[ccp4bb] 24-well sitting-drop plates

[Jacob Keller]

Dear Crystallogrpahers,

what are the best 24-well sitting drop plates on the market? I have used the 
standard pilar-type ones from Hampton, but I would like to have the ability 
to set up a few drops per well, and also those plates have a lot of 
background polarization. Also, the reason I am looking for 24-well plates is 
that I would like to set them up by hand, and 96 wells seems like a lot to 
do before sealing!


Best Regards,

Jacob Keller

***
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: j-kell...@northwestern.edu
***

Re: [ccp4bb] 24-well sitting-drop plates

[Martin Day]

Hello,

Emerald Biosystems inc. sell two types of sitting drop clover plates designed 
to be set up by hand. One of them combines 24 large wells (for the mother 
liquor) each surrounded by 4 small wells (for the protein drop), so there are 
96 
protien wells overall but you have fewer reservoirs to fill.

Martin

Re: [ccp4bb] 24-well sitting-drop plates

[David Briggs]

Sounds like you like to take a look at Art Robbins 24-4  (or is it 4-24?)
intelliplate. In the Hampton catalogue.

Hth

Dave
--
Delivered via an Android.

On Feb 24, 2010 12:20 AM, "Jacob Keller" 
wrote:

Dear Crystallogrpahers,

what are the best 24-well sitting drop plates on the market? I have used the
standard pilar-type ones from Hampton, but I would like to have the ability
to set up a few drops per well, and also those plates have a lot of
background polarization. Also, the reason I am looking for 24-well plates is
that I would like to set them up by hand, and 96 wells seems like a lot to
do before sealing!

Best Regards,

Jacob Keller

***
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: j-kell...@northwestern.edu
***

Re: [ccp4bb] 24-well sitting-drop plates

[Frank von Delft]

You don't *have* to use every single one of the 96 wells on a plate, of 
course -- it's quite feasible to use only 24 wells and then seal it.


If you buy that argument:  I recommend the 3-drop plates from SwissCi 
because you can do multiple drops per well, you can do pretty large 
volumes, and you don't need much reservoir volume (if you don't want to).


(Of course, you can also use the 2-drop MRC plates, but that's one less 
drop per well.)


phx



On 24/02/2010 10:07, David Briggs wrote:


Sounds like you like to take a look at Art Robbins 24-4  (or is it 
4-24?) intelliplate. In the Hampton catalogue.


Hth

Dave
--
Delivered via an Android.

On Feb 24, 2010 12:20 AM, "Jacob Keller" 
> wrote:


Dear Crystallogrpahers,

what are the best 24-well sitting drop plates on the market? I have 
used the standard pilar-type ones from Hampton, but I would like to 
have the ability to set up a few drops per well, and also those 
plates have a lot of background polarization. Also, the reason I am 
looking for 24-well plates is that I would like to set them up by 
hand, and 96 wells seems like a lot to do before sealing!


Best Regards,

Jacob Keller

***
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: j-kell...@northwestern.edu 
***

Re: [ccp4bb] 24-well sitting-drop plates

[mjvanraaij]

Dear Jacob,
I second the CompactClover plates suggested, either in 96-well (1 drop  
per well) or 24-well with four drops per well format. Easy to set up  
by hand, nice viewing characteristics, clear flat bottom of the drop.  
I think the polarisation is also ok, although I personally tend to  
take the polariser off the microscope to get more light - I don't feel  
polarisation adds much useful information anyway.
I don't find 96 wells is a lot of work before sealing and tend to use  
the broad sealing tape, but you can also buy the less broad sealing  
tape and seal the first half of the plate before continuing with the  
rest.
In Europe the CompactClover plates are also sold by Jena if I am not  
wrong, in Spain also by Diffractia - and in the USA (and rest of the  
world) by Emerald.

Greetings,
Mark

Mark J. van Raaij
http://webspersoais.usc.es/mark.vanraaij/
researcherID: B-3678-2009







On 24 Feb 2010, at 01:01, Jacob Keller wrote:


Dear Crystallogrpahers,

what are the best 24-well sitting drop plates on the market? I have  
used the standard pilar-type ones from Hampton, but I would like to  
have the ability to set up a few drops per well, and also those  
plates have a lot of background polarization. Also, the reason I am  
looking for 24-well plates is that I would like to set them up by  
hand, and 96 wells seems like a lot to do before sealing!


Best Regards,

Jacob Keller

***
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: j-kell...@northwestern.edu
***

Re: [ccp4bb] 24-well sitting-drop plates

[Patrick Shaw Stewart]

Jacob

There is the MRC Maxi, and also XtalQuest plate, both with 48 wells.
Less confusing for filling by hand than 96 wells


--
For information and discussion about protein crystallization and
automation, please join 
our bulletin board at
http://groups-beta.google.com/group/oryx_group?hl=en

 patr...@douglas.co.ukDouglas Instruments Ltd.
 DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK
 Directors: Peter Baldock, Patrick Shaw Stewart
 http://www.douglas.co.uk/
 Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034
 Regd. England 2177994, VAT Reg. GB 480 7371 36

> -Original Message-
> From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
> Jacob Keller
> Sent: 24 February 2010 00:02
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: [ccp4bb] 24-well sitting-drop plates
> 
> Dear Crystallogrpahers,
> 
> what are the best 24-well sitting drop plates on the market? I have
> used the
> standard pilar-type ones from Hampton, but I would like to have the
> ability
> to set up a few drops per well, and also those plates have a lot of
> background polarization. Also, the reason I am looking for 24-well
> plates is
> that I would like to set them up by hand, and 96 wells seems like a
lot
> to
> do before sealing!
> 
> Best Regards,
> 
> Jacob Keller
> 
> ***
> Jacob Pearson Keller
> Northwestern University
> Medical Scientist Training Program
> Dallos Laboratory
> F. Searle 1-240
> 2240 Campus Drive
> Evanston IL 60208
> lab: 847.491.2438
> cel: 773.608.9185
> email: j-kell...@northwestern.edu
> ***

Re: [ccp4bb] 24-well sitting-drop plates

[Patrick Shaw Stewart]

We have had dozens of boxes of XtalQuest 42-2 plates sitting on the
shelf for three years and I've just been told we've only sold three
boxes so far

We'll accept any reasonable offers!


> -Original Message-
> From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
> Patrick Shaw Stewart
> Sent: 25 February 2010 16:37
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] 24-well sitting-drop plates
> 
> Jacob
> 
> There is the MRC Maxi, and also XtalQuest plate, both with 48 wells.
> Less confusing for filling by hand than 96 wells
> 
> 
> --
> For information and discussion about protein crystallization and
> automation, please join
> our bulletin board at
> http://groups-beta.google.com/group/oryx_group?hl=en
> 
>  patr...@douglas.co.ukDouglas Instruments Ltd.
>  DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK
>  Directors: Peter Baldock, Patrick Shaw Stewart
>  http://www.douglas.co.uk/
>  Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034
>  Regd. England 2177994, VAT Reg. GB 480 7371 36
> 
> > -Original Message-
> > From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf
Of
> > Jacob Keller
> > Sent: 24 February 2010 00:02
> > To: CCP4BB@JISCMAIL.AC.UK
> > Subject: [ccp4bb] 24-well sitting-drop plates
> >
> > Dear Crystallogrpahers,
> >
> > what are the best 24-well sitting drop plates on the market? I have
> > used the
> > standard pilar-type ones from Hampton, but I would like to have the
> > ability
> > to set up a few drops per well, and also those plates have a lot of
> > background polarization. Also, the reason I am looking for 24-well
> > plates is
> > that I would like to set them up by hand, and 96 wells seems like a
> lot
> > to
> > do before sealing!
> >
> > Best Regards,
> >
> > Jacob Keller
> >
> > ***
> > Jacob Pearson Keller
> > Northwestern University
> > Medical Scientist Training Program
> > Dallos Laboratory
> > F. Searle 1-240
> > 2240 Campus Drive
> > Evanston IL 60208
> > lab: 847.491.2438
> > cel: 773.608.9185
> > email: j-kell...@northwestern.edu
> > ***