[Histonet] cryoprotection possible?
Dear Histonetters, I'm supposed to do a noradrenaline ihc on rat brains that have been snap frozen and afterwards glutaraldehyd fixed. While the morphology is okay in the test-tissue we used to establish the protocol, it was very poor in the tissue of the trial animals. It appeared mooth eaten and disrupted. I assume the brains were thawed and refrosted due to moving the samples between several cities. Does anyone know a method /a tip how I can save some proper morphology? I checked the archive and found sucrose as cryoprotectant, but this is supposed to do it while the tissue is frozen or is it possible to use it while the tissue thaws? Thank you all in advance for your patience and help, Frauke -- Dr. med. Frauke Neff AG Neurologische Therapieforschung Neurologie Philipps-Universität Marburg Rudolph-Bultmann Str. 8 35039 Marburg 06421/5866304 This message was sent using IMP, the Internet Messaging Program. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Hi, I just began to work with the methy methacrylate embedding plastic section. I am facing the difficulities to attach the sample on the glass slide. I have tried using Haupts adhesive coated slides and also the bond-rite slide to attach my 5micron sample. I am doing normal hematoxylin and eosin staining. The section always detach from the glass slide towards the end of the staining. I need to use the concentrated Haupts adhesive coated slide to reduce the chance for the section from lift up from the glass slide. However, I observed the high and dirty background after the staining. I noticed that some users are using Bond-rite slides to attach their samples. I tried this. However, the sections always lifted up towards the end of the staining. I am not too sure whether is my technique that giving me this kind of problem. I prewarmed the Bond-rite slides and put my sections in the 55C waterbath. Then I fished the section using the Bond-rite slide and lay it down on 55C heater. And finally in the oven incubator for more than 18 hours at 55C before staining... I would appreciate if there is any suggestion that can help to attach the section on the glass slide. Thank you very much! Yours sincerely, Ooi Singapore ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryoprotection possible?
Usually the cryoprotection is carried out after the specimens are fixed and before they are frozen. If you have a sample you can spare you might try making up some 20% Sucrose placing the frozen sample in it. putting it in the refrigerator and letting it stay in the 20% sucrose until it drops to the bottom of the container. You might have to change the 20% Sucrose a couple of times as you know sucrose can grow bacteria easily. When the specimen has dropped to the bottom of the container, remove it, rinse in a couple of changes of distilled water and quick freeze the sample. Try cutting and staining the sample and see if the morphology is good if it is then you can do all of your samples to correct the problem. There should be no thawing between removal of the sample from the storage to the cryostat. Hope this helps Frances Swain From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dr. med. Frauke Neff [ne...@staff.uni-marburg.de] Sent: Wednesday, March 18, 2009 3:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryoprotection possible? Dear Histonetters, I'm supposed to do a noradrenaline ihc on rat brains that have been snap frozen and afterwards glutaraldehyd fixed. While the morphology is okay in the test-tissue we used to establish the protocol, it was very poor in the tissue of the trial animals. It appeared mooth eaten and disrupted. I assume the brains were thawed and refrosted due to moving the samples between several cities. Does anyone know a method /a tip how I can save some proper morphology? I checked the archive and found sucrose as cryoprotectant, but this is supposed to do it while the tissue is frozen or is it possible to use it while the tissue thaws? Thank you all in advance for your patience and help, Frauke -- Dr. med. Frauke Neff AG Neurologische Therapieforschung Neurologie Philipps-Universität Marburg Rudolph-Bultmann Str. 8 35039 Marburg 06421/5866304 This message was sent using IMP, the Internet Messaging Program. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryoprotection possible?
You are so correct, I forgot you do not rinse the sucrose out you blot it and then freeze. Sorry about that. I do not cut frozen sections very often and I forget. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfranc...@uams.edu email -Original Message- From: anh2...@med.cornell.edu [mailto:anh2...@med.cornell.edu] Sent: Wednesday, March 18, 2009 8:41 AM To: Swain, Frances L; Dr. med. Frauke Neff; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] cryoprotection possible? Good advice indeed. However, I don't recommend you rinse in water after sucrose. Sort of defeats the purpose. Instead if you need to remove excess sucrose rinse in either 50/50 sucrose/OCT or just OCT. Also, glutaraldehyde fixation usually renders tissue difficult to immunostain. You have to consider whether your fixation is appropriate for your antigen/antibody. -Original Message- From: Swain, Frances L swainfranc...@uams.edu Date: Wed, 18 Mar 2009 08:12:45 To: Dr. med. Frauke Neffne...@staff.uni-marburg.de; histonet@lists.utsouthwestern.eduhistonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryoprotection possible? Usually the cryoprotection is carried out after the specimens are fixed and before they are frozen. If you have a sample you can spare you might try making up some 20% Sucrose placing the frozen sample in it. putting it in the refrigerator and letting it stay in the 20% sucrose until it drops to the bottom of the container. You might have to change the 20% Sucrose a couple of times as you know sucrose can grow bacteria easily. When the specimen has dropped to the bottom of the container, remove it, rinse in a couple of changes of distilled water and quick freeze the sample. Try cutting and staining the sample and see if the morphology is good if it is then you can do all of your samples to correct the problem. There should be no thawing between removal of the sample from the storage to the cryostat. Hope this helps Frances Swain From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dr. med. Frauke Neff [ne...@staff.uni-marburg.de] Sent: Wednesday, March 18, 2009 3:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryoprotection possible? Dear Histonetters, I'm supposed to do a noradrenaline ihc on rat brains that have been snap frozen and afterwards glutaraldehyd fixed. While the morphology is okay in the test-tissue we used to establish the protocol, it was very poor in the tissue of the trial animals. It appeared mooth eaten and disrupted. I assume the brains were thawed and refrosted due to moving the samples between several cities. Does anyone know a method /a tip how I can save some proper morphology? I checked the archive and found sucrose as cryoprotectant, but this is supposed to do it while the tissue is frozen or is it possible to use it while the tissue thaws? Thank you all in advance for your patience and help, Frauke -- Dr. med. Frauke Neff AG Neurologische Therapieforschung Neurologie Philipps-Universität Marburg Rudolph-Bultmann Str. 8 35039 Marburg 06421/5866304 This message was sent using IMP, the Internet Messaging Program. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cutting fresh-frozen brains from 1 week old rat pups
Definitely do at least that one sucrose cryopreservation step you mentioned. Even step up to it in 10% and 20% sucrose. --On Wednesday, March 18, 2009 4:04 PM +1100 Adam Galle adam.ga...@student.unsw.edu.au wrote: Hi all, Currently I am working with brains from 7 day old rat pups, that undergo an hypoxic-ischemic injury (Levine/Vannucci technique). These brains are unfixed, frozen in isopentane and cut at 20um on a croystat. These brains are not cutting very well compared to an adult brain (potenially due to unfinished myelination?), they are very 'crumbly' for want of a better term and always have cracks or generally poor preservation of morphology. I have tried all the standard tricks of different temperatures, section thickness and knife angle to no avail. I am going to perfuse fix my next cohort of animals with PFA and then a 30% sucrose step to see if that helps, but I was hoping that someone out there would have some tips on cutting these immature brains. Thanks, Adam. Adam Galle, Neuropharmacology and Brain Injury Lab Department of Pharmacology School of Medical Sciences UNSW Sydney ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
Ooi, As you know, working with MMA is tricky enough to have to worry about losing sections during staining. Unfortunately, I cannot speak with experience in using Bond-rite slides, but maybe there is someone monitoring this message thread that can provide helpful information on that topic. However, I can speak to the experience of using Haupts coated slides. First, it is not uncommon to observe hematoxylin background staining from Haupts coated slides. Second, you are correct in that it takes just the right concentration of Haupts solution in order to retain section adherence to the slide. Given these two observations, allow me to provide a few suggestions for working with Haupts coated slides. Before I prepare my slides, it is necessary to prepare a working dilution of Haupts solution. Whether or not you use in house prepared or commercially prepared concentrate, you should typically look to a ratio of 1:1 with liquified concentrate to 50% EtOH. This is a pretty standard dilution that works for me. You definitely do not want to go higher than a 50% concentration of Haupts, but you could try tweaking it a little within the 40-50% range. I would suggest that maybe you do a short experiment using different dilutions of the Haupts concentrate so that you may zero in on that one dilution that will minimize background staining and optimize section adherence. You may not have perfection with the hematoxylin background or aniline blue in a trichrome stain (I usually substitue aniline blue with SF light green yellowish), but you may find something that you can live with for the HE. Next, you might then try to work with the hematoxylin staining a bit. Specifically, maybe you can tweak the hematoxylin staining time by keeping it standard (I use hematoxylin 2 from Richard Allen and typically stain for 3 minutes) and/or increasing it a bit so that you can extend the acid clarifier step (I clarify or decolorize for 20 seconds) a little longer to help wash out or decolorize the background staining. Obviously you do not want to compromise your nuclear detail, but this should help a bit. Please feel free to ask any additional questions as needed. Jack Ratliff Date: Wed, 18 Mar 2009 05:33:01 -0700 From: ooitingh...@yahoo.com To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi, I just began to work with the methy methacrylate embedding plastic section. I am facing the difficulities to attach the sample on the glass slide. I have tried using Haupts adhesive coated slides and also the bond-rite slide to attach my 5micron sample. I am doing normal hematoxylin and eosin staining. The section always detach from the glass slide towards the end of the staining. I need to use the concentrated Haupts adhesive coated slide to reduce the chance for the section from lift up from the glass slide. However, I observed the high and dirty background after the staining. I noticed that some users are using Bond-rite slides to attach their samples. I tried this. However, the sections always lifted up towards the end of the staining. I am not too sure whether is my technique that giving me this kind of problem. I prewarmed the Bond-rite slides and put my sections in the 55C waterbath. Then I fished the section using the Bond-rite slide and lay it down on 55C heater. And finally in the oven incubator for more than 18 hours at 55C before staining... I would appreciate if there is any suggestion that can help to attach the section on the glass slide. Thank you very much! Yours sincerely, Ooi Singapore ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryoprotection possible?
I agree with Frances and anh2006. Cryospreservation is important for brains especially and must be done after fixation, before freezing, and the glutaraldeyde can irreversibly mask antigens. Merced --On Wednesday, March 18, 2009 8:49 AM -0500 Swain, Frances L swainfranc...@uams.edu wrote: You are so correct, I forgot you do not rinse the sucrose out you blot it and then freeze. Sorry about that. I do not cut frozen sections very often and I forget. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfranc...@uams.edu email -Original Message- From: anh2...@med.cornell.edu [mailto:anh2...@med.cornell.edu] Sent: Wednesday, March 18, 2009 8:41 AM To: Swain, Frances L; Dr. med. Frauke Neff; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] cryoprotection possible? Good advice indeed. However, I don't recommend you rinse in water after sucrose. Sort of defeats the purpose. Instead if you need to remove excess sucrose rinse in either 50/50 sucrose/OCT or just OCT. Also, glutaraldehyde fixation usually renders tissue difficult to immunostain. You have to consider whether your fixation is appropriate for your antigen/antibody. -Original Message- From: Swain, Frances L swainfranc...@uams.edu Date: Wed, 18 Mar 2009 08:12:45 To: Dr. med. Frauke Neffne...@staff.uni-marburg.de; histonet@lists.utsouthwestern.eduhistonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryoprotection possible? Usually the cryoprotection is carried out after the specimens are fixed and before they are frozen. If you have a sample you can spare you might try making up some 20% Sucrose placing the frozen sample in it. putting it in the refrigerator and letting it stay in the 20% sucrose until it drops to the bottom of the container. You might have to change the 20% Sucrose a couple of times as you know sucrose can grow bacteria easily. When the specimen has dropped to the bottom of the container, remove it, rinse in a couple of changes of distilled water and quick freeze the sample. Try cutting and staining the sample and see if the morphology is good if it is then you can do all of your samples to correct the problem. There should be no thawing between removal of the sample from the storage to the cryostat. Hope this helps Frances Swain From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dr. med. Frauke Neff [ne...@staff.uni-marburg.de] Sent: Wednesday, March 18, 2009 3:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryoprotection possible? Dear Histonetters, I'm supposed to do a noradrenaline ihc on rat brains that have been snap frozen and afterwards glutaraldehyd fixed. While the morphology is okay in the test-tissue we used to establish the protocol, it was very poor in the tissue of the trial animals. It appeared mooth eaten and disrupted. I assume the brains were thawed and refrosted due to moving the samples between several cities. Does anyone know a method /a tip how I can save some proper morphology? I checked the archive and found sucrose as cryoprotectant, but this is supposed to do it while the tissue is frozen or is it possible to use it while the tissue thaws? Thank you all in advance for your patience and help, Frauke -- Dr. med. Frauke Neff AG Neurologische Therapieforschung Neurologie Philipps-Universität Marburg Rudolph-Bultmann Str. 8 35039 Marburg 06421/5866304 This message was sent using IMP, the Internet Messaging Program. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list
Re: [Histonet] cryoprotection possible?
We tried nearly everything for the noradreanline IHC and did't get any staining. After several calls with the techs of the company, they told me I've to do a glutaraldehyd fixation, every other fixation will wash the noradrenaline out of the tissue (even the cryosection did't give a stain). Using adrenal glands as positive control, I got a weak positive stain using the GA-Fixation. But I'm not happy with this, because the samples I got are FFPE or snap frozen. So what to do to proceed? Because I don't know any way back through the formalin fixation, I decided to go on with the snap frozen tissue. I would be very happy, if someone would tell me: Hey, thats all bullshit, you just need the antibody XXX of the company YYY and verything will work fine with this noradrenaline IHC Until this happens, I try with the snap-frozen material, keep my fingers cross, throw pennies in every fontaine, rub oil-lamps and lion noses You see, I'm desperately needing help, Frauke Zitat von anh2...@med.cornell.edu: Good advice indeed. However, I don't recommend you rinse in water after sucrose. Sort of defeats the purpose. Instead if you need to remove excess sucrose rinse in either 50/50 sucrose/OCT or just OCT. Also, glutaraldehyde fixation usually renders tissue difficult to immunostain. You have to consider whether your fixation is appropriate for your antigen/antibody. -Original Message- From: Swain, Frances L swainfranc...@uams.edu Date: Wed, 18 Mar 2009 08:12:45 To: Dr. med. Frauke Neffne...@staff.uni-marburg.de; histonet@lists.utsouthwestern.eduhistonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryoprotection possible? Usually the cryoprotection is carried out after the specimens are fixed and before they are frozen. If you have a sample you can spare you might try making up some 20% Sucrose placing the frozen sample in it. putting it in the refrigerator and letting it stay in the 20% sucrose until it drops to the bottom of the container. You might have to change the 20% Sucrose a couple of times as you know sucrose can grow bacteria easily. When the specimen has dropped to the bottom of the container, remove it, rinse in a couple of changes of distilled water and quick freeze the sample. Try cutting and staining the sample and see if the morphology is good if it is then you can do all of your samples to correct the problem. There should be no thawing between removal of the sample from the storage to the cryostat. Hope this helps Frances Swain From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dr. med. Frauke Neff [ne...@staff.uni-marburg.de] Sent: Wednesday, March 18, 2009 3:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryoprotection possible? Dear Histonetters, I'm supposed to do a noradrenaline ihc on rat brains that have been snap frozen and afterwards glutaraldehyd fixed. While the morphology is okay in the test-tissue we used to establish the protocol, it was very poor in the tissue of the trial animals. It appeared mooth eaten and disrupted. I assume the brains were thawed and refrosted due to moving the samples between several cities. Does anyone know a method /a tip how I can save some proper morphology? I checked the archive and found sucrose as cryoprotectant, but this is supposed to do it while the tissue is frozen or is it possible to use it while the tissue thaws? Thank you all in advance for your patience and help, Frauke -- Dr. med. Frauke Neff AG Neurologische Therapieforschung Neurologie Philipps-Universität Marburg Rudolph-Bultmann Str. 8 35039 Marburg 06421/5866304 This message was sent using IMP, the Internet Messaging Program. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: CD86 - mouse
Hi histonetters Is really nobody working with cd86 on mouse tissues? Joost Bruijntjes TNO Quality of Life Zeist Holland TNO.NL http://www.tno.nl/ Joost Bruijntjes T +31 30 694 44 80 F +31 30 694 49 86 E joost.bruijnt...@tno.nl mailto:joost.bruijnt...@tno.nl Disclaimer http://www.tno.nl/tno/email/ This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: CLIA vs CAP grossing
CAP has deemed status from CLIA, so the CAP definition should be accepted. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax Message: 3 Date: Tue, 17 Mar 2009 11:16:22 -0600 From: rick.garnh...@memorialhealthsystem.com Subject: [Histonet] Grossing CAP is OK with their guidelines ( BUT is CLIA on line with the Processing (Low Complexity?) vs Grossing (High Complexity?). If CLIA is not on board with processing (dumb naming) being low complexity and grossing being higher complexity your lab may be in violation of CLIA Guidelines. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnh...@memorialhealthsystem.com - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mesothelioma control tissue
Hi Histonetter's, I am in need of a mesothelioma control block for my IHC. Is there anyone that would be willing to share? I might have tissue that you may need as well. Let me know. Thanks, Cheri Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Snap freezing muscle tissue for metabolic testing
Fellow histonetters: The ordering physicians/neurologists are saying that the metabolic testing is not accurate because of the way the muscle tissue is being handled currently. Does anyone have specific references that would be helpful in the argument for implementing snap freezing muscle tissue in the OR verses the reference lab when the reference lab is less than 30 minutes from the OR? Thanks in advance, Annette Foshey, HT (ASCP) Charge person in Histology Children's Hospital of Wisconsin 414-266-6580Fax 414-266-2779 afos...@chw.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] equipment purchasing
Hello, Our histo lab is currently looking into buying one or all of the following: cassette labeler, automated stainer (that can also accommodate a few special stains), and automated coverslipper (that uses minimal xylene or no xylene). Can anyone recommend good brands and models that they like as well as what vendors might have good prices? We are also looking into getting the stainer and coverslipper either used or refurbished to cut down on costs. Our lab is small and we do only about 40-50 slides per day on average. We would prefer to have the equipment to be as small as possible. Any suggestions are welcome! Thanks, Patty ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraffin Block triming
Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Paraffin Block trimming
We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald Scott lsc...@sfcn.org Sent by: histonet-boun...@lists.utsouthwestern.edu 03/18/2009 09:24 PM To Histonet@lists.utsouthwestern.edu cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
