RE: [Histonet] nuclear bubbling
Sub optimal fixation and as Rene said; drying off at high temperatures. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: 15 July 2009 20:45 To: Histonet Subject: [Histonet] nuclear bubbling Has anyone experienced nuclear bubbling on prostate biopsies? Joyce * CONFIDENTIALITY NOTICE * This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] formalin substitutes - tissue structure
I have no scientific experience with this, but in my opinion it has to make a difference, if the proteins are crosslinked with single methylen-bridges or with longer bridges, that result of more-C-compounds. Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Yak-Nam Wang Gesendet: Mittwoch, 15. Juli 2009 01:32 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] formalin substitutes - tissue structure Dear Histonetters, I have a question about alternatives to formalin fixation and fine changes in tissue structure. We have been obtaining formalin fixed human skin and fat samples from several companies. We use stereological methods to make tissue measurements such as dermal thickness and adipose cell size from sections stained with a variety of basic stains. However, there is now another company that we would like to do obtain more tissue from but they can only provide tissue fixed with a formalin alternative such as FineFix or Prefer. Measurement data collected from formalin and formalin alternative fixed tissue would be used together if we obtained tissue from this other company. From the Histonet archives I see that sometimes the use of formalin alternatives can affect immuno staining, but does anyone know how it would affect fine structure of tissue. My thoughts were that there may be a slight difference in 'shrinkage' that occurs given the main ingredient is ethanol on some of the alternatives, so fat fixed in one of these alternatives would give an erroneously smaller adipose cell size. Any insight would be greatly appreciated. Thank you Yak-Nam Wang University of Washington ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GFP IHC frozen samples
Hi all,
I spent hours and looked at several antibodies on this subject.
Does anybody have a protocol that actually works for GFP staining on
fresh frozen samples or is the signal simply destroyed?
Many thanks
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[Histonet] immuno staining
We use Premiere Charged Slides purchased from Cardinal and/or Mercedes for our immuno stains. We recently purchased the Ventana Ultra and really like it. We dual mount the control and patient. We have recently had several cases where the control was positive but the patient was not. We repeated the stain and the patient was positive the second time. We have had issues with washing but have increased the time in the oven Has anyone else had this problem We don't have a lot of variables that are different Thanks for any input The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Osteoid fluorescence staining
Hey Histonetters, Can anybody tell me a fluorescence stain for osteoid. I need to measure the osteoid volume around/at the surface of bone lacunae with confocal laser scanning microscopy. I heard about basic fuchsin but there the lacunae voids are also stained so i guess it's hard then to distinguish between the void and the osteoid. Thanks for your help. Michael === Michael Kerschnitzki Biomaterials Department Max Planck Institute of Colloids and Interfaces Postal address: MPI KGF Golm, D - 14424 Potsdam, Germany Physical address: Am Mühlenberg 1, D - 14476 Golm Germany Telephone: +49 (331) 567 - 9464 Fax: +49 (331) 567 - 9402 Email: kerschnit...@mpikg.mpg.de === ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job Opening Columbus-Ohio
We currently have 2 openings both for an Anatomic Pathology Technologist. 1 position is Full Time from 3:00pm to 11:30pm and the other is Part Time from 4:00pm-8:00pm. Position Description: provides accessioning, gross examination for surgical specimens, creates reports of tissue gross description, prepares tissues for processing, maintains lab supplies and procurement records, provides inventory summaries and other general lab responsibilities as required. Experience and Qualifications: minimum of 2 year associates degree in science related field or minimum of 24 semester hours (36 quarter hours) of biology, chemistry, physics, and math; training or experience as a tissue prosector desired, certification or experience as a histology technologist desired, certification, training or experience as an anatomic pathology technician desired. If interested please email or fax cover letter and resume to: Attn: Dawn Gullifer Fax 614-293-2102 dawn.gulli...@osumc.edu Dawn Gullifer BS, HT (ASCP) Laboratory Manager OSU Histology Lab, LLC 614-293-0358 office 614-293-0345 lab dawn.gulli...@osumc.edu This e-mail, including attachments, may contain information that is physician-patient privileged, proprietary or otherwise confidential. If you are not the intended recipient or his or her authorized agent, use and disclosure of this message are prohibited. Any dissemination, distribution or copying of this e-mail is prohibited. If you have received this e-mail in error, please notify the sender by replying to this e-mail and immediately delete the message and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Position In NJ
Good Morning Histonet, Allied Search Partners is now accepting resumes for client of NJ. We are accepting the following resumes for permanent/direct hire positions: *Histotechnologists/Histotechnicians * Shifts: *Day, Evening, Night* Location: *Somerville, NJ area* Please submit your resume for prescreening purposes to aly...@alliedsearchpartners.com *All inquiries are always kept confidential* Be sure to visit our website www.alliedsearchpartners.com to submit your job search request, refer a friend for $$Cash Bonus$$, and have your resume reviewed by our career advisors. *Other Positions In New Jersey: *Long Branch, NJ, Denville, NJ Please submit resume to aly...@alliedsearchpartners.com and indicate that you are interested in this position -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HTL and HT Thank all of you
Thank all of you who posted a response to my question about BS degree's and Histotechnologist. I especially appreciate the number of responses that came from seasoned HTL's. Because of your impute I was able to convince my hospital and craft a job description that leaves no one out from consideration for the position. Like may of you, I have been burned in the past by having the experience but not the required credentials to be paid properly for the work that I was doing. Unfortunately, this is not likely to change. The success that the USA has shown in matters of QA and QC accuracy have been attributed to our certification and accreditation processes. The increased sophistication of all aspects of Histotechnology and the advent of molecular testing provide an excellent forum for all of us to lobby our certification agencies to include or create additional certifications for Histotechnologist to take part in Cytogenetic, Molecular Pathology and the like. Sure it may require some extra training or college credit but that's exactly what every other field in clinical laboratory medicine has had to do. Every time I'm exposed to another aspect of special staining or IHC it becomes clearer and clearer that the scientific knowledge is there in our technologists. We just need to rally around that point and begin to have our State and National Histology Associations to present a unified front to ASCP, CAP and the like, for more recognition (and pay) based on the tasks that are already being done. We are getting ready to post a position for a Histotechnologist (HTL) position (Manchester, New Hampshire) that will be the point person in crafting the histology laboratory procedures and process for all aspects of the lab. The expected hire date will be in October. We will not be taking patient specimens until February of 2010 so this person will not be engaged in wet work for some time. As the year progresses we will add 2 HT or above positions and 2 Path Tech (Assistant) positions as well. Thanks again for all your help. Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfe...@cmc-nh.org mailto:sfe...@cmc-nh.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] GFP IHC frozen samples
GFP is not destroyed in frozen sections but the tissue should be PFA fixed
prior to sectioning, ideally.
If you have already prepped your tissues and have no choice but to proceed with
fresh frozen be aware that GFP is soluble and can leach out of fresh frozens.
In addition, in fresh frozen preps it is deleterious to let them dry too much
as GFP will quench when dried.
I suggest you fix your tissue immediately after sectioning and just put it on
the scope (after coverslipping obviously) and see what you see, and go from
there.
For anti-GFP use the rabbit anti-GFP from Molecular Probes/Invitrogen. It works
well in frozen and paraffin, immunofluorescence and chromogenic.
-Original Message-
From: Garry Ashton gash...@picr.man.ac.uk
Date: Thu, 16 Jul 2009 12:01:32
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] GFP IHC frozen samples
Hi all,
I spent hours and looked at several antibodies on this subject.
Does anybody have a protocol that actually works for GFP staining on
fresh frozen samples or is the signal simply destroyed?
Many thanks
This email is confidential and intended solely for the use of the person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author and do not necessarily represent those
of the Paterson Institute for Cancer Research or the University of Manchester.
It may contain information that is privileged confidential within the meaning
of applicable law. Accordingly any dissemination, distribution, copying, or
other use of this message, or any of its contents, by any person other than the
intended recipient may constitute a breach of civil or criminal law and is
strictly prohibited. If you are NOT the intended recipient please contact the
sender and dispose of this e-mail as soon as possible.
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[Histonet] Vacuum embedding.
We have recently bought a new vacuum embedding oven but there is some dispute in the lab as to the correct vacuum pressure we should be using. I won't prejudice the replies by giving you our values, but what are the common vacuum pressures that are being used? Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Biological hood with grossing station
there are a few out there - off the top of my head id say try MOPEC Annieinarabia - yayyy its weekend 2009/7/16 Golden State Acrylic Designs gsacrylicdesi...@gmail.com Is the a source for a biological hood with grossing station othe than (Thermo-Fisher) Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Rectal cancer whole mount technique
I recently received a request from our Cancer Center to provide whole mount evaluations of rectal cancer specimens using the Quirke technique and a sledge microtome. The articles I located describe in depth the role of the surgeon, pathologist and radiologist. However there was almost no information related to the equipment needed, modification to processing programs, microtomy or unique skills needed by the histologist. Is there anyone out there who has or is currently performing this technique and is willing to give me an idea what it take to implement in our lab? I would also be interested in a reference lab that would be able to perform the technical component for our pathologist to interpret either on a temporary or potentially permanent basis. Pamela Horge phor...@fairview.org 2450 Riverside Avenue 3rd Floor East Room M340-8 Minneapolis, MN 55454 Phone: 612-273-2884Pager: 612-899-7036 Fax: 612-273-9124 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 1st Time Attendees to NSH S/C Birmingham
Will you be a 1st time attendee to the NSH Symposium/Convention in Birmingham, Alabama in October? Or have not attended an NSH S/C in at least 5 years? If so - you may be eligible for $500 to attend the symposium and be the recipient of one of nine $500 Newcomer-Newcomer Awards. The 4 requirements are: You are a current member of NSH, you need funding, you will utilize the funds to attend the NSH S/C within the next 2 years, you are a 1st time attendee or have not attended a NSH S/C in at least 5 years. One recipient from each of the 9 NSH regions will be chosen for $500 Newcomer-Newcomer Scholarships. Application deadline is August 1st. Apply online through the NSH website. www.nsh.org Recipients will be notified prior to the 2009 NSH S/C. Feel free to contact me if you have any questions regarding this or any of NSH Scholarships/Awards. Jean Mitchell - NSH Awards Chair ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Vacuum embedding.
Hi Ian, For paraffin wax infiltration, the normally quoted range of vacuum pressures to use varies between 360 mmHg(48 kPa) and 260 mmHg(34.66 kPa). I always reduced the pressure slowly in the first wax to just 360 mmHg to prevent violent 'bumping' due to rapid outgassing of the clearant. Second wax can be taken to 300 mmHg and third fourth waxes to 260 mmHg. Cheers, Bryan - Original Message - From: Ian Montgomery ian.montgom...@bio.gla.ac.uk To: histonet@lists.utsouthwestern.edu Sent: Thursday, July 16, 2009 10:41 AM Subject: [Histonet] Vacuum embedding. We have recently bought a new vacuum embedding oven but there is some dispute in the lab as to the correct vacuum pressure we should be using. I won't prejudice the replies by giving you our values, but what are the common vacuum pressures that are being used? Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] aspiring histotech
Thanks to everyone who took the time to answer. I will explore this through NSH and ASCP. It sound like certification and continuing education are the keys!! Cheers, Loralei On Thu, Jul 16, 2009 at 5:33 AM, Haynes, MaryAnne mahay...@cnmc.org wrote: Loralei Anthony, It has been a long time since I took my HTL exam, but the latest materials from NSH and the Bancroft-Gamble book are well worth it. Pursuing a degree and certification is the best way to advance in your career. I have 2 bachelors, 2 masters degrees, and a doctorate along with 4 ASCP certifications. I am currently an operations manager for a research and a clinical AP laboratory, soon to be advanced to an Executive Director. I am given a salary with perks comparable to faculty and staff physicians. I absolutely love my profession and have never left histology behind for career advancement, but I have certainly expanded what I do. Our clinical AP is combined Cytology, Histology, and IHC with ISH. Our research lab is primarily molecular procedures (PCR etc..) along with IHC, ISH, and Laser Capture Microscopy. Education and certification can get you wherever you want to go. Hope this helps Mary Anne D. Haynes, -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Loralei Dewe Sent: Wednesday, July 15, 2009 18:47 To: Anthony Sandoval Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] aspiring histotech I am interested in knowing this also please!! Loralei On Wed, Jul 15, 2009 at 1:35 PM, Anthony Sandoval sandoval.1...@hotmail.com wrote: Hello histology world! I have been working in a histology lab doing mostly IHC for over a year now and I am interested in taking the HT or HTL exam. I have a B.S. in biology so I can sit for either exam. I was wondering about the pro's and con's of each and any study material that would best help me. Thank you all for the info and this site is a great source of info and inspiration for my chosen career! Anthony _ Insert movie times and more without leaving Hotmail(r). http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tu torial_QuickAdd_062009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hi Jan,
Hi Jan, Thanks for your input! I have two A. S. degrees. One in veterinary nursing/technology and one in histology. And I have an AA where all my electives were biology,chemistry and micro. Then I'll have a BS in veterinary nursing/management. I'm starting a molecular program in January. So, I have a strong skills in courses you mentioned. I'm sure I want to do the R D techniques but not so sure about the management! The other thing about a histology career is learning how vast the field is. Did anyone ever have a counselor in their program that explained the different types of positions? I'm looking forward to attending the NSH convention in October. It will be my first one! Carrie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Quality Stuff
There is NO margin of error acceptable in mislabeling blocks or slides. I expect 100% compliance with this in my department. When you have like specimens all day like derm, you cannot make labeling errors. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Thursday, July 16, 2009 4:15 PM To: histonet Subject: [Histonet] Quality Stuff Hello, I work in a derm lab and we do all the grossing. We hand write on all of our blocks and slides, so you can imagine we have mislabelings from time-to-time. I was wondering if other labs have acceptable limits set for errors such as these, and if so what are they like? I am working on setting standards and corrective actions for errors in the lab. Thank you for any input. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Biological hood with grossing station
Although I really like MOPEK, another source for the East Coast would be TBJ. From: Golden State Acrylic Designs gsacrylicdesi...@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Thursday, July 16, 2009 9:34:56 AM Subject: [Histonet] Biological hood with grossing station Is the a source for a biological hood with grossing station othe than (Thermo-Fisher) Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Quality Stuff
There are no acceptable standards for mistakes. The present tendency of implementing the 6σ method in the lab is to precisely eliminate mistakes, not to set an acceptable limit. René J. --- On Thu, 7/16/09, kristen arvidson arvidsonkris...@yahoo.com wrote: From: kristen arvidson arvidsonkris...@yahoo.com Subject: [Histonet] Quality Stuff To: histonet histonet@lists.utsouthwestern.edu Date: Thursday, July 16, 2009, 5:14 PM Hello, I work in a derm lab and we do all the grossing. We hand write on all of our blocks and slides, so you can imagine we have mislabelings from time-to-time. I was wondering if other labs have acceptable limits set for errors such as these, and if so what are they like? I am working on setting standards and corrective actions for errors in the lab. Thank you for any input. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Hi Jan,
Hi Carrie, The NSH convention in Alabama will also be my first. It has only taken me 30 years to finally get to one. Assuming I don't get lost I hope to see you all there (?turn right at Honolulu, left at Los Angeles, then second exit on the left?) I'll be the short tubby man with the funny accent. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carrie Disbrow Sent: Friday, 17 July 2009 6:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hi Jan, Hi Jan, Thanks for your input! I have two A. S. degrees. One in veterinary nursing/technology and one in histology. And I have an AA where all my electives were biology,chemistry and micro. Then I'll have a BS in veterinary nursing/management. I'm starting a molecular program in January. So, I have a strong skills in courses you mentioned. I'm sure I want to do the R D techniques but not so sure about the management! The other thing about a histology career is learning how vast the field is. Did anyone ever have a counselor in their program that explained the different types of positions? I'm looking forward to attending the NSH convention in October. It will be my first one! Carrie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Hi Jan,
Tony, LOL, someone with a funny accent in Alabama. Something only a local can appreciate. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-598-2792 206-598-7659 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Tony Henwood wrote: Hi Carrie, The NSH convention in Alabama will also be my first. It has only taken me 30 years to finally get to one. Assuming I don't get lost I hope to see you all there (?turn right at Honolulu, left at Los Angeles, then second exit on the left?) I'll be the short tubby man with the funny accent. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carrie Disbrow Sent: Friday, 17 July 2009 6:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hi Jan, Hi Jan, Thanks for your input! I have two A. S. degrees. One in veterinary nursing/technology and one in histology. And I have an AA where all my electives were biology,chemistry and micro. Then I'll have a BS in veterinary nursing/management. I'm starting a molecular program in January. So, I have a strong skills in courses you mentioned. I'm sure I want to do the R D techniques but not so sure about the management! The other thing about a histology career is learning how vast the field is. Did anyone ever have a counselor in their program that explained the different types of positions? I'm looking forward to attending the NSH convention in October. It will be my first one! Carrie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Quality Stuff
I also run a derm lab where we gross and write cassettes. The doctors medical assistants make mistakes every week so I set up a double checking system where one tech accessions and check numbers and writes slides. Then I gross and make one final qc check. this is only possible since I have low volume - not too sure about specific setups for larger lab qc Sent from my Verizon Wireless BlackBerry -Original Message- From: Rene J Buesa rjbu...@yahoo.com Date: Thu, 16 Jul 2009 14:47:23 To: histonethistonet@lists.utsouthwestern.edu; kristen arvidsonarvidsonkris...@yahoo.com Subject: Re: [Histonet] Quality Stuff There are no acceptable standards for mistakes. The present tendency of implementing the 6σ method in the lab is to precisely eliminate mistakes, not to set an acceptable limit. René J. --- On Thu, 7/16/09, kristen arvidson arvidsonkris...@yahoo.com wrote: From: kristen arvidson arvidsonkris...@yahoo.com Subject: [Histonet] Quality Stuff To: histonet histonet@lists.utsouthwestern.edu Date: Thursday, July 16, 2009, 5:14 PM Hello, I work in a derm lab and we do all the grossing. We hand write on all of our blocks and slides, so you can imagine we have mislabelings from time-to-time. I was wondering if other labs have acceptable limits set for errors such as these, and if so what are they like? I am working on setting standards and corrective actions for errors in the lab. Thank you for any input. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] VonKossa's calcium stain
Hi, When doing a VonKossa stain in order to demonstrate calcium in tissue, does it matter much if I use Mayer's hematoxylin instead of Ehrlich's hematoxylin (which takes 6 months to ripen) ? Also, can I simply use homemade scott's tapwater for blueing instead of using a lithium carbonate solution ? Thank you for your help, Karine Cadoret Fish health laboratory manager National Center for Marine Conservation and Resource Sustainability Newnham, TAS Australia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] VonKossa's calcium stain
Karine, Either Hx will do, though I would not have used a haematoxylin since it will lake with the calcium forming a blue stained deposit. I would expect it to mask the silver of the von-kossa stain. I would recommend 1% neutral red, ethylene green or even a light eosin counterstain. The silver stained calcium deposits should then stand out quite well. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of karine cadoret Sent: Friday, 17 July 2009 12:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VonKossa's calcium stain Hi, When doing a VonKossa stain in order to demonstrate calcium in tissue, does it matter much if I use Mayer's hematoxylin instead of Ehrlich's hematoxylin (which takes 6 months to ripen) ? Also, can I simply use homemade scott's tapwater for blueing instead of using a lithium carbonate solution ? Thank you for your help, Karine Cadoret Fish health laboratory manager National Center for Marine Conservation and Resource Sustainability Newnham, TAS Australia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] VonKossa's calcium stain
What is your tissue of interest? Why not do the Von Kossa stain first and then counterstain with MacNeal's tetrachrome. This way you employ the use of a metachromatic stain for the rest of the tissue instead of just a nuclear staining hematoxylin. Jack On Jul 16, 2009, at 9:34 PM, karine cadoret kcado...@amc.edu.au wrote: Hi, When doing a VonKossa stain in order to demonstrate calcium in tissue, does it matter much if I use Mayer's hematoxylin instead of Ehrlich's hematoxylin (which takes 6 months to ripen) ? Also, can I simply use homemade scott's tapwater for blueing instead of using a lithium carbonate solution ? Thank you for your help, Karine Cadoret Fish health laboratory manager National Center for Marine Conservation and Resource Sustainability Newnham, TAS Australia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
