[ccp4bb] Fusion proteins to solubilize your protein
I was wondering how many proteins that help to crystallize the protein of interest are out there... and how effective is the phase extension method (i.e. use MR to get the structure of the fusion protein into its density and the density of the protein of interest...) Is there a way to get this data from the PDB? Here is my list so far: - MBP - GFP - TRX - Lysozyme - GST Thanks To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
Re: [ccp4bb] Fusion proteins to solubilize your protein
Probably the easiest would be blasting sequences all by all and extracting peaks above certain height with a filter of some sorts. Not entirely trivial. I can add to your list, although not all of these proteins are in the PDB - some are from my own collection :) Lysozyme (s) several are used Artificial helix bundles GS SUMO Ubq NusA Fc and other mAb domains Nanobodies (as fusions) Cyt b562 Rubredoxin Flavodoxin VLR Barnase MHC proteins Bostjan Kobe wrote a paper in 2015 which you probably saw already. Artem On Tue, May 5, 2020, 2:45 PM Murpholino Peligro wrote: > I was wondering how many proteins that help to crystallize the protein of > interest are out there... > > and how effective is the phase extension method (i.e. use MR to get the > structure of the fusion protein into its density and the density of the > protein of interest...) > > Is there a way to get this data from the PDB? > > Here is my list so far: > - MBP > - GFP > - TRX > - Lysozyme > - GST > > Thanks > > > > > -- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
Re: [ccp4bb] Fusion proteins to solubilize your protein
Dear Murpholino, a portuguese company called NZYtech has a collection of fusion tag pHTP expression vectors (https://www.nzytech.com/wp-content/uploads/2019/04/nzyeasy-cloning-expression-system_userguide_v1901.pdf), some of them designed for increased protein's solubilization. We have a recent example of using a Ketosteroid isomerase (KSI) tag that highly increased solubility and crystallizability of one of our tagets (a kinase). No news on phasing and phase extension since crystals were not screened yet due to this long lock-down. HTH, best, Jose Subject Fusion proteins to solubilize your protein FromMurpholino Peligro DateTue 19:44 I was wondering how many proteins that help to crystallize the protein of interest are out there... and how effective is the phase extension method (i.e. use MR to get the structure of the fusion protein into its density and the density of the protein of interest...) Is there a way to get this data from the PDB? Here is my list so far: - MBP - GFP - TRX - Lysozyme - GST Thanks -- ** * Jose Artur Brito, PhD * * Research Fellow * * Membrane Protein Crystallography Laboratory * * Instituto de Tecnologia Quimica Biologica * * Avenida da Republica, EAN * * 2780-157 Oeiras * * PORTUGAL * ** * Telephone - +351 214 469 761 * * Mobile - +351 962 452 179 * * Fax - +351 214 433 644* ** * E-mail - jbr...@itqb.unl.pt * * Website - http://mx.itqb.unl.pt/ * ** To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
