Dear All, thanks very much for the various suggestions--I finally made the
connection. A good point was also made that gene synthesis is becoming ever
cheaper (~$0.28/bp) so maybe PCRing from genomic DNA is not always
advisable. I'll keep this in mind for the future, although for this one I
had my reasons for going for the gDNA.
Thanks again,
Jacob
On Tue, Mar 5, 2013 at 1:07 PM, Jacob Keller j-kell...@fsm.northwestern.edu
wrote:
Dear List,
please pardon the major off-topicity, but I need a trace amount of V
cholera genomic DNA to PCR from, and it just kills me to spend ~$300 to
order it for this one and only use. Is anyone in the DC/Baltimore area
willing and able to donate this? I travel on the capitol beltway daily, so
anything nearby would work, esp. NIH.
Jacob
--
***
Jacob Pearson Keller, PhD
Postdoctoral Associate
HHMI Janelia Farms Research Campus
email: k j-kell...@northwestern.eduell...@janelia.hhmi.org
***
--
***
Jacob Pearson Keller, PhD
Postdoctoral Associate
HHMI Janelia Farms Research Campus
email: k j-kell...@northwestern.eduell...@janelia.hhmi.org
***