[Freesurfer] Surface area intepretation problem

2011-02-02 Thread Jürgen Hänggi
Dear FS experts

We divided the superior temporal sulcus derived from the aparc.a2009s.annot
into parcellations of 300mm2 (using mris_divide_parcellation) and computed
the mean cortical thickness within each parcellation. So far so good.

But we noticed that each parcellation has a unique surface area that
deviates largely from 300mm2 (96-480mm2).

We defined the parcells on the fsaverage template and transformed them back
into the native space of each individual. Therefore, we expected some
variation in the surface area, but not such large differences.

How to explain it?

Thanks in advance
Regards
Jürgen


Jürgen Hänggi, Ph.D.
Division Neuropsychology
Institute of Psychology
University of Zurich
Binzmuehlestrasse 14, PO Box 25
8050 Zurich, Switzerland
0041 44 635 73 97 (phone office)
0041 76 445 86 84 (phone mobile)
0041 44 635 74 09 (fax office)
BIN 4.D.04 (office room number)
j.haenggi[at]psychologie.uzh.ch (email)
http://www.psychologie.uzh.ch/neuropsy/ (website)
http://www.juergenhaenggi.ch (private website)

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Re: [Freesurfer] Segmentation problems

2011-02-02 Thread Tetiana Dadakova
Dear Bruce and all,

I am trying to fix the scull strip, as it affects ?h.white/?h.pial
surfaces. I used -wsthresh with preflooding height of down to 1. It
didn't really help much. Therefore, I have two question:

1. Regarding mri_gcut: should I use the default intensity of 110 for
white matter mask? How much it is acceptable to change the -T
parameter (threshold to value of WM intensity), which default value is
0.36?

2. Regarding manual removing of some voxels: the 'Edit Voxels' tool
doesn't seem to work on MacOS. Even though I have a 3-button mouse,
when I try click (or click and drag) with any mouse button, nothing
happens. What can be wrong?

Thank you,
Tetiana.


On Mon, Jan 31, 2011 at 2:00 PM, Bruce Fischl
fis...@nmr.mgh.harvard.edu wrote:
 Hi Tetiana,

 do you mean the bit of skull left over that is misclassified? I wouldn't
 worry about it unless it causes the ?h.white/?h.pial surfaces to be
 inaccurate. In general we use the surface-based measures to estimate
 gray/white morphometry and the aseg for everything else. It is a minor skull
 strip failure and you could either fix it by manually erasing it from the
 brainmask.mgz or trying to mess with the watershed/gcut.

 cheers
 Bruce


 On Mon, 31 Jan 2011, Tetiana Dadakova wrote:

 Dear FreeSurfer users,

 I run a recon-all -all for one of my subjects. When I check the
 segmentation, it looks incorrect (see attached picture).
 What can be the problem? At which step can I change something? Should
 I do manual correction?

 Thank you,
 Tetiana.



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Re: [Freesurfer] Segmentation problems

2011-02-02 Thread Nick Schmansky
try disabling use of gcut by adding -no-gcut to the end of recon-all.
sometimes it can cut too much (something thats fixed in the upcoming
release).

n.

On Wed, 2011-02-02 at 11:28 +0100, Tetiana Dadakova wrote:
 Dear Bruce and all,
 
 I am trying to fix the scull strip, as it affects ?h.white/?h.pial
 surfaces. I used -wsthresh with preflooding height of down to 1. It
 didn't really help much. Therefore, I have two question:
 
 1. Regarding mri_gcut: should I use the default intensity of 110 for
 white matter mask? How much it is acceptable to change the -T
 parameter (threshold to value of WM intensity), which default value is
 0.36?
 
 2. Regarding manual removing of some voxels: the 'Edit Voxels' tool
 doesn't seem to work on MacOS. Even though I have a 3-button mouse,
 when I try click (or click and drag) with any mouse button, nothing
 happens. What can be wrong?
 
 Thank you,
 Tetiana.
 
 
 On Mon, Jan 31, 2011 at 2:00 PM, Bruce Fischl
 fis...@nmr.mgh.harvard.edu wrote:
  Hi Tetiana,
 
  do you mean the bit of skull left over that is misclassified? I wouldn't
  worry about it unless it causes the ?h.white/?h.pial surfaces to be
  inaccurate. In general we use the surface-based measures to estimate
  gray/white morphometry and the aseg for everything else. It is a minor skull
  strip failure and you could either fix it by manually erasing it from the
  brainmask.mgz or trying to mess with the watershed/gcut.
 
  cheers
  Bruce
 
 
  On Mon, 31 Jan 2011, Tetiana Dadakova wrote:
 
  Dear FreeSurfer users,
 
  I run a recon-all -all for one of my subjects. When I check the
  segmentation, it looks incorrect (see attached picture).
  What can be the problem? At which step can I change something? Should
  I do manual correction?
 
  Thank you,
  Tetiana.
 
 
 
  The information in this e-mail is intended only for the person to whom it is
  addressed. If you believe this e-mail was sent to you in error and the
  e-mail
  contains patient information, please contact the Partners Compliance
  HelpLine at
  http://www.partners.org/complianceline . If the e-mail was sent to you in
  error
  but does not contain patient information, please contact the sender and
  properly
  dispose of the e-mail.
 
 
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[Freesurfer] Am I losing T2* info in MEMPRAGE data by differential scaling?

2011-02-02 Thread Carl Anderson
Dear Freesurfer,

I am concerned that I may not be processing the MEMPRAGE images to full take
advantage of the T2* info in the multi-echos.

I have been separating each echo as 001.mgz, 002.mgz, 003.mgz  004.mgz when
using recon-all.  This works well if motion exists in any of the individual
echos.

However of late, I have noticed a that a reference was made to the problem
of losing T2* info by differential scaling in the archive if a particular
file format memprage_echo0.mgz was not used.

Here is the link to the archive note:
http://www.mail-archive.com/freesurfer@nmr.mgh.harvard.edu/msg15303.html

It has been hard to identify a difference between our standard MPRAGE images
and the MEMPRAGE images (we currently collect both on our Siemens 3T 32
channel system).

Am I processing the MEMPRAGE correctly?

Thanks!

-- 
•~~•~~•~~•~~•~~•~~•~~•
Carl M. Anderson, Ph.D. (mail stop 204)
Assistant Professor of Psychiatry,
Harvard Medical School 
The Neuroimaging Center,
McLean Hospital 115 Mill St.  Belmont, MA 02478
Ph: 617-855-2972;  Fax: 617-855-2770
e-mail: remfr...@gmail.com; carl_ander...@hms.harvard.edu
•~~•~~•~~•~~•~~•~~•~~•
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[Freesurfer] Problem running recon-all with option -qcache

2011-02-02 Thread Ed Gronenschild
Hi,

A serious error was encountered in running recon-all with option
-qcache.
The error occurred during execution of mri_surf2surf and the
message was:

number of vertices in ../subjectID/surf/lh.thickness does not match
surface (141969,131763).

It means the number of vertices in lh.sphere.reg does not
match the number in lh.thickness.

I traced the course of this error and that is causing me serious
troubles. What happened? I edited the wm voxels and then
invoked another call to recon-all by issueing the command
recon-all -autorecon2-wm.
Looking at the dates of the files in the surf directory I noticed
that the file lh.sphere.reg was not updated, so it was still the
same file as before editing the wm voxels. The lh.thickness
was correctly updated as expected.

What can I do to use the -qcache option?

Ed

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[Freesurfer] testing FreeSurfer

2011-02-02 Thread Carolina Valencia
Hi FreeSurfer's user,
I'm new using this software, I try to install on Ubuntu and to set up
following the recommendation of the webpage.
Then I tried to test but any commmand seems to be recognized, also when I
started a new terminal a warning is shown:

 freesurfer-Linux-centos4_x86_64-stable-pub-v5.0.0 
Setting up environment for FreeSurfer/FS-FAST (and FSL)
WARNING: /home/cvalencia/Descargas/freesurfer/fsfast does not exist
FREESURFER_HOME   /home/cvalencia/Descargas/freesurfer
FSFAST_HOME   /home/cvalencia/Descargas/freesurfer/fsfast
FSF_OUTPUT_FORMAT nii
SUBJECTS_DIR  /home/cvalencia/Descargas/freesurfer/subjects
MNI_DIR   /home/cvalencia/Descargas/freesurfer/mni
FSL_DIR   /usr/share/fsl/4.1


Thanks in advance for the support,

Best regards,

cvalencia
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Re: [Freesurfer] Segmentation problems

2011-02-02 Thread Bruce Fischl
This is probably ok. Run it all the way through and see if it affects the 
surfaces. If not, you are all set


On Feb 2, 2011, at 10:30 AM, Tetiana Dadakova tetian...@gmail.com wrote:

 I attach a picture of what I get if I run
 
 recon-all -autorecon1 -subjid name_of_subj -no-gcut.
 
 I feel that I need to change the -T threshold of WM intensity, does it
 make sense? If I put this -T value to 0.5, the out-of-brain structures
 are cut, but I wonder how it affects the white matter.
 
 Thank you,
 Tanja
 
 
 On Wed, Feb 2, 2011 at 2:36 PM, Nick Schmansky
 ni...@nmr.mgh.harvard.edu wrote:
 try disabling use of gcut by adding -no-gcut to the end of recon-all.
 sometimes it can cut too much (something thats fixed in the upcoming
 release).
 
 n.
 
 On Wed, 2011-02-02 at 11:28 +0100, Tetiana Dadakova wrote:
 Dear Bruce and all,
 
 I am trying to fix the scull strip, as it affects ?h.white/?h.pial
 surfaces. I used -wsthresh with preflooding height of down to 1. It
 didn't really help much. Therefore, I have two question:
 
 1. Regarding mri_gcut: should I use the default intensity of 110 for
 white matter mask? How much it is acceptable to change the -T
 parameter (threshold to value of WM intensity), which default value is
 0.36?
 
 2. Regarding manual removing of some voxels: the 'Edit Voxels' tool
 doesn't seem to work on MacOS. Even though I have a 3-button mouse,
 when I try click (or click and drag) with any mouse button, nothing
 happens. What can be wrong?
 
 Thank you,
 Tetiana.
 
 
 On Mon, Jan 31, 2011 at 2:00 PM, Bruce Fischl
 fis...@nmr.mgh.harvard.edu wrote:
 Hi Tetiana,
 
 do you mean the bit of skull left over that is misclassified? I wouldn't
 worry about it unless it causes the ?h.white/?h.pial surfaces to be
 inaccurate. In general we use the surface-based measures to estimate
 gray/white morphometry and the aseg for everything else. It is a minor 
 skull
 strip failure and you could either fix it by manually erasing it from the
 brainmask.mgz or trying to mess with the watershed/gcut.
 
 cheers
 Bruce
 
 
 On Mon, 31 Jan 2011, Tetiana Dadakova wrote:
 
 Dear FreeSurfer users,
 
 I run a recon-all -all for one of my subjects. When I check the
 segmentation, it looks incorrect (see attached picture).
 What can be the problem? At which step can I change something? Should
 I do manual correction?
 
 Thank you,
 Tetiana.
 
 
 
 The information in this e-mail is intended only for the person to whom it 
 is
 addressed. If you believe this e-mail was sent to you in error and the
 e-mail
 contains patient information, please contact the Partners Compliance
 HelpLine at
 http://www.partners.org/complianceline . If the e-mail was sent to you in
 error
 but does not contain patient information, please contact the sender and
 properly
 dispose of the e-mail.
 
 
 ___
 Freesurfer mailing list
 Freesurfer@nmr.mgh.harvard.edu
 https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer
 
 
 
 
 skull_strip.tiff

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Re: [Freesurfer] Am I losing T2* info in MEMPRAGE data by differential scaling?

2011-02-02 Thread Douglas N Greve
Hi Carl, you're probably better off taking the RMS (root-mean-square) of 
the echoes rather than averaging them together. Our scanner outputs 
these in addition to the echoes, but they are easy to compute with calls 
to mri_concat.

doug

btw, you can't really have motion between the echoes. All four echoes 
for each line of k-space are acquired within 20ms of each other.

Carl Anderson wrote:
 Dear Freesurfer,

 I am concerned that I may not be processing the MEMPRAGE images to 
 full take advantage of the T2* info in the multi-echos.

 I have been separating each echo as 001.mgz, 002.mgz, 003.mgz  
 004.mgz when using recon-all.  This works well if motion exists in any 
 of the individual echos.

 However of late, I have noticed a that a reference was made to the 
 problem of losing T2* info by differential scaling in the archive if a 
 particular file format memprage_echo0.mgz was not used.

 Here is the link to the archive note: 
 http://www.mail-archive.com/freesurfer@nmr.mgh.harvard.edu/msg15303.html

 It has been hard to identify a difference between our standard MPRAGE 
 images and the MEMPRAGE images (we currently collect both on our 
 Siemens 3T 32 channel system).

 Am I processing the MEMPRAGE correctly?

 Thanks!

 -- 
 •~~•~~•~~•~~•~~•~~•~~•
 Carl M. Anderson, Ph.D. (mail stop 204)
 Assistant Professor of Psychiatry,
 Harvard Medical School 
 The Neuroimaging Center,
 McLean Hospital 115 Mill St.  Belmont, MA 02478
 Ph: 617-855-2972;  Fax: 617-855-2770
 e-mail: remfr...@gmail.com mailto:remfr...@gmail.com; 
 carl_ander...@hms.harvard.edu mailto:carl_ander...@hms.harvard.edu
 •~~•~~•~~•~~•~~•~~•~~•

 

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-- 
Douglas N. Greve, Ph.D.
MGH-NMR Center
gr...@nmr.mgh.harvard.edu
Phone Number: 617-724-2358 
Fax: 617-726-7422

Bugs: surfer.nmr.mgh.harvard.edu/fswiki/BugReporting
FileDrop: www.nmr.mgh.harvard.edu/facility/filedrop/index.html

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Re: [Freesurfer] Problem running recon-all with option -qcache

2011-02-02 Thread Douglas N Greve
Hi Ed, that subject is out-of-synch (maybe it got aborted). You'll need 
to re-run some portion of recon-all depending upon where in the stream 
it aborted.

doug

Ed Gronenschild wrote:
 Hi,

 A serious error was encountered in running recon-all with option
 -qcache.
 The error occurred during execution of mri_surf2surf and the
 message was:

 number of vertices in ../subjectID/surf/lh.thickness does not match
 surface (141969,131763).

 It means the number of vertices in lh.sphere.reg does not
 match the number in lh.thickness.

 I traced the course of this error and that is causing me serious
 troubles. What happened? I edited the wm voxels and then
 invoked another call to recon-all by issueing the command
 recon-all -autorecon2-wm.
 Looking at the dates of the files in the surf directory I noticed
 that the file lh.sphere.reg was not updated, so it was still the
 same file as before editing the wm voxels. The lh.thickness
 was correctly updated as expected.

 What can I do to use the -qcache option?

 Ed

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-- 
Douglas N. Greve, Ph.D.
MGH-NMR Center
gr...@nmr.mgh.harvard.edu
Phone Number: 617-724-2358 
Fax: 617-726-7422

Bugs: surfer.nmr.mgh.harvard.edu/fswiki/BugReporting
FileDrop: www.nmr.mgh.harvard.edu/facility/filedrop/index.html

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[Freesurfer] One Way Anova and multiple comparisons

2011-02-02 Thread marco battaglini

Hi to everybody, 
 I am a very inexperienced users of FreeSurfer. I have 3 groups of subjects
and I have run recon-all and checked the results for all the subjects (very 
good results).

Now what I need to do, is to calculate the differences in the regional cortical 
thickness between these
3 groups (using age and gender as covariates) and to perform multiple 
comparisons, in order to detect
possible local differences in cortical thickness.

Is it possible to perform this kind of analysis also for comparing surfaces 
between different groups?

Could you give me a short pipeline about the steps I need to do, or redirect 
me
to some focused documentation?

Thank's.

Marco


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Re: [Freesurfer] One Way Anova and multiple comparisons

2011-02-02 Thread Douglas N Greve
Yes, you can do these types of things. You'll need to create an FSGD 
file. Search for FSGD on the wiki and go to the examples page, there's 
not an example of 3 groups and 2 covariates, but there are enough 
examples there that you can piece it together. After you have your FSGD 
file, run mris_preproc, mri_surf2surf to smooth, mri_glmfit, then 
mri_glmfit-sim to correct for multiple comparisons. There's a group 
analysis tutorial on the website too.

doug

marco battaglini wrote:
 Hi to everybody,
  I am a very inexperienced users of FreeSurfer. I have 3 groups of 
 subjects
 and I have run recon-all and checked the results for all the subjects 
 (very good results).

 Now what I need to do, is to calculate the differences in the regional 
 cortical thickness between these
 3 groups (using age and gender as covariates) and to perform multiple 
 comparisons, in order to detect
 possible local differences in cortical thickness.

 Is it possible to perform this kind of analysis also for comparing 
 surfaces between different groups?

 Could you give me a short pipeline about the steps I need to do, or 
 redirect me
 to some focused documentation?

 Thank's.

 Marco


 

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-- 
Douglas N. Greve, Ph.D.
MGH-NMR Center
gr...@nmr.mgh.harvard.edu
Phone Number: 617-724-2358 
Fax: 617-726-7422

Bugs: surfer.nmr.mgh.harvard.edu/fswiki/BugReporting
FileDrop: www.nmr.mgh.harvard.edu/facility/filedrop/index.html

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[Freesurfer] error during autorecon2-wm routine

2011-02-02 Thread Larissa Stanberry
Dear experts,

after correcting WM, I got the following error running recon-all - 
autorecon-wm -subjid XXX

ERROR: _FindFacePath: could not find path!

It happens in the middle of the correcting topology routine. I'd  
appreciate any input on as to why it happens and how to fix it.

Cheers
Larissa

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Re: [Freesurfer] transform to original mri

2011-02-02 Thread Douglas N Greve
you'll have to first create a registration file, you can do that with

cd subject/mri
tkregister2 --mov orig/001.mgz --targ T1.mgz --regheader --s subject 
--reg register.dat --noedit

then apply this to the surface with

mri_surf2surf --s $subject --sval-xyz white --hemi lh --reg register.dat 
orig/001.mgz \
   --tval-xyz --tval ./lh.white.001

This creates lh.white.001, and the coordinate will/should be in the 
tkregister coorindates of the 001.mgz space (usually they are in the 
tkregister coords of the T1.mgz space). See
https://surfer.nmr.mgh.harvard.edu/fswiki/CoordinateSystems?action=AttachFiledo=gettarget=fscoordinates.ppt
for how to get from this space to other spaces.

doug



Seok Lew wrote:
 Dear all,

 I am trying to transform the reconstructed surface like rh.pial into  
 the original mri's (like 001.mgz) coordinate. I found some matrices in  
 the ./transform directory, but not sure of which one (or a set of  
 matrices) would do the transform?

 Seok
 s...@nmr.mgh.harvard.edu




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gr...@nmr.mgh.harvard.edu
Phone Number: 617-724-2358 
Fax: 617-726-7422

Bugs: surfer.nmr.mgh.harvard.edu/fswiki/BugReporting
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Re: [Freesurfer] using mri_glmfit-sim

2011-02-02 Thread Douglas N Greve
These are two distinct methods of correcting. One, is to synthesize, 
smooth, analyze, and cluster. The second is to permute, analyze, and 
cluster. These are realized by two different arguments to the --sim flag 
in mri_glmfit-sim (either --sim mc-z ... or --sim perm ...)

doug

Chris Hyatt wrote:
 I've read both Hagler et al 2006 Neuroimage (Smoothing and cluster 
 thresholding for cortical surface-based group analysis of fMRI data) 
 and B. Douglas Ward's description of AlphaSim that both describe 
 clusterwise correction for multiple comparisons.

 I recently found, however, an MGH Freesurfer presentation that 
 mentioned that after synthesizing Gaussian noise and smoothing (Monte 
 Carlo), the rows of the design matrix were permuted.

 Does the mri_glmfit-sim clusterwise correction method permute rows of 
 the design matrix? I'm still a bit confused about the exact method the 
 clusterwise correction for multiple comparisons work in Freesurfer.

 Thanks for your help.

 Christopher Hyatt, PhD
 IOL/Olin Neuropsychiatry Research Center
 Hartford, CT
 

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Re: [Freesurfer] Am I losing T2* info in MEMPRAGE data by differential scaling?

2011-02-02 Thread Carl Anderson
Well our primary reason for using this sequence is to better delineate the
amygdala.

On Wed, Feb 2, 2011 at 5:03 PM, Bruce Fischl fis...@nmr.mgh.harvard.eduwrote:

 what do you want to do with the echoes?

 On Wed, 2 Feb 2011, Douglas N Greve wrote:

  Hi Carl, you're probably better off taking the RMS (root-mean-square) of
 the echoes rather than averaging them together. Our scanner outputs
 these in addition to the echoes, but they are easy to compute with calls
 to mri_concat.

 doug

 btw, you can't really have motion between the echoes. All four echoes
 for each line of k-space are acquired within 20ms of each other.

 Carl Anderson wrote:

 Dear Freesurfer,

 I am concerned that I may not be processing the MEMPRAGE images to
 full take advantage of the T2* info in the multi-echos.

 I have been separating each echo as 001.mgz, 002.mgz, 003.mgz 
 004.mgz when using recon-all.  This works well if motion exists in any
 of the individual echos.

 However of late, I have noticed a that a reference was made to the
 problem of losing T2* info by differential scaling in the archive if a
 particular file format memprage_echo0.mgz was not used.

 Here is the link to the archive note:
 http://www.mail-archive.com/freesurfer@nmr.mgh.harvard.edu/msg15303.html

 It has been hard to identify a difference between our standard MPRAGE
 images and the MEMPRAGE images (we currently collect both on our
 Siemens 3T 32 channel system).

 Am I processing the MEMPRAGE correctly?

 Thanks!

 --

 ÿÿ~~ÿÿ~~ÿÿ~~ÿÿ~~ÿÿ~~ÿÿ~~ÿÿ~~ÿÿ

 Carl M. Anderson, Ph.D. (mail stop 204)
 Assistant Professor of Psychiatry,
 Harvard Medical School 
 The Neuroimaging Center,
 McLean Hospital 115 Mill St.  Belmont, MA 02478
 Ph: 617-855-2972;  Fax: 617-855-2770
 e-mail: remfr...@gmail.com mailto:remfr...@gmail.com;
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Assistant Professor of Psychiatry,
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The Neuroimaging Center,
McLean Hospital 115 Mill St.  Belmont, MA 02478
Ph: 617-855-2972;  Fax: 617-855-2770
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[Freesurfer] Surface area interpretation confusion

2011-02-02 Thread Jürgen Hänggi
Dear FS experts

Sorry, for reposting this question, but I'am not sure whether something went
wrong.

We divided the superior temporal sulcus derived from the aparc.a2009s.annot
into parcellations of 300mm2 in size (using mris_divide_parcellation) and
computed the mean cortical thickness within each parcellation.

But after running mris_anatomical-stats, we noticed that each parcellation
has a unique surface area that deviates largely from 300mm2 (ranging between
96mm2 to 480mm2).

Does anybody have any ideas?
Is this a bug or is this what one should expect?

We defined the parcells on the fsaverage template and transformed them back
into the native space of each individual. Therefore, we expected some
variation in the surface area, but not such large differences.

Thanks in advance
Regards
Jürgen



Jürgen Hänggi, Ph.D.
Division Neuropsychology
Institute of Psychology
University of Zurich
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