Re: [ccp4bb] long loop

[mesters]

Do you have an inactive variant of the protein? If yes, use limited 
proteolysis in the presence of methylated histones to find out which 
flexible parts are really important and or protected by complex 
formation. Further, try to crystallize a complex of inactive demethylase 
and methylated histones, a long shot but a good way to combat the 
apparent flexibility


Good luck,

Jeroen


Am 04.07.17 um 17:22 schrieb dongxiaofei:

Dear ALL,
I want to make a protein crystal,but there is a long loop between 
domains of protein , which contains two small domains owning about 40 
amino acids respectively and a loop about 70 amino acids.
Loop is so long and flexible ,but I don't want to delete some 
fragments,because it may be  important for protein's function of a 
histone demethylase.

Besides, the  surface charge of  protein is whole negative .
I have tried a long time but it is hard to me to get crystal.

Would be very grateful for any advice!

Thanks

Dong Xiao






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Re: [ccp4bb] long loop

[Briggs, David C]

I know that this might be considered heresy on a crystallography mailing list, 
but do you have any friendly NMRists at your institution?


D


Dr David C Briggs

Hohenester Lab

Department of Life Sciences

Imperial College London

UK

http://about.me/david_briggs


From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of dongxiaofei 
<18811070...@163.com>
Sent: 04 July 2017 16:22:39
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] long loop

Dear ALL,
I want to make a protein crystal,but there is a long loop between domains of 
protein , which contains two small domains owning about 40 amino acids 
respectively and a loop about 70 amino acids.
Loop is so long and flexible ,but I don't want to delete some fragments,because 
it may be  important for protein's function of a histone demethylase.
Besides, the  surface charge of  protein is whole negative .
I have tried a long time but it is hard to me to get crystal.

Would be very grateful for any advice!
Thanks
Dong Xiao

Re: [ccp4bb] long loop

[Smith Liu]

Fab fragment binding towards long loop



在2017年07月04日 23:22，dongxiaofei 写道：
Dear ALL,
I want to make a protein crystal,but there is a long loop between domains of 
protein , which contains two small domains owning about 40 amino acids 
respectively and a loop about 70 amino acids.
Loop is so long and flexible ,but I don't want to delete some fragments,because 
it may be  important for protein's function of a histone demethylase.
Besides, the  surface charge of  protein is whole negative .
I have tried a long time but it is hard to me to get crystal.
 

Would be very grateful for any advice!

Thanks

Dong Xiao

 

 





 

[ccp4bb] long loop

[dongxiaofei]

Dear ALL,
I want to make a protein crystal,but there is a long loop between domains of 
protein , which contains two small domains owning about 40 amino acids 
respectively and a loop about 70 amino acids.
Loop is so long and flexible ,but I don't want to delete some fragments,because 
it may be  important for protein's function of a histone demethylase.
Besides, the  surface charge of  protein is whole negative .
I have tried a long time but it is hard to me to get crystal.
 

Would be very grateful for any advice!

Thanks

Dong Xiao