Re: [Histonet] problem with NSH website???
I am not sure what is going on Chris. I just tried it and I got the same result you did going to Isovers etc. Obviously something is very wrong and they need to correct it. I will forward you e-mail and my answer to them so they know it is not just a problem for Europe. I assume they monitor HistoNet - I could be wrong. Pam Marcum - Original Message - From: C.M. van der Loos [EMAIL PROTECTED] To: histonet netserver histonet@lists.utsouthwestern.edu Sent: Friday, November 28, 2008 4:02:21 AM GMT -05:00 US/Canada Eastern Subject: [Histonet] problem with NSH website??? Dear All,Desperately I tried to submit my abstracts for the NSH convention. Using the links for submission in the email we got from Aubrey Wanner/Kim Simmons early November, the browser goes to Isovera.com a domain for sale in Germany, provided by Sedo Domain Parking. Obviously something that has nothing to do with NSH. The same problems occurs at the NSH website. You can go to the NSH website as normal, but clicking on 'meetings/event' and selecting 'symposium/convention' one is re-directed to that same crap. What is going on here??? Does this problem also occurs with others???Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sakura tape problem
Be careful! The Acetone dissolves the tape! Janet L. Bonner, HTL (ASCP) Pathology Laboratory Florida Hospital Winter Park [EMAIL PROTECTED] mailto:[EMAIL PROTECTED] 407-646-7559 From: [EMAIL PROTECTED] on behalf of Weems, Joyce Sent: Fri 11/21/2008 2:36 PM To: Jackie M O'Connor; Cheryl Crowder Cc: [EMAIL PROTECTED]; Histonet Subject: RE: [Histonet] Sakura tape problem How do you do this with the tissue attached to the tape? Just curious... j -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Jackie M O'Connor Sent: Friday, November 21, 2008 2:32 PM To: Cheryl Crowder Cc: [EMAIL PROTECTED]; Histonet Subject: Re: [Histonet] Sakura tape problem I would remove the tape with acetone and clear in xylene, then recoverslip with conventional coverslips. I've seen problems with refractivity with the tape, anyway. Happy Turkey Day. Jackie O' Cheryl Crowder [EMAIL PROTECTED] Sent by: [EMAIL PROTECTED] 11/21/2008 01:20 PM To Histonet [EMAIL PROTECTED] cc Subject [Histonet] Sakura tape problem Hi - I have just been given a box (100 slides) cover slipped with Sakura tape. All the tapes have been loosened from the slides with the tissue attacked to it. The tape has also curled (arched). What is the best method for reattaching the tapes to the slides so they can be viewed again. Or can it? The pathologists are really upset over these slides as they are for continuing education and cannot be replaced. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet === The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. === ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thanks for help on desperately seeking help for cryosectioning and further question
Dear All, thank you so much for your suggestions on improving my cryosectioning results. I tried several of your suggestions, however, the quality of my sections did'nt improve so much. I get perfect sections as long as I only cut the OCT embedding media. As soon as I reach embryonal tissue, only the tissue starts to tear up. In some of the embryos I loose almost all tissue. In my opinion this can only be caused by the fixation of the mouse embryos. Here is our protocol: 1. Fixation in 4% PFA overnight at 4°C 2. 30% sucrose solution at 4°C till embryo sinks to bottom of the tube 3. transfer to 1:1 mix of 30% sucrose solution and OCT for 4h at 4°C 4. transfer to 100% OCT and freeze quickly in isopentane (cooled down with dry ice for approx. 30min before use). 5. store at -80°C Is there something wrong? Should I follow a different protocol`? How long can you store OCT-embedded tissue? Are 6 months too long? Thanks so much for your help and best regards, Barbara -- Dipl. Biol. Barbara Schormair PhD student Institute of Human Genetics Tel.: 0049-89-3187-4097 Fax:0049-89-3187-3474 e-Mail: [EMAIL PROTECTED] Helmholtz Zentrum München German Research Center for Environmental Health (GmbH) Ingolstaedter Landstraße 1 D-85764 Neuherberg Germany Chairman of Supervisory Board: MinDir Dr. Peter Lange Board of Directors: Prof. Dr. Günther Wess and Dr. Nikolaus Blum Register of Societies: Amtsgericht München HRB 6466 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] problem with NSH website???
The office is closed for the holiday, as I have tried calling them. I also tried to use the website over the holiday and had the same issues that Chris van der Loos had. I have faith - all will be restored by Monday afternoon! ;-) Hope everyone had a happy and festive Thanksgiving! Vikki On Fri, Nov 28, 2008 at 11:33 AM, Kristen Yaros [EMAIL PROTECTED] wrote: It was happening to me last night as well. I wonder if a server or something is down... somewhere. Kristen On Fri, Nov 28, 2008 at 8:31 AM, Pamela Marcum [EMAIL PROTECTED] wrote: I am not sure what is going on Chris. I just tried it and I got the same result you did going to Isovers etc. Obviously something is very wrong and they need to correct it. I will forward you e-mail and my answer to them so they know it is not just a problem for Europe. I assume they monitor HistoNet - I could be wrong. Pam Marcum - Original Message - From: C.M. van der Loos [EMAIL PROTECTED] To: histonet netserver histonet@lists.utsouthwestern.edu Sent: Friday, November 28, 2008 4:02:21 AM GMT -05:00 US/Canada Eastern Subject: [Histonet] problem with NSH website??? Dear All,Desperately I tried to submit my abstracts for the NSH convention. Using the links for submission in the email we got from Aubrey Wanner/Kim Simmons early November, the browser goes to Isovera.com a domain for sale in Germany, provided by Sedo Domain Parking. Obviously something that has nothing to do with NSH. The same problems occurs at the NSH website. You can go to the NSH website as normal, but clicking on 'meetings/event' and selecting 'symposium/convention' one is re-directed to that same crap. What is going on here??? Does this problem also occurs with others???Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Kristen Yaros, HT (ASCP)CM Histotechnology Society of Delaware Correspondence Secretary [EMAIL PROTECTED] ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Help with protocols
If the CC1 standard 32 with amp, 42 degrees does not work then try using protease 1 4-6 minutes on it. On Fri, Nov 28, 2008 at 12:35 PM, Lena Spencer [EMAIL PROTECTED]wrote: Hi All: I have been working up the PMS2 and MLH2 mismatched repair genes and I am not satisfied with my results. I am using the Cell Marque antibodies and the Ventana XT using CC1, mild, standard and I have tried the antibodies with block and amplification, and antibody times from 32 min- 1 hour and the stain is not satisfactory. Anyone who has these antibodies in their lab and would like to share your protocols, I would be grateful for your help. The MSH2 and MSH6 are working great. Lena Spencer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The Unknown HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ventana Benchmark XTs
They are switching to a more reliable manufacturer. They are being proactive and switching them before they fail down the road. My XT has been a workhorse but we know certain peoples opinions about the company on here. I will take it any day over the rest. On Wed, Nov 26, 2008 at 5:29 PM, Burton, Lynn [EMAIL PROTECTED]wrote: We have had some trouble but they are planning to replace them. We received a letter that they had changed vendors for parts and found they need to switch back. They did replace one section earlier when it stopped working completely. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 From: [EMAIL PROTECTED] on behalf of Tracey Lenek Sent: Wed 11/26/2008 10:59 AM To: histonet@lists.utsouthwestern.edu Cc: Martin Trotter; Joanna Bartczak-McKay Subject: [Histonet] Ventana Benchmark XTs Hi, We have had on-going thermal slide pad issues with our XTs since they were installed in February. The slide tray assemblies have been replaced not once but twice and we are still having inconsistent results with the temp verifier slides. Has anyone experienced the same issues with this instrumentation? Thanks Tracey Lenek Tech III - Anatomic Pathology Calgary Laboratory Services 403-770-3588 This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The Unknown HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ventana Benchmark XTs
I don't know how much the opinion of The Unknown HT(ASCP) counts. You aren't with Ventana I hope. Mark On Fri, Nov 28, 2008 at 11:51 AM, Histonet Alias [EMAIL PROTECTED]wrote: They are switching to a more reliable manufacturer. They are being proactive and switching them before they fail down the road. My XT has been a workhorse but we know certain peoples opinions about the company on here. I will take it any day over the rest. On Wed, Nov 26, 2008 at 5:29 PM, Burton, Lynn [EMAIL PROTECTED] wrote: We have had some trouble but they are planning to replace them. We received a letter that they had changed vendors for parts and found they need to switch back. They did replace one section earlier when it stopped working completely. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 From: [EMAIL PROTECTED] on behalf of Tracey Lenek Sent: Wed 11/26/2008 10:59 AM To: histonet@lists.utsouthwestern.edu Cc: Martin Trotter; Joanna Bartczak-McKay Subject: [Histonet] Ventana Benchmark XTs Hi, We have had on-going thermal slide pad issues with our XTs since they were installed in February. The slide tray assemblies have been replaced not once but twice and we are still having inconsistent results with the temp verifier slides. Has anyone experienced the same issues with this instrumentation? Thanks Tracey Lenek Tech III - Anatomic Pathology Calgary Laboratory Services 403-770-3588 This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The Unknown HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ventana Benchmark XTs
no, they usually contact the CEO's of the labs to get their employees to stop ragging on the company. Then again, you never know who is lurking in the winds. JTT - Original Message - From: Mark Tarango [EMAIL PROTECTED] To: Histonet Alias [EMAIL PROTECTED] Cc: histonet@lists.utsouthwestern.edu; Martin Trotter [EMAIL PROTECTED]; Tracey Lenek [EMAIL PROTECTED]; Burton,Lynn [EMAIL PROTECTED]; Joanna Bartczak-McKay [EMAIL PROTECTED] Sent: Friday, November 28, 2008 2:10 PM Subject: Re: [Histonet] Ventana Benchmark XTs I don't know how much the opinion of The Unknown HT(ASCP) counts. You aren't with Ventana I hope. Mark On Fri, Nov 28, 2008 at 11:51 AM, Histonet Alias [EMAIL PROTECTED]wrote: They are switching to a more reliable manufacturer. They are being proactive and switching them before they fail down the road. My XT has been a workhorse but we know certain peoples opinions about the company on here. I will take it any day over the rest. On Wed, Nov 26, 2008 at 5:29 PM, Burton, Lynn [EMAIL PROTECTED] wrote: We have had some trouble but they are planning to replace them. We received a letter that they had changed vendors for parts and found they need to switch back. They did replace one section earlier when it stopped working completely. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 From: [EMAIL PROTECTED] on behalf of Tracey Lenek Sent: Wed 11/26/2008 10:59 AM To: histonet@lists.utsouthwestern.edu Cc: Martin Trotter; Joanna Bartczak-McKay Subject: [Histonet] Ventana Benchmark XTs Hi, We have had on-going thermal slide pad issues with our XTs since they were installed in February. The slide tray assemblies have been replaced not once but twice and we are still having inconsistent results with the temp verifier slides. Has anyone experienced the same issues with this instrumentation? Thanks Tracey Lenek Tech III - Anatomic Pathology Calgary Laboratory Services 403-770-3588 This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The Unknown HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
