RE: [Histonet] gram stain controls
We take two pieces of fresh tissue (umbilical cord works well) to micro and have them grow positive and negative on individual pieces. After a few days we fix the tissue and embed them together. Works great. PS we do the same when we need fungus controls. -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Rene J Buesa Sent: Tuesday, November 25, 2008 3:40 PM To: histonet@lists.utsouthwestern.edu; Fischer, R. B Cc: Delcambre, Linda V Subject: Re: [Histonet] gram stain controls I always used actual tissues with gram +/- bacteria because it is not the same (time wise) to stain a histogel than a piece of tissue. Appendix is a good control. René J. --- On Tue, 11/25/08, Fischer, R. B [EMAIL PROTECTED] wrote: From: Fischer, R. B [EMAIL PROTECTED] Subject: [Histonet] gram stain controls To: histonet@lists.utsouthwestern.edu Cc: Delcambre, Linda V [EMAIL PROTECTED] Date: Tuesday, November 25, 2008, 3:29 PM My pathologist has made a gram neg/pos control block for us to use. He made this by taking separate cultures of each and suspended them in histo gel. The control stains well. My question is: Since we are performing gram stain on tissues exclusively, will this cultured control be an accepted method as a control for tissues, or must we use tissue with gram pos/neg organisms. Thanks for your reply. R.Brian Fischer Histology Lead Tech Community Hospital of the Monterey Peninsula PO Box HH Monterey Ca. 93942 831-625-4791 Fax: 831-6583683 Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Epson v750 pro scanner
Dear Histoneters, Has anyone ever used the Epson v750 pro flatbed scanner to digitize microscopic slides? In the past we get used to a Nikon coolscan scanner and got nice results. We then bought the Epson scanner because of its high spatial resolution (6400 optical dpi) but did not obtain satisfactory images, principally because of bad focusing troubles. Does anyone has a good experience / expertise with this scanner and an efficient protocol to numerize slides with this device? Thanks to all, B. Delatour ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: As Thanksgiving Approaches
I am a Presbyterian and I have never heard of this. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 MarshallSlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Della Speranza, Vinnie Sent: Tuesday, November 25, 2008 5:10 PM To: 'Robert Richmond'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: As Thanksgiving Approaches Patient is listed in our system as Presbyterian. I'm guessing that patients know that if they cite religion as the basis for their request they are less likely to be denied. I like the mineral oil and glycerin suggestions as they are probably the least problematic from a safety perspective. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Robert Richmond Sent: Tuesday, November 25, 2008 4:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: As Thanksgiving Approaches I'd think that vésicule biliaire vinaigrette would be inclined to get the moldies. Of several not very satisfactory solutions, Tony Henwood's suggestion of mineral oil (paraffin oil) might be the safest, though messy if it gets spilled. What religion requires decades-long preservation of gallbladders? Highly observant Jews sometimes request return of tissues, but their requirement is that the tissue be buried in a Jewish cemetery - as soon as possible, not waiting for the rest of the patient to arrive. Do Muslims have any issues here? - I'm not aware of any Christian tradition that has any rules about this problem. In my personal experience, the most common problem of this sort has been the patient who wants an amputated leg buried with him. Whenever I've dealt with this problem, a funeral director has bailed me out. As far as I know, there was no religious issue with the legs, just personal (or cultural) preference. The most bizarre situation of this sort happened to me about ten years ago. A rural midwife had asked an OB-GYN to remove a retained placenta after a difficult delivery. The OB-GYN put the placenta in formalin and sent it to a pathology service some distance away. The midwife called the lab, and was furious to learn that the placenta had been put in formalin. It seems that (I hope you're not reading your e-mail with lunch) the midwife had her patients eat their babies' placentas. I think the JCAHO or somebody banned returning gallstones to patients, a practice that used to be quite a nuisance for pathologists. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Happy Thanksgiving from the Team at CompHealth
CompHealth would like to wish everyone a Happy and Safe Thanksgiving! Get in touch with us anytime and let us help you with your laboratory staffing needs. We concentrate on Permanent placement as well as Contract/Travel work. We would be happy to talk with you about what we can offer. Contact us by phone, email or visit our website at www.comphealth.com. If you are interested in learning more about contract/travel work get in touch with me. We are receiving positions almost daily and need qualified techs to fill them. In 1979, CompHealth was awarded a federal grant to organize short-term physician staffing services for needy areas throughout the western United States. These services were so successful that healthcare organizations across the country began requesting temporary physician coverage. The locum tenens industry was born! For the first ten years, CompHealth focused exclusively on temporary physician staffing services and became the largest locum tenens staffing firm in the U.S. From our leadership position in locum tenens, CompHealth broadened its business lines to become the leading national provider of allied health professionals, both temporary staffing and permanent placement. Today, CompHealth is the best single resource for healthcare professionals seeking employment and for healthcare organizations seeking complete recruiting and staffing services. Paul Kammeyer Staffing Consultant Lab Specialties - CompHealth P.O. Box 713400 Salt Lake City, UT 84171-3400 Phone: (800) 447-6016 ext. 3380 Fax: (866) 588-1340, (801) 930-4504 [EMAIL PROTECTED] www.comphealth.com Ask me about our $500 Referral Bonus Program!! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] Can you believe it
-Original Message- From: Woodward, Denise Sent: Wednesday, November 26, 2008 2:00 PM To: 'Webb, Dorothy L' Subject: RE: [Histonet] Can you believe it Yes I Have heard of total disintegration. A histologist friend in Maine told me about having to take the heat knob off a combination heat/stir plate when the heat knob was turned on instead of the stir knob and the cassettes of bone to be decalcified completely disintegrated by morning. This happened twice. The heat knob was removed before the third occurrence could transpire! Denise Long Woodward -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Webb, Dorothy L Sent: Tuesday, November 25, 2008 4:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Can you believe it Has anyone ever heard of cassettes and tissue totally disintegrating in formic acid decal solution with a high heat on the solution for about 3 hours? We had 3 cassettes with tissue for decal placed in our container on the platform for adding agitation. Someone had moved the knob to heat and this wasn't noticed until about 2-3 hours later, when it was boiling! We cannot locate the cassettes (I think someone misplaced them) and others think they totally disintegrated with the decal and heat...what does everyone think? In all of my years of histology, never have I heard of anything so weird .. Dorothy Webb This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Can you believe it
Yes, this happened in our lab also. It was overnight and not 3 hours but it was all gone in the AM. We weren't even allowed to keep the stir plate after that one. Sheila Adey HT MLT Port Huron Hospital Michigan Date: Wed, 26 Nov 2008 14:03:23 -0500 From: [EMAIL PROTECTED] To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Can you believe it-Original Message- From: Woodward, Denise Sent: Wednesday, November 26, 2008 2:00 PM To: 'Webb, Dorothy L' Subject: RE: [Histonet] Can you believe it Yes I Have heard of total disintegration. A histologist friend in Maine told me about having to take the heat knob off a combination heat/stir plate when the heat knob was turned on instead of the stir knob and the cassettes of bone to be decalcified completely disintegrated by morning. This happened twice. The heat knob was removed before the third occurrence could transpire! Denise Long Woodward -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Webb, Dorothy L Sent: Tuesday, November 25, 2008 4:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Can you believe it Has anyone ever heard of cassettes and tissue totally disintegrating in formic acid decal solution with a high heat on the solution for about 3 hours? We had 3 cassettes with tissue for decal placed in our container on the platform for adding agitation. Someone had moved the knob to heat and this wasn't noticed until about 2-3 hours later, when it was boiling! We cannot locate the cassettes (I think someone misplaced them) and others think they totally disintegrated with the decal and heat...what does everyone think? In all of my years of histology, never have I heard of anything so weird .. Dorothy Webb This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
