Re: [ccp4bb] refmac twin refinement vs resolution limit
Dear Eleanor, Thank you so much. As you said, it is a problem that the number of reflections estimated by FreeR set is different from an actual that of reflections because of the low resolution discrepancy. Moreover, twin refinement includes a problem about some overlapping of diffraction spot. But fortunately, I solved it. I just add twin tolerance parameter to refmac. In my case, parameters of L and H test slightly increase and the space group is P21, suggesting that it seems not to a merohedral twinning but a pseudo-merohedral twinning. In the case of pseudo merohedral, higher tolerance sometimes causes resolution stretch problem. After the refinement, resolution range and electron density map look fine so that I concluded that's the reason. Regards, Yuji To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] refmac twin refinement vs resolution limit
When I use refmac restrained refinement with intensity based twin refinement, resolution limit has been changed from 71.4519- 1.9996 to 47.392-1.986. From Log file, before refinement, Scaling and SigmaA resln: 71.4519 1.9996, seems to be correct. But in the middle of log file, around Fom and SigmaA vs resolution, Resolution limits are from 47.392 to 1.986. Output mtz file also shows different resolution range 71.46-1.925. Amplitude based twin refinement is also failed. Even if I input resolution range at Refinement parameters in refmac, the problem still occurs. I also read someone's similar problem, but it has been solved because of the update of refmac. What should I do to correspond with resolution range between the output of aimless and refmac restrained refinement? regards, Yuji To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
