[ccp4bb] In search of an article
I am looking for the reference of this little paper (in J. Appl. Cryst ?) describing how to make a loop and fish crystals... You know, this kind of humble method used by all crystallographers (apart FEL users). Thank you in advance Philippe Dumas
Re: [ccp4bb] In search of an article
Hi Phhilippe, T. Y. Teng, J. Appl. Crystallogr. 23, J. Appl. Crystallography 23:387–391 (1990) Mounting of crystals for macromolecular crystallography in a free-standing thin film A method for mounting single crystals in macromolecular crystallographic studies is described in which the crystal is suspended in a thin film. The film is formed from a mixture of the crystallization buffer and a hydrophilic viscous material, confined within a thin-wire loop by surface tension. Compared with conventional crystal mounting methods, this method greatly simplifies and speeds the mounting procedure, is well suited to shock freezing and to optical monitoring of the crystals, deforms fragile crystals less and gives a lower and more uniform background in the X-ray diffraction patterns. I do not have a copy, but if I remember well it was done with a copper wire, hair and nylon loops came later. Daniel Le 03/11/2014 18:23, DUMAS Philippe (VIE) a écrit : I am looking for the reference of this little paper (in J. Appl. Cryst ?) describing how to make a loop and fish crystals... You know, this kind of humble method used by all crystallographers (apart FEL users). Thank you in advance Philippe Dumas
Re: [ccp4bb] In search of an article
How appropriate that someone whose name is Picot would be the first one to have the answer. If you don't get it, put define:picot in a google search box. Frances = Bernstein + Sons * * Information Systems Consultants 5 Brewster Lane, Bellport, NY 11713-2803 * * *** *Frances C. Bernstein * *** f...@bernstein-plus-sons.com *** * * *** 1-631-286-1339FAX: 1-631-286-1999 = On Mon, 3 Nov 2014, Daniel Picot wrote: Hi Phhilippe, T. Y. Teng, J. Appl. Crystallogr. 23, J. Appl. Crystallography 23:387?391 (1990) Mounting of crystals for macromolecular crystallography in a free-standing thin film A method for mounting single crystals in macromolecular crystallographic studies is described in which the crystal is suspended in a thin film. The film is formed from a mixture of the crystallization buffer and a hydrophilic viscous material, confined within a thin-wire loop by surface tension. Compared with conventional crystal mounting methods, this method greatly simplifies and speeds the mounting procedure, is well suited to shock freezing and to optical monitoring of the crystals, deforms fragile crystals less and gives a lower and more uniform background in the X-ray diffraction patterns. I do not have a copy, but if I remember well it was done with a copper wire, hair and nylon loops came later. Daniel Le 03/11/2014 18:23, DUMAS Philippe (VIE) a ?crit : I am looking for the reference of this little paper (in J. Appl. Cryst ?) describing how to mak e a loop and fish crystals... You know, this kind of humble method used by all crystallographers (apart FEL users). Thank you in advance Philippe Dumas
Re: [ccp4bb] In search of an article
Le Lundi 3 Novembre 2014 19:06 CET, Frances C. Bernstein f...@bernstein-plus-sons.com a écrit: Thank you all ! I got it. Philippe D How appropriate that someone whose name is Picot would be the first one to have the answer. If you don't get it, put define:picot in a google search box. Frances = Bernstein + Sons * * Information Systems Consultants 5 Brewster Lane, Bellport, NY 11713-2803 * * *** *Frances C. Bernstein * *** f...@bernstein-plus-sons.com *** * * *** 1-631-286-1339FAX: 1-631-286-1999 = On Mon, 3 Nov 2014, Daniel Picot wrote: Hi Phhilippe, T. Y. Teng, J. Appl. Crystallogr. 23, J. Appl. Crystallography 23:387?391 (1990) Mounting of crystals for macromolecular crystallography in a free-standing thin film A method for mounting single crystals in macromolecular crystallographic studies is described in which the crystal is suspended in a thin film. The film is formed from a mixture of the crystallization buffer and a hydrophilic viscous material, confined within a thin-wire loop by surface tension. Compared with conventional crystal mounting methods, this method greatly simplifies and speeds the mounting procedure, is well suited to shock freezing and to optical monitoring of the crystals, deforms fragile crystals less and gives a lower and more uniform background in the X-ray diffraction patterns. I do not have a copy, but if I remember well it was done with a copper wire, hair and nylon loops came later. Daniel Le 03/11/2014 18:23, DUMAS Philippe (VIE) a ?crit : I am looking for the reference of this little paper (in J. Appl. Cryst ?) describing how to mak e a loop and fish crystals... You know, this kind of humble method used by all crystallographers (apart FEL users). Thank you in advance Philippe Dumas
Re: [ccp4bb] In search of an article
Hi Philippe, for the version with nylon fibres have a look at: Sauer and Ceska (1997) A simple method for making reproducible fibre loops for protein cryocrystallography, J. Appl. Cryst. 30, 71-72 regards, /Uwe On 2014-11-03 18:23, DUMAS Philippe (VIE) wrote: I am looking for the reference of this little paper (in J. Appl. Cryst ?) describing how to make a loop and fish crystals... You know, this kind of humble method used by all crystallographers (apart FEL users). Thank you in advance Philippe Dumas -- Dr. Uwe H. Sauer, Associate Professor BioCrystallography BioInformatics Group Computational Life Science Cluster, CLiC Umea Centre for Molecular Research, UCMR Centre for Chemical Biology, KBC Deptartment of Chemistry Umea University, Linnaeus vag 6, SE-901 87 Umea, Sweden Tel: +46-(0)90-786-5930 FAX: +46-(0)90-786-7655 e-mail: uwe.sa...@chem.umu.se URL: http://soul.ucmp.umu.se/~ucmp/uhs/
