Re: [Histonet] Tissue Processor Advice
Sakura Tissue Tek VIP5!! I have 2 of these sturdy workhorses and am very happy Have been associated with VIP5's for as long as they have been around - wonderful machines! AnnieinAbuDhabi aka.Annie out of Africa 2009/8/5 Igor Deyneko igor.deyn...@gmail.com Dear Histonetters! I need your advice in Tissue Processors. The one we currently use, Tissue Tek VIP 3000, is archaic and has finally died. So we are looking into buying a new one. I know that Thermo and Leica both have processors, as well as new Tissue teks, but I wanted to get opinions if you have a preference of a machine, pros and cons of each. I mostly process tumors, with occasional mouse organs thrown in. Any suggestions will be very helpful! Thank you in advance. Sincerely, Igor Deyneko Infinity Pharmaceuticals Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Anne van Binsbergen (Hope) Abu Dhabi UAE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Clear-Rite 3
Greetings! We have been using Clear-Rite 3 here at our lab, and we are happy with the product. Our supplier says it will be on back-order for some time now. Our crack supply folks are looking for another source. I'm taking another route to find out what products out there are comparable. Are all Xylene Substitutes pretty much the same and there for pretty much interchangable? Are there some to stay away from? Any help is appriciated. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Clear-Rite 3
Bill: Xylene substitutes are not all pretty much the same. There are d-Limonene derivatives (something to stay absolutely away from) and alkane derivatives with many known and unknown constituents. Since you are about to change and not have any preference, your best option both in processing quality and price, would be to get a reliable supplier of mineral spirits (the same you can find at any home improvement store) and use it. René J. --- On Wed, 8/5/09, O'Donnell, Bill billodonn...@catholichealth.net wrote: From: O'Donnell, Bill billodonn...@catholichealth.net Subject: [Histonet] Clear-Rite 3 To: Histonet@lists.utsouthwestern.edu Date: Wednesday, August 5, 2009, 9:11 AM Greetings! We have been using Clear-Rite 3 here at our lab, and we are happy with the product. Our supplier says it will be on back-order for some time now. Our crack supply folks are looking for another source. I'm taking another route to find out what products out there are comparable. Are all Xylene Substitutes pretty much the same and there for pretty much interchangable? Are there some to stay away from? Any help is appriciated. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Clear-Rite 3
I would contact Anatech Ltd. they have a substitute Pro-Par Clearant www.anatechltdusa.com - they are all very knowledgeable and would be able to help you I am sure. I don't use substitutes in my lab at this time, however I would contact them if I were ready to make the switch. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: port...@msu.edu Web: www.humanpathology.msu.edu - Original Message - From: O'Donnell, Bill billodonn...@catholichealth.net To: Histonet@lists.utsouthwestern.edu Sent: Wednesday, August 05, 2009 9:11 AM Subject: [Histonet] Clear-Rite 3 Greetings! We have been using Clear-Rite 3 here at our lab, and we are happy with the product. Our supplier says it will be on back-order for some time now. Our crack supply folks are looking for another source. I'm taking another route to find out what products out there are comparable. Are all Xylene Substitutes pretty much the same and there for pretty much interchangable? Are there some to stay away from? Any help is appriciated. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Clear-Rite 3
Bill, I have used Formula 83 from CBG Biotech for several years now and like it. There is no strong odor, dries fast and is recyclable. http://www.cbgbiotech.com Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, August 05, 2009 9:12 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Clear-Rite 3 Greetings! We have been using Clear-Rite 3 here at our lab, and we are happy with the product. Our supplier says it will be on back-order for some time now. Our crack supply folks are looking for another source. I'm taking another route to find out what products out there are comparable. Are all Xylene Substitutes pretty much the same and there for pretty much interchangable? Are there some to stay away from? Any help is appriciated. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Clear-Rite 3
Sorry that is incorrect as companies also offer a substitute that is an isopar derivative that is not d-limonene and has almost no order. I have used for several years and is very good. A number of suppliers offer it. The isopar product is a petroleum distillate and is a known product although the companies call it a non-aliphatic hydrocarbon. Pam Marcum -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, August 05, 2009 10:01 AM To: Histonet@lists.utsouthwestern.edu; BillO'Donnell Subject: Re: [Histonet] Clear-Rite 3 Bill: Xylene substitutes are not all pretty much the same. There are d-Limonene derivatives (something to stay absolutely away from) and alkane derivatives with many known and unknown constituents. Since you are about to change and not have any preference, your best option both in processing quality and price, would be to get a reliable supplier of mineral spirits (the same you can find at any home improvement store) and use it. René J. --- On Wed, 8/5/09, O'Donnell, Bill billodonn...@catholichealth.net wrote: From: O'Donnell, Bill billodonn...@catholichealth.net Subject: [Histonet] Clear-Rite 3 To: Histonet@lists.utsouthwestern.edu Date: Wednesday, August 5, 2009, 9:11 AM Greetings! We have been using Clear-Rite 3 here at our lab, and we are happy with the product. Our supplier says it will be on back-order for some time now. Our crack supply folks are looking for another source. I'm taking another route to find out what products out there are comparable. Are all Xylene Substitutes pretty much the same and there for pretty much interchangable? Are there some to stay away from? Any help is appriciated. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] re: incomplete sections
From personal experience some possible solutions: * Infiltration issues ( is your paraffin fresh, carry over on reagents) * Blocks being too warm or too wet * Once had this issue when cleaning of the processor wasn't being properly followed. Hope this helps a bit. Heather D. Renko, Histology Supervisor OSF Saint Anthony Medical Center Main Laboratory-Histology 5666 East State Street Rockford, Illinois 61108 815-395-5410 Direct 815-395-5116 Department == The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Clear-Rite 3
I use Formula 83 from CBG. I used Clear-Rite for quite a few years, but Formula 83 works as close to Xylene as I have found. Joyce Cline, H.T. (ASCP) Technical Specialist Hagerstown Medical Lab. 301-665-4980 fax 301-665-4941 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, August 05, 2009 9:12 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Clear-Rite 3 Greetings! We have been using Clear-Rite 3 here at our lab, and we are happy with the product. Our supplier says it will be on back-order for some time now. Our crack supply folks are looking for another source. I'm taking another route to find out what products out there are comparable. Are all Xylene Substitutes pretty much the same and there for pretty much interchangable? Are there some to stay away from? Any help is appriciated. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * CONFIDENTIALITY NOTICE * This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Clear-Rite 3
Hi Rene, Why do you not like the d-limonene solvents? I work with them and the results seem fine. I have to mention that I use an AutoTechnicon Duo and cannot place it in a fume hood to vent the xylene vapors. Without proper ventilation the safety people willnot allow the use of Xylenen. I work in the research unit of a VA hospital and they will not provide larger fume hoods nor buy me a self contained processing sytem. Please let me know what other safe alternatives (ones that can be used out on the bench) are you suggest. Thanks, Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. --- On Wed, 8/5/09, Rene J Buesa rjbu...@yahoo.com wrote: From: Rene J Buesa rjbu...@yahoo.com Subject: Re: [Histonet] Clear-Rite 3 To: Histonet@lists.utsouthwestern.edu, BillO'Donnell billodonn...@catholichealth.net Date: Wednesday, August 5, 2009, 2:01 PM Bill: Xylene substitutes are not all pretty much the same. There are d-Limonene derivatives (something to stay absolutely away from) and alkane derivatives with many known and unknown constituents. Since you are about to change and not have any preference, your best option both in processing quality and price, would be to get a reliable supplier of mineral spirits (the same you can find at any home improvement store) and use it. René J. --- On Wed, 8/5/09, O'Donnell, Bill billodonn...@catholichealth.net wrote: From: O'Donnell, Bill billodonn...@catholichealth.net Subject: [Histonet] Clear-Rite 3 To: Histonet@lists.utsouthwestern.edu Date: Wednesday, August 5, 2009, 9:11 AM Greetings! We have been using Clear-Rite 3 here at our lab, and we are happy with the product. Our supplier says it will be on back-order for some time now. Our crack supply folks are looking for another source. I'm taking another route to find out what products out there are comparable. Are all Xylene Substitutes pretty much the same and there for pretty much interchangable? Are there some to stay away from? Any help is appriciated. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Clear-Rite 3
We also use d-limonene (sold as CitriSolv). We used Hemo-De before. I also would be curious you (and others) do not like d-limonene. Peggy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Va Paula Sicurello Sent: Wednesday, August 05, 2009 12:49 PM To: Rene J Buesa; HistoNet Subject: Re: [Histonet] Clear-Rite 3 Hi Rene, Why do you not like the d-limonene solvents? I work with them and the results seem fine. I have to mention that I use an AutoTechnicon Duo and cannot place it in a fume hood to vent the xylene vapors. Without proper ventilation the safety people willnot allow the use of Xylenen. I work in the research unit of a VA hospital and they will not provide larger fume hoods nor buy me a self contained processing sytem. Please let me know what other safe alternatives (ones that can be used out on the bench) are you suggest. Thanks, Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. --- On Wed, 8/5/09, Rene J Buesa rjbu...@yahoo.com wrote: From: Rene J Buesa rjbu...@yahoo.com Subject: Re: [Histonet] Clear-Rite 3 To: Histonet@lists.utsouthwestern.edu, BillO'Donnell billodonn...@catholichealth.net Date: Wednesday, August 5, 2009, 2:01 PM Bill: Xylene substitutes are not all pretty much the same. There are d-Limonene derivatives (something to stay absolutely away from) and alkane derivatives with many known and unknown constituents. Since you are about to change and not have any preference, your best option both in processing quality and price, would be to get a reliable supplier of mineral spirits (the same you can find at any home improvement store) and use it. René J. --- On Wed, 8/5/09, O'Donnell, Bill billodonn...@catholichealth.net wrote: From: O'Donnell, Bill billodonn...@catholichealth.net Subject: [Histonet] Clear-Rite 3 To: Histonet@lists.utsouthwestern.edu Date: Wednesday, August 5, 2009, 9:11 AM Greetings! We have been using Clear-Rite 3 here at our lab, and we are happy with the product. Our supplier says it will be on back-order for some time now. Our crack supply folks are looking for another source. I'm taking another route to find out what products out there are comparable. Are all Xylene Substitutes pretty much the same and there for pretty much interchangable? Are there some to stay away from? Any help is appriciated. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Clear-Rite 3
The last time I was around a citrus based clearing agent was in the 80's and it gave me severe headaches. I didn't have any problems with it's performance, just the health aspects. I was using a closed processor at the time, but no hood.. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-598-2792 206-598-7659 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Sherwood, Margaret wrote: We also use d-limonene (sold as CitriSolv). We used Hemo-De before. I also would be curious you (and others) do not like d-limonene. Peggy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Va Paula Sicurello Sent: Wednesday, August 05, 2009 12:49 PM To: Rene J Buesa; HistoNet Subject: Re: [Histonet] Clear-Rite 3 Hi Rene, Why do you not like the d-limonene solvents? I work with them and the results seem fine. I have to mention that I use an AutoTechnicon Duo and cannot place it in a fume hood to vent the xylene vapors. Without proper ventilation the safety people willnot allow the use of Xylenen. I work in the research unit of a VA hospital and they will not provide larger fume hoods nor buy me a self contained processing sytem. Please let me know what other safe alternatives (ones that can be used out on the bench) are you suggest. Thanks, Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. --- On Wed, 8/5/09, Rene J Buesa rjbu...@yahoo.com wrote: From: Rene J Buesa rjbu...@yahoo.com Subject: Re: [Histonet] Clear-Rite 3 To: Histonet@lists.utsouthwestern.edu, BillO'Donnell billodonn...@catholichealth.net Date: Wednesday, August 5, 2009, 2:01 PM Bill: Xylene substitutes are not all pretty much the same. There are d-Limonene derivatives (something to stay absolutely away from) and alkane derivatives with many known and unknown constituents. Since you are about to change and not have any preference, your best option both in processing quality and price, would be to get a reliable supplier of mineral spirits (the same you can find at any home improvement store) and use it. René J. --- On Wed, 8/5/09, O'Donnell, Bill billodonn...@catholichealth.net wrote: From: O'Donnell, Bill billodonn...@catholichealth.net Subject: [Histonet] Clear-Rite 3 To: Histonet@lists.utsouthwestern.edu Date: Wednesday, August 5, 2009, 9:11 AM Greetings! We have been using Clear-Rite 3 here at our lab, and we are happy with the product. Our supplier says it will be on back-order for some time now. Our crack supply folks are looking for another source. I'm taking another route to find out what products out there are comparable. Are all Xylene Substitutes pretty much the same and there for pretty much interchangable? Are there some to stay away from? Any help is appriciated. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] please remove me from list
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RE: [Histonet] please remove me from list
Unsubscribe me Please. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of zippedinsunsh...@aol.com Sent: Wednesday, August 05, 2009 2:09 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] please remove me from list ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Disclaimer: The HIPAA Final Privacy Rule requires covered entities to safeguard certain Protected Health Information (PHI) related to a person's healthcare. Information being faxed to you may include PHI after appropriate authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain PHI in a safe and secure manner. You may not re-disclose without additional patient consent or as required by law. Unauthorized re-disclosure or failure to safeguard PHI could subject you to penalties described in federal (HIPAA) and state law. If you the reader of this message are not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, please notify us immediately and destroy the related message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Clear-Rite 3
My answer is in the attached article I just published. Please read the d-Limonene discussion seciton. René J. --- On Wed, 8/5/09, Sherwood, Margaret msherw...@partners.org wrote: From: Sherwood, Margaret msherw...@partners.org Subject: RE: [Histonet] Clear-Rite 3 To: Va Paula Sicurello vapat...@yahoo.com, Rene J Buesa rjbu...@yahoo.com, HistoNet histonet@lists.utsouthwestern.edu Date: Wednesday, August 5, 2009, 1:23 PM We also use d-limonene (sold as CitriSolv). We used Hemo-De before. I also would be curious you (and others) do not like d-limonene. Peggy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Va Paula Sicurello Sent: Wednesday, August 05, 2009 12:49 PM To: Rene J Buesa; HistoNet Subject: Re: [Histonet] Clear-Rite 3 Hi Rene, Why do you not like the d-limonene solvents? I work with them and the results seem fine. I have to mention that I use an AutoTechnicon Duo and cannot place it in a fume hood to vent the xylene vapors. Without proper ventilation the safety people willnot allow the use of Xylenen. I work in the research unit of a VA hospital and they will not provide larger fume hoods nor buy me a self contained processing sytem. Please let me know what other safe alternatives (ones that can be used out on the bench) are you suggest. Thanks, Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. --- On Wed, 8/5/09, Rene J Buesa rjbu...@yahoo.com wrote: From: Rene J Buesa rjbu...@yahoo.com Subject: Re: [Histonet] Clear-Rite 3 To: Histonet@lists.utsouthwestern.edu, BillO'Donnell billodonn...@catholichealth.net Date: Wednesday, August 5, 2009, 2:01 PM Bill: Xylene substitutes are not all pretty much the same. There are d-Limonene derivatives (something to stay absolutely away from) and alkane derivatives with many known and unknown constituents. Since you are about to change and not have any preference, your best option both in processing quality and price, would be to get a reliable supplier of mineral spirits (the same you can find at any home improvement store) and use it. René J. --- On Wed, 8/5/09, O'Donnell, Bill billodonn...@catholichealth.net wrote: From: O'Donnell, Bill billodonn...@catholichealth.net Subject: [Histonet] Clear-Rite 3 To: Histonet@lists.utsouthwestern.edu Date: Wednesday, August 5, 2009, 9:11 AM Greetings! We have been using Clear-Rite 3 here at our lab, and we are happy with the product. Our supplier says it will be on back-order for some time now. Our crack supply folks are looking for another source. I'm taking another route to find out what products out there are comparable. Are all Xylene Substitutes pretty much the same and there for pretty much interchangable? Are there some to stay away from? Any help is appriciated. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Helicobacter control slides
Kathy Maddox in Lake Charles, Louisiana asks: I need to buy some Helicobacter Pylori special stain control slides. The ones I have previously bought were from rat tissue and our pathologists don't like them. Can anyone suggest a company that I can purchase them from? Most of the pathology services I've worked with use known positives from their own cases. (Once in a great while you'll see a gastrectomy specimen that provides a lifetime supply.) Obviously the material should be positive with the stain you use (dye? silver? IHC?) I'd trust animal tissue for a dye stain, definitely not for an immunostain, since there are many species of Helicobacter. The two species that infect humans, H. pylori and H. heilmannii )= Gastrospirillum hominis) both mark with the standard IHC reagents. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Xylene substitutes
William (Bill) O'Donnell, HT (ASCP) QIHC, Lead Histologist, Good Samaritan Hospital, Kearney, Nebraska, notes that Clear-Rite 3 is at least temporarily unavailable, and he is looking for a substitute. (Anybody know what's going on with Clear-Rite 3?) Clear-Rite 3 is an aliphatic (rather than a limonene). Aliphatics can't always be substituted for each other - they are chemically different from each other - each has its own flammability characteristics (flash point) and (if you're recovering solvent using a spinning band still) its own distillation routine. If you change, you may want to stay with the product you change to. Your purchasing people need to be aware that they are not to change brands on you. I just worked in a lab that has a Leica ASP300S processor (which they like, by the way). The people there have been told that they may use only one particular aliphatic with it, one I hadn't heard of before: Sub-X aliphatic hydrocarbon isoparaffinic oil CAS 6472-48-9DOT petroleum distillates, NOS, 3 class 3 flammable liquid Flash point 106 F (41 C) Anybody know if this is actually the case? Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Xylene substitutes (UNCLASSIFIED)
Classification: UNCLASSIFIED Caveats: NONE I have 2 Leica ASP 300 tissue processors. I use Thermo Scientific Shandon Xylene Substitute (formally known as Histosolv). I have used this for many years with excellent results. I have never had any problems using this in the processor. We had 2 very old VIP tissue processors before we got the Leica processors and used Histosolv in those processors also. I have never heard of Sub-X. Diane C. Gladney, HT (ASCP) Supervisor, Anatomical Pathology Moncrief Army Community Hospital Dept. of Pathology 4500 Stuart St. FT. Jackson, SC 29207 Email: diane.glad...@amedd.army.mil Phone: 803-751- 2530 FAX: 803-751-7829 DSN: 734-2530 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Wednesday, August 05, 2009 4:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Xylene substitutes William (Bill) O'Donnell, HT (ASCP) QIHC, Lead Histologist, Good Samaritan Hospital, Kearney, Nebraska, notes that Clear-Rite 3 is at least temporarily unavailable, and he is looking for a substitute. (Anybody know what's going on with Clear-Rite 3?) Clear-Rite 3 is an aliphatic (rather than a limonene). Aliphatics can't always be substituted for each other - they are chemically different from each other - each has its own flammability characteristics (flash point) and (if you're recovering solvent using a spinning band still) its own distillation routine. If you change, you may want to stay with the product you change to. Your purchasing people need to be aware that they are not to change brands on you. I just worked in a lab that has a Leica ASP300S processor (which they like, by the way). The people there have been told that they may use only one particular aliphatic with it, one I hadn't heard of before: Sub-X aliphatic hydrocarbon isoparaffinic oil CAS 6472-48-9DOT petroleum distillates, NOS, 3 class 3 flammable liquid Flash point 106 F (41 C) Anybody know if this is actually the case? Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Classification: UNCLASSIFIED Caveats: NONE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: working with mouse liver
Hi Teri, We have been discussing this option and left it at that. I have noticed that the livers, with more lipids look much closer to the textbook images. Michelle, Could it be what you are seeing is normal healthy (actively feeding) liver in mice and the missing cytoplasm is where the glycogen stores are/were? Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
