Re: [ccp4bb] Any suggestions?

[Dr. Isabel De Moraes]

Dear all,

My apologies for not being able to see the pictures. I have attached them as 
PDFs here but not sure if you will be able to get the files.

Anyway, many thanks to all who have reply via ccp4bb or privately with 
suggestions.  Indeed the protein is purified from the natural source so there 
are numerous possibilities.

Kind regards,
Isabel







On 7 Jul 2017, at 12:57, Dr. Isabel De Moraes 
> wrote:

Any suggestions regarding to the positive densities?

The crystallisation condition only has NaCl and CaCl



[cid:DD08D41F-CAB5-458D-BBD0-5DDCADC746FF@diamond.ac.uk]




[cid:B6BDEE5E-325C-4FEF-A7BC-B4F679D5315F@diamond.ac.uk]


Best regards,
Isabel




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-
Dr Isabel Moraes

Membrane Protein Laboratory  Group Leader

Diamond Light Source Ltd,
Harwell Science and Innovation Campus,
Oxfordshire, OX11 ODE, UK
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[cid:b2df3ed8-aa8e-4a06-a691-8732bdbf96ce@fed.cclrc.ac.uk]

Re: [ccp4bb] Any suggestions?

[Evans, Nicola]

Yes no images came through, however on looking at your crystallisation 
conditions for clues, is there anything in the protein prep that could have 
been carried through, even from the early stage buffers, or cell growth media? 
We once had a detergent in the cell lysis stage that was removed from all 
subsequent buffers (and protein put down 2 columns) but it made it through and 
produced density in the crystal structure.


Good luck with your search,


Nicola


From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Ian Clifton 
<ian.clif...@chem.ox.ac.uk>
Sent: 07 July 2017 14:23:36
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Any suggestions?

"Dr. Isabel  De Moraes" <isabel.de-mor...@diamond.ac.uk> writes:

> Any suggestions regarding to the positive densities?
>
Your pictures didn’t seem to make it as attachments, at least as
received here.

BW,
--
Ian ◎

Re: [ccp4bb] Any suggestions?

[Ian Clifton]

"Dr. Isabel  De Moraes"  writes:

> Any suggestions regarding to the positive densities?
>
Your pictures didn’t seem to make it as attachments, at least as
received here.

BW,
-- 
Ian ◎

[ccp4bb] Any suggestions?

[Dr. Isabel De Moraes]

Any suggestions regarding to the positive densities?

The crystallisation condition only has NaCl and CaCl



[cid:DD08D41F-CAB5-458D-BBD0-5DDCADC746FF@diamond.ac.uk]




[cid:B6BDEE5E-325C-4FEF-A7BC-B4F679D5315F@diamond.ac.uk]


Best regards,
Isabel




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This e-mail and any attachments may contain confidential, copyright and or 
privileged material, and are for the use of the intended addressee only. If you 
are not the intended addressee or an authorised recipient of the addressee 
please notify us of receipt by returning the e-mail and do not use, copy, 
retain, distribute or disclose the information in or attached to the e-mail.
Any opinions expressed within this e-mail are those of the individual and not 
necessarily of Diamond Light Source Ltd. 
Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments 
are free from viruses and we cannot accept liability for any damage which you 
may sustain as a result of software viruses which may be transmitted in or with 
the message.
Diamond Light Source Limited (company no. 4375679). Registered in England and 
Wales with its registered office at Diamond House, Harwell Science and 
Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom