Re: [ccp4bb] Off-topic x 2: Chaperone co-expression AND Bac-Mam contract work

[Michał Taube]

Hi,

You can find GroESL expressing plasmid and plasmids with different
chaperones combinations from prof. Bernd Bukau lab on Addgene:

https://www.addgene.org/Bernd_Bukau/

Best
Michal

2017-01-20 3:19 GMT+01:00 Patrick Loll :

> Hello everyone,
>
> Please allow me to kill two off-topic birds with one email ‘stone’:
>
> 1) Can anyone suggest where I can lay hands on the pREP4-GroESL plasmid
> for bacterial co-expression of GroEL and GroES? I’ve checked with the
> original author (Philip Cole), but it’s been decades since he published on
> this, and he’s not sure he can find the plasmid. However, I see recurrent
> (and recent) references to the plasmid in the literature, so I guess that
> it’s loose in the wild…
>
> 2) We’re looking for a contract research organization to do some Bac-Mam
> expression for us (we’re in a hurry to get some results, so we’d like to
> farm it out rather than get the technology going in-house). Can anyone
> recommend a group (ideally in the US, for logistical ease)?
>
> Thanks for any help.
>
> Cheers,
>
> Pat
>
>
>
> 
> ---
>
> Patrick J. Loll, Ph. D.
>
> Professor of Biochemistry & Molecular Biology
>
> Director, Biochemistry Graduate Program
>
> Drexel University College of Medicine
>
> Room 10-102 New College Building
>
> 245 N. 15th St., Mailstop 497
>
> Philadelphia, PA  19102-1192  USA
>
>
> (215) 762-7706
>
> pjl...@gmail.com
>
> pl...@drexelmed.edu
>
>

Re: [ccp4bb] Off-topic x 2: Chaperone co-expression AND Bac-Mam contract work

[Alexandra Deaconescu]

Hi Patrick:

We have used the Clonetech Chaperone Coexpression Kit. That includes  
GroEL/GroES plasmids.


http://www.clontech.com/BT/Products/Protein_Expression_and_Purification/Bacterial_Expression_Systems/Protein_Folding/Chaperone_Plasmid_Set


Hope this helps,

Alexandra




On 1/19/17 9:19 PM, Patrick Loll wrote:

Hello everyone,

Please allow me to kill two off-topic birds with one email ‘stone’:

1) Can anyone suggest where I can lay hands on the pREP4-GroESL 
plasmid for bacterial co-expression of GroEL and GroES? I’ve checked 
with the original author (Philip Cole), but it’s been decades since he 
published on this, and he’s not sure he can find the plasmid. However, 
I see recurrent (and recent) references to the plasmid in the 
literature, so I guess that it’s loose in the wild…


2) We’re looking for a contract research organization to do some 
Bac-Mam expression for us (we’re in a hurry to get some results, so 
we’d like to farm it out rather than get the technology going 
in-house). Can anyone recommend a group (ideally in the US, for 
logistical ease)?


Thanks for any help.

Cheers,

Pat



---

Patrick J. Loll, Ph. D.

Professor of Biochemistry & Molecular Biology

Director, Biochemistry Graduate Program

Drexel University College of Medicine

Room 10-102 New College Building

245 N. 15th St., Mailstop 497

Philadelphia, PA19102-1192USA


(215) 762-7706

pjl...@gmail.com 

pl...@drexelmed.edu 




--
Alexandra M. Deaconescu, B.E., Ph.D.
Assistant Professor of Molecular Biology, Cell Biology and Biochemistry

[ccp4bb] Off-topic x 2: Chaperone co-expression AND Bac-Mam contract work

[Patrick Loll]

Hello everyone,

Please allow me to kill two off-topic birds with one email ‘stone’:  

1) Can anyone suggest where I can lay hands on the pREP4-GroESL plasmid for 
bacterial co-expression of GroEL and GroES? I’ve checked with the original 
author (Philip Cole), but it’s been decades since he published on this, and 
he’s not sure he can find the plasmid. However, I see recurrent (and recent) 
references to the plasmid in the literature, so I guess that it’s loose in the 
wild…

2) We’re looking for a contract research organization to do some Bac-Mam 
expression for us (we’re in a hurry to get some results, so we’d like to farm 
it out rather than get the technology going in-house). Can anyone recommend a 
group (ideally in the US, for logistical ease)?

Thanks for any help.

Cheers,

Pat



---
Patrick J. Loll, Ph. D.  
Professor of Biochemistry & Molecular Biology
Director, Biochemistry Graduate Program
Drexel University College of Medicine
Room 10-102 New College Building
245 N. 15th St., Mailstop 497
Philadelphia, PA  19102-1192  USA

(215) 762-7706
pjl...@gmail.com
pl...@drexelmed.edu