[Histonet] Immunohistochemistry
I am working in immuno Lab doing hgh volume and i need resource where i can have some continuing education.Thanks if any one can help. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cytoseal XYL
Has anyone compared Cytoseal 60 with Cytoseal XYL? I clear with Xylene and use cytoseal 60 (Toluene)to coverslip. Seems like it would make sense to use Cytoseal XYL (Xylene) but they only sell in a case and I can't get a sample. I would be interested to know if anyone has a preference. Thanks!! Mike ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: AW: [Histonet] Chemicals that inactivate the primary antibody
Hi TF and all interested, I think I know what you want, but unfortunately I don't know how to answer your question (it is something I'd like answered myself!!) To re-word for the sake of all interested: You want to perform double-immunofluorescent staining using 2 primaries that were raised in the same species. An additional question for clarification is: Do you want to do this on paraffin or frozen sections? Maybe someone can help figure it out... Merced --On Sunday, May 17, 2009 2:16 AM +0800 TF ti...@foxmail.com wrote: But the heat also damage the fluorescnece... u need specific amplication kit anyway. 2009-05-17 TF 发件人： Gudrun Lang 发送时间： 2009-05-17 02:00:18 收件人： ti...@foxmail.com 抄送： histonet@lists.utsouthwestern.edu 主题： AW: [Histonet] Chemicals that inactivate the primary antibody For doublestaining the primary undergoes denaturation through a second HIER-step. The second secondary ab doesn't bind to the first primary. Therefore the binding sites must have been destroyed by heat in retrieval-buffer. Gudrun -Urspr黱gliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von TF Gesendet: Samstag, 16. Mai 2009 18:48 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] Chemicals that inactivate the primary antibody Hi all, just wonder what kind of treatments/chemicals can complete block the binding of 2nd antibody to binded primary antibody on antigen? I tried HCl , but does not damage all the primaryantibody binding sites - i can still see staining pattern finally. 2009-05-17 TF ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 lei...@buffalo.edu 716-829-6118 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Canine CD25
Hi everyone. I have a researcher that would like to look at CD25 in canine tissue. Has anyone ever used this antibody? Any information would be much appreciated Neil MacIntyre CSci FIBMS Laboratory Manager Veterinary Pathology Unit Easter Bush Veterinary Centre Nr Roslin Midlothian EH25 9RG phone:0131 650 6403/8802 Fax: 0131 445 5770 The University of Edinburgh is a charitable body, registered in Scotland, with registration number SC005336. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] DAB WASTE
Hello Jennifer, We are a hospital site and operate 2 Dako Autostainers. We collect our DAB waste into empty plastic carboys from Coulter Diluent used in our Hematology Department. These containers are encased within a cardboard cover. They are very convenient as we can place the hose directly into the waste container from the Dako itself. Our volume is such that we discard the containers on a weekly basis. Hope this information is useful to you Karen K Histopathology Dept Lethbridge Regional Hospital Lethbridge,Alberta, CANADA -- Message: 6 Date: Wed, 13 May 2009 05:51:33 -0700 From: Jennifer Campbell jcampb...@vdxpathology.com Subject: [Histonet] Question about DAB waste To: histonet@lists.utsouthwestern.edu Message-ID: 5658cbdb9eae6545abe50d2563d81bf821c...@vdxserver01.vdxpathology.local Content-Type: text/plain; charset=US-ASCII We just purchased a Dako Autostainer and are in the process of figuring out how to get rid of the haz waste that we will be generating. The machine separates the haz from non-haz, thankfully. I've been communicating with our waste facility and have been having some difficulty. They want to know a lot of specifics like, how much waste we will be brining to them at a time and how often, what sort of containers we will be bringing it in etc. Do you let the haz waste carboy get to a certain level before emptying it? Is it ok to pour this waste into plastic containers of some sort (we were going to use empty distilled water bottles) to transport them to the facility? I would appreciate any insight. Thank you. Jennifer C. This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] KP Marker Plus
That is Mercedes Medicals LIST Price for those accounts that order online and order 1 box per year. If you order through a Histology Sales Rep (800-331-2716) You may enjoy a discounted price. Because Mercedes Medical Reps are nice and appreciate great customers! -Original Message- From: Dave Johnson Sent: Friday, May 15, 2009 1:34 PM To: Sales Inside; Marketing Department Subject: FW: [Histonet] KP Marker Plus Theres some great free marketing. Anyone want to couthly respond? Or maybe lower our list price. We don't sell to that many at that price Whose? SUNY Buffalo , asses -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced M Leiker Sent: Friday, May 15, 2009 1:23 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] KP Marker Plus By the way, they are only $55/box... :-) --On Friday, May 15, 2009 1:17 PM -0400 Merced M Leiker lei...@buffalo.edu wrote: Indeed! My box of 12 pens just arrived today. Cat# KLI KPPEN. www.mercedesmedical.com Merced --On Friday, May 15, 2009 11:16 AM -0500 Molinari, Betsy bmolin...@heart.thi.tmc.edu wrote: Mercedes Medical 800-331-2716 Betsy Molinari HT (ASCP) Texas heart Institute Cardiovascular Pathology Houston,TX 77090 832-355-6524 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth Sent: Friday, May 15, 2009 11:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] KP Marker Plus Does anyone know who sells the KP Marker PLUS? I can't seem to find them. Thanks for any help. Ken Kenneth G Metzger HTL(ASCP) ARUP Labs Histology Supervisor 500 Chipeta Way Salt Lake City, Utah 84108-1221 kenneth.metz...@aruplab.com (801) 583-2787 x 3101 - -- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 lei...@buffalo.edu 716-829-6118 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 lei...@buffalo.edu 716-829-6118 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thermo-Fisher Products
I was just wondering what everyone's thoughts were on thermo products. I have been demo-ing an Exlcelsior Processor and was wondering if anyone had any problems. Let me know. LeAnn Murphy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD25
Hi everyone. I have a researcher that would like to look at CD25 in canine tissue. Has anyone ever used this antibody? Any information would be much appreciated Neil MacIntyre CSci FIBMS Laboratory Manager Veterinary Pathology Unit Easter Bush Veterinary Centre Nr Roslin Midlothian EH25 9RG phone:0131 650 6403/8802 Fax: 0131 445 5770 The University of Edinburgh is a charitable body, registered in Scotland, with registration number SC005336. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: AW: [Histonet] Chemicals that inactivate the primary antibody
I am completely confused. IF you have an epitope in the tissue and you react with it an specific antibody that is it. The epitope will have reacted and I do not see any way in which you can add another antibody to that initial epitope. From the reactive point of view, the epitope is blocked to react with another, unless the reaction is not totally specific and there is room for another. I just cannot imagine a way of doing this. René J. --- On Mon, 5/18/09, Merced M Leiker lei...@buffalo.edu wrote: From: Merced M Leiker lei...@buffalo.edu Subject: Re: AW: [Histonet] Chemicals that inactivate the primary antibody To: ti...@foxmail.com, gu.l...@gmx.at Cc: histonet@lists.utsouthwestern.edu Date: Monday, May 18, 2009, 9:42 AM Hi TF and all interested, I think I know what you want, but unfortunately I don't know how to answer your question (it is something I'd like answered myself!!) To re-word for the sake of all interested: You want to perform double-immunofluorescent staining using 2 primaries that were raised in the same species. An additional question for clarification is: Do you want to do this on paraffin or frozen sections? Maybe someone can help figure it out... Merced --On Sunday, May 17, 2009 2:16 AM +0800 TF ti...@foxmail.com wrote: But the heat also damage the fluorescnece... u need specific amplication kit anyway. 2009-05-17 TF 发件人： Gudrun Lang 发送时间： 2009-05-17 02:00:18 收件人： ti...@foxmail.com 抄送： histonet@lists.utsouthwestern.edu 主题： AW: [Histonet] Chemicals that inactivate the primary antibody For doublestaining the primary undergoes denaturation through a second HIER-step. The second secondary ab doesn't bind to the first primary. Therefore the binding sites must have been destroyed by heat in retrieval-buffer. Gudrun -Urspr黱gliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von TF Gesendet: Samstag, 16. Mai 2009 18:48 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] Chemicals that inactivate the primary antibody Hi all, just wonder what kind of treatments/chemicals can complete block the binding of 2nd antibody to binded primary antibody on antigen? I tried HCl , but does not damage all the primaryantibody binding sites - i can still see staining pattern finally. 2009-05-17 TF ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 lei...@buffalo.edu 716-829-6118 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Thermo-Fisher Products
We have an Excelsior that is about 5 years old. We have had a lot of issues and have had service too many times in the past year to count. We are looking at replacing it. Perhaps we got a lemon, but I don't think I could recommend it based on our experience. Are there any other processors anyone would like to recommend? Also, how about microwave processors and IHC? Thanks, Carol -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Leann M. Murphy Sent: Monday, May 11, 2009 10:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo-Fisher Products I was just wondering what everyone's thoughts were on thermo products. I have been demo-ing an Exlcelsior Processor and was wondering if anyone had any problems. Let me know. LeAnn Murphy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE OF CONFIDENTIALITY This electronic message, including attachments, is for the sole use of the named recipient and may contain confidential or privileged information protected by the State of Kentucky and/or Federal regulations. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. If you are not the intended recipient and have received this communication in error, please contact the sender and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: Re: AW: [Histonet] Chemicals that inactivate the primary antibody
Thanks all, Merced M Leiker is right: the double IHC procedure using two primary antibodies from same species. Some expert just sent me their procedure of Heat-interval based inactivation of the primary antibody for first antigen. I am just seeking for a chemical way at room temperature. 2009-05-18 TF 发件人： Rene J Buesa 发送时间： 2009-05-18 22:31:30 收件人： tifei; gu.lang; Merced M Leiker 抄送： histonet 主题： Re: AW: [Histonet] Chemicals that inactivate the primary antibody I am completely confused. IF you have an epitope in the tissue and you react with it an specific antibody that is it. The epitope will have reacted and I do not see any way in which you can add another antibody to that initial epitope. From the reactive point of view, the epitope is blocked to react with another, unless the reaction is not totally specific and there is room for another. I just cannot imagine a way of doing this. René J. --- On Mon, 5/18/09, Merced M Leiker lei...@buffalo.edu wrote: From: Merced M Leiker lei...@buffalo.edu Subject: Re: AW: [Histonet] Chemicals that inactivate the primary antibody To: ti...@foxmail.com, gu.l...@gmx.at Cc: histonet@lists.utsouthwestern.edu Date: Monday, May 18, 2009, 9:42 AM Hi TF and all interested, I think I know what you want, but unfortunately I don't know how to answer your question (it is something I'd like answered myself!!) To re-word for the sake of all interested: You want to perform double-immunofluorescent staining using 2 primaries that were raised in the same species. An additional question for clarification is: Do you want to do this on paraffin or frozen sections? Maybe someone can help figure it out... Merced --On Sunday, May 17, 2009 2:16 AM +0800 TF ti...@foxmail.com wrote: But the heat also damage the fluorescnece... u need specific amplication kit anyway. 2009-05-17 TF 发件人： Gudrun Lang 发送时间： 2009-05-17 02:00:18 收件人： ti...@foxmail.com 抄送： histonet@lists.utsouthwestern.edu 主题： AW: [Histonet] Chemicals that inactivate the primary antibody For doublestaining the primary undergoes denaturation through a second HIER-step. The second secondary ab doesn't bind to the first primary. Therefore the binding sites must have been destroyed by heat in retrieval-buffer. Gudrun -Urspr黱gliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von TF Gesendet: Samstag, 16. Mai 2009 18:48 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] Chemicals that inactivate the primary antibody Hi all, just wonder what kind of treatments/chemicals can complete block the binding of 2nd antibody to binded primary antibody on antigen? I tried HCl , but does not damage all the primaryantibody binding sites - i can still see staining pattern finally. 2009-05-17 TF ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 lei...@buffalo.edu 716-829-6118 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] control tissue
We have a plentiful amount (hundreds) of malignat melanoma blocks that we are willing to trade some for any ER/PR + tissue, colon cancer, or any other uselful IHC control tissue. Any takers? This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secured or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. NeoGenomics Laboratories, Suite 5, 12701 Commonwealth Dr, Fort Myers, FL 33913, http://www.neogenomics.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: Re: [Histonet] Chemicals that inactivate the primary antibody
TF, Two things you can do; If you are using a chromogenic system (not fluorescence) you can use DAB as the first chromogen. The DAB polymerized product will cover the first primary and prevent cross-reaction. Or, if using IF you can use unlabled Fab fragments to coat the first primary. For instance, use donkey anti-mouse to coat the first primary (if it is a mouse antibody). See Jackson Immuno Research for the antibody fragments. See this link for full information: http://www.jacksonimmuno.com/technical/fab-blok.asp Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of TF Sent: Saturday, May 16, 2009 10:38 AM To: Rene J Buesa; Histonet@lists.utsouthwestern.edu Subject: Re: Re: [Histonet] Chemicals that inactivate the primary antibody Hi, but I dont want to destory everything I am trying to see if I can prevent the binding of another 2nd antibody to the primary antibody for the first antigen! There fore you can perform double staining from same sepecies-derived primary antibodies! 2009-05-17 TF 发件人： Rene J Buesa 发送时间： 2009-05-17 00:54:26 收件人： Histonet@lists.utsouthwestern.edu; tifei 抄送： 主题： Re: [Histonet] Chemicals that inactivate the primary antibody Try bleach and you will destroy everything! René J. --- On Sat, 5/16/09, TF ti...@foxmail.com wrote: From: TF ti...@foxmail.com Subject: [Histonet] Chemicals that inactivate the primary antibody To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Date: Saturday, May 16, 2009, 12:48 PM Hi all, just wonder what kind of treatments/chemicals can complete block the binding of 2nd antibody to binded primary antibody on antigen? I tried HCl , but does not damage all the primaryantibody binding sites - i can still see staining pattern finally. 2009-05-17 TF ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: Re: AW: [Histonet] Chemicals that inactivate the primary antibody
Hi TF, we do IF with two 1.ABs out of mice using a sequential protocol from abcam: www.abcam.com/technical We perform a second blocking step with serum (higher conc. than the first one) after the first Fluorescence was added, do not perform another retrieval procedure (my ABs need the same) and perform the following incubation steps in the dark (we switch the light off and cover the slides after adding the ABs) to prevent fading. Until now, I was sure, this procedure works but maybe the two antigens don't colocalize but it is just a staining artifact ;-) Hope this helps, Frauke Zitat von TF ti...@foxmail.com: Thanks all, Merced M Leiker is right: the double IHC procedure using two primary antibodies from same species. Some expert just sent me their procedure of Heat-interval based inactivation of the primary antibody for first antigen. I am just seeking for a chemical way at room temperature. 2009-05-18 TF 发件人： Rene J Buesa 发送时间： 2009-05-18 22:31:30 收件人： tifei; gu.lang; Merced M Leiker 抄送： histonet 主题： Re: AW: [Histonet] Chemicals that inactivate the primary antibody I am completely confused. IF you have an epitope in the tissue and you react with it an specific antibody that is it. The epitope will have reacted and I do not see any way in which you can add another antibody to that initial epitope. From the reactive point of view, the epitope is blocked to react with another, unless the reaction is not totally specific and there is room for another. I just cannot imagine a way of doing this. René J. --- On Mon, 5/18/09, Merced M Leiker lei...@buffalo.edu wrote: From: Merced M Leiker lei...@buffalo.edu Subject: Re: AW: [Histonet] Chemicals that inactivate the primary antibody To: ti...@foxmail.com, gu.l...@gmx.at Cc: histonet@lists.utsouthwestern.edu Date: Monday, May 18, 2009, 9:42 AM Hi TF and all interested, I think I know what you want, but unfortunately I don't know how to answer your question (it is something I'd like answered myself!!) To re-word for the sake of all interested: You want to perform double-immunofluorescent staining using 2 primaries that were raised in the same species. An additional question for clarification is: Do you want to do this on paraffin or frozen sections? Maybe someone can help figure it out... Merced --On Sunday, May 17, 2009 2:16 AM +0800 TF ti...@foxmail.com wrote: But the heat also damage the fluorescnece... u need specific amplication kit anyway. 2009-05-17 TF 发件人： Gudrun Lang 发送时间： 2009-05-17 02:00:18 收件人： ti...@foxmail.com 抄送： histonet@lists.utsouthwestern.edu 主题： AW: [Histonet] Chemicals that inactivate the primary antibody For doublestaining the primary undergoes denaturation through a second HIER-step. The second secondary ab doesn't bind to the first primary. Therefore the binding sites must have been destroyed by heat in retrieval-buffer. Gudrun -Urspr黱gliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von TF Gesendet: Samstag, 16. Mai 2009 18:48 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] Chemicals that inactivate the primary antibody Hi all, just wonder what kind of treatments/chemicals can complete block the binding of 2nd antibody to binded primary antibody on antigen? I tried HCl , but does not damage all the primaryantibody binding sites - i can still see staining pattern finally. 2009-05-17 TF ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 lei...@buffalo.edu 716-829-6118 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] coverslipping helper
Good Monday morning! This may sound crazy...or not. I have to admit that I have never heard of this device before and I was wondering if any of you out in histoland could tell me what this is and where one might go to get it, if it were available for sale. Here goes: A student who was having a difficult time learning how to coverslip slides in my lab told me that she used to work for a doctor who invented a device to help coverslip. It was like a magnet and just eliminated all bubbles from under the coverslip. Anybody know? Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 happy slicing and dicing and may all your stains work perfectly - Paula Sicurello ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Steel Microtome Blades
Does anyone still use the steel microtome blades that need to be sharpened? We have found some of these blades in our laboratory and will dispose of them, if no one has any use for them. Jeannette B. Wallen, HT (ASCP) Histology Specialist/Histology Team Lead Hennepin County Medical Center Anatomic Pathology/Histology Lab 701 Park Avenue, Mail Code: PL Minneapolis, MN 55415 (612) 873-9108 Office, PL.731 (612) 510-5677 Pager (612) 873-3079 General Histology (612) 904-4629 Fax Email Address: jeannette.wal...@hcmed.orgmailto:jeannette.wal...@hcmed.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] coverslipping helper
Never heard of that, but perhaps she is referring to placing some small weight over the coverslipped section to help eliminate the bubbles by the weight, other than that, that is totally new for me. René J. --- On Mon, 5/18/09, Andrea Grantham algra...@email.arizona.edu wrote: From: Andrea Grantham algra...@email.arizona.edu Subject: [Histonet] coverslipping helper To: HISTONET histonet@lists.utsouthwestern.edu Date: Monday, May 18, 2009, 12:13 PM Good Monday morning! This may sound crazy...or not. I have to admit that I have never heard of this device before and I was wondering if any of you out in histoland could tell me what this is and where one might go to get it, if it were available for sale. Here goes: A student who was having a difficult time learning how to coverslip slides in my lab told me that she used to work for a doctor who invented a device to help coverslip. It was like a magnet and just eliminated all bubbles from under the coverslip. Anybody know? Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 happy slicing and dicing and may all your stains work perfectly - Paula Sicurello ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Alpha-naphthyl-butyrate
Looking for the order number for Alpha-naphthyl-butyrate. I will be using it for the NSE procedure. I spoke with the Fisher 800 number and they were unable to help me. We use Fisher or Sigma products. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Steel Microtome Blades
I also have a number of these blades and an old knife sharpener, which I would like to get to a good home. Esther Peters George Mason University Wallen, Jeannette B wrote: Does anyone still use the steel microtome blades that need to be sharpened? We have found some of these blades in our laboratory and will dispose of them, if no one has any use for them. Jeannette B. Wallen, HT (ASCP) Histology Specialist/Histology Team Lead Hennepin County Medical Center Anatomic Pathology/Histology Lab 701 Park Avenue, Mail Code: PL Minneapolis, MN 55415 (612) 873-9108 Office, PL.731 (612) 510-5677 Pager (612) 873-3079 General Histology (612) 904-4629 Fax Email Address: jeannette.wal...@hcmed.orgmailto:jeannette.wal...@hcmed.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Thermo-Fisher Products
I have been a user of the Sakura Xpress 120 (2 units) for 5+ years and find them to be very reliable w/ excellent performance and they fit into the continuous workflow process exceptionally well. William DeSalvo B.S. HTL (ASCP) Production Manager, Sonora Quest Laboratories Chair Person, NSH Quality Control Committee wdesalvo@hotmail.com Date: Mon, 18 May 2009 10:41:07 -0400 From: cb...@lexclin.com To: lmurp...@aultman.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thermo-Fisher Products CC: We have an Excelsior that is about 5 years old. We have had a lot of issues and have had service too many times in the past year to count. We are looking at replacing it. Perhaps we got a lemon, but I don't think I could recommend it based on our experience. Are there any other processors anyone would like to recommend? Also, how about microwave processors and IHC? Thanks, Carol -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Leann M. Murphy Sent: Monday, May 11, 2009 10:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo-Fisher Products I was just wondering what everyone's thoughts were on thermo products. I have been demo-ing an Exlcelsior Processor and was wondering if anyone had any problems. Let me know. LeAnn Murphy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE OF CONFIDENTIALITY This electronic message, including attachments, is for the sole use of the named recipient and may contain confidential or privileged information protected by the State of Kentucky and/or Federal regulations. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. If you are not the intended recipient and have received this communication in error, please contact the sender and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Hotmail® has a new way to see what's up with your friends. http://windowslive.com/Tutorial/Hotmail/WhatsNew?ocid=TXT_TAGLM_WL_HM_Tutorial_WhatsNew1_052009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] QA Manual Question
What are you trying to track and what do you want to accomplish? Are you going to track and record defects/errors produced in your process, performance of the individuals, quality of the processes or all? This is a big project that will require setting up controls to monitor performance and it would be helpful to know what you now have in place. Date: Fri, 15 May 2009 13:04:41 -0700 From: arvidsonkris...@yahoo.com To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QA Manual Question Hi All...Again. I guess I have a lot of questions lately. I have asked this before but I would like to ask it again. What are people doing for QA/QC? I have been asked by management to write a QA manual. I have some ideas but not really sure where to start. Thanks in advance for any input. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Hotmail® has a new way to see what's up with your friends. http://windowslive.com/Tutorial/Hotmail/WhatsNew?ocid=TXT_TAGLM_WL_HM_Tutorial_WhatsNew1_052009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Alpha-naphthyl-butyrate
Check out the Sigma-Aldrich website at: www.sigmaaldrich.com 91A is part of a kit 90A1 is the select reagent packaged in gelatin capsules. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 Stella Mireles estellamire...@gmail.com 05/18/09 1:16 PM Looking for the order number for Alpha-naphthyl-butyrate. I will be using it for the NSE procedure. I spoke with the Fisher 800 number and they were unable to help me. We use Fisher or Sigma products. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: Re: AW: [Histonet] Chemicals that inactivate the primaryantibody
Hi Frauke, I've read the abcam protocol. They don't indicate what species the ab's are from. Or I've overlooked it. What is the reason, why the second secondary ab doesn't bind to the first primary ab? Gudrun -Ursprüngliche Nachricht- Von: Dr. Frauke Neff [mailto:ne...@staff.uni-marburg.de] Gesendet: Montag, 18. Mai 2009 18:17 An: ti...@foxmail.com Cc: Rene J Buesa; gu.lang; Merced MLeiker; histonet Betreff: Re: Re: AW: [Histonet] Chemicals that inactivate the primaryantibody Hi TF, we do IF with two 1.ABs out of mice using a sequential protocol from abcam: www.abcam.com/technical We perform a second blocking step with serum (higher conc. than the first one) after the first Fluorescence was added, do not perform another retrieval procedure (my ABs need the same) and perform the following incubation steps in the dark (we switch the light off and cover the slides after adding the ABs) to prevent fading. Until now, I was sure, this procedure works but maybe the two antigens don't colocalize but it is just a staining artifact ;-) Hope this helps, Frauke Zitat von TF ti...@foxmail.com: Thanks all, Merced M Leiker is right: the double IHC procedure using two primary antibodies from same species. Some expert just sent me their procedure of Heat-interval based inactivation of the primary antibody for first antigen. I am just seeking for a chemical way at room temperature. 2009-05-18 TF 发件人： Rene J Buesa 发送时间： 2009-05-18 22:31:30 收件人： tifei; gu.lang; Merced M Leiker 抄送： histonet 主题： Re: AW: [Histonet] Chemicals that inactivate the primary antibody I am completely confused. IF you have an epitope in the tissue and you react with it an specific antibody that is it. The epitope will have reacted and I do not see any way in which you can add another antibody to that initial epitope. From the reactive point of view, the epitope is blocked to react with another, unless the reaction is not totally specific and there is room for another. I just cannot imagine a way of doing this. René J. --- On Mon, 5/18/09, Merced M Leiker lei...@buffalo.edu wrote: From: Merced M Leiker lei...@buffalo.edu Subject: Re: AW: [Histonet] Chemicals that inactivate the primary antibody To: ti...@foxmail.com, gu.l...@gmx.at Cc: histonet@lists.utsouthwestern.edu Date: Monday, May 18, 2009, 9:42 AM Hi TF and all interested, I think I know what you want, but unfortunately I don't know how to answer your question (it is something I'd like answered myself!!) To re-word for the sake of all interested: You want to perform double-immunofluorescent staining using 2 primaries that were raised in the same species. An additional question for clarification is: Do you want to do this on paraffin or frozen sections? Maybe someone can help figure it out... Merced --On Sunday, May 17, 2009 2:16 AM +0800 TF ti...@foxmail.com wrote: But the heat also damage the fluorescnece... u need specific amplication kit anyway. 2009-05-17 TF 发件人： Gudrun Lang 发送时间： 2009-05-17 02:00:18 收件人： ti...@foxmail.com 抄送： histonet@lists.utsouthwestern.edu 主题： AW: [Histonet] Chemicals that inactivate the primary antibody For doublestaining the primary undergoes denaturation through a second HIER-step. The second secondary ab doesn't bind to the first primary. Therefore the binding sites must have been destroyed by heat in retrieval-buffer. Gudrun -Urspr�ngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von TF Gesendet: Samstag, 16. Mai 2009 18:48 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] Chemicals that inactivate the primary antibody Hi all, just wonder what kind of treatments/chemicals can complete block the binding of 2nd antibody to binded primary antibody on antigen? I tried HCl , but does not damage all the primaryantibody binding sites - i can still see staining pattern finally. 2009-05-17 TF ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 lei...@buffalo.edu 716-829-6118 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] coverslipping helper
Rene, I think you're correct. In many of the old/antique supply catalogues there are listings for several types of spring coverslip clamping forceps that were used to compress the coverslip while drying the mountant, and to distribute Canada balsam evenly beneath the cover. When I used to make whole mounts of embryos etc and mount them in heavy balsam I used to place lead shot filled ampoules on the coverslips as a leveling weight while they dried. Regards, Jim, J.B.McCormick, M.D. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, May 18, 2009 12:06 PM To: HISTONET; Andrea Grantham Subject: [Histonet] coverslipping helper Never heard of that, but perhaps she is referring to placing some small weight over the coverslipped section to help eliminate the bubbles by the weight, other than that, that is totally new for me. René J. --- On Mon, 5/18/09, Andrea Grantham algra...@email.arizona.edu wrote: From: Andrea Grantham algra...@email.arizona.edu Subject: [Histonet] coverslipping helper To: HISTONET histonet@lists.utsouthwestern.edu Date: Monday, May 18, 2009, 12:13 PM Good Monday morning! This may sound crazy...or not. I have to admit that I have never heard of this device before and I was wondering if any of you out in histoland could tell me what this is and where one might go to get it, if it were available for sale. Here goes: A student who was having a difficult time learning how to coverslip slides in my lab told me that she used to work for a doctor who invented a device to help coverslip. It was like a magnet and just eliminated all bubbles from under the coverslip. Anybody know? Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 happy slicing and dicing and may all your stains work perfectly - Paula Sicurello ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: AW: Re: AW: [Histonet] Chemicals that inactivate the primaryantibody
I tested the sequential IHC protocol...though a bit different. I am using two mouse monoclonal antibody, and Goat-anti Mouse Alexa 488 , 568, respectively. The 2nd secondary Ab will bind to the 1st primary antibody, for sure, with almost 80% background (you can visualize very dark cell morphology in the other channel), and around 10-50% clear co-labeling of two different cell markers. Without any treatment between the 1st and 2nd IHC, I set up this as a control. Maybe, the blocking step Frauke mentioned can greatly reduces co-labeling? 2009-05-19 TF 发件人： Gudrun Lang 发送时间： 2009-05-19 02:50:42 收件人： 'Dr. Frauke Neff' 抄送： histonet 主题： AW: Re: AW: [Histonet] Chemicals that inactivate the primaryantibody Hi Frauke, I've read the abcam protocol. They don't indicate what species the ab's are from. Or I've overlooked it. What is the reason, why the second secondary ab doesn't bind to the first primary ab? Gudrun -Ursprüngliche Nachricht- Von: Dr. Frauke Neff [mailto:ne...@staff.uni-marburg.de] Gesendet: Montag, 18. Mai 2009 18:17 An: ti...@foxmail.com Cc: Rene J Buesa; gu.lang; Merced MLeiker; histonet Betreff: Re: Re: AW: [Histonet] Chemicals that inactivate the primaryantibody Hi TF, we do IF with two 1.ABs out of mice using a sequential protocol from abcam: www.abcam.com/technical We perform a second blocking step with serum (higher conc. than the first one) after the first Fluorescence was added, do not perform another retrieval procedure (my ABs need the same) and perform the following incubation steps in the dark (we switch the light off and cover the slides after adding the ABs) to prevent fading. Until now, I was sure, this procedure works but maybe the two antigens don't colocalize but it is just a staining artifact ;-) Hope this helps, Frauke Zitat von TF ti...@foxmail.com: Thanks all, Merced M Leiker is right: the double IHC procedure using two primary antibodies from same species. Some expert just sent me their procedure of Heat-interval based inactivation of the primary antibody for first antigen. I am just seeking for a chemical way at room temperature. 2009-05-18 TF 发件人： Rene J Buesa 发送时间： 2009-05-18 22:31:30 收件人： tifei; gu.lang; Merced M Leiker 抄送： histonet 主题： Re: AW: [Histonet] Chemicals that inactivate the primary antibody I am completely confused. IF you have an epitope in the tissue and you react with it an specific antibody that is it. The epitope will have reacted and I do not see any way in which you can add another antibody to that initial epitope. From the reactive point of view, the epitope is blocked to react with another, unless the reaction is not totally specific and there is room for another. I just cannot imagine a way of doing this. René J. --- On Mon, 5/18/09, Merced M Leiker lei...@buffalo.edu wrote: From: Merced M Leiker lei...@buffalo.edu Subject: Re: AW: [Histonet] Chemicals that inactivate the primary antibody To: ti...@foxmail.com, gu.l...@gmx.at Cc: histonet@lists.utsouthwestern.edu Date: Monday, May 18, 2009, 9:42 AM Hi TF and all interested, I think I know what you want, but unfortunately I don't know how to answer your question (it is something I'd like answered myself!!) To re-word for the sake of all interested: You want to perform double-immunofluorescent staining using 2 primaries that were raised in the same species. An additional question for clarification is: Do you want to do this on paraffin or frozen sections? Maybe someone can help figure it out... Merced --On Sunday, May 17, 2009 2:16 AM +0800 TF ti...@foxmail.com wrote: But the heat also damage the fluorescnece... u need specific amplication kit anyway. 2009-05-17 TF 发件人： Gudrun Lang 发送时间： 2009-05-17 02:00:18 收件人： ti...@foxmail.com 抄送： histonet@lists.utsouthwestern.edu 主题： AW: [Histonet] Chemicals that inactivate the primary antibody For doublestaining the primary undergoes denaturation through a second HIER-step. The second secondary ab doesn't bind to the first primary. Therefore the binding sites must have been destroyed by heat in retrieval-buffer. Gudrun -Urspr�ngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von TF Gesendet: Samstag, 16. Mai 2009 18:48 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] Chemicals that inactivate the primary antibody Hi all, just wonder what kind of treatments/chemicals can complete block the binding of 2nd antibody to binded primary antibody on antigen? I tried HCl , but does not damage all the primaryantibody binding sites - i can still see staining pattern finally. 2009-05-17 TF ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Cardiovascular Medicine 348 Biomedical Research
[Histonet] looking for Larry Mahler
Hi, Does anyone know how to contact Larry Mahler. Is or was with Scientific Instrument Service in TN.I am looking for some help with a IL MVP I tissue processor. I would like to also find anyone who might know about where to find parts for this older machine. LeRoy Brown HT(ASCP) HTL Everson, WA 98247 360-966-7300 www.histocs.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Slide quality
I think your techs have misplaced a digit. It should only be about 5-10% ammonium chloride. (plenty strong enough for ME thank you.) And no, we don't have IHC problems later. Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Saundra Johnson Sent: Fri 5/8/2009 12:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide quality Hi Bernice, I noticed a post in the histonet archives about using ammonia water to soak. Could I ask your opinion about how ammonia water could effect the IHC staining? I have come to work here and the two techs here soak in 50% ammonia water. That seems way to strong to me. What do you think? Saundra Johnson, HT(ASCP)QIHC Histology Supervisor NeoGenomics Laboratories When Time Matters...And Results Count 12701 Commonwealth Drive, Suite 9 Fort Myers, FL 33913 Phone: (239) 768-0600 Ext. 2229 Fax: (239) 768-0711 This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secured or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. NeoGenomics Laboratories, Suite 5, 12701 Commonwealth Dr, Fort Myers, FL 33913, http://www.neogenomics.org http://www.neogenomics.org/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: heartworm processing protocol
hello, will someone with a tried proven protocol for processing heartworms for paraffin sectioning please share your protocol with me ASAP? My histonet archive search was unsuccessful. Thanks! Atoska ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fixation of Formalin vs Bouin's for IHC
Hi histonetters, Please help. What is difference in procedure for IHC between 10%Formalin fixed tissue and in Bouin's fixed tissue? I used to use 10%FFPE tissue with Citrate buffer Antigen Retrieval for my IHC. Do I have to change my protocol for the Bouin's fixed tissue? Hope for you soon response and thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Special Stainers
I am wanting to explore all my options. I am having some real issues with our automated special stainer and I am considering going to a new system. Can anyone tell me what systems are out on the market that preforms hands-free special stains. All options are open. Vendors welcome to reply. Mike Pence AP Supervisor Great River Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AFB Fites of cytospins
Hello Histonet. Has anyone had experience and good results doing AFB Fites on cytospin slides from BAL's? Do you use the peanut oil/xylene (PO/Z) solution or just 100% peanut oil? Or does the cytospin slide need any peanut oil? After the deparaffinization step, if it can be called that, we are going to stain using a Dako Artisan special stainer. One problem I foresee is that the control will be de-arffinized in the traditional PO/Z while the patient slide may not. Also, I was told that Cytolyte contains alcohol and asked that the slides not be prepared using Cytolyte. Thanks for your comments. Please respond back to the histonet if possible and not my email address. Carrie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
