Re: [Histonet] COX-2
It was in USA Today, but will be published in this week's issue of JAMA. RWC Richard W. Cartun, Ph.D. Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Lynette Pavelich lpave...@hurleymc.com 8/28/2009 2:37 PM Could you share the source of the study? thank you, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: lpave...@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 Richard Cartun rcar...@harthosp.org 08/28/09 2:32 PM There was a recent study that showed aspirin affected the growth of one type of colorectal cancer; that which overexpresses COX-2. If this holds up, get ready to start doing a lot of COX-2 IHC. Does anyone have a good mAb to COX-2 that works on formalin-fixed, paraffin-embedded tissue that they would recommend? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] h.pylori silver staining
Shahram: Under separate cover I am sending you the procedure. René J. --- On Sat, 8/29/09, Shahram Sabeti sabeti_shah...@yahoo.com wrote: From: Shahram Sabeti sabeti_shah...@yahoo.com Subject: [Histonet] h.pylori silver staining To: histonet@lists.utsouthwestern.edu Date: Saturday, August 29, 2009, 3:48 AM hello dear colleagues i have heard of a new modified silver staining method for H.pylori.do you know anything about it and it's methology.please inform me if possible. yours sabeti ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: SPAM-LOW: Re: [Histonet] COX-2
Richard, I did a lot of work on cox2 a while ago on ffpe tissue and I was not ever able to get results I was satisfied with. I would be really interested in this as well, sometimes it just takes finding the right clone and the right pretreatment, I am not there yet with cox2 even though several people gave me their protocols I could not get them to work well. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Saturday, August 29, 2009 8:14 AM To: Lynette Pavelich; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: Re: [Histonet] COX-2 It was in USA Today, but will be published in this week's issue of JAMA. RWC Richard W. Cartun, Ph.D. Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Lynette Pavelich lpave...@hurleymc.com 8/28/2009 2:37 PM Could you share the source of the study? thank you, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: lpave...@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 Richard Cartun rcar...@harthosp.org 08/28/09 2:32 PM There was a recent study that showed aspirin affected the growth of one type of colorectal cancer; that which overexpresses COX-2. If this holds up, get ready to start doing a lot of COX-2 IHC. Does anyone have a good mAb to COX-2 that works on formalin-fixed, paraffin-embedded tissue that they would recommend? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: SPAM-LOW: [Histonet] Best IHC Stainer
I agree with you Amos, but some instruments are more friendly to using other's antibodies and detections than others. In my opinion the Dako autostainer, Leica Bond and the BC IHC autostainers are very good open systems, and even some of them are better at supporting animal and human research. Patsy Patsy Ruegg, HT(ASCP)QIhc IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Friday, August 28, 2009 5:49 PM To: raha...@serha.ca; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Best IHC Stainer Hi Randi, The statement that a stainer is only as good as it's antibodies is nails on a chalk board to me. You can get a good stainer and if the company has bad antibodies ... *change them!* No one company can do it all. If they tell you otherwise RUN they are lieing to you. The definition of a good instrument is one that allows you the flexability to use whatever antibodies you need to use. If you need to run an antibody that isn't offered by the company that sells your instrument, there are thousands of other antibody companies to choose from. If the antibody requires the use of species of secondary that is not offered by the company and the company doesn't allow you to use something else, it is not worth using. Instrumentation is a function of good engineering and software programming. It has little if anything to do with the biochemistry of antibody applications. I really don't mean to sound sharp here, but there are too many people that think that the company that sells their instrument is the only company that they can use. If this is the case with any instrument the customer got shafted in buying the instrument. Amos Message: 6 Date: Fri, 28 Aug 2009 10:57:44 -0300 From: Hayes, Randi (R1SE) raha...@serha.ca Subject: [Histonet] Best IHC Stainer To: histonet@lists.utsouthwestern.edu Message-ID: cdf8c140352fb04a913cf61753056bd30378d...@rhaex1.rha-rrs.ca Content-Type: text/plain; charset=utf-8 A stainer is only as good as its antibodies. We've been less than impressed with the quality of Cell Marque antibodies as well as their customer service. Contact me if you want specifics. What I would suggest is when looking for the best, look for the antibodies that rank the most robust on external EQA surveys (UK Nequas, NordiQC as examples) and go with a system that gives you the flexibility (within reasonable cost) to use the most reliable antibodies. We are Ventana XT users and have been for over 5 years. It provides good staining but with little flexibility to use other vendors antibodies unless you want to completely blow your budget. This forces you to use Cell Marque products and I've already shared my opinion on that. Randi Hayes Histology Supervisor The Moncton Hospital Regional Health Authority B Moncton, NB ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Dear All, we have got a new leica multistainer ST 5020.we are using it for both HE and as well as PAP.Out of 34 station i have only 15 station for HE.Is anybody having a Staining protocol for HE which fits in 15 station with 2 water wash bath. Aazath Technical Officer Apollo Hospitals Chennai India _ Log on to MSN India for a lowdown on what’s hot in the world today http://in.msn.com___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] (no subject)
Hi Aazath, This is our HE protocol, it's for 16 stations. And I'm interested in your PAP-protocol. Would you be so nice and send it to me? 25 min oven 2 x 5 min Xylol-substitute 1 x 1 min 96% 1 x 1 min 80% 1 x 1 min 50% 1 x 1 min dest. water 1 x 10 min Mayers Hemalaun (exact) 1 x 5 min tapwater running 1 x 30 sek 2% Eosin in water (exact) 1 x 30 sek tapwater running (exact) 1 x 30 sek 96% (exact) 3 x 1 min 100% 2 x Xylol-substitute Coverslipper The times without exact are minimum, dip is on with all stations. Alcohols and Xylol-subst. are changed every day. Hope this helps Gudrun Lang -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Aazath Raj Gesendet: Samstag, 29. August 2009 19:51 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] (no subject) Dear All, we have got a new leica multistainer ST 5020.we are using it for both HE and as well as PAP.Out of 34 station i have only 15 station for HE.Is anybody having a Staining protocol for HE which fits in 15 station with 2 water wash bath. Aazath Technical Officer Apollo Hospitals Chennai India _ Log on to MSN India for a lowdown on whats hot in the world today http://in.msn.com___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] h.pylori silver staining
A Warthin-Starry procedure works great. The kit and procedure on the DAKO Artisan is fast, reliable and provides good results. William DeSalvo, B.S., HTL(ASCP) Date: Sat, 29 Aug 2009 07:32:10 -0700 From: rjbu...@yahoo.com To: histonet@lists.utsouthwestern.edu; sabeti_shah...@yahoo.com Subject: Re: [Histonet] h.pylori silver staining CC: Shahram: Under separate cover I am sending you the procedure. René J. --- On Sat, 8/29/09, Shahram Sabeti sabeti_shah...@yahoo.com wrote: From: Shahram Sabeti sabeti_shah...@yahoo.com Subject: [Histonet] h.pylori silver staining To: histonet@lists.utsouthwestern.edu Date: Saturday, August 29, 2009, 3:48 AM hello dear colleagues i have heard of a new modified silver staining method for H.pylori.do you know anything about it and it's methology.please inform me if possible. yours sabeti ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Hotmail® is up to 70% faster. Now good news travels really fast. http://windowslive.com/online/hotmail?ocid=PID23391::T:WLMTAGL:ON:WL:en-US:WM_HYGN_faster:082009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] EM processing
I have never heard of an EM lab making their own resin for embedding. We almost all buy the resin kits or the separate components to make either a Epon 812 equivalent or Spurrs. The amount of time it takes to process is usually tissue dependent. On average, it takes one day to process (if the tissue is 1mm cubed or less) and 2 days in the oven for the resin to polymerize. You can speed things up if you microwave process. If you have any questions, please feel free to contact me. Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. --- On Sat, 8/29/09, histopa...@aol.com histopa...@aol.com wrote: From: histopa...@aol.com histopa...@aol.com Subject: [Histonet] EM processing To: histonet@lists.utsouthwestern.edu Date: Saturday, August 29, 2009, 3:36 AM Does your lab make their own plastic resin to process and embed specimens or do you buy it commercially? What is the average time it take to process a specimen? Thanks in advance to all who respond. P.Eneff OU Medical Center Oklahoma City, OK ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] COX-2
In the meantime, you can google COX-2 aspirin colorectal and get caught up on the various articles written on the topic in the recent past. Jan Shivers UMN VDL On Aug 29 2009, Richard Cartun wrote: It was in USA Today, but will be published in this week's issue of JAMA. RWC Richard W. Cartun, Ph.D. Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Lynette Pavelich lpave...@hurleymc.com 8/28/2009 2:37 PM Could you share the source of the study? thank you, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: lpave...@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 Richard Cartun rcar...@harthosp.org 08/28/09 2:32 PM There was a recent study that showed aspirin affected the growth of one type of colorectal cancer; that which overexpresses COX-2. If this holds up, get ready to start doing a lot of COX-2 IHC. Does anyone have a good mAb to COX-2 that works on formalin-fixed, paraffin-embedded tissue that they would recommend? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] h.pylori silver staining
And to that we add the Alcian blue, HE modification portion of the Genta stain procedure. Works perfectly. Joyce -Original Message- From: histonet-boun...@lists.utsouthwestern.edu on behalf of WILLIAM DESALVO Sent: Sat 8/29/2009 2:38 PM To: rjbu...@yahoo.com; histonet; sabeti_shah...@yahoo.com Subject: RE: [Histonet] h.pylori silver staining A Warthin-Starry procedure works great. The kit and procedure on the DAKO Artisan is fast, reliable and provides good results. William DeSalvo, B.S., HTL(ASCP) Date: Sat, 29 Aug 2009 07:32:10 -0700 From: rjbu...@yahoo.com To: histonet@lists.utsouthwestern.edu; sabeti_shah...@yahoo.com Subject: Re: [Histonet] h.pylori silver staining CC: Shahram: Under separate cover I am sending you the procedure. René J. --- On Sat, 8/29/09, Shahram Sabeti sabeti_shah...@yahoo.com wrote: From: Shahram Sabeti sabeti_shah...@yahoo.com Subject: [Histonet] h.pylori silver staining To: histonet@lists.utsouthwestern.edu Date: Saturday, August 29, 2009, 3:48 AM hello dear colleagues i have heard of a new modified silver staining method for H.pylori.do you know anything about it and it's methology.please inform me if possible. yours sabeti ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Hotmail® is up to 70% faster. Now good news travels really fast. http://windowslive.com/online/hotmail?ocid=PID23391::T:WLMTAGL:ON:WL:en-US:WM_HYGN_faster:082009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
