Re: [Freesurfer] Xhemi: vertex-wise volume asymmetry analysis?

2014-04-28 Thread Yizhou Ma
Thanks Doug. I have ?h.volume in subject/surf but not in
subject/xhemi/surf. I followed this page
http://surfer.nmr.mgh.harvard.edu/fswiki/Xhemi and I think it is a problem
with surfreg rather than mris_preproc. Can I just copy the ?h.volume from
subject/surf to subject/xhemi/surf?

Best,
Cherry


On Mon, Apr 28, 2014 at 4:01 PM, Douglas N Greve
gr...@nmr.mgh.harvard.eduwrote:



 There should be a ?h.volume file. Are you sure there is not? You can
 specify -meas volume when you run mris_preproc
 doug

 On 04/27/2014 08:35 PM, Yizhou Ma wrote:
  Dear freesurfer experts,
 
  I am working on a project on cortical asymmetry and I have analyzed
  vertex-wise cortical thickness/surface area asymmetry. However, I
  noticed that Freesurfer doesn't not seem to facilitate such analysis
  for gray matter volume, as after running surfreg -xhemi there is no
  ?h.volume file in subject/xhemi/surf. I searched the literature and
  found studies on gray matter density asymmetry with VBM and ROI-based
  volume asymmetry. Is it true that Freesurfer doesn't offer vertex-wise
  volume asymmetry analysis? And may I know the rationale?
 
  Thanks,
  Cherry
 
 
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 gr...@nmr.mgh.harvard.edu
 Phone Number: 617-724-2358
 Fax: 617-726-7422

 Bugs: surfer.nmr.mgh.harvard.edu/fswiki/BugReporting
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Re: [Freesurfer] Xhemi: vertex-wise volume asymmetry analysis?

2014-04-28 Thread Yizhou Ma
Thanks Doug. I don't see an error message in xhemireg.?h.log files. Please
see attached for the files.


On Mon, Apr 28, 2014 at 4:22 PM, Douglas N Greve
gr...@nmr.mgh.harvard.eduwrote:


 Then something went wrong along the way. Check the xhemi/xhemireg.?h.log
 to see if there is an error. If not, send the log file to me

 On 04/28/2014 04:14 PM, Yizhou Ma wrote:
  Thanks Doug. I have ?h.volume in subject/surf but not in
  subject/xhemi/surf. I followed this page
  http://surfer.nmr.mgh.harvard.edu/fswiki/Xhemi and I think it is a
  problem with surfreg rather than mris_preproc. Can I just copy the
  ?h.volume from subject/surf to subject/xhemi/surf?
 
  Best,
  Cherry
 
 
  On Mon, Apr 28, 2014 at 4:01 PM, Douglas N Greve
  gr...@nmr.mgh.harvard.edu mailto:gr...@nmr.mgh.harvard.edu wrote:
 
 
 
  There should be a ?h.volume file. Are you sure there is not? You can
  specify -meas volume when you run mris_preproc
  doug
 
  On 04/27/2014 08:35 PM, Yizhou Ma wrote:
   Dear freesurfer experts,
  
   I am working on a project on cortical asymmetry and I have analyzed
   vertex-wise cortical thickness/surface area asymmetry. However, I
   noticed that Freesurfer doesn't not seem to facilitate such
 analysis
   for gray matter volume, as after running surfreg -xhemi there is no
   ?h.volume file in subject/xhemi/surf. I searched the literature and
   found studies on gray matter density asymmetry with VBM and
  ROI-based
   volume asymmetry. Is it true that Freesurfer doesn't offer
  vertex-wise
   volume asymmetry analysis? And may I know the rationale?
  
   Thanks,
   Cherry
  
  
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  --
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  MGH-NMR Center
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  Phone Number: 617-724-2358 tel:617-724-2358
  Fax: 617-726-7422 tel:617-726-7422
 
  Bugs: surfer.nmr.mgh.harvard.edu/fswiki/BugReporting
  http://surfer.nmr.mgh.harvard.edu/fswiki/BugReporting
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  www.nmr.mgh.harvard.edu/facility/filedrop/index.html
  http://www.nmr.mgh.harvard.edu/facility/filedrop/index.html
  Outgoing:
  ftp://surfer.nmr.mgh.harvard.edu/transfer/outgoing/flat/greve/
 
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 gr...@nmr.mgh.harvard.edu
 Phone Number: 617-724-2358
 Fax: 617-726-7422

 Bugs: surfer.nmr.mgh.harvard.edu/fswiki/BugReporting
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xhemireg.rh.log
Description: Binary data


xhemireg.lh.log
Description: Binary data
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Re: [Freesurfer] Xhemi: vertex-wise volume asymmetry analysis?

2014-04-28 Thread Yizhou Ma
Hi Doug,

I just read from the .log files that the lh.thickness/area/pial/... files
in subject/xhemi/surf are copied from subject/surf at the end of the
command. Thus it seems that I can just copy lh.volume, too?

Thanks,
Cherry


On Mon, Apr 28, 2014 at 6:25 PM, Yizhou Ma ym...@nyu.edu wrote:

 Thanks Doug. I don't see an error message in xhemireg.?h.log files. Please
 see attached for the files.


 On Mon, Apr 28, 2014 at 4:22 PM, Douglas N Greve 
 gr...@nmr.mgh.harvard.edu wrote:


 Then something went wrong along the way. Check the xhemi/xhemireg.?h.log
 to see if there is an error. If not, send the log file to me

 On 04/28/2014 04:14 PM, Yizhou Ma wrote:
  Thanks Doug. I have ?h.volume in subject/surf but not in
  subject/xhemi/surf. I followed this page
  http://surfer.nmr.mgh.harvard.edu/fswiki/Xhemi and I think it is a
  problem with surfreg rather than mris_preproc. Can I just copy the
  ?h.volume from subject/surf to subject/xhemi/surf?
 
  Best,
  Cherry
 
 
  On Mon, Apr 28, 2014 at 4:01 PM, Douglas N Greve
  gr...@nmr.mgh.harvard.edu mailto:gr...@nmr.mgh.harvard.edu wrote:
 
 
 
  There should be a ?h.volume file. Are you sure there is not? You can
  specify -meas volume when you run mris_preproc
  doug
 
  On 04/27/2014 08:35 PM, Yizhou Ma wrote:
   Dear freesurfer experts,
  
   I am working on a project on cortical asymmetry and I have
 analyzed
   vertex-wise cortical thickness/surface area asymmetry. However, I
   noticed that Freesurfer doesn't not seem to facilitate such
 analysis
   for gray matter volume, as after running surfreg -xhemi there is
 no
   ?h.volume file in subject/xhemi/surf. I searched the literature
 and
   found studies on gray matter density asymmetry with VBM and
  ROI-based
   volume asymmetry. Is it true that Freesurfer doesn't offer
  vertex-wise
   volume asymmetry analysis? And may I know the rationale?
  
   Thanks,
   Cherry
  
  
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  mailto:Freesurfer@nmr.mgh.harvard.edu
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  --
  Douglas N. Greve, Ph.D.
  MGH-NMR Center
  gr...@nmr.mgh.harvard.edu mailto:gr...@nmr.mgh.harvard.edu
  Phone Number: 617-724-2358 tel:617-724-2358
  Fax: 617-726-7422 tel:617-726-7422
 
  Bugs: surfer.nmr.mgh.harvard.edu/fswiki/BugReporting
  http://surfer.nmr.mgh.harvard.edu/fswiki/BugReporting
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  www.nmr.mgh.harvard.edu/facility/filedrop/index.html
  http://www.nmr.mgh.harvard.edu/facility/filedrop/index.html
  Outgoing:
  ftp://surfer.nmr.mgh.harvard.edu/transfer/outgoing/flat/greve/
 
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 MGH-NMR Center
 gr...@nmr.mgh.harvard.edu
 Phone Number: 617-724-2358
 Fax: 617-726-7422

 Bugs: surfer.nmr.mgh.harvard.edu/fswiki/BugReporting
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[Freesurfer] Xhemi: vertex-wise volume asymmetry analysis?

2014-04-27 Thread Yizhou Ma
Dear freesurfer experts,

I am working on a project on cortical asymmetry and I have analyzed
vertex-wise cortical thickness/surface area asymmetry. However, I noticed
that Freesurfer doesn't not seem to facilitate such analysis for gray
matter volume, as after running surfreg -xhemi there is no ?h.volume file
in subject/xhemi/surf. I searched the literature and found studies on gray
matter density asymmetry with VBM and ROI-based volume asymmetry. Is it
true that Freesurfer doesn't offer vertex-wise volume asymmetry analysis?
And may I know the rationale?

Thanks,
Cherry
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Re: [Freesurfer] recon-all subject -qcache in a different version of freesurfer?

2014-04-25 Thread Yizhou Ma
Hi Doug,

To follow-up: I have been running recon-all subject -qcache but for some of
my subjects it exited with ERRORS due to unmatched number of vertices. I
read from previous threads that the general fix is to run -autorecon2 and
-autorecon3 again. However, I hesitate to do that in my case due to the
difference in Freesurfer versions. What do you think I can do?

Thanks,
Cherry


On Thu, Apr 24, 2014 at 12:06 PM, Yizhou Ma ym...@nyu.edu wrote:

 Great. Thanks Doug.


 On Thu, Apr 24, 2014 at 10:39 AM, Douglas N Greve 
 gr...@nmr.mgh.harvard.edu wrote:


 It is ok to run -qcache using 5.3.


 On 04/23/2014 10:54 PM, Yizhou Ma wrote:
  Dear Freesurfer experts,
 
  I have processed my images and done analysis with cortical thickness
  using FS510. Now that I want to do vertex-wise analysis with surface
  area and volume, I learned that there is a bug in FS510 with this.
  (
 https://mail.nmr.mgh.harvard.edu/pipermail//freesurfer/2011-August/019903.html
 )
  Before I go on to use recon-all subject -qcache in FS530 to generate
  vertex-wise surface area and volume maps, I want to make sure that
  this would not cause concerns about use of mixed versions of
  Freesurfer, or concerns about interpreting my cortical thickness and
  surface area/volume findings together. And if it does, what should I do?
 
  Thanks a lot,
  Cherry
 
 
 
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 Douglas N. Greve, Ph.D.
 MGH-NMR Center
 gr...@nmr.mgh.harvard.edu
 Phone Number: 617-724-2358
 Fax: 617-726-7422

 Bugs: surfer.nmr.mgh.harvard.edu/fswiki/BugReporting
 FileDrop: https://gate.nmr.mgh.harvard.edu/filedrop2
 www.nmr.mgh.harvard.edu/facility/filedrop/index.html
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Re: [Freesurfer] recon-all subject -qcache in a different version of freesurfer?

2014-04-24 Thread Yizhou Ma
Great. Thanks Doug.


On Thu, Apr 24, 2014 at 10:39 AM, Douglas N Greve gr...@nmr.mgh.harvard.edu
 wrote:


 It is ok to run -qcache using 5.3.


 On 04/23/2014 10:54 PM, Yizhou Ma wrote:
  Dear Freesurfer experts,
 
  I have processed my images and done analysis with cortical thickness
  using FS510. Now that I want to do vertex-wise analysis with surface
  area and volume, I learned that there is a bug in FS510 with this.
  (
 https://mail.nmr.mgh.harvard.edu/pipermail//freesurfer/2011-August/019903.html
 )
  Before I go on to use recon-all subject -qcache in FS530 to generate
  vertex-wise surface area and volume maps, I want to make sure that
  this would not cause concerns about use of mixed versions of
  Freesurfer, or concerns about interpreting my cortical thickness and
  surface area/volume findings together. And if it does, what should I do?
 
  Thanks a lot,
  Cherry
 
 
 
  ___
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  Freesurfer@nmr.mgh.harvard.edu
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 --
 Douglas N. Greve, Ph.D.
 MGH-NMR Center
 gr...@nmr.mgh.harvard.edu
 Phone Number: 617-724-2358
 Fax: 617-726-7422

 Bugs: surfer.nmr.mgh.harvard.edu/fswiki/BugReporting
 FileDrop: https://gate.nmr.mgh.harvard.edu/filedrop2
 www.nmr.mgh.harvard.edu/facility/filedrop/index.html
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[Freesurfer] recon-all subject -qcache in a different version of freesurfer?

2014-04-23 Thread Yizhou Ma
Dear Freesurfer experts,

I have processed my images and done analysis with cortical thickness using
FS510. Now that I want to do vertex-wise analysis with surface area and
volume, I learned that there is a bug in FS510 with this. (
https://mail.nmr.mgh.harvard.edu/pipermail//freesurfer/2011-August/019903.html
)
Before I go on to use recon-all subject -qcache in FS530 to generate
vertex-wise surface area and volume maps, I want to make sure that this
would not cause concerns about use of mixed versions of Freesurfer, or
concerns about interpreting my cortical thickness and surface area/volume
findings together. And if it does, what should I do?

Thanks a lot,
Cherry
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[Freesurfer] Total gray matter volume for aparc.2009?

2013-09-16 Thread Yizhou Ma
Dear Freesurfer experts,

I'm doing ROI analysis on surface area and gray matter volume using the
aparc.2009 parcellation. Now that I wanted to use corresponding whole brain
measures as covariates, I could not find whole brain gray matter volume
measures in the aparc.a2009s.stats file. However, I find one in the
aseg.stats file. Since I believe these two file use different approaches to
estimate gray matter volume, I'm stuck with what I choose for the whole
brain measure. Do I use the value in aseg.stats or do I add up the volumes
of regions in aparc.a2009s.stats? And advice is appreciated.

Thanks,
Cherry
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Re: [Freesurfer] Total gray matter volume for aparc.2009?

2013-09-16 Thread Yizhou Ma
This is very helpful, Doug. Then I should the value in aseg.stats. Thank
you very much.


On Mon, Sep 16, 2013 at 4:20 PM, Douglas Greve gr...@nmr.mgh.harvard.eduwrote:


 Hi Cherry, the total gray matter volume in aseg.stats is a combination of
 the two approaches. For cortical structures, it computes the volume using
 surface-based methods. The subcortical structures it uses the volume-based
 method. To compute total GM, you need both.
 doug



 On 9/16/13 9:11 PM, Yizhou Ma wrote:

 Dear Freesurfer experts,

  I'm doing ROI analysis on surface area and gray matter volume using the
 aparc.2009 parcellation. Now that I wanted to use corresponding whole brain
 measures as covariates, I could not find whole brain gray matter volume
 measures in the aparc.a2009s.stats file. However, I find one in the
 aseg.stats file. Since I believe these two file use different approaches to
 estimate gray matter volume, I'm stuck with what I choose for the whole
 brain measure. Do I use the value in aseg.stats or do I add up the volumes
 of regions in aparc.a2009s.stats? And advice is appreciated.

  Thanks,
 Cherry


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Re: [Freesurfer] temporal lobe left out in pial surface - edits on brainmask.mgz or brain.finalsurfs.mgz?

2013-05-30 Thread Yizhou Ma
Hi all,

This is a follow-up on my question so that people can know what I've tried
and how it works. I added control points in the white matter and the
delineation of the temporal lobe has improved to a satisfying level. We
also find what Michael has suggested very interesting. But it seems that if
we change the parameters for certain subjects, we would have to rerun all
of the other subjects with the same set of parameters, which may not be
desirable at the moment.

Thanks for all the help,
Cherry


On Wed, May 22, 2013 at 4:46 PM, Yizhou Ma ym...@nyu.edu wrote:

 Hi Michael,

 This method seems cool. I'll try to implement it. Thank you so much.

 Best,
 Cherry


 On Wed, May 22, 2013 at 4:17 PM, Harms, Michael mha...@wustl.edu wrote:


  I've also posted in the past on a set of expert options that helped
 our surfaces in the temporal lobe considerably.  You should be able to
 locate that post in the archives.

   --
 Michael Harms, Ph.D.
  ---
 Conte Center for the Neuroscience of Mental Disorders
 Washington University School of Medicine
 Department of Psychiatry, Box 8134
 660 South Euclid Ave. Tel: 314-747-6173
 St. Louis, MO  63110 Email: mha...@wustl.edu

   From: Yizhou Ma ym...@nyu.edu
 Date: Wednesday, May 22, 2013 2:30 PM
 To: Bruce Fischl fis...@nmr.mgh.harvard.edu
 Cc: freesurfer freesurfer@nmr.mgh.harvard.edu, Chris Watson 
 christopher.wat...@childrens.harvard.edu

 Subject: Re: [Freesurfer] temporal lobe left out in pial surface - edits
 on brainmask.mgz or brain.finalsurfs.mgz?

   Ok. I'll give it a try. Thanks, Chris  Bruce!


 On Wed, May 22, 2013 at 3:29 PM, Bruce Fischl fis...@nmr.mgh.harvard.edu
  wrote:

 Hi Cherry

 Chris is right. Putting control points in the WM will help the pial
 surface as well.

 cheers
 Bruce

 On Wed, 22 May 2013, Yizhou Ma wrote:

  Hi Chris,
 Thanks a lot. I agree that control points should actually only be put in
 white matter. I'm just not sure if I can expand the pial surface to a
 satisfactory extend if I only do edits in the white matter. It seems to
 me
 that the problem here is that the signal weakened in the lower part of
 the
 brain so that the gray matter doesn't have adequate intensity to be
 included
 in the pial surface (the values are around 30).

 Thanks,
 Cherry


 On Wed, May 22, 2013 at 3:14 PM, Chris Watson
 Christopher.Watson@childrens.**harvard.educhristopher.wat...@childrens.harvard.edu
 wrote:
   You need to place the control points in the white matter, not
   around it.

   On 05/22/2013 03:04 PM, Yizhou Ma wrote:
   Dear FS experts,
 I have a subject where large portions of temporal lobe is not
 included in the pial surface. A member in my lab has suggested
 edits on brain.finalsurfs.mgz. However, in the following page it
 is indicated that edits on brain.finalsurfs.mgz is only intended
 for cases regarding inclusion of cerebellum. Can anyone please
 let me know which volume I should be working on in this case?

 http://ftp.nmr.mgh.harvard.**edu/fswiki/FsTutorial/**PialEditshttp://ftp.nmr.mgh.harvard.edu/fswiki/FsTutorial/PialEdits

 Thanks,
 Cherry


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 are not the intended recipient, be advised that any unauthorized use,
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Re: [Freesurfer] temporal lobe left out in pial surface - edits on brainmask.mgz or brain.finalsurfs.mgz?

2013-05-22 Thread Yizhou Ma
Hi Chris,

Thanks a lot. I agree that control points should actually only be put in
white matter. I'm just not sure if I can expand the pial surface to a
satisfactory extend if I only do edits in the white matter. It seems to me
that the problem here is that the signal weakened in the lower part of the
brain so that the gray matter doesn't have adequate intensity to be
included in the pial surface (the values are around 30).

Thanks,
Cherry


On Wed, May 22, 2013 at 3:14 PM, Chris Watson 
christopher.wat...@childrens.harvard.edu wrote:

  You need to place the control points in the white matter, not around it.

  On 05/22/2013 03:04 PM, Yizhou Ma wrote:

 Dear FS experts,

  I have a subject where large portions of temporal lobe is not included
 in the pial surface. A member in my lab has suggested edits on
 brain.finalsurfs.mgz. However, in the following page it is indicated that
 edits on brain.finalsurfs.mgz is only intended for cases regarding
 inclusion of cerebellum. Can anyone please let me know which volume I
 should be working on in this case?

  http://ftp.nmr.mgh.harvard.edu/fswiki/FsTutorial/PialEdits

  Thanks,
  Cherry


 ___
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 contains patient information, please contact the Partners Compliance HelpLine 
 athttp://www.partners.org/complianceline . If the e-mail was sent to you in 
 error
 but does not contain patient information, please contact the sender and 
 properly
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Re: [Freesurfer] temporal lobe left out in pial surface - edits on brainmask.mgz or brain.finalsurfs.mgz?

2013-05-22 Thread Yizhou Ma
Ok. I'll give it a try. Thanks, Chris  Bruce!


On Wed, May 22, 2013 at 3:29 PM, Bruce Fischl fis...@nmr.mgh.harvard.eduwrote:

 Hi Cherry

 Chris is right. Putting control points in the WM will help the pial
 surface as well.

 cheers
 Bruce

 On Wed, 22 May 2013, Yizhou Ma wrote:

  Hi Chris,
 Thanks a lot. I agree that control points should actually only be put in
 white matter. I'm just not sure if I can expand the pial surface to a
 satisfactory extend if I only do edits in the white matter. It seems to me
 that the problem here is that the signal weakened in the lower part of the
 brain so that the gray matter doesn't have adequate intensity to be
 included
 in the pial surface (the values are around 30).

 Thanks,
 Cherry


 On Wed, May 22, 2013 at 3:14 PM, Chris Watson
 Christopher.Watson@childrens.**harvard.educhristopher.wat...@childrens.harvard.edu
 wrote:
   You need to place the control points in the white matter, not
   around it.

   On 05/22/2013 03:04 PM, Yizhou Ma wrote:
   Dear FS experts,
 I have a subject where large portions of temporal lobe is not
 included in the pial surface. A member in my lab has suggested
 edits on brain.finalsurfs.mgz. However, in the following page it
 is indicated that edits on brain.finalsurfs.mgz is only intended
 for cases regarding inclusion of cerebellum. Can anyone please
 let me know which volume I should be working on in this case?

 http://ftp.nmr.mgh.harvard.**edu/fswiki/FsTutorial/**PialEditshttp://ftp.nmr.mgh.harvard.edu/fswiki/FsTutorial/PialEdits

 Thanks,
 Cherry


 __**_
 Freesurfer mailing list
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 https://mail.nmr.mgh.harvard.**edu/mailman/listinfo/**freesurferhttps://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer


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 is
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 e-mai
 l
 contains patient information, please contact the Partners Compliance
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 e at
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Re: [Freesurfer] temporal lobe left out in pial surface - edits on brainmask.mgz or brain.finalsurfs.mgz?

2013-05-22 Thread Yizhou Ma
Hi Michael,

This method seems cool. I'll try to implement it. Thank you so much.

Best,
Cherry


On Wed, May 22, 2013 at 4:17 PM, Harms, Michael mha...@wustl.edu wrote:


  I've also posted in the past on a set of expert options that helped
 our surfaces in the temporal lobe considerably.  You should be able to
 locate that post in the archives.

   --
 Michael Harms, Ph.D.
  ---
 Conte Center for the Neuroscience of Mental Disorders
 Washington University School of Medicine
 Department of Psychiatry, Box 8134
 660 South Euclid Ave. Tel: 314-747-6173
 St. Louis, MO  63110 Email: mha...@wustl.edu

   From: Yizhou Ma ym...@nyu.edu
 Date: Wednesday, May 22, 2013 2:30 PM
 To: Bruce Fischl fis...@nmr.mgh.harvard.edu
 Cc: freesurfer freesurfer@nmr.mgh.harvard.edu, Chris Watson 
 christopher.wat...@childrens.harvard.edu

 Subject: Re: [Freesurfer] temporal lobe left out in pial surface - edits
 on brainmask.mgz or brain.finalsurfs.mgz?

   Ok. I'll give it a try. Thanks, Chris  Bruce!


 On Wed, May 22, 2013 at 3:29 PM, Bruce Fischl 
 fis...@nmr.mgh.harvard.eduwrote:

 Hi Cherry

 Chris is right. Putting control points in the WM will help the pial
 surface as well.

 cheers
 Bruce

 On Wed, 22 May 2013, Yizhou Ma wrote:

  Hi Chris,
 Thanks a lot. I agree that control points should actually only be put in
 white matter. I'm just not sure if I can expand the pial surface to a
 satisfactory extend if I only do edits in the white matter. It seems to
 me
 that the problem here is that the signal weakened in the lower part of
 the
 brain so that the gray matter doesn't have adequate intensity to be
 included
 in the pial surface (the values are around 30).

 Thanks,
 Cherry


 On Wed, May 22, 2013 at 3:14 PM, Chris Watson
 Christopher.Watson@childrens.**harvard.educhristopher.wat...@childrens.harvard.edu
 wrote:
   You need to place the control points in the white matter, not
   around it.

   On 05/22/2013 03:04 PM, Yizhou Ma wrote:
   Dear FS experts,
 I have a subject where large portions of temporal lobe is not
 included in the pial surface. A member in my lab has suggested
 edits on brain.finalsurfs.mgz. However, in the following page it
 is indicated that edits on brain.finalsurfs.mgz is only intended
 for cases regarding inclusion of cerebellum. Can anyone please
 let me know which volume I should be working on in this case?

 http://ftp.nmr.mgh.harvard.**edu/fswiki/FsTutorial/**PialEditshttp://ftp.nmr.mgh.harvard.edu/fswiki/FsTutorial/PialEdits

 Thanks,
 Cherry


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 e-mai
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 contains patient information, please contact the Partners Compliance
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 e at
 http://www.partners.org/**compliancelinehttp://www.partners.org/complianceline.
  If the e-mail was sent to you in er
 ror
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  --

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 Healthcare Information or other information of a sensitive nature. If you
 are not the intended recipient, be advised that any unauthorized use,
 disclosure, copying or the taking of any action in reliance on the contents
 of this information is strictly prohibited. If you have received this email
 in error, please immediately notify the sender via telephone or return mail.

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Re: [Freesurfer] mris_preproc with --xhemi

2013-04-02 Thread Yizhou Ma
 If I do use the LI maps to perform my stats, which would be the
 difference in interpretation between the results of using the
 --paired-diff maps, and the standarized LI maps?
The p-value will not change since the LI is just a scaled version of the
paired-diff
doug


In my opinion, the interpretation will be different, and the difference
just depends on the difference between (lh-rh) and LI themselves. The
former tells you if the lh is bigger than the rh in absolute values, while
the latter tells you to what extent it is asymmetrical. The latter is not a
scaled, but a standarized version of the former. Which you use in your
study really depends on which you are interested in conceptually.


On Tue, Apr 2, 2013 at 12:30 PM, Douglas N Greve
gr...@nmr.mgh.harvard.eduwrote:

 I think it is best to do it before the smoothing, though my results did
 not really change when I did it after.
 doug


 On 04/02/2013 12:07 PM, Gabriel Gonzalez Escamilla wrote:
  sorry I meant to do the division, to standarize the LI after the
  smoothing.
 
  El 02/04/13, *Gabriel Gonzalez Escamilla * ggon...@upo.es escribió:
  Dear Doug,
 
  Interesting but, if I'm understanding this well, to get a normal LI,
  the --paired-diff-norm should be divided by 2 not multiplied, isn't it?
 
  standardLI = (lh-rh)/(lh+rh)
  FS_LI = (lh-rh)/((lh+rh)/2) -- as you are dividing the denominator,
  your LI will be twice an standard one.
 
  Further, this division should be done before the smoothing, right?
 
  If I do use the LI maps to perform my stats, which would be the
  difference in interpretation between the results of using the
  --paired-diff maps, and the standarized LI maps?
 
  Regards,
  Gabriel.
 
 
  El 29/03/13, *Douglas N Greve * gr...@nmr.mgh.harvard.edu escribió:
  The --xhemi flag causes both the left and right hemispheres of each
  subject to be stacked into the output file. So the output file will
  have
  number of subjects x2 frames. The order is subject1.lh, subject1.rh,
  subject2.lh, subject2.rh, ...
 
  If you add the --paired-diff, then you will get number of frames =
  number of subjects, and each frame will be subject1.lh-rh,
  subject2.lh-rh, etc
 
  If you use the --paired-diff-norm instead, then you will get what you
  want subject1.(lh-rh)/((lh+rh)/2), subject2.(lh-rh)/((lh+rh)/2), etc.
 
  Note that most laterality indices (LI) are (lh-rh)/(lh+rh), so you
  would
  need to multiply the paired-diff-norm by 2.
 
  Finally, I want to point out that it may be better to smooth before
  computing the LI because the LI computation is non-linear and it has
  the
  potential to divide by a noisy number. To do this, run without the
  --paired-diff flags, then smooth, then run
 
  mri_concat yourfile.smoothed.mgh --paired-diff-norm --o
  yourfile.smoothed.LI.mgh
 
  In my study, I ran it both ways and it did not make a difference, but I
  think smoothing before LI is the safer bet.
 
  doug
 
 
  On 03/29/2013 12:33 PM, Ejoe Yizhou Ma wrote:
   Hi freesurfer experts,
  
   I'm investigating lh-to-rh asymmetry and am following instructions on
   this page :http://surfer.nmr.mgh.harvard.edu/fswiki/Xhemi.
   My question is, what does the --xhemi flag do in the mris_preproc
   command? It seems to me that it calculates (lh-rh) value at each
   vertex for every subject. (I tried to use the same subject for each
   pair of input for --paired-diff, and the result is not a all-zero
   output.)
  
   It would also be nice if someone can tell me how to realize my final
   goal, which is to get the (lh-rh)/[(lh+rh)/2] value at each vertex
  for
   each subject in a .mgh file.
  
   Thanks,
   Cherry
  
  
   ___
   Freesurfer mailing list
   Freesurfer@nmr.mgh.harvard.edu
   https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer
 
  --
  Douglas N. Greve, Ph.D.
  MGH-NMR Center
  gr...@nmr.mgh.harvard.edu
  Phone Number: 617-724-2358
  Fax: 617-726-7422
 
  Bugs: surfer.nmr.mgh.harvard.edu/fswiki/BugReporting
  FileDrop: www.nmr.mgh.harvard.edu/facility/filedrop/index.html
  Outgoing:
 ftp://surfer.nmr.mgh.harvard.edu/transfer/outgoing/flat/greve/
 
  ___
  Freesurfer mailing list
  Freesurfer@nmr.mgh.harvard.edu
  https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer
 
 
  The information in this e-mail is intended only for the person to
  whom it is
  addressed. If you believe this e-mail was sent to you in error and
  the e-mail
  contains patient information, please contact the Partners Compliance
  HelpLine at
  http://www.partners.org/complianceline . If the e-mail was sent to
  you in error
  but does not contain patient information, please contact the sender
  and properly
  dispose of the e-mail.
 
 
  --
  --
  PhD. student Gabriel González-Escamilla
  Laboratory of Functional Neuroscience
  Department of Physiology, Anatomy, and Cell Biology
  University Pablo de Olavide
  Ctra. de Utrera, Km.1

[Freesurfer] mri_glmfit-sim doesn't recognize --label flag

2013-03-30 Thread Yizhou Ma
Dear freesurfer experts,

I'm doing small volume correction for multiple comparison. However, when I
run mri_glmfit-sim with --label mylabelfile, I got the error that
--label unrecognizable. I use mri_glmfit-sim --help to see the help
information but there is no information regarding --label or --mask.
May I know why this?

P.S. I use FS510. I have run mri_glmfit-sim successfully without a mask.

Thanks,
Cherry
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[Freesurfer] mris_preproc with --xhemi

2013-03-29 Thread Ejoe Yizhou Ma
Hi freesurfer experts,

I'm investigating lh-to-rh asymmetry and am following instructions on this
page :http://surfer.nmr.mgh.harvard.edu/fswiki/Xhemi.
My question is, what does the --xhemi flag do in the mris_preproc
command? It seems to me that it calculates (lh-rh) value at each vertex for
every subject. (I tried to use the same subject for each pair of input for
--paired-diff, and the result is not a all-zero output.)

It would also be nice if someone can tell me how to realize my final goal,
which is to get the (lh-rh)/[(lh+rh)/2] value at each vertex for each
subject in a .mgh file.

Thanks,
Cherry
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Re: [Freesurfer] mris_preproc with --xhemi

2013-03-29 Thread Ejoe Yizhou Ma
Thanks, Doug. This is very helpful!


On Fri, Mar 29, 2013 at 12:48 PM, Douglas N Greve gr...@nmr.mgh.harvard.edu
 wrote:

 The --xhemi flag causes both the left and right hemispheres of each
 subject to be stacked into the output file. So the output file will have
 number of subjects x2 frames. The order is subject1.lh, subject1.rh,
 subject2.lh, subject2.rh, ...

 If you add the --paired-diff, then you will get number of frames =
 number of subjects, and each frame will be subject1.lh-rh,
 subject2.lh-rh, etc

 If you use the --paired-diff-norm instead, then you will get what you
 want subject1.(lh-rh)/((lh+rh)/2), subject2.(lh-rh)/((lh+rh)/2), etc.

 Note that most laterality indices (LI) are (lh-rh)/(lh+rh), so you would
 need to multiply the paired-diff-norm by 2.

 Finally, I want to point out that it may be better to smooth before
 computing the LI because the LI computation is non-linear and it has the
 potential to divide by a noisy number. To do this, run without the
 --paired-diff flags, then smooth, then run

 mri_concat yourfile.smoothed.mgh --paired-diff-norm --o
 yourfile.smoothed.LI.mgh

 In my study, I ran it both ways and it did not make a difference, but I
 think smoothing before LI is the safer bet.

 doug


 On 03/29/2013 12:33 PM, Ejoe Yizhou Ma wrote:
  Hi freesurfer experts,
 
  I'm investigating lh-to-rh asymmetry and am following instructions on
  this page :http://surfer.nmr.mgh.harvard.edu/fswiki/Xhemi.
  My question is, what does the --xhemi flag do in the mris_preproc
  command? It seems to me that it calculates (lh-rh) value at each
  vertex for every subject. (I tried to use the same subject for each
  pair of input for --paired-diff, and the result is not a all-zero
  output.)
 
  It would also be nice if someone can tell me how to realize my final
  goal, which is to get the (lh-rh)/[(lh+rh)/2] value at each vertex for
  each subject in a .mgh file.
 
  Thanks,
  Cherry
 
 
  ___
  Freesurfer mailing list
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  https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer

 --
 Douglas N. Greve, Ph.D.
 MGH-NMR Center
 gr...@nmr.mgh.harvard.edu
 Phone Number: 617-724-2358
 Fax: 617-726-7422

 Bugs: surfer.nmr.mgh.harvard.edu/fswiki/BugReporting
 FileDrop: www.nmr.mgh.harvard.edu/facility/filedrop/index.html
 Outgoing: ftp://surfer.nmr.mgh.harvard.edu/transfer/outgoing/flat/greve/

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 e-mail
 contains patient information, please contact the Partners Compliance
 HelpLine at
 http://www.partners.org/complianceline . If the e-mail was sent to you in
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[Freesurfer] mris_thickness qcache for xhemi surfaces

2013-03-28 Thread Ejoe Yizhou Ma
Dear Freesurfer experts,

I'm trying to investigate lh-to-rh asymmetry, so I register my subjects to
the subject of fsaverage_sym following the instructions on this page:
http://surfer.nmr.mgh.harvard.edu/fswiki/Xhemi. After all the surfreg
commands I've got ?h.thickness files in subjid/xhemi/surf.

In my lab we generally process ?h.thickness files with the next two
commands before statistically analysis:

mris_thickness -max 10 subjid ?h thickness_max10
recon-all -qcache -measure thickness_max10 -target fsaverage_510 -fwhm 10
-no-isrunning -s subjid -hemi ?h

To keep with this protocol, my questions are
1) how do I use mris_thickness properly so that I don't overwrite the
files registered to fsaverage_510?
2) in recon-all -qcache, will it do the trick by substituting
fsaverage_sym for fsaverage_510?

Thank you in advance,
Cherry
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Re: [Freesurfer] mris_thickness qcache for xhemi surfaces

2013-03-28 Thread Ejoe Yizhou Ma
Thanks, Doug.

I'm not planning to use qdec. It's just that I used to generate the
?h.thickness_max10.fwhm10.fsaverage_510.mgh files (which are what we name
the files after running the two commands) before I use mris_preproc and
mri_glmfit. I also generally skip the smooth step in freesurfer tutorial
since the files are smoothed in the first place.

Any suggestions?

Cherry

On Thu, Mar 28, 2013 at 12:53 PM, Douglas N Greve gr...@nmr.mgh.harvard.edu
 wrote:



 Actually, #2 will not work. I don't think you can use qdec with the
 xhemi stuff anyway. You should use the mris_preproc command followed by
 mri_glmfit.
 doug


 On 03/28/2013 12:51 PM, Douglas N Greve wrote:
  On 03/28/2013 12:16 PM, Ejoe Yizhou Ma wrote:
  Dear Freesurfer experts,
 
  I'm trying to investigate lh-to-rh asymmetry, so I register my
  subjects to the subject of fsaverage_sym following the instructions on
  this page: http://surfer.nmr.mgh.harvard.edu/fswiki/Xhemi. After all
  the surfreg commands I've got ?h.thickness files in subjid/xhemi/surf.
 
  In my lab we generally process ?h.thickness files with the next two
  commands before statistically analysis:
 
  mris_thickness -max 10 subjid ?h thickness_max10
  recon-all -qcache -measure thickness_max10 -target fsaverage_510
  -fwhm 10 -no-isrunning -s subjid -hemi ?h
 
  To keep with this protocol, my questions are
  1) how do I use mris_thickness properly so that I don't overwrite
  the files registered to fsaverage_510?
  I think it will recreate the basic ?h.thickness file and will not touch
  registrations.
  2) in recon-all -qcache, will it do the trick by substituting
  fsaverage_sym for fsaverage_510?
 
  Yes, that should work.
  Thank you in advance,
  Cherry
 
 
 
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Re: [Freesurfer] mris_thickness qcache for xhemi surfaces

2013-03-28 Thread Ejoe Yizhou Ma
I see. Then I think I'd better just follow the instructions on the Xhemi
page. Thanks!

On Thu, Mar 28, 2013 at 1:06 PM, Douglas N Greve
gr...@nmr.mgh.harvard.eduwrote:

 Hi Cherry, what I meant was that you cannot use the qcache with xhemi.
 You'll need to run the mris_preproc command as listed on the web page.
 doug


 On 03/28/2013 01:00 PM, Ejoe Yizhou Ma wrote:

 Thanks, Doug.

 I'm not planning to use qdec. It's just that I used to generate the
 ?h.thickness_max10.fwhm10.**fsaverage_510.mgh files (which are what we
 name the files after running the two commands) before I use mris_preproc
 and mri_glmfit. I also generally skip the smooth step in freesurfer
 tutorial since the files are smoothed in the first place.

 Any suggestions?

 Cherry

 On Thu, Mar 28, 2013 at 12:53 PM, Douglas N Greve 
 gr...@nmr.mgh.harvard.edu 
 mailto:gr...@nmr.mgh.harvard.**edugr...@nmr.mgh.harvard.edu
 wrote:



 Actually, #2 will not work. I don't think you can use qdec with the
 xhemi stuff anyway. You should use the mris_preproc command
 followed by
 mri_glmfit.
 doug


 On 03/28/2013 12:51 PM, Douglas N Greve wrote:
  On 03/28/2013 12:16 PM, Ejoe Yizhou Ma wrote:
  Dear Freesurfer experts,
 
  I'm trying to investigate lh-to-rh asymmetry, so I register my
  subjects to the subject of fsaverage_sym following the
 instructions on
  this page: 
 http://surfer.nmr.mgh.harvard.**edu/fswiki/Xhemihttp://surfer.nmr.mgh.harvard.edu/fswiki/Xhemi
 .
 After all
  the surfreg commands I've got ?h.thickness files in
 subjid/xhemi/surf.
 
  In my lab we generally process ?h.thickness files with the next two
  commands before statistically analysis:
 
  mris_thickness -max 10 subjid ?h thickness_max10
  recon-all -qcache -measure thickness_max10 -target fsaverage_510
  -fwhm 10 -no-isrunning -s subjid -hemi ?h
 
  To keep with this protocol, my questions are
  1) how do I use mris_thickness properly so that I don't overwrite
  the files registered to fsaverage_510?
  I think it will recreate the basic ?h.thickness file and will
 not touch
  registrations.
  2) in recon-all -qcache, will it do the trick by substituting
  fsaverage_sym for fsaverage_510?
 
  Yes, that should work.
  Thank you in advance,
  Cherry
 
 
 
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 gr...@nmr.mgh.harvard.edu 
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 Phone Number: 617-724-2358 tel:617-724-2358
 Fax: 617-726-7422 tel:617-726-7422

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Re: [Freesurfer] contrast matrix

2012-11-18 Thread Yizhou Ma
Hi Doug,

Thanks for replying. Yet I have a question on this matrix. From what I see,
each row corresponds to one contrast, which has one df. Thus why are we
having 8 rows while the interaction has a df of 4?

Thanks,
Cherry

On Sun, Nov 18, 2012 at 6:23 PM, Douglas Greve gr...@nmr.mgh.harvard.eduwrote:


 Hi Bo, you can think of  the Ftest as a logical 'OR' between the t-test
 contrasts indicated in each row. Each row is a difference of differences, so

 1. (A-B)om - (A-B)pm -- Does the difference between A and B differ
 between groups O and P for Males?
 2. (A-B)om - (A-B)qm
 3. (A-C)om - (A-C)pm
 4. (A-C)om - (A-C)qm
 5. (A-B)of - (A-B)pf -- Does the difference between A and B differ
 between groups O and P for Females?
 6. (A-B)of - (A-B)qf
 7. (A-C)of - (A-C)pf
 8. (A-C)of - (A-C)pf

 I've put together the first 9 columns of the first 4 rows. The last 9
 columns are all 0s. For the last for rows, the 0s and below matrix are
 swapped to give you the same for the females

 doug

  AOM  BOM  APM  BPM  AQM  BQM  COM  CPM
 CQM
 --
  +AOM -BOM -APM -BPM0000
 0
  +AOM -BOM00 -AQM +BQM00
 0
  +AOM0 -APM000 -COM +CPM
 0
  +AOM000 -AQM  -COM0
 +CQM



 On 11/17/12 9:21 PM, xiangbo_2010 wrote:

   Hi Freesurfer experts,

  I'm very sorry to bother you, but I am very confused with the following
 questions:

 My experimental design includes three discrete factors:  factor
 1 with three levels (A,B,C ); factor 2 with three levels (O,P,Q); gender
 (F, M), and one covariate.

 So I can get 18 classes: FAO, FAP,FAQ,FBO,FBP,FBQ,FCO,FCP,FCQ,MAO,
 MAP,MAQ,MBO,MBP,MBQ,MCO,MCP,MCQ.  I want to perform the interaction
 between factor 1 and factor 2 regressing out the effect of gender and one
 covariate, but I don't know the rules for setting the contrasts for the
 F-test.  The contrast matrix I used is:

 1 1 1 -1 -1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

 1 -1 0 1 -1 0 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

 1 1 1 0 0 0 -1 -1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

 1 0 -1 1 0 -1 1 0 -1  0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
 0

 0 0 0 0 0 0 0 0 0 1 1 1 -1 -1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
 0

 0 0 0 0 0 0 0 0 0 1 -1 0 1 -1 0 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
 0

 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 -1 -1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
 0

 0 0 0 0 0 0 0 0 0 1 0 -1 1 0 -1 1 0 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
 0

 is it correct?



 Any help will be very appreciated.



 Best wishes,



 Bo Xiang










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Re: [Freesurfer] contrast matrix

2012-11-17 Thread Yizhou Ma
Hi Bo,

I'm definitely not an expert of this, but I see that you really nead some
help, so I tried to look at your matrix. From my humble opinion it doesn't
look correct. Firstly, the first 9 groups are male groups and the last 9
are female groups. Then why are all female groups assigned 0 in the matrix?
Secondly, since the interaction you are interested in have a df of 4, do
you think maybe you should have 4 rows rather than 8 in your matrix?

Cherry

On Sat, Nov 17, 2012 at 9:21 PM, xiangbo_2010 xiangbo_2...@126.com wrote:

  Hi Freesurfer experts,

  I'm very sorry to bother you, but I am very confused with the following
 questions:

 My experimental design includes three discrete factors:  factor
 1 with three levels (A,B,C ); factor 2 with three levels (O,P,Q); gender
 (F, M), and one covariate.

 So I can get 18 classes: FAO, FAP,FAQ,FBO,FBP,FBQ,FCO,FCP,FCQ,MAO,
 MAP,MAQ,MBO,MBP,MBQ,MCO,MCP,MCQ.  I want to perform the interaction
 between factor 1 and factor 2 regressing out the effect of gender and one
 covariate, but I don't know the rules for setting the contrasts for the
 F-test.  The contrast matrix I used is:

 1 1 1 -1 -1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

 1 -1 0 1 -1 0 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

 1 1 1 0 0 0 -1 -1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

 1 0 -1 1 0 -1 1 0 -1  0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
 0

 0 0 0 0 0 0 0 0 0 1 1 1 -1 -1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
 0

 0 0 0 0 0 0 0 0 0 1 -1 0 1 -1 0 1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
 0

 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 -1 -1 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
 0

 0 0 0 0 0 0 0 0 0 1 0 -1 1 0 -1 1 0 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
 0

 is it correct?



 Any help will be very appreciated.



 Best wishes,



 Bo Xiang









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Re: [Freesurfer] Paired t-test with independent samples

2012-07-24 Thread Yizhou Ma
Hi Jurgen,

I actually just came across a similar situation in which I tried to
convince my PI that a paired t test is more appropriate. In my humble
opinion, if you are applying an independent group test, than the matching
design is irrelevant in this analysis because you're not taking any
advantage that might accompany the matching. On the other hand, a paired t
test lends more power if your subjects are sufficiently matched, while
being more conservative when the matching is a failure (i.e. if you thick
the two groups are actually idependent. In that case you'll get the same t
value but a smaller df in a paired t analysis).

That being said, I suggest you consult a statistical advisor for the
appropriate solution.

Cherry

On Tue, Jul 24, 2012 at 11:53 AM, Douglas N Greve gr...@nmr.mgh.harvard.edu
 wrote:

 Hi Jurgen, I don't think it is too unusual to do this kind of thing, ie,
 a paired test where pairing is based on gender, age, and education, but
 you know your data and field better than I do. It is not the case that
 one is more conservative than another (they are the same) assuming that
 the underlying assumptions are met. The underlying assumptions are more
 stringent for the paired than for the unpaired.
 doug

 On 07/24/2012 06:22 AM, Jürgen Hänggi wrote:
  Dear statistic experts
 
  We have done statistics between two independent groups using an
 independent
  t-test. One group consistent of 13 male patients and the other group
  consistent of 13 male control subjects who were matched to the patients
 with
  respect to age and education (in years).
 
  The reviewer proposed that it might be more appropriate to use a paired
  t-test.
  However, we can imaging a design where such an approach is adequate e.g.
  monozygotic twins reared in shared environments who are discordant for a
  particular trait.
 
  In our opinion, the matching of only age and education is not sufficient
 in
  order to apply a paired t-test.
  In addition, the paired t-test is more anti-conservative than the
  independent t-test and therefore advantages our hypothesis and this
 might be
  identified as a statistical trick by other reviewers.
 
  Any comment about whether or not the suggested paired t-test is
 appropriate
  in our study is highly appreciated.
 
  Thanks in advance
  Regards
  Jürgen Hänggi
 
 
 
  Jürgen Hänggi, Ph.D.
  Division Neuropsychology
  Institute of Psychology
  University of Zurich
  Binzmuehlestrasse 14, PO Box 25
  8050 Zurich, Switzerland
  0041 44 635 73 97 (phone office)
  0041 76 445 86 84 (phone mobile)
  0041 44 635 74 09 (fax office)
  BIN 4.D.04 (office room number)
  j.haenggi[at]psychologie.uzh.ch (email)
  http://www.psychologie.uzh.ch/neuropsy/ (website)
  http://www.juergenhaenggi.ch (private website)
 
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  e-mail in error) please notify the sender immediately and destroy this
  e-mail. Any unauthorised copying, disclosure or distribution of the
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