[Histonet] Re: Protecting fingers during microtomy
Me...while in training on a clean blade because the supervisor walked up behind me and startled me while I was putting a clean one on after cutting a chunk of bone... little cut and only an incident report. I was told when I entered that lab that you aren't a real histotech until you have cut yourself at least once? The students coming out of our histo school use surgipath's cut resistant gloves but they are only cut resistant... not completely cut proof because when EHS tried to force us all to use them one of my colleagues cut herself pretty good while using them Laurie Popp, BA HT (ASCP) MCR ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] infiltrating paraffin disposal
Hi All, Is it appropriate to dispose of paraffin used in processing by simply sending it out with the day's trash? Will the xylene contained in the solidified block leach out? Thanks, Fred ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cryostat SOP/fibrin and plasmin antibodies
Thanks again for all responses to my cryostat SOP question. I guess it's time now to write the dang thing. While I'm on here, can anyone recommend a vendor for fibrin and plasmin (plasminogen) antibodies that are reactive in mouse tissue and possibly share a working dilution for either of these? Thanks for any help! This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] bone marrow biopsies
We are currently using 10% formalin fixation on our bone marrow cores. We fix for 2 hours minimum prior to decal. We are using Immunocal from Decal Corp. for 2-4 hrs followed with processing overnight in VIP. Cores are still crunchy upon sectioning and we are doing surface decal for up to 30 min. Our paths want cores turned out within 24 hrs following procedure. Any suggestions? Thanks. Cindi ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC equipment
I am helping to set up an in office lab for a group of urologists. What is the best instrumentation for prostate tripple stain? What are the best reagents? Test volume will be relatively low. Perhaps as many as 5 additional immunostains will be added in the future. Testing will be done by an experienced histotech with minimal exposure to immunoperoxidase techniques. Goals are to maximize simplicity, dependability and value. Thanks in advance for any and all advice. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] progressive HE staining using Gills
We just switched our HE stain to a progressive stain using Gill II hematoxylin. Our derm guys love it, but now our GI docs are screaming bloody murder. Does anyone have suggestions for a protocol that will make both 'relatively happy'?? Thanks for letting me pick your brains again!!! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:[EMAIL PROTECTED] N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: bone marrow biopsies
Cindi Robinson asks: We are currently using 10% formalin fixation on our bone marrow cores. We fix for 2 hours minimum prior to decal. We are using Immunocal from Decal Corp. for 2-4 hrs followed with processing overnight in VIP. Cores are still crunchy upon sectioning and we are doing surface decal for up to 30 min. Our paths want cores turned out within 24 hrs following procedure. Any suggestions? An ordinary decalcifier (such as Decal Corp's Decal, or several others - just don't use nitric acid) should suffice, and will decalcify marrow cores in an hour or two. Bone marrow specimens obtained after 2 PM should be processed the following day. Would your pathologists and oncologists consent to this compromise? - This is a matter on which, in my experience, pathologists and oncologists don't communicate with each other very well. Bob Richmond Samurai Pathologist Knoxville TN In commUnIcate, the U comes before the I. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] progressive HE staining using Gills
Angela, Were you using Harris before? If so, the difference is that Gill formulations stain the mucin of goblet cells quite a dark blue and many GI docs don't like that look. They can get used to it though as ours did at Sacred Heart Medical Center. Hope this helps. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com www.mohslabstaffing.com Email: [EMAIL PROTECTED] DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Angela Bitting Sent: Thursday, October 30, 2008 11:30 AM To: histonet Subject: [Histonet] progressive HE staining using Gills We just switched our HE stain to a progressive stain using Gill II hematoxylin. Our derm guys love it, but now our GI docs are screaming bloody murder. Does anyone have suggestions for a protocol that will make both 'relatively happy'?? Thanks for letting me pick your brains again!!! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.175 / Virus Database: 270.8.5/1756 - Release Date: 10/30/2008 2:35 PM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] progressive HE staining using Gills
Richard Allen 7211 Hematoxylin doesn't stain mucin blue, and is a beautiful all around nuclear stain.I'm sure they'll send you a sample. Mickie Johnson [EMAIL PROTECTED] Sent by: [EMAIL PROTECTED] 10/30/2008 02:16 PM Please respond to [EMAIL PROTECTED] To 'Angela Bitting' [EMAIL PROTECTED], histonet@lists.utsouthwestern.edu cc Subject RE: [Histonet] progressive HE staining using Gills Angela, Were you using Harris before? If so, the difference is that Gill formulations stain the mucin of goblet cells quite a dark blue and many GI docs don't like that look. They can get used to it though as ours did at Sacred Heart Medical Center. Hope this helps. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com www.mohslabstaffing.com Email: [EMAIL PROTECTED] DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Angela Bitting Sent: Thursday, October 30, 2008 11:30 AM To: histonet Subject: [Histonet] progressive HE staining using Gills We just switched our HE stain to a progressive stain using Gill II hematoxylin. Our derm guys love it, but now our GI docs are screaming bloody murder. Does anyone have suggestions for a protocol that will make both 'relatively happy'?? Thanks for letting me pick your brains again!!! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.175 / Virus Database: 270.8.5/1756 - Release Date: 10/30/2008 2:35 PM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Modified SDH
Hi Histonet, I would like to hear from anyone who does the Modified SDH method. This is the method that uses Phenazine Methosulphate to suppress the normal mitochondria stains only mitochondria with DNA mutations. We had been doing the test for about 5 years with good results, then we made up new solution can't seem to get it working again. We have done all the routine lab solutions have come up blank. The problem is our slides are showing little to no staining, where before there was a little differentiation the abnormal cells stained fairly dark. If anyone has an image of a normal muscle an abnormal muscle stained with this method it would be very helpful. Thanks for any help, Sharon Allen Neuropathology Lab HSC - Winnipeg, MB CA [EMAIL PROTECTED] This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destiné à la personne ou aux personnes à qui il est adressé. Il peut contenir des informations privilégiées ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autorisée est strictement défendue. Si vous n'êtes pas le destinataire de ce message, veuillez en informer l'expéditeur immédiatement et lui remettre l'original. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] bone marrow biopsies
You are being placed in a difficult position that will only translate into physician unhappiness, so even though they are in a hurry (and usually for valid clinical reasons) the fact is that compromises will affect something else. I'm assuming you are a one shift operation. My lab is 24 hours, which allows us sufficient time for fixation and decalcification while enabling slides to be out the next morning. While Dr. Richmond is correct that there are faster decalcifiers, I'm guessing they want immunohistochemistry in at least some cases and the mineral acids will destroy your hopes of good immuno staining. We use 10% formic acid and still have to decalcify for about six hours in order to have blocks that section well. If you had an evening shift I'd suggest shortening your processing time. Shortening fixation time is counter productive to good morphology and good IHC. Lastly, you don't indicate if your bone marrows arrive at all times of the day. If you are trying to complete fixation and decalcification on specimens arriving in the afternoon, all to be on the processor at the end of your shift, you end up in the position you find yourself currently, with blocks poorly decalcified that section poorly. The only way to make this work to everyone's satisfaction is to have a longer work day, if it is possible to stagger work shifts. This also presumes that you have multiple tissue processors and can place your bone marrows on a shorter cycle, perhaps along with other biopsies. Others here may be able to advise you regarding using microwave technology for fixation and decalcification which may ultimately save the day for you. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Cynthia Robinson Sent: Thursday, October 30, 2008 11:44 AM To: histonet Subject: [Histonet] bone marrow biopsies We are currently using 10% formalin fixation on our bone marrow cores. We fix for 2 hours minimum prior to decal. We are using Immunocal from Decal Corp. for 2-4 hrs followed with processing overnight in VIP. Cores are still crunchy upon sectioning and we are doing surface decal for up to 30 min. Our paths want cores turned out within 24 hrs following procedure. Any suggestions? Thanks. Cindi ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
