[Histonet] Tissue RE-processing
Hi to everybody, I have a problem with a recent processing tissue. Normally I process tissue that is very small such as 3mm thick of vessel rings or small cubes of myocardium. Couple of days ago we process a batch of myocardial sections that are really thick. I thought might be thick enough my protocol to perfuse completely. I was wrong it was too thick after the overnight protocol the sections were moist and soft. Of course I didn't even try to embedded them. Question, what can I do now? Can I reprocess them? Once they certain amount of wax in them can I put them again through the alcohols and xylenes and again through paraffin? Or is better just to dry them immersing them in alcohol? I just change the solutions of the processor so before actually going ahead and process them again I preferred to ask since I wouldn't like to get all the solutions dirty with wax with the need to rechange them once again. I appreciate your answers and help. Armando Tellez atel...@crf.org Pathology Department Cardiovascular Research Foundation Skirball Center for Cardiovascular Research 8 Corporate Drive Orangeburg, New York,10962 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 2 histology positions at UCLA
Hi all, We still have 2 positions available here at UCLA. The first is a histotech I position, a 50/50 clinical/research position working with us in the clinical lab as well as in the lab of one of our urologic pathology faculty (a great guy I might add). While not a requirement, if you have experience in whole mount prostate and/or manual IHC, FISH etc that would be wonderful! The salary range for the histotech position is $31.53-$37.68 per hour DOE. The second position is for a laboratory assistant/tech. This position involves staining, coverslipping, sending out slides, maintaining equipment, running the Ventana special stainers, answering phones etc. The salary range for the laboratory assistant/tech position is $19.56-$25.80 per hour DOE. Please feel free to contact me or check out our website https://jobs2.mednet.ucla.edu/css_external/CSSPage_Welcome.ASP . Both positions have been listed, and FYI the hours listed on the site for the HTLI position are incorrect. Hours have not been determined yet, but will more than likely will be first or second (day) shift. The Job number for the HTLI position is H49313 and the laboratory assistant/tech is H49330. Thank you! Noelle Noёlle Linke M.S., HTL(ASCP)QIHC Manager, Histology Services Department of Pathology Laboratory Medicine David Geffen School of Medicine at UCLA 10833 Le Conte Ave A3-172 Los Angeles, CA 90095 Phone: 310-825-7397 Pager: 97471 nli...@mednet.ucla.edumailto:nli...@mednet.ucla.edu IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Cassettes and Processing and Fixation ~ Oh My!
Laura, 1. In the past we used different colored cassettes for rush, non rush and special projects. Then we noticed that we still treated every case like it was retained/rush!! So we dropped back to one color for all our regular cases; microscreen cassettes for anything that might slip through and for our comminuted meat products. 2. Day (3 hours) run blocks are dumped in (i.e. not organized). We don't usually have any problems except with the tissues cut by one pathologist - who cuts too fast and sometimes too thick. But since I am here alone in the wee hours of the mornings to embed, I prefer my night (16 hrs run) blocks organized in neat 20 block rows and a lid placed on the basket. Our baskets do not have organizing spacers. We pack'em tight for long overnight runs! We have never had infiltration problems with them. We use pressure/vacuum (both VIP E300s) on all stations, all runs. 3. Our tissues are shipped overnight. So 99% of them are received nicely fixed. Exception: winter time-and tissues being shipped from the Northern states and Canada. If they are received in formalin and are under-fixed, after grossing, we transfer them to warm formalin until they go on the processor, then we give them 10 minutes on the processor with heat/vacuum/ pressure. All tissues arriving after 10 AM are held for overnight. Approximately 50% of cases are completed and diagnosis reported in 8-9 hours after arrival. The next 45% are completed in 24 hrs. The last 5% are completed within 72 hours from arrival. We started cay runs in 1978. We used an Ultra AutoTechnicon (with an agitating basket) followed several years later by the Fisher Histomatic (with an adjustable-speed stir bar in the bottom of the chamber):both processors had a direct method of stirring. We were able to process tissues in 2 hrs. When we began our search for a new processor in the 1990's, there were no processors with a stirring bar in the bottom of the processing chamber-our processing times increased to compensate for reduced agitation. Good luck in your quest to shorten processing and turn-around times. We have all struggled with the same questions. And through trial-and-error (or trial-by-fire!) ultimately found the times right for each lab's unique circumstances and problems. Histologically yours, Barbara Barbara Stancel, HTL(ASCP) Lead Technologist USDA, FSIS, EL, Pathology RRC, 950 College Station Road Athens, Georgia 30605 706-546-3698 or 706-546-3556 barbara.stan...@fsis.usda.gov No trees were hurt in the sending of this e-mail. However many electrons were severly inconvenienced! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for Darcy Peat
I worked with Darcy in Hawaii years ago, and I think someone said she may have moved to Washington State. Darcy, are you out there? Does anyone know where she is? Jackie O' ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Does anyone have an opinion on Distilled vs DI water? Is there a difference in quality? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] metal molds vs. disposable molds
Hello everyone, We have a debate going on about purchasing metal molds vs. disposable plastic molds. Which is better? Is the cost better in the long run to buy metal? Also, how does everyone clean their metal molds? Thanks Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line shar...@celligent.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] Tissue RE-processing
You can go back into warm xylen for one hour, that removes the paraffin. Then let the tissue in 2 changes of ethanol abs. , one hour each. That should be long enough for dehydration and hardening the tissue. Then go into xylen again for one hour, and then let it sit in paraffin for 2-3 hours. The duration in paraffin is rather long, because there's no vacuum, that helps infiltration. Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Armando Tellez Gesendet: Dienstag, 10. März 2009 14:02 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Tissue RE-processing Hi to everybody, I have a problem with a recent processing tissue. Normally I process tissue that is very small such as 3mm thick of vessel rings or small cubes of myocardium. Couple of days ago we process a batch of myocardial sections that are really thick. I thought might be thick enough my protocol to perfuse completely. I was wrong it was too thick after the overnight protocol the sections were moist and soft. Of course I didn't even try to embedded them. Question, what can I do now? Can I reprocess them? Once they certain amount of wax in them can I put them again through the alcohols and xylenes and again through paraffin? Or is better just to dry them immersing them in alcohol? I just change the solutions of the processor so before actually going ahead and process them again I preferred to ask since I wouldn't like to get all the solutions dirty with wax with the need to rechange them once again. I appreciate your answers and help. Armando Tellez atel...@crf.org Pathology Department Cardiovascular Research Foundation Skirball Center for Cardiovascular Research 8 Corporate Drive Orangeburg, New York,10962 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] metal molds vs. disposable molds
I agree. I also find you get a more level plane which makes trimming easier. We use both in our group and the individuals that use the disposable tend to use them multiple times before tossing them. I have not had to clean the metal ones I use in years but have heard you can toss them in the clean cycle of your tissue processor. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, March 10, 2009 12:00 PM To: histonet@lists.utsouthwestern.edu; Sharon Campbell Subject: Re: [Histonet] metal molds vs. disposable molds Metals molds are easier to work with and almost indestructible, but if you have money to throw to the air and patient and time in large supply, use plastic. René J. --- On Tue, 3/10/09, Sharon Campbell shar...@celligent.net wrote: From: Sharon Campbell shar...@celligent.net Subject: [Histonet] metal molds vs. disposable molds To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 10, 2009, 11:55 AM Hello everyone, We have a debate going on about purchasing metal molds vs. disposable plastic molds. Which is better? Is the cost better in the long run to buy metal? Also, how does everyone clean their metal molds? Thanks Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line shar...@celligent.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] metal molds vs. disposable molds
Metal molds herewe tried the plastic molds but the base would rise up creating an indentation in the block, we needed a flat surface. We wash our molds in an old processor, using the old baskets and running a xylene to alcohol to 95% EtOH run. Then we spread them on a towel to dry and spray them with a mold release. We still use the small plastic molds for the biopsies, however. The metal molds are great for cooling quickly. In another lab I worked in, we used all plastic molds - and to not have to wash the molds, just replace as needed, was heaven! I would recommend trying each and see what works for your lab. Janet L. Bonner, HTL (ASCP) Pathology Laboratory From: histonet-boun...@lists.utsouthwestern.edu on behalf of Sharon Campbell Sent: Tue 3/10/2009 11:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] metal molds vs. disposable molds Hello everyone, We have a debate going on about purchasing metal molds vs. disposable plastic molds. Which is better? Is the cost better in the long run to buy metal? Also, how does everyone clean their metal molds? Thanks Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line shar...@celligent.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet === The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. === ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] metal molds vs. disposable molds
Hi Sharon- I've been on your side of this quandry before. A couple of considerations: what kinds of tissue are you embedding? How many blocks/shift? What is the most important aspect(s) of the following for your situation? The pros and cons: Metal - pro cool fast easy to remove once cold the base doesn't concave on the bigger sizes quicker response to hot/cold (easier to warm/cool for tissue handling during embedding reembedding) Metal - con have to be cleaned (can set on side and pass thru processor though this will spark a raging debate on using a processor for cleaning--REALLY hot soapy water works well, too) expensive up front cost Plastic - pro square bottoms--full face sections with less trimming (great for prost bx) no need to clean can use more than one time to reduce cost but will crack eventually CLEAR--can see how things are oriented on the fly Plastic - con harder to pop (have to be all the way hardened or the bottom can stick in the well) longer to cool ongoing expense larger sizes can 'convex' depending on embedding medium shrinkage I'm sure the Histonetters can add many points to this list. It comes down to what do you need most on a weighted scale of pro and con? In the end, I usually choose to use both--keeps costs down and gives us options and once in a while when you get back-ordered on the plastic you're not forced to fold paper boats like we did 30 years ago!! Hope this helps! Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing Healthcare Professionals - One GREAT fit at a time 800.756.3309 --- On Tue, 3/10/09, Sharon Campbell shar...@celligent.net wrote: From: Sharon Campbell shar...@celligent.net Subject: [Histonet] metal molds vs. disposable molds To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 10, 2009, 8:55 AM Hello everyone, We have a debate going on about purchasing metal molds vs. disposable plastic molds. Which is better? Is the cost better in the long run to buy metal? Also, how does everyone clean their metal molds? Thanks Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line shar...@celligent.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] metal molds vs. disposable molds
I like my metal molds. They are neat, you get even heat transfer, and are easy to clean when they need it. I clean them by tossing them in some boiling water with an excess of cleaning powder (Alconox or Sparkleen) or any detergent you have on hand (make sure it doesn't get too bubbly and froth over though). Then I rinse them under hot top water, spread on paper towels to dry, spray with mold release, and stack in the oven. Merced --On Tuesday, March 10, 2009 11:55 AM -0400 Sharon Campbell shar...@celligent.net wrote: Hello everyone, We have a debate going on about purchasing metal molds vs. disposable plastic molds. Which is better? Is the cost better in the long run to buy metal? Also, how does everyone clean their metal molds? Thanks Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line shar...@celligent.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] metal molds vs. disposable molds
My issue with the plastic disposable molds is that when you need to re-embed and melt the block down, the mold will curl and lose its shape just enough to impede cutting the second time around. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Campbell Sent: Tuesday, March 10, 2009 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] metal molds vs. disposable molds Hello everyone, We have a debate going on about purchasing metal molds vs. disposable plastic molds. Which is better? Is the cost better in the long run to buy metal? Also, how does everyone clean their metal molds? Thanks Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line shar...@celligent.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CAP
Does any one have a procedure regarding : Handling of work load during instrument failure .? This would include, VIP,embedding ,etc... Behnaz Sohrab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] metal molds vs. disposable molds
And if you let the water cool, the paraffin will harden and you discard it without pouring down the drain... -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced Leiker Sent: Tuesday, March 10, 2009 12:22 PM To: Sharon Campbell; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] metal molds vs. disposable molds I like my metal molds. They are neat, you get even heat transfer, and are easy to clean when they need it. I clean them by tossing them in some boiling water with an excess of cleaning powder (Alconox or Sparkleen) or any detergent you have on hand (make sure it doesn't get too bubbly and froth over though). Then I rinse them under hot top water, spread on paper towels to dry, spray with mold release, and stack in the oven. Merced --On Tuesday, March 10, 2009 11:55 AM -0400 Sharon Campbell shar...@celligent.net wrote: Hello everyone, We have a debate going on about purchasing metal molds vs. disposable plastic molds. Which is better? Is the cost better in the long run to buy metal? Also, how does everyone clean their metal molds? Thanks Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line shar...@celligent.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] metal molds vs. disposable molds
We clean our metal molds in an ultra-sound cleaner. Fill it with warm water and a bit of dishcleaner, let it for 10 min. The paraffin easy goes off and swims on the surface. Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Sharon Campbell Gesendet: Dienstag, 10. März 2009 16:56 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] metal molds vs. disposable molds Hello everyone, We have a debate going on about purchasing metal molds vs. disposable plastic molds. Which is better? Is the cost better in the long run to buy metal? Also, how does everyone clean their metal molds? Thanks Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line shar...@celligent.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] metal molds vs. disposable molds
...which might be the better thing to do then...since it'll all be floating on the surface anyway and we don't want to risk clogging drain pipes with the wax once it cools... --On Tuesday, March 10, 2009 12:59 PM -0400 Weems, Joyce jwe...@sjha.org wrote: And if you let the water cool, the paraffin will harden and you discard it without pouring down the drain... -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced Leiker Sent: Tuesday, March 10, 2009 12:22 PM To: Sharon Campbell; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] metal molds vs. disposable molds I like my metal molds. They are neat, you get even heat transfer, and are easy to clean when they need it. I clean them by tossing them in some boiling water with an excess of cleaning powder (Alconox or Sparkleen) or any detergent you have on hand (make sure it doesn't get too bubbly and froth over though). Then I rinse them under hot top water, spread on paper towels to dry, spray with mold release, and stack in the oven. Merced --On Tuesday, March 10, 2009 11:55 AM -0400 Sharon Campbell shar...@celligent.net wrote: Hello everyone, We have a debate going on about purchasing metal molds vs. disposable plastic molds. Which is better? Is the cost better in the long run to buy metal? Also, how does everyone clean their metal molds? Thanks Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line shar...@celligent.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ci-Trol
Hello Everyone, Is anyone familiar with Ci-trol (citrated plasma) for making cell blocks? Someone had recommended a procedure using this, and I wanted to get some feedback on it. Thanks in advance! Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 2009 Tri-State Symposium
You are invited to join the circus and clown around with the histology societies of Wisconsin, Minnesota and Iowa as they host Big Top Histology the 2009 Tri-State Symposium, April 29-May 1, at The Madison Concourse Hotel, Madison, Wisconsin. The inclusive registration fee of $100 (+ state membership) covers workshops, seminars, lunches and our infamous costume banquet. The 2009 banquet theme will be Histology Under the Big Top; It's A 3 Ring Laboratory Circus Out There. CEU Approved Workshops Seminars Offered: Wednesday April 29th: Improving Lab Processes With the Use of Automatic Identification Thursday April 30th: The Basic Chemistry of Histological Staining, To Err Is Human; Patient Safety is Divine, An Introduction to Molecular Pathology, The Wild World of Neuropathology, Pretreatments-An Important Tool In IHC. Friday May 1st: Current Orthopedic Treatments for Pets, Mohs Surgery for Ocular Sebaceous Carcinoma Using ORO, Digital Imaging In Contract Research, Interpretation of Needle Core Biopsies in Evaluation of Non-Neoplastic Liver Diseases, Processing Schedules Revisited, Understanding CJD, Stars of the Silver Screen: Troubleshooting Basics for Silver Stains, IHC Basics Beyond. For further information contact any of the states representatives: Wisconsin: Maureen Decorah (deco...@wisc.edu) or Jean Mitchell (jmitch...@uwhealth.org) Minnesota: Ann Maruska (amaru...@mngastro.com) or Lois Rowe (rowe.l...@mayo.edu) Iowa: Judi Stasko (judith.sta...@ars.usda.gov) or Dave Cavanaugh (dcava...@yahoo.com) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CAP
This aspect has to be part of your contingency plan and each lab, with its own idiosyncrasies, has to prepare one, like what to do during/after a hurricane. René J. --- On Tue, 3/10/09, Behnaz Sohrab sohra...@ah.org wrote: From: Behnaz Sohrab sohra...@ah.org Subject: [Histonet] CAP To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 10, 2009, 12:49 PM Does any one have a procedure regarding : Handling of work load during instrument failure .? This would include, VIP,embedding ,etc... Behnaz Sohrab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] histological stain for pancreatic beta cells
Mallory Azan. René J. --- On Tue, 3/10/09, Michele Wich mw...@7thwavelabs.com wrote: From: Michele Wich mw...@7thwavelabs.com Subject: [Histonet] histological stain for pancreatic beta cells To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 10, 2009, 2:34 PM Can I please get some suggestions for histological stains that demonstrate pancreatic beta cells? I need something that would be highly specific without using an IHC method. Any advice is greatly appreciated! This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Need Histologists ASAP
New private AP lab in Phoenix, AZ., Top pay, great benefits, great hours, it's a new GI lab with state of the art equipment, please do not reply to this email, To submit a resume please apply to twincr...@bex.net _ Hotmail® is up to 70% faster. Now good news travels really fast. http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_70faster_032009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] validation documentation for processors
As all validations, you will have to process at least 25 pieces (CAP requires from 25 to 100) of tissue using your old and your new processor simultaneously. Make slides and later prepare HE and some HC and IHC procedures using both sets of slides and give them to as many pathologists as you can so they can select, without knowing which section comes from which processor, with only two options: either one section is better than the other, or both are equivalent for diagnostic purposes. Later your data should be analyzed statistically with the chi-square test or you could obviate the test if almost (more than 90%) of all the pairs are qualified as equivalent. René J. --- On Tue, 3/10/09, Joseph Fear fe...@bronsonhg.org wrote: From: Joseph Fear fe...@bronsonhg.org Subject: [Histonet] validation documentation for processors To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 10, 2009, 4:45 PM A question came up in our lab about validation documentation for our new processor. Does anyone have any creative feedback on how your lab documents validation of machines like processors and stainers? See below Joseph Fear 03/01/09 I'll post the question at histonet and see what i can find out about how other histo labs document validation of thier machines. I think with the peloris we ran test cases and Dr.Pearson checked the HE slides, but you're right that we don't have documentation of what cases were run and with JP's signiture for approval of validation. I can work on a general 'machine validation form' in excel and get back with you. -Joseph Virginia Rupert 02/20/09 3:44 PM In your searches, or past experience, do you know or can you find out how new instruments are validated? I don't think we have adequate documentation for the Peloris, with test cases, etc. But I also haven't found a document to use as a template for our purposes. Any ideas? Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cutting angle?
Hello Listers, I have inherited a Reichert-Jung 2030 microtome. The knife holder is a bit funky and hard to set the clearance angle. What angle do y'all use? I'm trying to get 4 or 5 degrees. Thanks, Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] User manual for Shandon HistoCenter 2
If you have one (or a xerox copy) to spare I would be very, very, very obliged. Thanks in advance! Yvan. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
