AW: [Histonet] IHC control debate
Here is another publication: Preservation of Estrogen Receptor in Paraffin Sections, Christine M. Bromley et al; J. Histotech. 17:115, 1994 Gudrun Lang -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Lynette Pavelich Gesendet: Dienstag, 26. Mai 2009 20:16 An: histonet@lists.utsouthwestern.edu; f...@ufl.edu Betreff: [Histonet] IHC control debate Hello, I need help from all of our IHC expert guru's! We have a debate going on about the known positive control blocks. All are FFPE with 8-48 hrs in formalin. All have been tested and have been approved for use as positive controls. We currently cut the positive control from the block as needed. I have learned that some controls do start to deteriorate over time, tonsils for example. But, is there a list, an article, SOMETHING that can guide me so that we can cut some of these controls ahead of time? This would not only save tech time, but would further the use of our controls. In cutting the block, some of the good control is wasted and some positives are hard to find. My argument is that the purchased controls work wonderfully, even after a very long time and our improved detection kits have improved so much over the years, it makes this possible. If there is no published article out there, I implore you experts to please help us in this area. Would also like to hear from the technical experts from our suppliers too! Until then, the debate continues on!!! Thank you!! Lynette ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] LFB and DAB: do they play nice together?
Hello, Sorry to be a pest, but normally responses come quite quick to queries so I'm figuring that maybe my last query never registered (this being the wake of Memorial Day weekend and all...). Has anyone out there tried DAB on sections and thereafter stained with luxol fast blue-cresyl violet? I'm looking at neurogensis in the brain of fish and want to be able to get a good idea of which layers the DAB+ nuclei are lying in. Any other suggestions for a counterstain that can be used on DAB stained sections? I'm adjusting the DAB color with Cobalt Chloride, which should change it from brown to blue-black---maybe not the best idea with LFB. What about when using copper or nickle? Thanks -- Tyrone Genade http://tgenade.freeshell.org email: tgen...@freeshell.org tel: +27-84-632-1925 (c) Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] test
This is a test as I am not receiving email from histonet. MaryAnn Dixon BS, HT(ASCP) Biological Scientist Oncology UF Veterinary Medical Center (352) 392-2226 Ext. 5739 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CJD procedures
We are looking to revamp our procedure for CJD and I would like to see if there are different protocols out there. At this point we are handling the whole process by hand, including melting the paraffin in a melting pot. Do we need a separate oven for melting the paraffin and heating the slides? Thank you Debra Ann Ortiz Chief Medical Technologist The University of Chicago Medical Center Room E-602-A 5841 S. Maryland Avenue Chicago, Il 60637 phone: 773.702.5237 This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CJD procedures
Debra Contact Case Western University. They have National Pathology Survelience Prion Disease site - www.cjdsurveillance.com. This is a excellent resource Best Regards Mari Ann Mailhiot BA HT ASCP Application Specialist/Trainer Leica Microsystems Biosystems Division Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailh...@leica-microsystems.com www.leica-microsystems.com debra.or...@ucho spitals.edu Sent by: To histonet-bounces@ histonet@lists.utsouthwestern.edu lists.utsouthwest cc ern.edu Subject [Histonet] CJD procedures 05/27/2009 11:10 AM We are looking to revamp our procedure for CJD and I would like to see if there are different protocols out there. At this point we are handling the whole process by hand, including melting the paraffin in a melting pot. Do we need a separate oven for melting the paraffin and heating the slides? Thank you Debra Ann Ortiz Chief Medical Technologist The University of Chicago Medical Center Room E-602-A 5841 S. Maryland Avenue Chicago, Il 60637 phone: 773.702.5237 This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CJD procedures
The American Biological Safety Association (ABSA) and its Biosafety Mailing List are two excellent resources for obtaining information on handling CJD materials. American Biological Safety Association (ABSA) http://www.absa.org ABSA - Resources and Tools - E-Mail Groups http://www.absa.org/restool.html http://www.absa.org/resgroups.html ABSA is also useful for any other question about biological safety. Its members have practical knowledge of laboratory operations. :o) Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: michael.o...@fda.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Custom ISH probes
We are interested in having a custom ISH probe prepared using a specific sequence for which we already have the necessary information. Anyone know of a reliable company who does this? Vendors are welcome to contact me. Hermina Hermina M. Borgerink, BA, HT, HTL(ASCP)QIHC Wake Forest University Primate Center Department of Pathology Medical Center Blvd. Winston-Salem, NC 27157 Tel. (336) 716-1538 Fax. (336) 716-1515 e-mail: hborg...@wfubmc.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Melanin / melanocytes staining method tintorial or IHC
Craig, I was searching the archives on the histonet concerning the Dopa Oxidase stain. I have not performed this stain but have done the Schmorls stain. I was wondering if you had a particular method/protocol that you use for this stain? Are there any kits available or is it cheaper to do buying the reagents separately? Any suggestions would be greatly appreciated. Regards, Medea -- Medea J. McGraw BS, HT, HTL(ASCP) CM IHC Research Histotechnologist Phenopath Laboratories Phone: 206-374-9000 x1033 Fax: 206-374-9009 This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Full Time Histology Position Opening in Towson, Md
Hello Histonet, St. Joseph Medical Center in Towson, Md. is looking for a full time Histology Technician for day shift. If interested, please contact Paula Wilder, Histology Supervisor at 410-337-1741. _ Hotmail® has ever-growing storage! Don’t worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutorial_Storage1_052009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: [IHCRG] CD21
Jean, Here is the details from our manual ANTIGEN NAME: CD 21 OTHER NAMES: Clone: 2G9 Isotype: IgG2a DESCRIPTION: Monoclonal antibody to a Prokaryotic recombinant protein corresponding to the external domain of the CD21 molecule. STORAGE CONDITIONS:4-8oC undiluted SUPPLIER:Novocastra NCL-CD21-2G9 PROCEDURE: Methodology:HRP-POLYMER Working Dilution:1/20Bond 1/40 Special Conditions: HIER (pH6 - Citrate) Frozen Section: CONTROL TISSUE: Lymph Node/Tonsil Inbuilt Controls Lymphocytes CLINICAL SIGNIFICANCE: CD21 antigen is a type I integral membrane glycoprotein of molecular weight 140kD. The CD21 molecule, present on mature B cells, is involved in transmitting growth-promoting signals to the interior of the B cell and acts as a receptor for Epstein-Barr virus. The antigen is absent on T-lymphocytes, monocytes and granulocytes. REFERENCES: Ling N R, Brown B and Hardie D (1994) Journal of Immunological Methods. 173: 11-17 Timens W, Boes A, Vos H, et al (1991) Histochemistry. 95: 605-611 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of Taylor, Jean Sent: Thursday, 28 May 2009 3:52 AM To: 'ih...@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [IHCRG] CD21 Hi everyone, We are looking into ordering the CD21 antibody and was wondering what antibody clone labs are using. Thanks! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI 53715 * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
