Re: [Histonet] Looking for a CD20 that works on mouse tissue
ABCAM we r using ab9475 2009-03-25 TF 发件人： Colleen Forster 发送时间： 2009-03-25 05:27:58 收件人： Histonet 抄送： 主题： [Histonet] Looking for a CD20 that works on mouse tissue Hello Histonetters, Have a new project that wants to stain CD20 in mouse samples. One is brain and the other heart. Has anyone done this CD marker in mouse? if so, would you be willing to share with us! Thanks in advance, Colleen Forster U of MN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] QIHC certificate
Thank you all for answering this question. I really appreciate. Have a nice day, Ann Fu On Tue Mar 24 20:47:43 EDT 2009, Lee Peggy Wenk lpw...@sbcglobal.net wrote: From the ASCP Board of Registry webpage: www.ascp.org/bor Click on Qualifications Click on IHC There are 3 routes. To summarize, all experience must be within the last 5 years, in a US lab, Canadian lab, or CAP/JC accredited lab: 1. ASCP certified technologist + 6 months full time IHC experience 2. ASCP certified technian + 12 months full time IHC experience 3. BA/BS degree or higher from a regionally accredited college/university + 18 months full time IHC experience Full time acceptable experience must include: - Immunohistochemical and/or Immunofluorescence Preparations All of the following should have been performed by the applicant:- Staining technique- Selection of proper control material- Titration of immunologic reagents - Quality Assurance The applicant should have participated in Quality Assurance related to all of the following:- Specimen fixation, processing, microtomy- Reagent selection, preparation, storage, disposal - Method selection, validation, documentation- Quality control- Safety Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of FU,DONGTAO Sent: Tuesday, March 24, 2009 4:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QIHC certificate Hi, all I have a question about how to QIHC certificate. We have several bilogical scientists work as full-time employee doing IHC staining. But none of them has HT certificate. In this case, can they get QIHC certificate? What kind of courses need they to take? Thank you, Ann Fu Lab Manager Dept. of Pathology University of Flodrida Gainesville, FL 32610 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histos-5
Anyone out there using the Histos-5 from Milestone? How much reagent does a Histomodule hold? I'm trying to figure out my return on investment using reagent savings. Do you really change your paraffin less often or is that just a sales pitch? How often do you change your paraffin and what is the volume of the paraffin tank? Thanks for your help once again, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] questions re: fixing in general and Histochoice in particular
Jacqui Detmar wrote: Hi all. Having a bit of an internal debate here, so I would like to get the opinions of some of you in Histoland, please. Here are the questions: 1.When fixing with 10% NBF, for how long should you fix and what volume ratio of fixative:tissue should you use? 48 hours is a minimum according to R.D. Lillie, one week is better. Really! Thickness of the tissue does not matter, formalin fixes slowly. Of course, if you are doing immuno you may need to sacrifice some fixation to retain antigenicity. Depends on the antigen. 10X the volume of the tissue. 2. At what temperature should one be fixing tissues? Room temperature. There is more than ample scientific evidence that fixing cold just slows down the already slow fixation process. Regarding Histochoice: I never use proprietary fixes, you don't know what is in them and the manufacturer can change the formulation any time he/she wants. 1.For how long should you fix the tissue? 2. What volume ratio of fixative:tissue should you use? 3. How long can you store Histochoice-fixed tissues in 70% ethanol? I think that's about it. Thanks in advance, Jacqui Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute Mount Sinai Hospital 25 Orde Street, room 6-1001AJ Toronto, ON M5T 3H7 Email:det...@lunenfeld.ca Phone: 416-586-4800 x5607 Fax:416-586-5993 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Geoff -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Information Systems: Specimen Tracking MiddleWare
Good morning, I was just at the Lab Infotech Summit in Las Vegas last week where the subject of the conference was informatics in Anatomic and Clinical Pathology. Along with the usual seminars were the usual vendors in the exhibitor's hall demonstrating and talking about their products and services. As one of those vendors, I had the opportunity to talk to a few people and a general trend appeared to merge -- one which I would like to dispel, if possible. I'd like to make sure that everyone is aware that you do NOT have to have middleware in order to have bar coded cassettes, slides, etc., and you do NOT have to have middleware in order to have specimen/material tracking. Let me explain. If, on the one hand, you are quite content with your current information system and you simply wish to add barcodes and specimen tracking and you do not want to work with your information system vendor because either they don't have this capability or for some other reason, then YES, middleware is a viable alternative. On the other hand, if you are planning to purchase a new Information System for your laboratory, then by all means, DEMAND of your new vendor, the ability to have barcoded everything and to have specimen tracking built into your new information system. There are lots of good reasons to have all this capability in your information system and not in some middleware product. I'd be happy to discuss the reasons for my statements, but I've taken up enough of everyone's time. If you'd like to hear more, then please, just ask. I just thought everyone should know... Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Current books for Histotechnology
-Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Friday, March 20, 2009 4:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Current books for Histotechnology Our library has funds available to purchase books for the Histotechnology program. The problem is that we need current books. We have the latest Bancroft and Gamble. Any other suggestions for books that are newer than 2000? I have suggested John Kiernan's latest. By the way I did find a copy of Sheehan for $2,400!! Jennifer MacDonald Education Coordinator, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdon...@mtsac.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Part Time Histotechnologist (Chicago, IL)
Good morning! I currently have a part time Histotechnologist position available in the south suburbs of Chicago. The shifts are 10 hour days but only 1-3 days per week are required with occasional Saturdays. ASCP certification is required. Please contact me directly at jcraw...@aerotek.com if you or someone you know is interested! Best regards, Jen Jen Crawford, CIR Scientific Recruiter Aerotek Scientific Staffing Phone: 847.221.1358 Fax: 847.303.2370 www.aerotek.com http://www.aerotek.com Please do not keep me a secret...a referral is the best compliment that I can receive! This electronic mail (including any attachments) may contain information that is privileged, confidential, and/or otherwise protected from disclosure to anyone other than its intended recipient(s). Any dissemination or use of this electronic email or its contents (including any attachments) by persons other than the intended recipient(s) is strictly prohibited. If you have received this message in error, please notify us immediately by reply email so that we may correct our internal records. Please then delete the original message (including any attachments) in its entirety. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Current books for Histotechnology
Definitely get the 4th edition of Kiernan. Also get the latest edition of Polak and van Noorden's INTRODUCTION TO IMMUNOCYTOCHEMISTRY. If you don't already have them, used second or third editions of Lillie's HISTOPATHOLOGIC TECHNIC AND PRACTICAL HISTOCHEMISTRY and Pierce's HISTOCHEMISTRY, THEORETICAL AND APPLIED are surprisingly useful. -Allen A. Smith,Ph.D. Barry University School of Podiatric Medicine Miami Shores, Florida -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Friday, March 20, 2009 4:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Current books for Histotechnology Our library has funds available to purchase books for the Histotechnology program. The problem is that we need current books. We have the latest Bancroft and Gamble. Any other suggestions for books that are newer than 2000? I have suggested John Kiernan's latest. By the way I did find a copy of Sheehan for $2,400!! Jennifer MacDonald Education Coordinator, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdon...@mtsac.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Current books for Histotechnology
For an atlas, Ross and Pawlina's HISTOLOGY, Wheater's FUNCTIONAL HISTOLOGY, or Gartner and Hiatt's COLOR ATLAS OF HISTOLOGY are all good. They are also more accurate than DiFiore. -Allen a. Smith,Ph.D. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, March 23, 2009 11:55 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Current books for Histotechnology I would strongly recommend di Fiore's Atlas of Histology by Victor P. Eroschenko. I don't know what edition its in now. I used it when I went through my program. It is great for microscopic anatomy, especially when combined with actual slide viewing. Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Jennifer MacDonald Sent: Fri 3/20/2009 3:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Current books for Histotechnology Our library has funds available to purchase books for the Histotechnology program. The problem is that we need current books. We have the latest Bancroft and Gamble. Any other suggestions for books that are newer than 2000? I have suggested John Kiernan's latest. By the way I did find a copy of Sheehan for $2,400!! Jennifer MacDonald Education Coordinator, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdon...@mtsac.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] formaldehyde neutralizers
Thanks, Tony Henwood! Your explanation of how to neutralize formaldehyde with ammonia is the only clear explanation of formaldehyde neutralization I've ever read. One more question: how does neutralization with sodium bisulfite work? What are the advantages and disadvantages of the two methods? Bob Richmond Samurai Pathologist Knoxville, Tennessee ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] formaldehyde neutralizers
Me too! René J. --- On Wed, 3/25/09, Robert Richmond rsrichm...@aol.com wrote: From: Robert Richmond rsrichm...@aol.com Subject: [Histonet] formaldehyde neutralizers To: histonet@lists.utsouthwestern.edu Date: Wednesday, March 25, 2009, 10:06 AM Thanks, Tony Henwood! Your explanation of how to neutralize formaldehyde with ammonia is the only clear explanation of formaldehyde neutralization I've ever read. One more question: how does neutralization with sodium bisulfite work? What are the advantages and disadvantages of the two methods? Bob Richmond Samurai Pathologist Knoxville, Tennessee ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Supervisor, Permanent Job, FREE MEDICAL BENEFITS!
*Position of the Histology Supervisor* Do you want the benefits you deserve with the rewards you can see? Do you want the advancement, flexibility and resources to advance your career and provide the care that your patients need? **FREE medical and dental benefits, domestic partner benefits, excellent tuition reimbursement, continuing education and more!** *Description:* Supervise, coordinate and participate in providing laboratory services to meet the needs of patients as ordered by the medical staff and performed in accordance with defined standards and practices in general and unique to assigned section(s). *Day shift with variable start times - Monday - Friday* Bachelor of Science degree in Medical Technology or related biological science Six years of medical lab experience of which two years must be in particular section supervised. NYS licensure required HT, (ASCP) preferred *Montgomery**, **NY** area:* *60 miles from Danbury, CT* *60 miles from Newark, NJ* *60 miles from Scranton, PA* What a great opportunity! REMEMBER, THIS WON’T LAST. Interested? Please send resume in Microsoft Word format to: aly...@alliedsearchpartners.com **Please forward this email to anyone who you seems fit for this position, as the referral bonus for this position is $1000 if we place a person that you send to us in a position!** -- Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Frozen section
We need to make a change in the way we presently account for our frozen section while doing them. Presently we receive the requisition with the first specimen, then pathology is responsible to account for any subsequent specimens. The problem is that the subsequent specimens are typically labeled poorly, and we are trying very hard to conform to the CAP guidelines. So ... when the specimens are not labeled in detail, it requires follow up calls to gain the proper information. I would like to know how other facilities are handling multiple frozen sections. Thanks Gary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Frozen section
We require a form for each specimen. Yes, the subsequent forms may have less information (i.e. procedure being performed), but all have the patient id information, sample type and description, etc. They are treated as separate samples. -Angela Angela McNabola, MS,HT(ASCP)SLS, QIHC Manager Histology/Cytology Department of Pathology Bridgeport Hospital 267 Grant Street Bridgeport, CT 06610 phone: 203-384-4434 lam...@bpthosp.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Gary Sent: Wednesday, March 25, 2009 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen section We need to make a change in the way we presently account for our frozen section while doing them. Presently we receive the requisition with the first specimen, then pathology is responsible to account for any subsequent specimens. The problem is that the subsequent specimens are typically labeled poorly, and we are trying very hard to conform to the CAP guidelines. So ... when the specimens are not labeled in detail, it requires follow up calls to gain the proper information. I would like to know how other facilities are handling multiple frozen sections. Thanks Gary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Current books for Histotechnology
A for atlases, I also like Wheater's FUNCTIONAL HISTOLOGY, or Gartner and Hiatt's COLOR ATLAS OF HISTOLOGY. I don't like Ross and Pawlina's HISTOLOGY, the third edition by Ross, Romrell and Kaye is much better and might be cheaper. Geoff Smith, Allen wrote: For an atlas, Ross and Pawlina's HISTOLOGY, Wheater's FUNCTIONAL HISTOLOGY, or Gartner and Hiatt's COLOR ATLAS OF HISTOLOGY are all good. They are also more accurate than DiFiore. -Allen a. Smith,Ph.D. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, March 23, 2009 11:55 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Current books for Histotechnology I would strongly recommend di Fiore's Atlas of Histology by Victor P. Eroschenko. I don't know what edition its in now. I used it when I went through my program. It is great for microscopic anatomy, especially when combined with actual slide viewing. Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Jennifer MacDonald Sent: Fri 3/20/2009 3:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Current books for Histotechnology Our library has funds available to purchase books for the Histotechnology program. The problem is that we need current books. We have the latest Bancroft and Gamble. Any other suggestions for books that are newer than 2000? I have suggested John Kiernan's latest. By the way I did find a copy of Sheehan for $2,400!! Jennifer MacDonald Education Coordinator, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdon...@mtsac.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RNA and DNA yields from Laser Microdissection
Histonet: I have a couple questions I would like to throw out to the experts: 1. Do you think higher yields of DNA /RNA in LMD are related to protocol, method differences from one instrument to another ( i.e. catapult verses,, gravity drop, verses, arcturus melt system...or more related to histologist v. research tech handling of the sample? 2. How do you feel about frozen verses paraffin as related to yield and quality in LMD/LCM? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Frozen section
Somewhere in the USA Gary Martin asks: We need to make a change in the way we presently account for our frozen section [specimens] while doing [the frozen sections]. Presently we receive the requisition with the first specimen, then pathology is responsible to account for any subsequent specimens. The problem is that the subsequent specimens are typically labeled poorly, and we are trying very hard to conform to the CAP guidelines. So ... when the specimens are not labeled in detail, it requires follow up calls to gain the proper information. I would like to know how other facilities are handling multiple frozen sections. Well, somewhere in the frozen section process the pathologist, who presumably knows what the specimen is, has to record a diagnosis on paper. That diagnosis should certainly include the anatomic site (which I suppose is your problem). Thus I might write: 3. skin of nose (re-excision of 3 to 6 o'clock margin): no basal cell carcinoma seen. I think you need to ask your pathologists to help you with this problem. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] re: water pH
I don't know that there's a way to change the pH of water through filtration. The pH is affected mostly by dissolved gasses and ions which aren't easily removed through filtration alone. You can adjust the pH by using buffered solutions, but then its really not water anymore. If you just have bad tap water, I am not sure the cost of RO but I am fairly sure its drastically cheaper than a large-scale distilltion setup and might give you water of sufficient quality. I would say the absolute best solution for good H20 is distillation. It doesn't have to be an incredibly fancy setup, and would depend largely on your demand. You may even have luck locating some used equipment for the job in the case that you need to generate lots of it. And if your lab is broke most of the time like mine, for small quantities, you could just use a simple DIY setup. The still has been around for centuries and really hasn't changed drastically in terms of design and basic function. Either way I think it would be worth the investment. -Matt ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Opinions on the Labvision 360 autostainer?
Hi everyone, Has anyone used the Labvision 360 autostainer? Any good or bad things about it? Is it suitable for IHC on animal tissues? Trying to sort out which stainer will work best for us and which is the most economical of course. I appreciate any feedback. Please no inquiries from sales reps. Thanks Jen C. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Plastic Thin Sections Won't Stay
We are re-visiting thin sectioning of MMA embedded items. The sections are great, but none of them stay on the slides for the staining. We're trying Sta-On, and have tried chrome alum slides. We use a butoxy mixture to stretch them and immuno quality slides. Any ideas? Thanks for your help! -- Corliss Harris Histology Technician MED Institute 1 Geddes Way West Lafayette, IN 47906 765-463-7537 ext. 1150 765-497-0641-fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Frozen section
Gary, We require an additional requisition with additional specimens labeled and numbered correctly in sequential order. In other words, if a second and third specimen is sent, the 2nd requisition will mark the #1 specimen as Previously sent for FS and the additional specimens will be listed in the #2, #3, spot on the requisition. Does that make sense? Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Gary Sent: Wednesday, March 25, 2009 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen section We need to make a change in the way we presently account for our frozen section while doing them. Presently we receive the requisition with the first specimen, then pathology is responsible to account for any subsequent specimens. The problem is that the subsequent specimens are typically labeled poorly, and we are trying very hard to conform to the CAP guidelines. So ... when the specimens are not labeled in detail, it requires follow up calls to gain the proper information. I would like to know how other facilities are handling multiple frozen sections. Thanks Gary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE:guide to special stains
http://www.dako.com/prod_productrelatedinformation?url=literature_guides.htm You can also email them and request the guide. Or you can download the PDF, but it is VERY long, too much to print out. Does anyone have or know where to obtain a list of special stains that briefly describes which each one is for? Thanks, Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 -- Does anyone have or know where to obtain a list of special stains that briefly describes which each one is for? Thanks, Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 -- Does anyone have or know where to obtain a list of special stains that briefly describes which each one is for? Thanks, Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 -- ___ Suzanne Bruce, R.V.T. Histologist Necropsy Coordinator Vet Path Services, Inc. (VPS) 6450 Castle Dr. Mason, OH 45040 Lab: (513) 469-0777 Fax: (513) 469-2474 Email: sbr...@vetpathservicesinc.com www.vetpathservicesinc.com http://www.vetpathservicesinc.com/ https://69.61.197.115/exchweb/bin/redir.asp?URL=http://www.vetpathservicesinc.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Collagen 111
I was wondering if anyone has done Collagen 111 antibody IHC on FFPE mouse tissue?(Bone) If so, and you got good results, could you please let me know where the Antibody is from, and also the protocol you used. High heat for antigen retreival is not working well for the bone tissue , and I have got weak results trying Hyaluronidase, Prot. K, and Trypsin. Any help would be appreciated. Thanks, Nancy Lowen clayca...@yahoo.net Research ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 64, Issue 44
4-12 hours dependent on sample size (a nickel if a good reference)...volume 1:20 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, March 25, 2009 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 64, Issue 44 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Re: questions re: fixing in general and Histochoice in particular (Geoff McAuliffe) 2. Information Systems: Specimen Tracking MiddleWare (Michael Mihalik) 3. RE: Current books for Histotechnology (Smith, Allen) 4. Part Time Histotechnologist (Chicago, IL) (Crawford, Jennifer) 5. RE: Current books for Histotechnology (Smith, Allen) 6. RE: Current books for Histotechnology (Smith, Allen) 7. formaldehyde neutralizers (Robert Richmond) 8. Re: formaldehyde neutralizers (Rene J Buesa) 9. Histology Supervisor, Permanent Job, FREE MEDICAL BENEFITS! (Alyssa Peterson) 10. Frozen section (Martin, Gary) 11. RE: Frozen section (McNabola, Angela) 12. Re: Current books for Histotechnology (Geoff McAuliffe) 13. RE: Frozen section (Weems, Joyce) 14. RNA and DNA yields from Laser Microdissection (Barone, Carol ) 15. Re: RNA and DNA yields from Laser Microdissection (ne...@staff.uni-marburg.de) -- Message: 1 Date: Wed, 25 Mar 2009 09:35:32 -0400 From: Geoff McAuliffe mcaul...@umdnj.edu Subject: Re: [Histonet] questions re: fixing in general and Histochoice in particular To: Jacqui Detmar det...@lunenfeld.ca Cc: histonet@lists.utsouthwestern.edu Message-ID: 49ca3324.1070...@umdnj.edu Content-Type: text/plain; charset=ISO-8859-1; format=flowed Jacqui Detmar wrote: Hi all. Having a bit of an internal debate here, so I would like to get the opinions of some of you in Histoland, please. Here are the questions: 1.When fixing with 10% NBF, for how long should you fix and what volume ratio of fixative:tissue should you use? 48 hours is a minimum according to R.D. Lillie, one week is better. Really! Thickness of the tissue does not matter, formalin fixes slowly. Of course, if you are doing immuno you may need to sacrifice some fixation to retain antigenicity. Depends on the antigen. 10X the volume of the tissue. 2. At what temperature should one be fixing tissues? Room temperature. There is more than ample scientific evidence that fixing cold just slows down the already slow fixation process. Regarding Histochoice: I never use proprietary fixes, you don't know what is in them and the manufacturer can change the formulation any time he/she wants. 1.For how long should you fix the tissue? 2. What volume ratio of fixative:tissue should you use? 3. How long can you store Histochoice-fixed tissues in 70% ethanol? I think that's about it. Thanks in advance, Jacqui Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute Mount Sinai Hospital 25 Orde Street, room 6-1001AJ Toronto, ON M5T 3H7 Email:det...@lunenfeld.ca Phone: 416-586-4800 x5607 Fax:416-586-5993 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Geoff -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** -- Message: 2 Date: Wed, 25 Mar 2009 08:46:11 -0500 From: Michael Mihalik m...@pathview.com Subject: [Histonet] Information Systems: Specimen Tracking MiddleWare To: histonet@lists.utsouthwestern.edu Message-ID: 00c001c9ad50$13204280$3960c7...@com Content-Type: text/plain; charset=US-ASCII Good morning, I was just at the Lab Infotech Summit in Las Vegas last week where the subject of the conference was informatics in Anatomic and Clinical Pathology. Along with the usual seminars were the usual vendors in the exhibitor's hall demonstrating and talking about their products and services. As one of those vendors, I had the opportunity to talk to a few people and a general trend appeared to
RE: [Histonet] Plastic Thin Sections Won't Stay
Try coating your slides with Haupt's adhesive. It can be purchased commercially from Fisher (or you can make it yourself using glycerin, gelatin, and phenol) and then cut it 1:1 with 50% EtOH to prepare a working dilution. I have used Haupt's for many years on both small (1x3 slides) and large (2x3 slides) and have NEVER lost a section (knocking on wood now...LOL) to staining. Make sure to look over your slides before use to insure adequate coating of the slide. Please keep in mind that with every new thing you bring into the lab, you should do some testing of your own. Specifically, you will want to research the concentrate that works best for your staining as it does yield to some degree of background when staining with hematoxylin, fast green, and aniline blue if the concentration is too high. Jack Ratliff Date: Wed, 25 Mar 2009 15:58:05 -0400 From: har...@medinst.com To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plastic Thin Sections Won't Stay We are re-visiting thin sectioning of MMA embedded items. The sections are great, but none of them stay on the slides for the staining. We're trying Sta-On, and have tried chrome alum slides. We use a butoxy mixture to stretch them and immuno quality slides. Any ideas? Thanks for your help! -- Corliss Harris Histology Technician MED Institute 1 Geddes Way West Lafayette, IN 47906 765-463-7537 ext. 1150 765-497-0641-fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Frozen section
We require our OR to submit a separate requisition with each container. The specimen site is listed on each requisition. This applies to all specimens (not just frozens). In addition, frozens (and other intra operative consultations such as gross only or touch prep) must also have a frozen section form for each container. The frozen section form has patient ID, clinical history and specimen site fields completed by the OR and a diagnosis field which is completed and signed by the pathologist. Original goes to patient chart; copy stays in Pathology. When a specimen is submitted for frozen, all 3 must have matching information: container, requisition and frozen section form. If discrepant, OR must fix immediately before the frozen is signed out. Any later specimen, even on same patient, must have a separate requisition and (if applicable) a frozen form. It is the responsibility of the OR to accurately identify the specimen site. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Gary Sent: Wednesday, March 25, 2009 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen section We need to make a change in the way we presently account for our frozen section while doing them. Presently we receive the requisition with the first specimen, then pathology is responsible to account for any subsequent specimens. The problem is that the subsequent specimens are typically labeled poorly, and we are trying very hard to conform to the CAP guidelines. So ... when the specimens are not labeled in detail, it requires follow up calls to gain the proper information. I would like to know how other facilities are handling multiple frozen sections. Thanks Gary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formalin and Solvent Recyclers
I'm looking for solvent and formalin recyclers that have a fairly small footprint and fairly straight forward ventilation requirements. Any suggestions or critiques of recyclers. Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfe...@cmc-nh.org mailto:sfe...@cmc-nh.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
