RE: [Histonet] Tube station
I stayed near Russell Square station but liked Victoria despite the hustle and bustle ... Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Friday, February 27, 2009 4:41 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tube station I always preferred King's Cross myself, but I was only visiting... Claire PS, Don't confuse us poor Americans. We're used to the 'L', etc. From: histonet-boun...@lists.utsouthwestern.edu on behalf of Edwards, R.E. Sent: Thu 2/26/2009 3:32 AM To: 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tube station There is only one tube station, Mornington Crescent!!. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] log book
The OR delivery all the specimens to us. We do not go to the OR at all for specimens. Whom ever brings the OR specimens must lay out all specimens with the slips. Any case that is missing a specimen, we reject the case and the OR runner takes it back to the people that work that case to figure out what they did wrong. If we accept a case, we time/date stamp as we sign the OR book. If it is after hours the OR delivers the specimens to the clinical specimen processing area and they sign for the specimens, then they take them to the Histology lab. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy Gorham [gorh...@verizon.net] Sent: Sunday, March 01, 2009 8:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] log book Good Monday Morning, We had a serious incident Friday with O.R. My aide went down to get the specimens from O.R. about 9am. (which were left overs from the night before). She did not stamp in the specimens before she left. When I had time to stamp them in and record them in the log book I discovered that the colon was not there. Two other specimens from that patient where in the bag but no colon. So I went down to O.R. to see where it was. Of course no one knows what happened to the colon. The doctors are furious by all means. Now the O.R. thinks the path lab screwed up. So my questions is how do others log in the specimens as they come into the lab. We have 2 couriers that brings specimens when we are not in the lab from other hospitals. How do you make sure that whom ever brings the specimens actually brings the ones they say they do? Do you have a log book that every specimen that is brought into the lab is written down by the person who brings it in? Right now we have a log book but it is written in as we are accessing the specimens. So the specimens may have been there overnight. We are a very small lab and we do almost everything by hand including writing in the log book. Someday we want to be able to scan by bar codes but right now we can not do that. Thanks for any help you can give me. Kathy Gorham, H.T. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cathy Crumpton is out of the office.
I will be out of the office starting Mon 03/02/2009 and will not return until Thu 08/27/2009. If you have any histology concerns please route them to Connie Basinski during my absence. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: specimen tracking from the OR
We are a small hospital lab, too. There is a log book in the OR, where specimens are placed for Histo pick up. The circulating nurse puts a Patient sticker and handwrites all specimens removed from the patient, regardless of what they are, and whether or not they have been walked down, tubed, or waiting pickup. We make sure that all specimens we remove are listed in the book, then sign/date/time for them. Any specimen left for us and not in the book, or vice versa, we bring to the attention of the OR control desk. If we make a run and there are no specimens to pick up, then we still sign and date/time the log book as no specimens. Any specimens brought to the department are checked before accepting. Crude and time consuming, yesaccurate, too. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax 6. log book (Kathy Gorham) Message: 6 Date: Sun, 01 Mar 2009 17:15:47 -0800 From: Kathy Gorham gorh...@verizon.net Subject: [Histonet] log book Good Monday Morning, We had a serious incident Friday with O.R. My aide went down to get the specimens from O.R. about 9am. (which were left overs from the night before). She did not stamp in the specimens before she left. When I had time to stamp them in and record them in the log book I discovered that the colon was not there. Two other specimens from that patient where in the bag but no colon. So I went down to O.R. to see where it was. Of course no one knows what happened to the colon. The doctors are furious by all means. Now the O.R. thinks the path lab screwed up. So my questions is how do others log in the specimens as they come into the lab. We have 2 couriers that brings specimens when we are not in the lab from other hospitals. How do you make sure that whom ever brings the specimens actually brings the ones they say they do? Do you have a log book that every specimen that is brought into the lab is written down by the person who brings it in? Right now we have a log book but it is written in as we are accessing the specimens. So the specimens may have been there overnight. We are a very small lab and we do almost everything by hand including writing in the log book. Someday we want to be able to scan by bar codes but right now we can not do that. Thanks for any help you can give me. Kathy Gorham, H.T. - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] specimens
The first problem was with the tech who removed thespecimens from the OR without checking to make certain everything was included. She/he should never have left the OR without responding to the issue and that would have taken the blame off of the histotechs. We have OR bring specimens to us and sign in with a label as used on the container, initial and date/time everything. The techs accepting specimens have to sign off and accept responsibility at that point. When we did go to the OR, a few yaers ago, we had the same logbook in place there. The OR personnel had to sign everything in and when we picked the tissue up, we checked each specimen off in the logbook with our initials and time/date. Same process, only in reverse. Once anyone initialed the logbook, it was that person's responsibility as to the specimen integrity. Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HIER
Hi histonetters We have been talking a lot about how to retrieve your epitopes using a microwave in the best possible way. We haven't come to an agreement and people in my lab both argue that temperature and pH are important issues. Without a doubt both factors are important for a proper retrieval, but if you have to focus on one of the factors, which would you consider the most important? Temperature or pH? The issue is mainly longer incubations of the slides in boiling buffer. The buffer is evaporating and the solution/buffer gets less and less pH neutral and you need to top up with dH2O. However, if you boil with reduced intensity, less evaporation will occur and the pH will remain more stable. Do you have any suggestions or comments to this issue? Looking forward to your replies. Cheers Casper ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: log book
-Original Message- -Original Message- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Hi Kathy, we are a small private lab who has the contract with the local hospital. What we have is a log book in the O.R. where the nurses have to enter the specimens for pick up; our courier than signs off on the log in the O.R. that he/she has picked up the specimen. Each specimen is checked against the log before being transported to the lab. That way, if a specimen is missing, it can be taken up with the O.R. staff immediately. Hope that is helpful to you. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico Message: 6 Date: Sun, 01 Mar 2009 17:15:47 -0800 From: Kathy Gorham gorh...@verizon.net Subject: [Histonet] log book To: histonet@lists.utsouthwestern.edu Message-ID: 27e50df4f8434674b769ee3c1a469...@kathy83b707eca Content-Type: text/plain; charset=iso-8859-1 Good Monday Morning, We had a serious incident Friday with O.R. My aide went down to get the specimens from O.R. about 9am. (which were left overs from the night before). She did not stamp in the specimens before she left. When I had time to stamp them in and record them in the log book I discovered that the colon was not there. Two other specimens from that patient where in the bag but no colon. So I went down to O.R. to see where it was. Of course no one knows what happened to the colon. The doctors are furious by all means. Now the O.R. thinks the path lab screwed up. So my questions is how do others log in the specimens as they come into the lab. We have 2 couriers that brings specimens when we are not in the lab from other hospitals. How do you make sure that whom ever brings the specimens actually brings the ones they say they do? Do you have a log book that every specimen that is brought into the lab is written down by the person who brings it in? Right now we have a log book but it is written in as we are accessing the specimens. So the specimens may have been there overnight. We are a very small lab and we do almost everything by hand including writing in the log book. Someday we want to be able to scan by bar codes but right now we can not do that. Thanks for any help you can give me. Kathy Gorham, H.T. -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] distilled water
ummm...distilled water works best, unless you like cleaning out mineral deposit build-up and such... --On Monday, March 02, 2009 11:18 AM -0800 kristen arvidson arvidsonkris...@yahoo.com wrote: Are most people using distilled water in the water baths? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvienienced! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HIER
Both temperature and pH go hand in hand and it is very difficult, if not impossible, to select one over the other without experimental data. The thing is to just boil the container with the pH buffer and once it has boiled (usually in 20 minutes) take it out and leave it on the counter for another 20 minutes. The pH (from 6 to 8) will depend on the epitope that is going to be retrieve because some need pH6 and other pH8 and even higher (or lower). Seldom pH7 (neutral) is used. René J. --- On Mon, 3/2/09, Casper Hempel casperhem...@gmail.com wrote: From: Casper Hempel casperhem...@gmail.com Subject: [Histonet] HIER To: histonet@lists.utsouthwestern.edu Date: Monday, March 2, 2009, 1:52 PM Hi histonetters We have been talking a lot about how to retrieve your epitopes using a microwave in the best possible way. We haven't come to an agreement and people in my lab both argue that temperature and pH are important issues. Without a doubt both factors are important for a proper retrieval, but if you have to focus on one of the factors, which would you consider the most important? Temperature or pH? The issue is mainly longer incubations of the slides in boiling buffer. The buffer is evaporating and the solution/buffer gets less and less pH neutral and you need to top up with dH2O. However, if you boil with reduced intensity, less evaporation will occur and the pH will remain more stable. Do you have any suggestions or comments to this issue? Looking forward to your replies. Cheers Casper ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Distilled water in the baths?
We always used tap water with gelatin in the water bath for regular sectioning, but for IHC procedures we used distilled water without any gelatin. René J. --- On Mon, 3/2/09, kristen arvidson arvidsonkris...@yahoo.com wrote: From: kristen arvidson arvidsonkris...@yahoo.com Subject: [Histonet] Distilled water in the baths? To: histonet histonet@lists.utsouthwestern.edu Date: Monday, March 2, 2009, 2:10 PM Are people using tap water in their waterbaths? Has anyone noticed any problems with this? Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HIER
Casper: I have a reproducibly good method for HEIR for two Abs I routinely use (alpha smooth muscle actin in mouse liver and proliferating cell nuclear antigen (PCNA) in mouse liver). I use a microwave pressure cooker (Nordic Ware, tender cooker). Into the pressure cooker, I place 300mL of distilled water to keep the humidity high during the in my pressure cooker. I use a citrate buffer solution which contains 0.01M sodium citrate dihydrate and 0.04M citric acid monohydrate, pH 6.0. I place 500mL of this solution into a 1.5pt (710mL) 'servin' saver' container and set the filled container into the pressure cooker (into the distilled water). I slip my slides into an autostainer rack and place the rack on its side in the citrate buffer soln. I microwave the entire setup at 50% power (1100 watt microwave) for 20 minutes and then allow the cooker to cool down for 20 additional minutes. When I remove the cooker from the microwave, I open it on my bench and take the temperature of the citrate buffer. It is usually between 95-98C. After the 20 minute cool-down, the temperature of the citrate buffer is around 56-57C. Once completely cool, I test the pH of the citrate buffer to ensure that it is still at pH 6.0. (I have taken these measurements a total of 11 times.) My single failure when using this method of HIER was when the temperature did not get hot enough. I was rather naïve at the time and thought I would proceed with the staining and see what happened, assuming the pH was more important than the temperature. So much for that hypothesis. Mind you, I fully disclose that my experience is rather limited to the two staining protocols I have optimized, but I offer up that experience for your edification. Hope it helps! Kind regards: ---mtp Michele T. Pritchard, Ph.D. Research Associate Nagy Laboratory Department of Pathobiology/NE40 Lerner Research Institute Cleveland Clinic 9500 Euclid Avenue Cleveland, OH 44195 phone: 216.444.8613 fax: 216.636.1493 email: prit...@ccf.org Lab location: Lerner Research Institute NE4-214 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Casper Hempel Sent: Monday, March 02, 2009 1:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HIER Hi histonetters We have been talking a lot about how to retrieve your epitopes using a microwave in the best possible way. We haven't come to an agreement and people in my lab both argue that temperature and pH are important issues. Without a doubt both factors are important for a proper retrieval, but if you have to focus on one of the factors, which would you consider the most important? Temperature or pH? The issue is mainly longer incubations of the slides in boiling buffer. The buffer is evaporating and the solution/buffer gets less and less pH neutral and you need to top up with dH2O. However, if you boil with reduced intensity, less evaporation will occur and the pH will remain more stable. Do you have any suggestions or comments to this issue? Looking forward to your replies. Cheers Casper ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet === P Please consider the environment before printing this e-mail Cleveland Clinic is ranked one of the top hospitals in America by U.S. News World Report (2008). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Manual for Nikon 300 diaphot
Hello, Does anyone have a manual for a Nikon Diaphot 300? I found a previous similar request by Necat Yilmaz, and a positive response by Stacey Barick. Your help would be much appreciated! Thanks, Janet Janet Dertien Faculty Associate Texas Tech University Health Science Center Pharmacology Neuroscience 3601 4th Street / STOP 6592 Lubbock, TX 79430 806-743-2425 x236 806-743-2744 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HIER
In our experience, the heat is definitely more important...we retrieve epitopes ranging from cytoskeletal markers (Troponins, Myosin Heavy Chain,...) to nucler (PCNA) and others (c-kit, beta-catenin, vonWillebrand Factor-vWF). We use a steamer set to 30', just place the slides in flat on the bottom of the basket: encircle the sections with Pap pen first, then pipette some buffer on. I always test pH 6 and pH 9 but haven't seen a difference yet between the two with the epitopes we're unmasking.) We have this wicked vWF antibody from Calbiochem (PC313) that unmasks in PBS (pH7) in the steamer and binds very well on both paraffins and frozens. (And it does need retrieval - without retrieval it is gives very little stain). Ok, I digress!! But you get the point...that's from our experience, anyway! ~Merced --On Monday, March 02, 2009 7:52 PM +0100 Casper Hempel casperhem...@gmail.com wrote: Hi histonetters We have been talking a lot about how to retrieve your epitopes using a microwave in the best possible way. We haven't come to an agreement and people in my lab both argue that temperature and pH are important issues. Without a doubt both factors are important for a proper retrieval, but if you have to focus on one of the factors, which would you consider the most important? Temperature or pH? The issue is mainly longer incubations of the slides in boiling buffer. The buffer is evaporating and the solution/buffer gets less and less pH neutral and you need to top up with dH2O. However, if you boil with reduced intensity, less evaporation will occur and the pH will remain more stable. Do you have any suggestions or comments to this issue? Looking forward to your replies. Cheers Casper ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvienienced! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
