[Histonet] Cryostats
Can anyone provide information on portable cryostats? We need one that will handle only 4-5 frozens per week. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Floaters
ANP.23350 Phase II N/A YES NO *Are flotation baths clean and well-maintained, and is there a procedure for preventing cross-contamination of paraffin sections in the bath?* * * NOTE: Of particular importance are periodic water changes or blotting of the water surface so that sections from one patient block are not inadvertently carried over to another case (so-called floaters or extraneous tissue). COMMENTARY: On Mon, Nov 24, 2008 at 4:44 AM, Edwards, R.E. [EMAIL PROTECTED] wrote: I tend to try and poke them down with a metal coat hanger!. -Original Message- From: [EMAIL PROTECTED] [mailto: [EMAIL PROTECTED] On Behalf Of [EMAIL PROTECTED] Sent: 21 November 2008 18:17 To: [EMAIL PROTECTED] Subject: [Histonet] Floaters Would anyone care to share their policy on floaters? Also, does anyone know if there is a CAP policy in floaters? Teresa Hallada BS, MT/CT (ASCP) Lead Cytotechnologist MidMichigan Health - Gratiot [EMAIL PROTECTED] 989.463.1101 ext 3423 Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The Unknown HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Floaters
Rope and a cinder block??!! Happy Monday! -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Histonet Alias Sent: Monday, November 24, 2008 6:09 AM To: Edwards, R.E. Cc: [EMAIL PROTECTED]; [EMAIL PROTECTED] Subject: Re: [Histonet] RE: Floaters ANP.23350 Phase II N/A YES NO *Are flotation baths clean and well-maintained, and is there a procedure for preventing cross-contamination of paraffin sections in the bath?* * * NOTE: Of particular importance are periodic water changes or blotting of the water surface so that sections from one patient block are not inadvertently carried over to another case (so-called floaters or extraneous tissue). COMMENTARY: On Mon, Nov 24, 2008 at 4:44 AM, Edwards, R.E. [EMAIL PROTECTED] wrote: I tend to try and poke them down with a metal coat hanger!. -Original Message- From: [EMAIL PROTECTED] [mailto: [EMAIL PROTECTED] On Behalf Of [EMAIL PROTECTED] Sent: 21 November 2008 18:17 To: [EMAIL PROTECTED] Subject: [Histonet] Floaters Would anyone care to share their policy on floaters? Also, does anyone know if there is a CAP policy in floaters? Teresa Hallada BS, MT/CT (ASCP) Lead Cytotechnologist MidMichigan Health - Gratiot [EMAIL PROTECTED] 989.463.1101 ext 3423 Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The Unknown HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RELIA Histology Careers Bulletin for 11-30 and A Few Holiday Shopping Tips!!
Hi Histonetters! I hope you are having a wonderful Fall. The weather is getting cooler and the Holidays are right around the corner. I realize that at this time of year a job change is the furthest thing from most peoples minds but I have some great opportunities that I wanted to share along with some great tips for holiday shopping. All of my positions are full time permanent positions with premier companies who offer excellent salaries, benefits and relocation assistance. Most of them are willing to look at onsite interviews and start dates after the holidays so if you or anyone you know might be looking now or after the first of the year it wouldnt hurt to shoot me a quick e-mail at mailto:[EMAIL PROTECTED] [EMAIL PROTECTED] or give me a quick call toll free at 866-607-3542. Currently I have management positions in CA, NH, OH and TX. I also have tech positions in CA, TX, WA, MD and MA. Holiday shopping is always fun, crazy, chaotic, inspiring, and challenging. Here are some tips that might save you some time, money and stress. Black Friday is the day after Thanksgiving the official first day of Holiday Shopping. If you want a heads up on the advertisements from your favorite stores go to the website: http://www.bfads.net/ www.bfads.net They have a lot of the ads posted on their site NOW that will be in your newspaper on Thanksgiving morning. The Monday after Thanksgiving is known as Cyber Monday it is the busiest online shopping day of the year and there is another website that will give you heads up on deals for Cyber Monday. This website is http://www.cybermonday.com/ www.cybermonday.com The best part of this site is that the stores that advertise on their site donate a percentage of sales to a scholarship fund. Again if you or anyone you know is interested in a new opportunity now or after the holidays get in touch with me. There are a lot of recruiters out there but remember I am the only one with the experience, connections and respect for you and your career that specializes exclusively in histology. I want to place you in a new position only if it is the right place, right time and right position for you. And I am always available for career advice, resume assistance or just a chat. Let me be your career advocate. Happy Holidays!!! Pam 866-607-3542 mailto:[EMAIL PROTECTED] [EMAIL PROTECTED] Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: mailto:[EMAIL PROTECTED] [EMAIL PROTECTED] mailto:[EMAIL PROTECTED] mailto:[EMAIL PROTECTED] http://home.earthlink.net/~relia1 http://home.earthlink.net/~relia1 http://www.myspace.com/pamatrelia www.myspace.com/pamatrelia http://www.myspace.com/pamatrelia http://www.myspace.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Career Opening: Histology Manager with leading lab in Dallas, TX
Currently a Histology Manager role is open with a leading, full-service clinical reference laboratory in Dallas, TX. Reporting to the Laboratory Director, accountabilities will include: - Management of all technical and operational activities of the Histology Department, including staffing, planning, coordination and evaluation - Troubleshooting quality and production issues including stains, processes, systems, and equipment. - Developing and implementing improvements in systems and processes for enhanced efficiency and quality, while managing growth. 8+ years of experience in histology with at least 5 years of supervisory experience is required for this position, with demonstrated management ability, organizational and training skills. Must have HT (ASCP) certification, minimum of Associates degree, excellent written and oral communication skills, as well as Microsoft Office and database management background. Our client is offering attractive base salary commensurate with experience, and relocation assistance is available. We are a part of a national search firm focused on the laboratory and biotechnology sectors. Our clients nationwide are premier laboratories with the latest in equipment and teams of valued professionals. Please contact Barbara Siegel at [EMAIL PROTECTED] if you are interested in an opportunity in this fine city. Career Studio national search Palm Beach, FL [EMAIL PROTECTED] 561-738-6363 ** One site has it all. Your email accounts, your social networks, and the things you love. Try the new AOL.com today!(http://pr.atwola.com/promoclk/10075x1212962939x1200825291/aol?redir=http://www.aol.com/?optin=new-dp %26icid=aolcom40vanity%26ncid=emlcntaolcom0001) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leica slide writer - problems??
Hi Joyce, Was this a recent problem, and has it been resolved? Thanks Steve -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Joyce Cline Sent: Thursday, November 20, 2008 6:20 PM To: Histonet Subject: RE: [Histonet] Leica slide writer - problems?? We have had the same problems, we discovered the slide surface on some of the snowcoat slides changed to a slick surface instead of a rough surface. We have switched to a pale gray slide that still has the rough surface and we have not had any printing wipe off. Joyce Cline, H.T. (ASCP) Technical Specialist Hagerstown Medical Lab. 301-665-4980 fax 301-665-4941 -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of [EMAIL PROTECTED] Sent: Thursday, November 20, 2008 7:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica slide writer - problems?? Hi, Has anyone else been having problems with there Lieca slide writer. Our problems mainly involve the ink writing rubbing off before and after staining!!, we use Surgipath snowcoated slides, what does everyone else use as they have hinted it could be our slides. Any help would be fantastic. Thanks Loads Sara ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * CONFIDENTIALITY NOTICE * This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Iron stains
We may be having a problem with our iron stains. I say that we 'may' be having a problem because our positive control always works. For bone marrows, we stain the core, aspirate, a smear, and a purchased positive control. The stain is all over the place. We may have a loaded smear with a negative core and aspirate. We may have a positive aspirate with a negative core and smear. We may have all negatives even though clinically, there should be iron. Our control always works. The pathologists do not trust the stain and really do not believe the results. I have stood by the stain due to the positive control but I find it increasingly difficult. I would appreciate any thoughts. Our procedures are outlined below... The aspirate is allowed to clot before placing it into 10%NBF. The core is placed briefly into DecalStat (until it floats) then placed into 10%NBF. Both are then processed with our regular tissues. The spend anywhere from 4 to 10 hours in formalin. The following day, they are cut and along with one of the purchased controls, run down to water on the stainer. (8 mins onboard oven, americlear, alcohols, water) The smear is placed into 95% alcohol then rinsed in distilled. All slides are then stained using Perl's Method for iron pigment. We have already explored the way the specimens are collected during the BM procedure, and decal solution. All reagents are good. I'm wondering about the formalin fixation. I know that threre are other probably better fixatives. Does anybody use another fixative prior to formalin? For those using formalin only, do you limit the fixation time in any way? I really would appreciate any comments. Such a common and simple stain. We should be able to trust it. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 [EMAIL PROTECTED] www.LMHealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Iron stains
I would always fix first before decal. The acid is stripping the iron. After discontinuing B5 fixative, we use B-Plus (zinc), but some are very happy with 10% NBF. The problem with purchased controls is that they are not treated the same as your material, but at least you know your reagents are good! My 2 cents... J:) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Tom McNemar Sent: Monday, November 24, 2008 12:07 PM To: [EMAIL PROTECTED] Subject: [Histonet] Iron stains We may be having a problem with our iron stains. I say that we 'may' be having a problem because our positive control always works. For bone marrows, we stain the core, aspirate, a smear, and a purchased positive control. The stain is all over the place. We may have a loaded smear with a negative core and aspirate. We may have a positive aspirate with a negative core and smear. We may have all negatives even though clinically, there should be iron. Our control always works. The pathologists do not trust the stain and really do not believe the results. I have stood by the stain due to the positive control but I find it increasingly difficult. I would appreciate any thoughts. Our procedures are outlined below... The aspirate is allowed to clot before placing it into 10%NBF. The core is placed briefly into DecalStat (until it floats) then placed into 10%NBF. Both are then processed with our regular tissues. The spend anywhere from 4 to 10 hours in formalin. The following day, they are cut and along with one of the purchased controls, run down to water on the stainer. (8 mins onboard oven, americlear, alcohols, water) The smear is placed into 95% alcohol then rinsed in distilled. All slides are then stained using Perl's Method for iron pigment. We have already explored the way the specimens are collected during the BM procedure, and decal solution. All reagents are good. I'm wondering about the formalin fixation. I know that threre are other probably better fixatives. Does anybody use another fixative prior to formalin? For those using formalin only, do you limit the fixation time in any way? I really would appreciate any comments. Such a common and simple stain. We should be able to trust it. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 [EMAIL PROTECTED] www.LMHealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Dissolve plastic
I Need Histonetters help. We have an eye with a plastic part for the lens, which is a type of telescope, I've been told. According to the new pathologist, we need to dissolve out this plastic lens, then he can gross the eye and we can process it into paraffin. We've never had to dissolve plastic before. The eye is in 10% NBF right now. I'm guess maybe something like chloroform? But what percent? For how long? Before or after grossing? Has anyone done this before, that can send a procedure to me? Thanks. *** Peggy A. Wenk, HTL(ASCP)SLS Program Director, Schools of Histotechnology Supervisor, Mortuary Services Laboratory Safety Officer, Disaster Preparedness and Quality Assurance Coordinator Department of Anatomic Pathology, 100RO William Beaumont Hospital 3601 W. 13 Mile Road Royal Oak, MI 48073-6769 Work 248/898-9079 Fax 248/898-9054 E-mail [EMAIL PROTECTED] Web Page: http://www.beaumont.edu/alliedhealth ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Peggy - eye/plastic lens
Hi Peggy: It is possible that acetone ( swab on, or soak) will dissolve or loosen the lens without cellular damage. I have not done this and will be interested to see what other suggestions are made. Vicki ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Floaters
Teresa Hallada in Michigan asks: Would anyone care to share their policy on floaters? Also, does anyone know if there is a CAP policy [on] floaters? At Gastonia we sent 'em on to the state ME's office - they had an adequately ventilated autopsy suite But seriously, folks, I've never heard of any regulatory agency having a policy on floaters - extraneous tissue on a slide that's floated in from some other case. What I was taught in residency - sometime in the late Ordovician period, I think it was - not to mention them in a report, but write floater on the slide before filing it. I've never heard anybody observe this, but there are two kinds of floaters: individual tissue sections (the responsibility of the microtomist) and whole chunks of tissue (the responsibility of whoever grossed the specimen). Since the trouble-shooting for these two problems is entirely different, you first have to determine which kind of floater you've got. Obviously if it's a whole chunk of tissue, you'll get repeated sections of it. If it's whole chunks of tissue, then the grosser isn't cleaning instruments adequately between cases. If I ran the zoo, I'd have several sets of instruments, throw them in a pot as I used them, and wash them when I ran out of instruments, but the Hospital Administrator's Official Handy-Dandy Book on How to Make Life Hard for the Anatomic Pathology Service specifies that there be only one set of grossing instruments in the lab at a time. (I've never actually seen this book, but I'm absolutely sure it exists.) Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] immuno set-up
I agree with Mr. Brooks on the Shandon Sequenza - fantastic for low volume. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJUSA Amos Brooks [EMAIL PROTECTED] 11/21/08 4:58 PM Hi, For small volumes like that, check with Thermo. They have various sizes of IHC stainers that have different slide capacities. Alternatively you could check out the Shandon Sequenza. I could find more info if you are interested. (BTW: I have no Thermo ties.) Amos Brooks Message: 19 Date: Thu, 20 Nov 2008 20:21:21 + From: [EMAIL PROTECTED] Subject: [Histonet] immuno set-up To: histonet@lists.utsouthwestern.edu Message-ID: [EMAIL PROTECTED] Content-Type: text/plain Hello all, I work at a private lab with one pathologist, and one histotech (me). We do about 5,000 cases a year. We are intrested in doing our immuno's in house (right now we send them out), and wanted to know your opinion on the type of equipment that would be best for this situation. Right now we send about 5 to 10 cases a week out for immuno's. All responses are very much appreciated Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Error question
Was wanting to know how people handle accession errors within the pathology information system,, to elaborate on this, is when a person receives in the specimen with a requisition and they have to do data entry into the system. What is allowable?, what is the percent error rate?, how are people dealing with errors? How are you dealing with personnel?? This is becoming an issue and I wanted to survey people out there how they are handling this issue,? Any help would greatly be appreciated. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 * Office: (928) 336-1743 *Fax: (928) 336-7319 *Email: [EMAIL PROTECTED] This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ?
I have a patient requesting her gall bladder be returned to her for religious reasons. The premise I've been given is so that, upon death, the patient may be stored with her body parts. My facility has concerns about providing it to her in formalin (for obvious reasons) or alcohol. The patient admits this is a family practice with momma's appendix already being stored in the attic. It can get a bit toasty warm here in the South so attic storage of a specimen in alcohol may not be prudent and I can't be absolutely certain it wouldn't burn the house down, another potential liability for my institution. I'm tempted to give it to her in food grade vinegar, to avoid the potential liabilities from using anything that could be considered hazardous. Assuming that returning her gall bladder is a given, what do you think of using vinegar for this purpose? Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ?
Could you give it to her in a plastic bag, with a container, and tell her to store it in rubbing alcohol? j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Della Speranza, Vinnie Sent: Monday, November 24, 2008 5:37 PM To: histonet Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? I have a patient requesting her gall bladder be returned to her for religious reasons. The premise I've been given is so that, upon death, the patient may be stored with her body parts. My facility has concerns about providing it to her in formalin (for obvious reasons) or alcohol. The patient admits this is a family practice with momma's appendix already being stored in the attic. It can get a bit toasty warm here in the South so attic storage of a specimen in alcohol may not be prudent and I can't be absolutely certain it wouldn't burn the house down, another potential liability for my institution. I'm tempted to give it to her in food grade vinegar, to avoid the potential liabilities from using anything that could be considered hazardous. Assuming that returning her gall bladder is a given, what do you think of using vinegar for this purpose? Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Dissolve plastic
Lee and Peggy- We section these eyes all the time with the plastic in place. I would certainly suggest a gluaraldehyde fixative. Straight NBF is a very poor fixative for the many tissues found in eyes, especially the retina. If you contact me directly, we can talk off line. Eyes have held a special fascination with me for many years. Joe Saby NAMSA From: Lee Peggy Wenk [EMAIL PROTECTED] To: histonet@lists.utsouthwestern.edu Sent: Monday, November 24, 2008 12:47:24 PM Subject: [Histonet] Dissolve plastic I Need Histonetters help. We have an eye with a plastic part for the lens, which is a type of telescope, I've been told. According to the new pathologist, we need to dissolve out this plastic lens, then he can gross the eye and we can process it into paraffin. We've never had to dissolve plastic before. The eye is in 10% NBF right now. I'm guess maybe something like chloroform? But what percent? For how long? Before or after grossing? Has anyone done this before, that can send a procedure to me? Thanks. *** Peggy A. Wenk, HTL(ASCP)SLS Program Director, Schools of Histotechnology Supervisor, Mortuary Services Laboratory Safety Officer, Disaster Preparedness and Quality Assurance Coordinator Department of Anatomic Pathology, 100RO William Beaumont Hospital 3601 W. 13 Mile Road Royal Oak, MI 48073-6769 Work 248/898-9079 Fax 248/898-9054 E-mail [EMAIL PROTECTED] Web Page: http://www.beaumont.edu/alliedhealth ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ?
You know, I really think that the vinegar is a great idea! I can't think of any reason off-hand why it would be hazardous, flammable, toxic, or bring on any liability. Even the isopropyl can be flammable, and if you told her to use it, you might be somewhat at fault ( though I know a bit of a stretch). In any case, I think that I'll try to remember that in case I encounter any similar requests... Thanks Joelle Date: Mon, 24 Nov 2008 17:40:38 -0500 From: [EMAIL PROTECTED] To: [EMAIL PROTECTED]; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? CC: Could you give it to her in a plastic bag, with a container, and tell her to store it in rubbing alcohol? j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax-Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Della Speranza, Vinnie Sent: Monday, November 24, 2008 5:37 PM To: histonet Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? I have a patient requesting her gall bladder be returned to her for religious reasons. The premise I've been given is so that, upon death, the patient may be stored with her body parts. My facility has concerns about providing it to her in formalin (for obvious reasons) or alcohol. The patient admits this is a family practice with momma's appendix already being stored in the attic. It can get a bit toasty warm here in the South so attic storage of a specimen in alcohol may not be prudent and I can't be absolutely certain it wouldn't burn the house down, another potential liability for my institution. I'm tempted to give it to her in food grade vinegar, to avoid the potential liabilities from using anything that could be considered hazardous. Assuming that returning her gall bladder is a given, what do you think of using vinegar for this purpose?Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Access your email online and on the go with Windows Live Hotmail. http://windowslive.com/Explore/Hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_access_112008___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Warthin-Starry // Best before dates
Hello! I use the Warthin-Starry technique to stain spirochaetes in FFPE sections. I don't know how long I can keep the AgNO_3 solutions and the hydrochinone solution whitout getting them to expire. Is there anyone who already had to deal with that question? Regards, Valentin ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
