[ccp4bb] Baculovirus expression system
Thank you every one for all your input. Our concern was whether there is any new advanced system than Bac-to-Bac or Bac-to-Bac is still pretty standard system for baculovirus expression. Based on all your inputs, it appears that we are not very outdated in our information about baculovirus expression system. There may be few newer systems but not much advanced than Bac-to-Bac and don't save significant amount of time and effort. Thank you Dhiraj To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
Re: [ccp4bb] Baculovirus expression system
There are several but if you discount the transfection based ones (i.e. direct DNA, no virus at all) then the speed is roughly the same - about four weeks from clone to process. FlashBac works a bit faster because it avoids the use of ecoli to recombine the bacmid but the time savings is about a week only. As to yield - it is very protein specific and you do get many variables in play including promoters, cell lines, media additives and so on Artem On Mon, Feb 7, 2022, 11:25 AM Srivastava, Dhiraj < dhiraj-srivast...@uiowa.edu> wrote: > Hi All > sorry for the question not related to crystallography. What is > the baculovirus expression system that people use these days to get good > yield in less time? > > Thank you > Dhiraj > > -- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 > To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
Re: [ccp4bb] Baculovirus expression system
Please follow the link https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/baculovirus-expression-system On Mon, 7 Feb 2022, 21:54 Srivastava, Dhiraj, wrote: > Hi All > sorry for the question not related to crystallography. What is > the baculovirus expression system that people use these days to get good > yield in less time? > > Thank you > Dhiraj > > -- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 > To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
Re: [ccp4bb] Baculovirus expression system
Dear Dhiraj, It may be perceived as “slow”, but we (still) use the Bac-to-Bac system from ThermoFisher (previously Invitrogen). This coupled with the pFastBac, MultiBac or biGBac vector systems generally works well; some proteins/complexes may express better in High 5 cells over Sf9/Sf21. With regards, Antony. Antony W Oliver FHEA, PhD Faculty Senior Research Fellow Genome Damage and Stability Centre Science Park Road University of Sussex Falmer, Brighton, BN1 9RQ (office): +44 (0)1273 678349 (lab): +44 (0)1273 677512 antony.oli...@sussex.ac.uk<mailto:antony.oli...@sussex.ac.uk>; (he/him) https://www.sussex.ac.uk/lifesci/oliverlab https://tinyurl.com/aw-oliver https://orcid.org/-0002-2912-8273 http://www.sussex.ac.uk/lifesci/internal/staff/support From: CCP4 bulletin board on behalf of "Srivastava, Dhiraj" Reply to: "Srivastava, Dhiraj" Date: Monday, 7 February 2022 at 16:24 To: "CCP4BB@JISCMAIL.AC.UK" Subject: [ccp4bb] Baculovirus expression system Hi All sorry for the question not related to crystallography. What is the baculovirus expression system that people use these days to get good yield in less time? Thank you Dhiraj To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] Baculovirus expression system
Hi All sorry for the question not related to crystallography. What is the baculovirus expression system that people use these days to get good yield in less time? Thank you Dhiraj To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
