal approaches can be made directly to Martin Noble(martin.no...@newcastle.ac.uk)or Jane Endicott (jane.endic...@newcastle.ac.uk),but anyone considering the position should apply through the Newcastle Universityportal using the URL: https://jobs.ncl.ac.uk/job/Newcastle-Research-AssistantAssociate/839154501/ This link also offers further particulars for the post,for which the closing date is 8th September 2022. Best wishes, Mat Dr Mathew Martin Cancer Research UK Newcastle Drug Discovery UnitTranslational and Clinical Research Institute Discovery of Medicines PaulO'Gorman Building Medical SchoolNewcastle University Framlington Place Newcastle upon TyneNE2 4HHEmail: mathew.mar...@ncl.ac.ukCRUK Newcastle DDU - Cancer Research UK Newcastle Drug Discovery Unit | Cancer Research UK Newcastle Drug Discovery Unit | Newcastle University | | | | Cancer Research UK Newcastle Drug Discovery Unit | Cancer Research UK Ne... | | | To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] PhD opportunity - Drug Discovery Newcastle University
Dear All, May I draw your attention to an opportunitythat we have for a talented student to join our drug-discovery team here at theNorthern Institute for Cancer Research. The position is a fully-funded PhDstudentship from the EPSRC Centre for Doctoral Training in Molecular Sciencesfor Medicine (MoSMed) supervised by Prof. Martin Noble. (MOS19-03) Protein aptamers to enable structure-baseddrug discovery – making the undruggable druggable. https://bit.ly/2Fh5bHT The deadline is today - 15thMarch 2019. To apply click on link PhD Studentships | Molecular Sciences for Medicine | Newcastle University | | | | PhD Studentships | Molecular Sciences for Medicine | Newcastle University | | | Best Wishes, Mat To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
Re: [ccp4bb] Codon Optimization for Insect Expression
Hi Randy and Rodger, We have had real success using BlueSky (MA) for insect expression, I believe we have had around 15-20 genes synthesis-optimized with these guys. The expression levels have been rather reasonable too (obviously protein dependent, but on average 1 mg of xtal grad protein per 1g of cell pellet). The largest construct we did I believe was around 90kDa. Cheers, Mat From: Roger Pickman To: CCP4BB@JISCMAIL.AC.UK Sent: Thursday, 9 May 2013, 10:31 Subject: Re: [ccp4bb] Codon Optimization for Insect Expression I hope this isn't too much of a thread hijack, but i also wanted to add a question to Randy's : How big a protein can one express in insect cells? I've read in a few places that it's not size limited but i wonder if that's borne out in practice. On 9 May 2013 16:05, Randy Watson wrote: Hi all, >Sorry for the off-topic request. I am considering ordering codon-optimized genes for expression in insect cells (High Five). I wonder if anyone could make suggestions regarding: which companies are best to order from (accurate synthesis – cost efficiency) and what optimized codon sequence generators yield the best protein expression (as I understand it, not all algorithms are created equal). >Thanks, >Randy
Re: [ccp4bb] Program or server to predict Kd from complex structure
Hi Wei, I'm unsure if this will help, as i've never used it myself, but a former colleague in France is working on such a server? http://2p2idb.cnrs-mrs.fr/ But as everyone else has stated, only use this in comparison to some hard physical data. Cheers, Mat From: Ed. Pozharski To: CCP4BB@JISCMAIL.AC.UK Sent: Thursday, 18 April 2013, 8:46 Subject: Re: [ccp4bb] Program or server to predict Kd from complex structure Don't believe such program/server does exist. Notice that you are asking for something that *can* predict Kd. One can *try* making such predictions and they may even be routinely in the ballpark, assuming that you are satisfied with being routinely off by, say, an order of magnitude. One can easily predict general trends. For example, larger buried apolar surface will generally result in lower Kd. As for individual Kd prediction accuracy, that's another story. It's unknown to me what your goal is, but if you are trying to replace experimental Kd determination with a magic program, please don't. Cheers, Ed. Original message From: Wei Liu Date: 04/18/2013 4:39 AM (GMT-05:00) To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Program or server to predict Kd from complex structure Dear all, Does anyone know a program or web server that can predict Kd value between two proteins from a solved complex structure? Regards Wei
Re: [ccp4bb] Structure example request for large domain movement "in crystallo" soaking
Dear Wenhe, We too have had similar experiences with a couple of other other kinases - CDK2 (PMID: 21291269) and Aurora A (PMID: 22248356), where both soaking and co-crystallization has resulted in large conformational rearrangement. Best wishes, Mat. From: WENHE ZHONG To: CCP4BB@JISCMAIL.AC.UK Sent: Tuesday, 9 October 2012, 9:33 Subject: [ccp4bb] Structure example request for large domain movement "in crystallo" soaking Dear CCP4 members, Recently, I got a ligand soaking structure to clearly show a large domain (~100 amino acids) movement compared to the no soaking structure. Although there are some reported examples of this enzyme to suggest the flexibility of this large domain which is relevant to substrate binding. But it is the first time I can see it happen in crystal soaking procedure. In this case, I am pleased by this result. My question is, do you have any other example like mine, where domain (or loop) movement is observed in crystal during ligand soaking procedure? It would be very helpful for me to relate my result to other similar examples. Thank you very much. King regards, Wenhe
