Re: [ccp4bb] A postdoctoral position in structural biology is available at UT Southwestern Medical Center at Dallas
CryoEM post-doctoral positions are available at the Duke-NUSGraduate Medical School, Singapore. Duke-NUS Graduate Medical School is a global partnership betweenDuke University in the US and the National University of Singapore. Two post-doctoralpositions with competitive salary packages are available at the laboratory ofShee-Mei Lok (http://www.loksheemeilab.com/) in the Emerging Infectious Diseaseprogram of the Duke-NUS. Projectsundertaken by the post-doc will lead to further understanding of the neutralizationmechanism of various antiviral therapeutics against flavivirus/alphaviruses viruses.Individuals with experience in x-ray crystallography and/or cryoEM imagereconstruction are highly desirable. Our lab uses the cryoEM facility atthe National University of Singapore (NUS), which houses a Titan Krios. We alsocollaborate extensively with other biophysical groups in Singapore that usetechniques such as Deuterium Hydrogen Exchange Mass Spectrometry (DHXMS) andMolecular Dynamic Simulations (MDS). Successful applicants will haveopportunities to learn across multiple disciplines for studying virus structures:virology, immunology, x-ray crystallography, cryoEM single particle analysis,CryoEM tomography, DHXMS and MDS. Singapore is a modern city state andwith easy access to nearby exotic destinations in South East Asia. We also haveexcellent Asian cuisines. Anthony Bourdain on Singapore’s food scene: “Singapore is possibly the most food-centric place on Earth, with themost enthusiastic diners, the most varied and abundant, affordable dishes —available for cheap — on a per-square-mile basis. The hawker centers(basically, food courts where individually-owned mom and pop operations servestreet food from tiny shops and booths) are wonderlands of Chinese, Indian, andMalay specialties. It’s like shooting fish in a barrel making food porn inSingapore.” Interestedindividuals should send their CV, experience, research interest, and a list ofthree referees to Dr Shee-mei Lok at this email: sheemei@duke-nus.edu.sg. To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
[ccp4bb] Postdoctoral positions available in Duke-NUS, Singapore
CryoEM post-doctoral positions are available at the Duke-NUS Graduate Medical School, Singapore. Duke-NUS Graduate Medical School is a global partnership between Duke University in the US and the National University of Singapore. Two post-doctoral positions with competitive salary packages are available at the laboratory of Shee-Mei Lok (http://www.loksheemeilab.com/) in the Emerging Infectious Disease program of the Duke-NUS. Projects undertaken by the post-doc will lead to further understanding of the neutralization mechanism of various antiviral therapeutics against flavivirus/alphaviruses viruses. Individuals with experience in x-ray crystallography and/or cryoEM image reconstruction are highly desirable.Interested individuals should send their CV, experience, research interest, and a list of three referees to Dr Shee-mei Lok at this email:sheemei@duke-nus.edu.sg. Regards, Shee-Mei LokAssociate Professor/National Research Foundation InvestigatorProgram in Emerging Infectious DiseasesDuke-NUS Medical SchoolKTP BuildingLevel 9 8 college RoadSingapore 169857Ph:65-65165840 Associate Professor/Principal InvestigatorCenter for BioImaging SciencesDepartment of Biological SciencesNational University of SingaporeSingapore To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
[ccp4bb] Two postdoctoral positions available at Duke-NUS Singapore working on cryoEM
Crystallography or cryoEM post-doctoralpositions are available at the Duke-NUS Graduate Medical School, Singapore. Duke-NUSGraduate Medical School is a global partnership between Duke University in theUS and the National University of Singapore. Two post-doctoral positions with competitive salary packages are availableat the laboratory of Shee-Mei Lok (http://www.loksheemeilab.com/) in theEmerging Infectious Disease program of the Duke-NUS. Projects undertaken by the post-doc will leadto further understanding of the neutralization mechanism of various antiviraltherapeutics against flavivirus viruses. Individuals with experience in x-ray crystallographyand/or cryoEM image reconstruction are highly desirable.Interested individuals should send their CV,experience, research interest, and a list of three referees to Dr Shee-mei Lok atthis email: sheemei@duke-nus.edu.sg.
[ccp4bb] postdoctoral position available in Singapore, Duke-NUS
Post-doctoral position in Singapore at the Duke-NUS is available A post-doctoral position is available at the laboratory of Shee-Mei Lok (http://www.loksheemeilab.com) in the emerging infectious disease program of the Duke-NUS. Duke-NUS graduate medical school situated in Singapore, is a global partnership between Duke University and the National University of Singapore. We have the state of the art facility for both x-ray crystallography and also cryoelectron microscopy (Titan Krios with Falcon II detector, Jeol microscope with phase plate, energy filter and DE camera). Projects undertaken by the post-doc will mainly investigate the structure and function of important proteins contributing to the longevity and low rates of cancer in bats. He/she will also take part in dengue virus research. In addition to crystallography, cryo-electron microscopy will also be used to determine the structures. Individuals with experience in electron microscopy and/or crystallography, protein expression and purification are highly desirable. Interested individuals should send their CV, experience, research interest, and a list of three referees to Shee-mei Lok (sheemei@duke-nus.edu.sg)
[ccp4bb] Post-doctoral position in Singapore at the Duke-NUS is available
Post-doctoral position in Singapore at the Duke-NUS is available A post-doctoral position is available at the laboratory of Shee-Mei Lok (http://www.loksheemeilab.com) in the emerging infectious disease program of the Duke-NUS. Duke-NUS graduate medical school situated in Singapore, is a global partnership between Duke University and the National University of Singapore. Projects undertaken by the post-doc will investigate the structure and function of important proteins contributing to the longevity and low rates of cancer in bats. Individuals with experience in crystallography, protein expression and purification are highly desirable. Interested individuals should send their CV, experience, research interest, and a list of three referees to Shee-mei Lok (sheemei@duke-nus.edu.sg)
[ccp4bb] PhD and Postdoctoral positions in structural virology at the Duke-NUS GMS
A PhD student and a postdoctoral position are available at The Duke-NUS GMS (Singapore) under the supervision of Dr Shee-Mei Lok. In our laboratory, we use a combination of techniques such as x-ray crystallography and cryo electron microscopy to investigate dengue virus complexes and its structural changeduring viral infection.We have the state-of-the-art facilities in cryoEM such as The FEI Titan Krios, phase plate, liquid cell system to investigate both frozen and liquid forms of the sample. For the postdoctoral position, candidates with a Ph.D in structural biology such as x-ray crystallography and cryoEM within 2-3 years of their graduation are encouraged to apply. Interested candidates are requested to send their CV, summary of research interests and names of 3 references to sheemei@duke-nus.edu.sg. Regards, Shee-Mei Lok Assistant Professor Program in Emerging Infectious Diseases, Duke-NUS Graduate Medical School, KTP Building Level 9 Room 19 8 college Road, Singapore 169857 Ph: 65-65165840 Fax:65-62212529
[ccp4bb] postdoc position in Singapore
A postdoctoral position is available at the Laboratory of Virus Structure and Function in the Duke-NUS Graduate Medical School, Singapore. The Laboratory of Shee-Mei Lok (http://www.duke-nus.edu.sg/web/research_faculty_sheemei.htm) is looking for a post-doctoral fellow to work on the structural studies of dengue viruses and its complexes. Her laboratory uses a combination of techniques such as x-ray crystallography and cryo-electron microscopy (CryoEM) including single particle analysis and cryotomography. Facilities such as in-house x-ray machines and cryoelectron microscope (Titan Krios, FEI) are available in the National University of Singapore. The post-doctoral fellow will mainly participate in the computational side of structure solving and should preferentially have prior experience in solving either cryoEM or x-ray structures. Training in crystallography or cryoEM data collection and image processing however, will also be available if necessary. Interested individuals are invited to send cover letter, cv and a list of referees to Dr Shee-Mei Lok by email (sheemei@duke-nus.edu.sg).
[ccp4bb] postdoctoral fellow position in Duke-NUS Graduate Medical School
A postdoctoral position is available at the Laboratory of Virus Structure and Function in the Duke-NUS Graduate Medical School, Singapore. The Laboratory of Shee-Mei Lok (http://www.duke-nus.edu.sg/web/research_faculty_sheemei.htm) is looking for a post-doctoral fellow to work on the structural studies of dengue viruses and its complexes. Her laboratory uses a combination of techniques such as x-ray crystallography and cryo-electron microscopy (CryoEM) including single particle analysis and cryotomography. Facilities such as in-house x-ray machines and cryoelectron microscope (Titan Krios, FEI) are available in the National University of Singapore. The post-doctoral fellow will mainly participate in the computational side of structure solving and thus should have prior experience in solving either cryoEM or x-ray structures. Training in crystallography or cryoEM data collection and image processing however, will also be available if necessary. Interested individuals are invited to send cover letter, cv and a list of referees to Dr Shee-Mei Lok by email (sheemei@duke-nus.edu.sg).
Re: [ccp4bb] Singapore Research, facts and comments?
Dear all, I work in Duke-NUS in Singapore. Overall the funding climate in Singapore is very good compared to most places in the world and the research quality has improved significantly to a world class standard in the past 10 years. Duke-NUS, NUS and NTU are Universities and we are used to writing grants to support all our research, like most Universities in the world. The complains by people working in A-star agency is because, they did not use to need to apply for any money. But now the government decides that it is time to wean people off the the easy money and compete for grant. Best, sheemei From: nadir.mra...@medecine.uhp-nancy.fr nadir.mra...@medecine.uhp-nancy.fr To: CCP4BB@JISCMAIL.AC.UK Sent: Sunday, 30 January 2011 07:58:18 Subject: Re: [ccp4bb] Singapore Research, facts and comments? Have a look at A* (http://www.a-star.edu.sg/). Best, Nadir -- Pr. Nadir T. Mrabet Structural Molecular Biochemistry INSERM U-954 UHP - Nancy 1, School of Medicine 54505 Vandoeuvre-les-Nancy Cedex France Tel : +33 (0)3.83.68.32.73 Fax : +33 (0)3.83.68.32.79 E-mail : nadir.mra...@medecine.uhp-nancy.fr Selon Jacob Wong jacob.j.w...@gmail.com: Thanks to those who has responded to me and asked for more specifics. I am interested in some most up-to-date (and published) facts/comments about the Biopolis, something that may follow up the article below that was published back in 2003. Singapore: filling biopolis. http://www.nature.com/nature/journal/v425/n6959/full/nj6959-746a.html Thank you, Jacob Wong On Sat, Jan 29, 2011 at 12:47 AM, Jacob Wong jacob.j.w...@gmail.com wrote: Dear All, sorry for this off-topic inquiry. I am interested in knowing about the current scientific research in Singapore but have had hard time finding the most up-to-date articles/comments on this. All I could find are the articles a few years back in Science and Nature. Would appreciate a pointer. Happy Chinese New Year! Thanks, Jacob Wong
Re: [ccp4bb] Native Gel Theory and Practice
How about using static light scattering to determine the actual molecular weight or dynamic light scattering to measure the diameter of the complex. Sheemei From: Jürgen Bosch jubo...@jhsph.edu To: CCP4BB@JISCMAIL.AC.UK Sent: Wednesday, 19 May 2010 11:00:24 Subject: Re: [ccp4bb] Native Gel Theory and Practice Here's just one example, which I quickly found from Reisinger and Eichacker. Isolation of membrane protein complexes by blue native electrophoresis. Methods Mol Biol (2008) vol. 424 pp. 423-31 Now Jacob has A 22 kDa B 17 kDa, the charge can be disregarded in BN PAGE. If we do the math for all the theoretical complexes and assume globular shape for all of them.78 AABB (22+22+17+17) 61 AAB (22+22+17) 56 ABB (22+17+17) 39 AB (22+17) 22 A 17 B I'd use a higher percentage gel 10-20% then you should be able to separate the 6 species mentioned above. Jürgen P.S. just trying to be helpful On May 19, 2010, at 11:40 AM, Maia Cherney wrote: Yes, you can separate by electrophoresis, that's why we use it, but we cannot calculate accurate mass of complexes. Maia Jürgen Bosch wrote: Not quite correct, look into Blue Native PAGE. There you can seperate natively by mass. Jürgen .. Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/ On May 19, 2010, at 1:31, Maia Cherney ch...@ualberta.ca wrote: Dear Jacob, I offer you my opinion. Are you talking about electrophoresis? As far as I know it does not work for the mass. The velocity of a protein depends on the charge at a particular pH, the mass and shape of molecules etc. It's very difficult to take all these things into consideration. Otherwise this would be a very convenient method, much easier than the analytical centrifugation or gel-filtration that are usually used. However, electrophoresis does not work for mass determination. Besides, complex formation hugely depends on the protein concentration. If you dilute your mixture, your complexes might dissociate. There is equilibrium constant between different types of complexes. Maia Jacob Keller wrote: Dear Crystallographers, I am trying to optimize a native gel experiment of a two-protein complex, running the smallest-detectable amount of protein component A with varying amounts of component B. MWCharge MW/Charge A 22 -5-4308 B 17-24 -702 This experiment is partly to determine stoichiometry, but also to determine roughly the strength of the interaction. B definitely runs much faster than A alone, as predicted, but I am wondering what to expect with various oligomers. Should ABB run faster or slower than AB? What about AABB? Theoretically, AA should certainly run slower than A, and BB slower than B, simply because the mass/charge ratio is the same, but the overall mass is greater. But what happens when you have AAB, for example? There must be an equation relating the mass/charge and mass (and perhaps gel percentage) to the speed traveled in the gel--but what is the equation? Thanks for your consideration, Jacob *** Jacob Pearson Keller Northwestern University Medical Scientist Training Program Dallos Laboratory F. Searle 1-240 2240 Campus Drive Evanston IL 60208 lab: 847.491.2438 cel: 773.608.9185 email: j-kell...@northwestern.edu *** - Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/
[ccp4bb] postdoctoral positions available in Duke-NUS, Singapore
Crystallography and/or cryoEM post-doctoral positions are available at the Duke-NUS Graduate Medical School, Singapore. Duke-NUS Graduate Medical School is a global partnership between Duke University in the US and the National University of Singapore. Post-doctoral positions with competitive salary packages are available at the laboratory of Shee-Mei Lok in the Emerging Infectious Disease program of the Duke-NUS. Projects undertaken by the post-doc will lead to further understanding of the neutralization mechanism of various antiviral therapeutics against flavivirus and influenza virus [Li et al., 2008 (Science 319:1830-4) and Lok et al., 2008 (Nat. Struct. Mol. Biol. 15:312-7)]. Individuals with experience in crystallography and/or cryoEM image reconstruction are highly desirable. Interested individuals should send their CV, experience, research interest, and a list of three referees to Dr Shee-mei Lok (sheemei@duke-nus.edu.sg).
