[ccp4bb] Crystals with Organic solvents

[eswar reddy]

Dear All

I was working on a Human protein and expression and
solubility is good in E.coli  and purification is One step (His-Tag), and i
need to cleave the Histag before screens, if not
the protein will precipitated and Aggregated, but after trying for 1.2 years
i have crystals and they are with Organic solvents, (10 conditions), these
crystals are inter grown like *broccoli  *shaped  and i tried seeding, but
it is not successful, and even i tried  with additive screen but the result
is the same  is there is any idea to increase the size and shape of
my protein crystals.

Any suggestions will be helpful for me

Thanks in Advance

[ccp4bb] crystal Packing

[eswar reddy]

Dear All


I was working two domain protein and i have an hexagonal
look like crystals from  additive screen, now i am facing problem  these
crystal are growing in 2 minutes even in cold room even at lower protein
concentration @2mg/ml . and i have an smeary data of 7 Å, and we thinking
one domain might be flexible and other is stable

is there anyway  to increase the crystal packing 

  Any suggestions are welcome.

Eswar Reddy