Re: [Histonet] double staining on the ventana XT

2009-01-23 Thread Piero Nelva
We also run a p63/HMCK/p504s dual stain cocktail on prostate cores.  Easy to 
set up and results look great.


THe Kappa/Lambda dual stain works, too.  I find the red chromagen (lambda) 
in this setting is a little less precise, tho'.


REgards

Piero Nelva
Anatomical PAthology
Monash Medical Centre
- Original Message - 
From: Gudrun Lang gu.l...@gmx.at

To: histoi...@comcast.net
Cc: histonet@lists.utsouthwestern.edu
Sent: Friday, January 23, 2009 3:56 AM
Subject: AW: [Histonet] double staining on the ventana XT


Hi Jennifer,
we perform the doublestain routinly. The preferred protocol is CK HMW/p504s
on prostata biopsies.
The protocol is easy to establish and the staining results are very good.
The run lasts about 4 hours and we do it overnight. You need the Red and the
DAB ultraview kit. No extra reagens.
Gudrun

-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von
histoi...@comcast.net
Gesendet: Donnerstag, 22. Jänner 2009 01:41
An: Histonet@lists.utsouthwestern.edu
Betreff: [Histonet] double staining on the ventana XT


Greetings,
We are looking into doing some immuno double staining. I searched the
archives and found a few similar inquiries but no responses. Anyone with
experiences to share I would love to hear what you have to say. Everything
from what not to do to what works well for you.
Many Thanks,
Jennifer



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RE: [Histonet] RE: Prion Contamination

2009-01-23 Thread Bartlett, Jeanine (CDC/CCID/NCZVED)
All,

The formic acid is a post-fixation step.

 
http://www.cdc.gov/od/ohs/biosfty/bmbl4/bmbl4s7d.htm#Table%205.%20Tissue
%20preparation


Jeanine Bartlett, BS, HT(ASCP)QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590 
jeanine.bartl...@cdc.hhs.gov


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle
weaver
Sent: Thursday, January 22, 2009 6:46 PM
To: sal...@exchange.hsc.mb.ca; Histonet
Subject: RE: [Histonet] RE: Prion Contamination


I just researched this recently for procedure writing. I referenced an
article from the CDC that made mention of specific handling guidelines
for histology, and also specifically the formic acid treatment step. I
do not have the article in front of me, but by my recollection I believe
that it stated that the formic acid was a post-fixation step. I found
this article relatively easily by using the search field for CJD from
the CDC website. You can check my recollection here:
http://www.cdc.gov/od/ohs/biosfty/bmbl5/sections/SectionVIIIH-PrionDisea
ses.pdf
Thanks
Joelle Weaver HTL(ASCP)
 Date: Thu, 22 Jan 2009 13:21:25 -0600 From: 
 sal...@exchange.hsc.mb.ca To: tbr...@holyredeemer.com Subject: 
 [Histonet] RE: Prion Contamination CC: 
 histonet@lists.utsouthwestern.edu  Hi, I was reading your email of 
 Jan. 15/09 on the subject of Prion Contamination. In it you had 
 made the following statement, which I was curious as to where this 
 information had come from.  the procedure to inactivate using formic

 acid is followed before fixation and processing. If the tissue is 
 fixed (formalin or other common fixatives) then you would be actually

 fixing the prion's ability to NOT be inactivated.  I had read 
 again the WHO Guidelines on CJD that you had a link to  could find 
 no mention of the fact that the formic acid step for de-activating 
 the prions had to be done before any fixation. The WHO instructions: 
 P. 18 8.2.2 Histopathological examination: states formic acid 
 treatment consists of placing small pieces of fixed tissue, no more 
 than 4 to 5 mm thick, in 50 to 100 ml of 95% formic acid for an hour.

 I have been dealing with CJD brains for many years always following 
 the CDC, WHO  Health Canada guidelines but have never read any 
 studies that had indicated that the fixing in formalin made the 
 treatment in formic acid ineffective. If you have any further 
 information I would really appreciate seeing it.  Thank you  S. 
 Allen sal...@hsc.mb.ca 
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RE: [Histonet] Re: Xylene substitute

2009-01-23 Thread Blazek, Linda
Dear Bob Richmond, our honorable Samurai Pathologist.  The consensus of opinion 
around here is that you have been spending too much time smelling formalin!  We 
can't figure out how you get the smell of ether from Formula 83!  Most of us 
here are old enough to remember the smell of ether but don't equate it with 
Formula 83.

Respectfully,
Linda

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email: lbla...@digestivespecialists.com

 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond
Sent: Thursday, January 22, 2009 9:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Xylene substitute

Questions about xylene substitutes: This has been discussed a good
many times on Histonet.

There are two classes of xylene substitutes:

Limonene and turpentine are terpenes. Several brands; AmeriClear was
mentioned. Limonene is prepared by steam distillation of orange peels.
It has a strong citrus smell variously described as pleasant,
overwhelming, disgusting, and allergenic, and cannot be made odorless.
It is not very toxic, is not easily set afire, and is to a degree
biodegradable. It cannot be distilled. With America's declining citrus
industry, this once cheap product has become considerably more
expensive.

Aliphatic solvents are now in much more widespread use. They are more
expensive than xylene, but can be recovered by distillation. Most of
them are odorless, at least to my nose. They are not very toxic. Some
have much lower flash points than others, so that fire hazard varies
considerably. They are not easiy biodegradable. Different brands
differ considerably in chemical and physical properties, and
distillation routines for one brand cannot be used with another brand.

Richard Allan's Clear-Rite 3 may be the most widely used aliphatic.
ANATECH's Pro-Par was mentioned in this correspondence, and is very
meticulously described on their Web site.

I hadn't heard of CBG Biotech's Formula 83 before. Slightly different
from ordinary aliphatics, it is described as a naphthenic hydrocarbon
blend (cycloalkane). It is described as smells  like ether, and I
would be concerned about its very low flash point (45 F, below room
temperature, as described in the MSDS).

When you mention who makes a commercial product, please tell us who
makes it, and read the Materials Safety Data Sheet (MSDS) at the
product's Web site and see what you can find out what's in it.

I have no commercial connection with any product I've mentioned.

Bob Richmond
Samurai Pathologist
Knoxville TN

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[Histonet] Cytology Autostainers

2009-01-23 Thread Greg Dobbin
Hi Folks,
What's out there? What's hot and what's not? Anyone using their
cytology autostainer for histology special stains? Welcome all input
(including vendors).
Cheers!
Greg

Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PEC1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

I find that the harder I work, the 
more luck I seem to have.
- Thomas Jefferson


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[Histonet] RELIA Special Job Alert Brand New Management and Supervisory Opportunities 1-23-09

2009-01-23 Thread Pam Barker
Hi Histonetters,

I have several brand new exciting opportunities for experienced
Managers,and Supervisors  in hospital and private lab environments in
several locations nationwide.  These are some of the premier employers
in the United States. 

The positions are of course full time and permanent. 
Here are my BRAND NEW JOBS:

Histology Supervisor Night Shift – Ft. Lauderdale, FL
Histology Supervisor – Atlanta, GA

 

Here are some of my other leadership positions:
Histology Manager – Maryland, West of DC
Research Histology Manager – Boston, MA
Histology Supervisor - Los Angeles, CA



If you would like more information or know of someone else who might be
interested, please contact me at rel...@earthlink.net or 866-607-3542.
I am available to discuss the opportunity at your convenience including
after hours. Thanks-Pam

 

New for 2009 RELIA is offering a $500 referral bonus for anyone you
refer and I place and a $500 hiring bonus if I place you!

 

There are a lot of recruiters out there right now trying to work with
histo techs and I appreciate your support and respect your needs.
Remember I offer over 25 years of experience as a recruiter and for over
6 years I have dedicated my practice solely to placing histology
professionals like you.  


Thank You! 
 
Pam Barker
President
RELIA 
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net mailto:rel...@earthlink.net
http://home.earthlink.net/~relia1
www.myspace.com/pamatrelia http://www.myspace.com/pamatrelia
 
 
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Re: [Histonet] Re: Xylene substitute

2009-01-23 Thread Victor Tobias
In defense of the good Samurai, he was using a comment posted by someone 
else that the product smelled like ether.


Victor

Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
vic...@pathology.washington.edu
206-598-2792
206-598-7659 Fax
=
Privileged, confidential or patient identifiable information may be
contained in this message. This information is meant only for the use 
of the intended recipients. If you are not the intended recipient, or 
if the message has been addressed to you in error, do not read, 
disclose, reproduce, distribute, disseminate or otherwise use this 
transmission. Instead, please notify the sender by reply e-mail, and 
then destroy all copies of the message and any attachments.




Blazek, Linda wrote:

Dear Bob Richmond, our honorable Samurai Pathologist.  The consensus of opinion 
around here is that you have been spending too much time smelling formalin!  We 
can't figure out how you get the smell of ether from Formula 83!  Most of us 
here are old enough to remember the smell of ether but don't equate it with 
Formula 83.

Respectfully,
Linda

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email: lbla...@digestivespecialists.com

 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond
Sent: Thursday, January 22, 2009 9:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Xylene substitute

Questions about xylene substitutes: This has been discussed a good
many times on Histonet.

There are two classes of xylene substitutes:

Limonene and turpentine are terpenes. Several brands; AmeriClear was
mentioned. Limonene is prepared by steam distillation of orange peels.
It has a strong citrus smell variously described as pleasant,
overwhelming, disgusting, and allergenic, and cannot be made odorless.
It is not very toxic, is not easily set afire, and is to a degree
biodegradable. It cannot be distilled. With America's declining citrus
industry, this once cheap product has become considerably more
expensive.

Aliphatic solvents are now in much more widespread use. They are more
expensive than xylene, but can be recovered by distillation. Most of
them are odorless, at least to my nose. They are not very toxic. Some
have much lower flash points than others, so that fire hazard varies
considerably. They are not easiy biodegradable. Different brands
differ considerably in chemical and physical properties, and
distillation routines for one brand cannot be used with another brand.

Richard Allan's Clear-Rite 3 may be the most widely used aliphatic.
ANATECH's Pro-Par was mentioned in this correspondence, and is very
meticulously described on their Web site.

I hadn't heard of CBG Biotech's Formula 83 before. Slightly different
from ordinary aliphatics, it is described as a naphthenic hydrocarbon
blend (cycloalkane). It is described as smells  like ether, and I
would be concerned about its very low flash point (45 F, below room
temperature, as described in the MSDS).

When you mention who makes a commercial product, please tell us who
makes it, and read the Materials Safety Data Sheet (MSDS) at the
product's Web site and see what you can find out what's in it.

I have no commercial connection with any product I've mentioned.

Bob Richmond
Samurai Pathologist
Knoxville TN

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[Histonet] Legal Question

2009-01-23 Thread Jessica Piche


Hi, We have an antibody that we ordered in error. We would like to see if 
another lab would like to purchase it from us. Is that legal to ask this on the 
Histonet? Thanks!
 

Jessica Piche-Grocki, HT(ASCP)
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Re: [Histonet] Legal Question

2009-01-23 Thread Jackie M O'Connor
Or you can just ask the supplier to take it back.





Jessica Piche jessgro...@yahoo.com 
Sent by: histonet-boun...@lists.utsouthwestern.edu
01/23/2009 11:38 AM

To
histonet histonet@lists.utsouthwestern.edu
cc

Subject
[Histonet] Legal Question








Hi, We have an antibody that we ordered in error. We would like to see if 
another lab would like to purchase it from us. Is that legal to ask this 
on the Histonet? Thanks!
 

Jessica Piche-Grocki, HT(ASCP)
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[Histonet] (no subject)

2009-01-23 Thread MARY T HODGES

Good day all,
 
  Thank you so much for your input on decal billing. I have another questain? 
This one is a little strange.
When you put in charges as a prelimary charge ... do you over bill or under 
bill? I have always stayed under while putting in charges and then audit the 
doctors charges the next day. example
colon segment other then tumor... bill   88307
the next day tumor was found now it is bumped up to  88309
Thanks again for all your help.
Tere Hodges
St. Mary's Hospital
Tucson, Az. 
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Re: [Histonet] Starfrost slides

2009-01-23 Thread Merced Leiker
I have used them almost daily for a year or more now and have had no 
problems with the slides giving off any autofluorescence in 
immunofluorescence applications.



--On Thursday, January 22, 2009 2:56 PM -0500 Nancy  Lemke 
ns...@neuro.hfh.edu wrote:



Has anyone using Starfrost slides (7255) from Mercedes had any problems
with autofluoresence when doing IF? Thanks in advance.
Nancy Lemke
Research Coordinator
Hermelin Brain Tumor Center
Henry Ford Hospital
Detroit


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School of Medicine and Biomedical Sciences
State University of New York at Buffalo
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Ph: (716) 829-6033
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[Histonet] LabVision/Dako Autostainer

2009-01-23 Thread Della Speranza, Vinnie
We have earlier generation Dako Autostainers and are evaluating a current 
generation LabVision stainer. New or old we have experienced occasions when 
this instrument design has failed to dispense reagent. This seems to occur 
randomly or if there is a pattern we haven't figured it out. I've recently 
learned that some owners of the Biocare Nemesis which is essentially the same 
instrument have also encountered this problem.

The arm will travel to the correct slide but nothing dispenses from the probe, 
then the arm moves on its way. 

I'd appreciate learning if others have experienced this problem and if a 
solution to the problem was discovered I hope you'll share it with me.



Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
 


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Re: [Histonet] Starfrost slides

2009-01-23 Thread Jennifer Harvey
From what I understand they are made with green glass. Green glass is a less
superior glass than white and you can have fluorescence issues. You should
only use white glass for fluorescence applications. You can tell by looking
at the slides in the box on their sides.
The Mercedes slides (7255) are fine for HE and regular immuno. I use them
for one of my researchers to help with his costs and have been quite happy
with them. I do say that I do use the coverslips (mer2450) and they are to
be made of green glass also. I am not sure of this since when you look at
them on there side in the box they don't seem green. I have used them on
fluorescent work and haven't noticed a problem.
I hope this helps. I had the same question when I was trying to save money
on slide costs. 


 Jennifer Harvey, HT(ASCP) QIHC
 Vanderbilt Vision Research Center
 Core Histologist
 RM 8105 MCE North Tower
 1215 21st Ave. South
 Nashville, TN  37232-8808
 Phone 615-936-1486
  


On 1/22/09 1:56 PM, Nancy  Lemke ns...@neuro.hfh.edu wrote:

 Has anyone using Starfrost slides (7255) from Mercedes had any problems with
 autofluoresence when doing IF?
 Thanks in advance.
 Nancy Lemke
 Research Coordinator
 Hermelin Brain Tumor Center
 Henry Ford Hospital
 Detroit
 
 
 ==
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[Histonet] Ventana Basic V-Red Kit

2009-01-23 Thread Cindy . J . Chard-Bergstrom
How many labs out there are still using the basic V-Red Kit from Ventana? 
Have you had any problems using the enhanced or the ultraview red on 
animal tissues?

Cindy
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RE: [Histonet] LabVision/Dako Autostainer

2009-01-23 Thread CHRISTIE GOWAN

Hey Vinnie,
We have 2 lab visions and we also experienced this problem. They came in and 
changed the level sensors and we have not had the problem since. 
Christie
UAB Hospital
Birmingham, AL From: del...@musc.edu To: histonet@lists.utsouthwestern.edu 
Date: Fri, 23 Jan 2009 15:13:36 -0500 Subject: [Histonet] LabVision/Dako 
Autostainer   We have earlier generation Dako Autostainers and are evaluating 
a current generation LabVision stainer. New or old we have experienced 
occasions when this instrument design has failed to dispense reagent. This 
seems to occur randomly or if there is a pattern we haven't figured it out. 
I've recently learned that some owners of the Biocare Nemesis which is 
essentially the same instrument have also encountered this problem.  The arm 
will travel to the correct slide but nothing dispenses from the probe, then the 
arm moves on its way.   I'd appreciate learning if others have experienced 
this problem and if a solution to the problem was discovered I hope you'll 
share it with me.Vinnie Della Speranza Manager for Anatomic Pathology 
Services Medical University of South Carolina 165 Ashley Avenue Suite 309 
Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974   
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[Histonet] RE: Prion Contamination-Post Fixation

2009-01-23 Thread Terri Braud
Ooops, my bad!..you are both right!  Another reason that prion contaminated 
tissues should be left to the experts.  And for the rest of us, just read the 
links for yourself, and try to be safe!
Thanks, Terri
Terri L. Braud, HT(ASCP)



My recollection I believe that it stated that the formic acid was a 
post-fixation step. 
Joelle Weaver HTL(ASCP)

All,
The formic acid is a post-fixation step.
Jeanine Bartlett, BS, HT(ASCP)QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590 
jeanine.bartl...@cdc.hhs.gov

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[Histonet] methyl green - multiple labelling

2009-01-23 Thread anjan kumar

hi everybody,
  has anyone tried methyl green as
counter stain in multiple label immunohistochemistry. i tried one but i
got a dull back ground when i took a snap through Image pro plus 5.1.
regards,
anjan.
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Re: [Histonet] methyl green - multiple labelling

2009-01-23 Thread TF
yes methyl blue  methyle green are too dim..
try neutral red...?

it seems fast green/light green might lead to intense staining? I am not sure, 
you may test it.



2009-01-24 



TF 



发件人: anjan kumar 
发送时间: 2009-01-24  13:11:19 
收件人: triple immunohistochem 
抄送: 
主题: [Histonet] methyl green - multiple labelling 
 
hi everybody,
  has anyone tried methyl green as
counter stain in multiple label immunohistochemistry. i tried one but i
got a dull back ground when i took a snap through Image pro plus 5.1.
regards,
anjan.
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