[Histonet] CD25
Hi everyone I have a researcher wishing to look at CD25 in canine tissue. Has anyone any information that could help? Many thanks Neil MacIntyre ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Embeeding frozen tissue in paraffin
Hello, is there any good method for embeeding fresh frozen tissue (without any fixation) in paraffin? I would be grateful for ANY suggestions. T. Bonda ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Embeeding frozen tissue in paraffin
is there any good method for embeeding fresh frozen tissue (without any fixation) in paraffin? Wouldn't the molten paraffin would melt the frozen tissue? LOL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CD25
Google is always a good first step: http://www.google.com/#hl=enq=CD25+in+canine+tissuebtnG=Google+Searchaq=foq=CD25+in+canine+tissuefp=-dx2DTn7Pl4 On Fri, May 15, 2009 at 4:02 AM, NEIL MACINTYRE neil.macint...@btinternet.com wrote: Hi everyone I have a researcher wishing to look at CD25 in canine tissue. Has anyone any information that could help? Many thanks Neil MacIntyre ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] 70% alcohol fixation of brain
Greetings TF: Good luck freezing a brain that is full of alcohol! Have you checked the freezing point of alcohol? Why are you doing this? Immersing a whole brain in 70% alcohol, why?? 30% sucrose is a cryoprotectant so the brain is not full of holes from ice crystals.Alcohol defeats this purpose. Geoff TF wrote: Hi, i dont want to use PFA for the brain fixation (rat). Now I tried to perfuse the rat with saline, followed with 70% alcohol. Then I do post-fixation at room temperature for 24 hours. I also tried saline perfusion, then I directly put the whole brain into 70% alcohol. Is this fine? Also, for dehydration before cutting frozen sections on a cryostat/microtome, should I use 95% alcohol? I am now using 30% sucrose~ 2009-05-15 TF ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Customary fees
It seems that people are suing for any and everything they can these days I would too if I was misdiagnosed or otherwise the victim of malpractice involving incorrect/erroneous histopathology. Don't disrespect such victims by lumping them in the damn litigious people these days! crowd. On Fri, May 15, 2009 at 11:44 AM, Mike Pence mpe...@grhs.net wrote: Hi All, I am just wanting to get an idea of how many of you charge law firms or legal gathering sites for making copies of pathology reports and sending slides and blocks. It seems that people are suing for any and everything they can these days. I would also like to know what people are charging for their copying and time with these cases. Please respond off line if you don't want to publish your fees. Thanks, Mike Pence AP Supervisor GRMC West Burlington, Iowa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] 70% alcohol fixation of brain
Good luck freezing a brain that is full of alcohol! Have you checked the freezing point of alcohol? Why are you doing this? I think that moire often than not, people ask before they think ;-) On Fri, May 15, 2009 at 11:56 AM, Geoff McAuliffe mcaul...@umdnj.eduwrote: Greetings TF: Good luck freezing a brain that is full of alcohol! Have you checked the freezing point of alcohol? Why are you doing this? Immersing a whole brain in 70% alcohol, why?? 30% sucrose is a cryoprotectant so the brain is not full of holes from ice crystals.Alcohol defeats this purpose. Geoff TF wrote: Hi, i dont want to use PFA for the brain fixation (rat). Now I tried to perfuse the rat with saline, followed with 70% alcohol. Then I do post-fixation at room temperature for 24 hours. I also tried saline perfusion, then I directly put the whole brain into 70% alcohol. Is this fine? Also, for dehydration before cutting frozen sections on a cryostat/microtome, should I use 95% alcohol? I am now using 30% sucrose~ 2009-05-15 TF ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] KP Marker Plus
Does anyone know who sells the KP Marker PLUS? I can't seem to find them. Thanks for any help. Ken Kenneth G Metzger HTL(ASCP) ARUP Labs Histology Supervisor 500 Chipeta Way Salt Lake City, Utah 84108-1221 kenneth.metz...@aruplab.com (801) 583-2787 x 3101 - -- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] KP Marker Plus
Mercedes Medical 800-331-2716 Betsy Molinari HT (ASCP) Texas heart Institute Cardiovascular Pathology Houston,TX 77090 832-355-6524 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth Sent: Friday, May 15, 2009 11:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] KP Marker Plus Does anyone know who sells the KP Marker PLUS? I can't seem to find them. Thanks for any help. Ken Kenneth G Metzger HTL(ASCP) ARUP Labs Histology Supervisor 500 Chipeta Way Salt Lake City, Utah 84108-1221 kenneth.metz...@aruplab.com (801) 583-2787 x 3101 - -- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Embeeding frozen tissue in paraffin
I assume you mean the tissue was frozen without fixation, not that you want to put it into paraffin without fixation? No tissue will withstand paraffin processing without fixation. The best way to turn a frozen tissue into a paraffin embedded tissue is to drop the frozen tissue (either plain or in embedding medium, whichever is the case) into formalin while still frozen. Let it thaw and fix in the formalin, using a typical fixation time for a tissue that size, then just process and embed it as you would any tissue. -- From: histonet-boun...@lists.utsouthwestern.edu on behalf of Tomasz Bonda Sent: Friday, May 15, 2009 6:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embeeding frozen tissue in paraffin Hello, is there any good method for embeeding fresh frozen tissue (without any fixation) in paraffin? I would be grateful for ANY suggestions. T. Bonda ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] KP Marker Plus
Ken I am sorry that our contact info isnt on the pens themselves. We are the U.S. source for the Klinipath KP Pen. Thanks Dave Johnson Mercedes Medical 7590 Commerce Court Sarasota, FL 34243 800-331-2716 www.mercedesmedical.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth Sent: Friday, May 15, 2009 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] KP Marker Plus Does anyone know who sells the KP Marker PLUS? I can't seem to find them. Thanks for any help. Ken Kenneth G Metzger HTL(ASCP) ARUP Labs Histology Supervisor 500 Chipeta Way Salt Lake City, Utah 84108-1221 kenneth.metz...@aruplab.com (801) 583-2787 x 3101 - -- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] KP Marker Plus
Indeed! My box of 12 pens just arrived today. Cat# KLI KPPEN. www.mercedesmedical.com Merced --On Friday, May 15, 2009 11:16 AM -0500 Molinari, Betsy bmolin...@heart.thi.tmc.edu wrote: Mercedes Medical 800-331-2716 Betsy Molinari HT (ASCP) Texas heart Institute Cardiovascular Pathology Houston,TX 77090 832-355-6524 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth Sent: Friday, May 15, 2009 11:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] KP Marker Plus Does anyone know who sells the KP Marker PLUS? I can't seem to find them. Thanks for any help. Ken Kenneth G Metzger HTL(ASCP) ARUP Labs Histology Supervisor 500 Chipeta Way Salt Lake City, Utah 84108-1221 kenneth.metz...@aruplab.com (801) 583-2787 x 3101 - -- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 lei...@buffalo.edu 716-829-6118 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] KP Marker Plus
By the way, they are only $55/box... :-) --On Friday, May 15, 2009 1:17 PM -0400 Merced M Leiker lei...@buffalo.edu wrote: Indeed! My box of 12 pens just arrived today. Cat# KLI KPPEN. www.mercedesmedical.com Merced --On Friday, May 15, 2009 11:16 AM -0500 Molinari, Betsy bmolin...@heart.thi.tmc.edu wrote: Mercedes Medical 800-331-2716 Betsy Molinari HT (ASCP) Texas heart Institute Cardiovascular Pathology Houston,TX 77090 832-355-6524 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth Sent: Friday, May 15, 2009 11:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] KP Marker Plus Does anyone know who sells the KP Marker PLUS? I can't seem to find them. Thanks for any help. Ken Kenneth G Metzger HTL(ASCP) ARUP Labs Histology Supervisor 500 Chipeta Way Salt Lake City, Utah 84108-1221 kenneth.metz...@aruplab.com (801) 583-2787 x 3101 - -- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 lei...@buffalo.edu 716-829-6118 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 lei...@buffalo.edu 716-829-6118 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] KP Marker Plus
Does anyone know who sells the KP Marker PLUS? I can't seem to find them. Thanks for any help. Did you try Googling it? http://www.google.com/#hl=enq=KP+Marker+PlusbtnG=Google+Searchaq=foq=KP+Marker+Plusfp=-dx2DTn7Pl4 On Fri, May 15, 2009 at 12:12 PM, Metzger, Kenneth kenneth.metz...@aruplab.com wrote: Does anyone know who sells the KP Marker PLUS? I can't seem to find them. Thanks for any help. Ken Kenneth G Metzger HTL(ASCP) ARUP Labs Histology Supervisor 500 Chipeta Way Salt Lake City, Utah 84108-1221 kenneth.metz...@aruplab.com (801) 583-2787 x 3101 - -- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Embedding Frozen Tissue in Paraffin/
I agree with Paul Monfils, there needs to be fixation prior to embedding in paraffin plus the tissue needs to be infiltrated or the paraffin will not penetrate the frozen tissue. Even if you are not looking for infiltration, the hot wax will eventually harden and the wet tissue will fall out or shrink. Over long term storage the tissue will decay or grow lots of fungus and mold. Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarli...@psu.edu *E-Mail Confidentiality Notice* This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Embeeding frozen tissue in paraffin
Embedding frozen tissue in Pwax is very possible, Tomasz. If your tissue has been frozen NOT using OCT, just place the frozen tissue into acetone that has been cooled to -20C. x3 changes of -20C acetone , then immediately place tissue into molten pwax x3 changes. Agitate frequently, gently. I assume that your specimens are small: you will have more problems with morphology if you have BIG pieces of tissue. I have done this with whole mouse cerebellum and the pwax HEs looked OK...I never went further due to a job change. (I used x3 acetone changes over 24Hrs, then x3 Pwax over a period of 3Hrs) Very good Q...please keep experimenting and asking. However, it is always best to give a reason for your Q, then you might not get questions to your questions ;-) So, why do you want to do this? Carl. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HT training in Virginia
I am a MLT registry eligible technician. I received my MLT training in the military. I would like to get into histology. I am looking for an opportunity to gain experience, either through working or and internship. I have no prior histology training but I am eager to learn and I am a fast learner. I live in the Richmond area and I am willing to travel no more than 2 hours. If given an opportunity to work, this training would be my main priority. Anyway, if anyone can help me out it would be greatly appreciated. Thanks! Krista kris_...@verizon.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] QA Manual Question
Hi All...Again. I guess I have a lot of questions lately. I have asked this before but I would like to ask it again. What are people doing for QA/QC? I have been asked by management to write a QA manual. I have some ideas but not really sure where to start. Thanks in advance for any input. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
